coli electroporation and phage propagation
PHOTOREACTIVATION OF ENTEROHEMORRHAGIC E. COLI, VRE AND P. AERUGINOSA FOLLOWING UV DISINFECTION
... than E coli (ATCC 11229) From the results of Kashimada et al (1996), the UV dose required for 90% inactivation of fecal coliforms, with and without photoreactivation, is computed to be 24 and 5.2 ... wastewater in a large-scale pilot plant and inactivation of selected bacteria in a laboratory UV device Biores Tech., 74, 141–150 Kamiko N and Ohgaki S (1989) RNA coliphage as a bioindicator of the ultraviolet ... without photoreactivation was 10.9 and 24.2 mW sec/cm2 , respectively The UV doses required for 90% inactivation of E coli (ATCC 11229) without photoreactivation was 2.5 and 7.0 mW sec/cm2 ,respectively...
- 6
- 317
- 0
Báo cáo khoa học: A strategy for the generation of specific human antibodies by directed evolution and phage display An example of a single-chain antibody fragment that neutralizes a major component of scorpion venom docx
... changes (Thr152Ile and Ser197Gly) occurred at frameworks and 3, respectively, and the third (Tyr164Phe), occurred at CDR1 (Table 2) Antibody 6009F was expressed in Escherichia coli and the presence ... (2005) 2591–2601 ª 2005 FEBS Clones 3F and C1 did not show the required affinity and ⁄ or functional stability to be neutralizing Directed evolution and phage display were used to improve these ... Kinetic rates and affinity constants of the soluble proteins corresponding to the scFvs 3F and C1 Kinetic rates and KD were calculated using BIA-EVALUATION v 3.2 software SE, standard error scFv...
- 11
- 679
- 0
animal cell electroporation and electrofusion protocols
... Pulse Length and Strength on Electroporation Efficiency Sek Wen Hui Introduction Electroporation is now a standard method of transfection and cell loading There is a variety of commercial electroporation ... size and/ or charge, fewer and fewer pores should participate, and this means that F,,, should decrease as the size and charge of “s” increase 21 Molecular Transport Owing to Reversible Electroporation ... by electroporation may also lead to cell death without irreversible electroporation itself having occurred According to our present understanding of electroporation itself, both reversible and...
- 358
- 334
- 0
plant cell electroporation and electrofusion protocols
... Pulse Length and Strength on Electroporation Efficiency Sek Wen Hui Introduction Electroporation is now a standard method of transfection and cell loading There is a variety of commercial electroporation ... size and/ or charge, fewer and fewer pores should participate, and this means that Fw,, should decrease as the size and charge of “s” increase 21 Molecular Transport Owing to Reversible Electroporation ... by electroporation may also lead to cell death without irreversible electroporation itself having occurred According to our present understanding of electroporation itself, both reversible and...
- 195
- 262
- 0
Electronics Technician Volume 7 Antennas and Wave Propagation
... characteristics and installation location, radiation patterns, and power and frequency-handling capabilities Be familiar with safety precautions for technicians working aloft; and discuss the ... digital data systems and includes discussions about SNAP II, laptop computers, and desktop computers Volume 7, Antennas and Wave Propagation, is an introduction to wave propagation, as it pertains ... Figure 2-4.—Standing waves of current and voltage on an antenna Figure 2-3.—Antenna and rf source This condition is called a STANDING WAVE of current The points of high current and high voltage...
- 124
- 305
- 0
Báo cáo hóa học: " Research Article A Study of Gas and Rain Propagation Effects at 48 GHz for HAP Scenarios" doc
... COST 255, “Radiowave Propagation Modelling for New Satellite Communication Services at Ku-Band and above,” Final Report, Brussels, Belgium, 2002 [20] K S Paulson, R J Watson, and I S Usman, “Diversity ... measured and simulated received signal levels of FabryPerot resonator filled with standard laboratory air at the resonant frequency of 48 GHz is shown in Figure Although both the measured and the ... Journal on Wireless Communications and Networking RAINFALL RADAR DATA Rain events can affect the propagation of electromagnetic waves in the millimeter wave band much more significantly than gas...
- 7
- 383
- 0
Báo cáo hóa học: " Research Article Survey of Channel and Radio Propagation Models for Wireless MIMO System" docx
... Transactions on Antennas and Propagation, vol 48, no 2, pp 137–146, 2000 C Bergljung and P Karlsson, Propagation characteristics for indoor broadband radio access networks in the GHz band,” in Proceedings ... the numbers sTx and sRx of scatterers at the Tx and Rx side, respectively; (ii) the steering vectors for all Tx and Rx scatterers, contained in the m × sTx and n × sRx matrices Φ and Ψ, respectively; ... single-bounce scattering between Tx and Rx Diffraction and multiple-bounced scattering are often neglected, however This implies a direct coupling of DoAs and DoDs and a joint Tx and Rx angular power spectrum...
