collecting processing banking and using cord blood stem cells for regenerative medicine
báo cáo khoa học: "Effect of anti-CD52 antibody alemtuzumab on ex-vivo culture of umbilical cord blood stem cells" doc
... culture for day 35 CD34+ cells To study the long term effects of alemtuzumab on hematopoietic progenitors and stem cells, cells were cultured for up to 35 days and CD34+ cells were isolated using ... umbilical cord blood? Blood 2003, 101(11):4233-4244 Huang S, Law P, Young D, Ho AD: Candidate hematopoietic stem cells from fetal tissues, umbilical cord blood vs adult bone marrow and mobilized ... umbilical cord blood for clinical transplantation Curr Stem Cell Res Ther 2007, 2(4):324-335 Hofmeister CC, Zhang J, Knight KL, Le P, Stiff PJ: Ex vivo expansion of umbilical cord blood stem cells for...
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Báo cáo khoa học nông nghiệp " Processing, preservation and using wood of plantation forests " potx
... trend towards using acacia for furniture, preservative treatment is potentially not an issue for most sawmills Occupational Health and Safety is a key issue for rural sawmills Fortunately the ... the Forest Research Group in Hanoi and the major forestry universities located in Hanoi and Ho Chi Mihn City) should have a focus not just on research but investigating and implementing systems ... therefore a prerequisite The heartwood of acacias has some durability and therefore preservative treatment for major uses such as furniture, joinery, flooring and packaging, (the major end uses for...
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Báo cáo hóa học: "Early combined treatment with sildenafil and adipose-derived mesenchymal stem cells preserves heart function in rat dilated cardiomyopathy" doc
... cells and the host tissue As compared with embryonic stems cells and bone marrow-derived mesenchymal stem cells, adiposederived mesenchymal stem cells (ADMSCs) have the distinct advantages of being ... J, Ratajczak MZ: Trafficking of normal stem cells and metastasis of cancer stem cells involve similar mechanisms: pivotal role of the SDF-1-CXCR4 axis Stem Cells 2005, 23:879-894 Kee HJ, Sohn IS, ... Translational Medicine 2010, 8:88 http://www.translational -medicine. com/content/8/1/88 The oxidative stress in mitochondria did not differ between groups and 5, groups and 4, and groups and on day...
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Báo cáo hóa học: "Hybrid approach of ventricular assist device and autologous bone marrow stem cells implantation in end-stage ischemic heart failure enhances myocardial reperfusion" doc
... circulatory support (Figure 1) A stem cells injectate including a mixed population of endothelial progenitor cells (CD133 + ), haematopoietic stem cells (CD34 + ), and Figure A full range of cardiac ... with LVAD and stem cells implantation; enhanced myocardial reperfusion improves prognosis Attempts to improve cardiac performance in chronic ischemic HF patients using cell transplantation and mechanical ... analysis and interpretation GK Collection and assembly of data AD Collection and assembly of data AK Data analysis and interpretation, collection and assembly of data CP Conception and design,...
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báo cáo hóa học:"Transplantation of selected or transgenic blood stem cells – a future treatment for HIV/AIDS?" pot
... [12] However, this source of stem cells has not been used for transplantation in HIV patients yet Cord blood may emerge as an important source of CCR5-negative stem cells because they have been ... that cord stem cell transplantation in adults currently requires more than one cord blood unit Donor recruitment A major issue concerning the supply of CCR5-negative stem cells is the donor information ... could be tested for CCR5-delta32 before costly confirmatory HLA typing is undertaken In an effort to facilitate CCR5-targeted stem cell therapy, Chow et al established a cord blood bank with...
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Báo cáo y học: "An in vitro study investigating the survival and phenotype of mesenchymal stem cells following injection into nucleus pulposus tissue" pot
... Immunofluorescence and immunohistochemistry Immunofluorescence and immunohistochemistry were both performed for aggrecan, Sox-9, and types I, II and X collagen, and immunohistochemistry was performed for caspase ... metabolic activity; for immunohistochemistry for caspase to identify the presence of apoptotic cells; for immunofluorescence and immunohistochemistry for aggrecan, Sox-9 and types I, II and X collagen ... phenotype and direct the cells to form a new and appropriate matrix Materials and methods Mesenchymal stem cell source and extraction Bone marrow samples were received from two patients (aged 66 and...
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Báo cáo y học: "Transcriptional profiles discriminate bone marrow-derived and synovium-derived mesenchymal stem cells" ppsx
... I and class II expression by IFN in adherent cells (a) One example of basal and IFN-γ-induced expression of HLA-A, HLA-B, cells HLA-C (left) and HLA-DR (right) molecules on mesenchymal stem cells ... embryonic stem cells [40] The maintenance of pluripotency of embryonic stem cells could involve Wnt signaling and could occur through a crosstalk between the transforming growth factor beta/activin and ... ± 16% and 36 ± 17% for BM-derived and synoviumderived cells, respectively) and their mean fluorescence were increased (Fig 2b) However, no statistically significant difference between cells isolated...
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Báo cáo y học: "Hypoxic conditions increase hypoxia-inducible transcription factor 2α and enhance chondrogenesis in stem cells from the infrapatellar fat pad of osteoarthritis patients" pps
... mesenchymal stem cells) , and for CD29 (β1 integrin) The cells stained poorly for LNGFR and STRO-1 (markers on freshly isolated bone marrow stem cells) and sparsely for 3G5 (marker for vascular ... bone marrow-derived stem cells suggested that 2% oxygen favoured more primitive stem cells with higher colony-forming unit capability and stronger expression of stem cell genes, and that when switched ... characterisation and flow cytometry of the IPFP cell population showed a similar staining pattern to that of bone marrow-derived stem cells, and although they stained poorly for STRO-1 and for LNGFR,...
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Báo cáo y học: "Sphingosine-1-phosphate promotes the differentiation of human umbilical cord mesenchymal stem cells into cardiomyocytes under the designated culturing conditions" pdf
... neurons, and endothelial cells[ 1-3] Given these characteristics, particularly the plasticity and developmental flexibility, UC stromal cells are now considered an alternative source of stem cells and ... Umbilical Cord Stroma with Regard to the Source of Fetus-Derived Stem Cells Stem cells 2007, 25:2886-2895 Wu KH, Mo XM, Zhou B, Lu SH, Yang SG, Liu YL, Han ZC: Cardiac potential of stem cells from ... morphology and immunostaining with cardiac myocyte specific markers Immunocytochemistry The medium was first removed and the cells were washed twice with PBS, fixed for 30 with 4% paraformaldehyde Cells...
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Báo cáo y học: "Therapeutic potential of human umbilical cord mesenchymal stem cells in the treatment of rheumatoid arthritis" pps
... successfully isolated and expanded from all the umbilical cords They had a fibroblast-like morphology, uniformly negative for CD14, CD45, CD34 and HLA-DR, but positive for CD44, CD73, CD90 and CD29 (Figure ... of proliferative and multilineage differentiation potential of human mesenchymal stem cells derived from umbilical cord and bone marrow Stem Cells 2007, 25:1384-1392 Weiss ML, Anderson C, Medicetty ... using the following parameters Sections were analyzed microscopically for the degree of inflammation and for cartilage and bone destruction according to the method reported previously [26], using...
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Báo cáo y học: "Identification and isolation of embryonic stem cells in reproductive endocrinology: theoretical protocols for conservation of human embryos derived from in vitro fertilization" ppsx
... on to fresh feeder cells for confirmation and further analysis; no cells were frozen This work carries forward a theoretical approach suggested more than a decade ago [12], and demonstrates that ... exposing the ICM for disaggregation and plating on a feeder cell layer for further culture Importantly, this disruptive process renders the embryo non-viable and unsuitable for in utero transfer ... destined for transfer or cryopreservation We investigated the efficacy of a novel methodology with murine embryos that failed to meet viability standards, and were therefore unsuitable for transfer...
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Soluble mediator profiles of cord blood mononuclear cells in early onset childhood allergic disorders
... 4.2 Materials and methods 78 4.2.1 Preparation and storage of cord blood mononuclear cells (CBMCs) and peripheral blood mononuclear cells (PBMC) 78 4.2.2 Stimulation of cord blood cells with lipopolysaccharide ... from cord blood mononuclear cells in the serum-free AIM-V medium 87 4.3.4 Comparison of cytokine secretion profiles between cord blood mononuclear cells (CBMCs) and peripheral blood mononuclear cells ... measure cytokines from un-stimulated cord blood cells, some from stimulated cord blood cells and other studies measure the cytokine from the serum/plasma of the cord blood samples Furthermore, while...
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Differentiation and derivation of lineage committed chondroprogenitors and chondrogenic cells from human embryonic stem cells for cartilage tissue engineering and regeneration
... relief of symptoms Stem cells represent a promising cell source for cartilage repair and can be derived from two major sources: mesenchymal stem cells (MSCs) and embryonic stem cells (ESCs) (Lee ... derived and demonstrated to be expandable, homogenous and unipotent in differentiation potential These cells were functional with ability to form cartilaginous tissue in the pellet system, and stable ... controlling expansion and differentiation so as to yield a safe, scalable and functional cell source for cartilage repair Chondrocytes, fibroblasts, stem cells, and genetically modified cells have all...
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A role for chondroitin sulfate proteoglycan in regulating the survival and growth of neural stem cells
... maintain these cells in an undifferentiated, quiescent state For stem cells to be activated, the niche environment must change (Fuchs, 2007) 1.4.3 Stem cells and cancer Stem cells and cancer cells share ... The stem cell concept 1.3 Symmetrical and asymmetrical division 1.4 Types of stem cells 1.4.1 Embryonic stem cells 1.4.2 Somatic stem cells 1.4.3 Stem ... and bones cannot be replaced after injury However, some tissues such as the skin and blood are constantly being replaced, and this requires a specialized population of cells, the stem cells Stem...
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Design of artificial microenvironment for cord blood CD34+ cells expansion ex vivo
... every 100,000 cells in the marrow is a long-term, blood- forming stem cell; other cells present include stroma cells, blood progenitor cells, and mature and maturing white and red blood cells B: PB ... weeks for red blood cells, and up to several years for memory T cells Thus, a vast amount of blood cells must be permanently produced In normal adults, the body produces about 2.5 billion red blood ... lymphoid cells (T, B, and natural killer cells) and myeloid cells (granulocytes, monocytes, erythrocytes, and megakaryocytes) All blood cells have a limited lifespan: several hours for granulocytes,...
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Gene targeting in human pluripotent cell derived neural stem cells for the study and treatment of neurological disorders
... Introduction 1.1 Stem cells Stem cells have the remarkable potential to differentiate into specialized cells and thereby are key factors for the development of the whole organism All types of stem cells ... 1.1 Stem cells 1.1.1 Human pluripotent stem cells 1.1.2 In vitro differentiation potential of human pluripotent and neural stem cells 1.2 Candidate ... Reynolds and Weiss, 1992; Urbach et al., 2004) Neural stem cells of this process can give rise to neurons and glia, the two lineages most cells of the central nervous system belong to, and are therefore...
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Transplantation of mesenchymal stem cells for the treatment of parkinsons disease in a mouse model
... interactions between the local degenerated cells and the transplanted stem cells 4.2 Stem cell transplantation Stem cells are defined by their capability for self-renewal and multipotent differentiation ... replacement as a possible strategy for the CNS is worth considering because stem cells could differentiate into neural cells Neural stem cells would be good candidate for cell replacement in neurodegenerative ... brother, sister-in-law and niece for their full and endless support for my study Finally, I am greatly indebted to my beloved husband, Dr Dong Yuan Hong for his encouragement and endless support...
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Characterization of adult human bone marrow mesenchymal stem cells for effective myocardial repair
... Stem cells 1.5.1.1 Embryonic stem cells 1.5.1.2 ES cell stem therapy is not ideal for clinical use 1.5.2 Adult stem cells 10 1.5.2.1 Bone marrow stem cells ... Eugene Sim, Philip Wong and Winston Shim Myocardial Matrix-driven Cardiac Differentiation and Integration of Human Mesenchymal Stem Cells [Manuscript submitted to Stem Cells and Development] Genevieve ... FIGURE EMBRYONIC STEM CELLS ARE TOTIPOTENT AND CAN DIFFERENTIATE INTO VARIOUS CELL TYPES FIGURE BONE MARROW STEM CELLS ARE MULTIPOTENT AND CAN DIFFERENTIATE INTO CELLS BELONGING TO...
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Human embryonic stem cells for genotoxicity testing
... exposure of hESC to reduced temperature (4oC and 25oC) for 24h and 48h Table Percentage of aberrant cells in untreated controls and in MMCtreated IMR90 and hESCs by PNA-FISH Page 32 42 viii SUMMARY ... physiology Lack of standardization is also a limitation with the use of primary explanted cells Human embryonic stem cells (hESCs) have been validated as a permanently stable and healthy human cell ... maintained at oC for 24h by PNA FISH hESCs maintained at 25 oC for 24h by PNA FISH 33 34 Figure hESCs maintained at oC for 48h by PNA FISH 34 Figure hESCs maintained at 25 oC for 48h by PNA FISH...
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