- 19
- 472
- 0
Báo cáo y học: "Direct selection and phage display of a Gram-positive secretom" ppsx
... helper phage, transformed recombinant phagemids replicate from the plasmid origin of replication to form double-stranded DNA in the E coli host The resulting double-stranded recombinant phagemid ... lacIqZΔM15]) was utilized to construct the phagemid vector pDJ01 and phage display library E coli cells were incubated in yeast extract tryptone broth (2xYT) and E coli transformants in 2xYT with 20 ... with NsiI and EcoRI and ligated to the NsiI-EcoRI-cleaved vector pDJ01 This phagemid was named pSOF22 Production and functional assays of the SOF-displaying phagemid particles The phagemid particles...
- 15
- 297
- 0
Flexible engineering system design with multiple exogenous uncertainties and change propagation
... Systems Modeling and Analysis lab, for their friendship and help in the past several years Finally, I am grateful to my parents and my husband, for their constant encouragement and support throughout ... understand the nature of change and change propagation To date, the CPM approach has been applied into different domain However, most of the papers analyze interdependency and change propagation ... uncertainty and flexibility, flexible system design and change propagation management Several observations and research gaps have been drawn from the review First, the system concept generation and selection...
- 171
- 267
- 0
Proteomics approach on the identification of virulence factors of enteropathogenic and enterohemorrhagic escherichia coli (EPEC and EHEC) and further characterization of two effectors espb and nlei
... understand their mechanisms of infection and to develop the diagnostics and therapeutics for these pathogens Enteropathogenic Escherichia coli (EPEC) and enterohemorrhagic Escherichia coli (EHEC) ... attempts to investigate and understand the pathogenesis of EPEC and EHEC Approaches for understanding the pathogenesis of EPEC and EHEC, which involves studying the molecular and cellular basis of ... E coli that cause diarrhea diseases are now recognized: enterotoxigenic E coli (ETEC), enteroinvasive E coli (EIEC), enteroaggregative E coli (EAggEC), diffusely adherent E coli (DAEC), EHEC and...
- 180
- 399
- 0
Báo cáo khoa học: Selection of full-length IgGs by tandem display on filamentous phage particles and Escherichia coli fluorescence-activated cell sorting screening doc
... high-affinity and moderate-affinity IgG by tandem phage FACS Three fUSE5–ZZ–IgG phages displaying M18, YMF10 and VA IgGs were produced and purified as described above The M18, YMF10 and VA IgGs display ... IgG phagemid are then selected for antigen binding by standard phage biopanning We describe here the feasibility of propagating phage particles that stably display functional full-length IgGs, and ... experiments 1.5 0.5 Library 2294 Cycle Cycle Cycle Library selection by tandem phage biopanning and FACS To evaluate the utility of tandem phage panning– FACS for library screening, we used an anti-PA mouse...
- 13
- 310
- 0
Báo cáo khoa học: " The genome and proteome of a virulent Escherichia coli O157:H7 bacteriophage closely resembling Salmonella phage Felix O1" doc
... bacteriophage wV8 Bacteria E coli O157:H7 Other E coli Sensitive to bacteriophage wV8 Resistant to bacteriophage wV8 Reference strains of 12 common E coli O157:H7 phage types1 (1 strain /phage type) ... isolated phage V8 AMK assisted in the annotation and prepared the manuscript, EJL and SO propagated and purified the phage, and together with YMS contributed to the proteome analysis HWA and DMM ... at 100 kV and 60 kV, respectively Phage wV8 is a member of the Myoviridae and is morphologically identical to Felix O1 and related phages Viral particles were morphologically intact and generally...
- 5
- 221
- 0
antenna and propagation1 7801
... = Etd F E= 60 Pt Dt R = Etd Công thức cường độ điện trường điểm thu với đất bán dẫn (công thức Vanderpol) E = Etd F π R ε −1 x= ⇒ ' λ εp ' p πR x = λ ( ) (ε ) + ( 60λσ ) ε − + ( 60λσ d ) ' d '...
- 22
- 133
- 0
Tài liệu Báo cáo " synthesis, cloning and expression in escherichia coli of a gene coding for Mcoti-ii " ppt
... NdeI and XhoI restriction enzymes (fig 6) and ligated with pTYB12 vector had been digested by the same restriction enzymes, then transformated into E .coli BL21 (DE3) The resulting recombinant E .coli ... the 58 kD protein band was found in induced sample as the major band, acounted for 80% of total protein (lane 1,3,5 and 6) 58 kD Fig 10 SDS PAGE pattern of PI-17 proteins Mc Standard molecular ... Michal Lebl and Richard A Houghten (Editors), American Peptide Society, 2001, pp 387-388 Bozenna Rempola, T-Wilusz, W Markiewicz and M.Fkus, Synthesis, cloning and expression in E .coli of the...
- 9
- 497
- 0
Tài liệu Báo cáo khoa học: Basis of recognition between the NarJ chaperone and the N-terminus of the NarG subunit from Escherichia coli nitrate reductase pdf
... ± 0.2 and a binding constant (Kd) of 3.4 ± · 10)9 m and a second with a stoichiometry of 0.7 ± 0.1 and a Kd of 3.3 ± · 10)7 m (Fig 5A) Identical results were obtained using NarJ or NarJT and both ... References Magalon A & Mendel RR (2008) Biosynthesis and insertion of the molybdenum cofactor In EcoSal—Escherichia coli and Salmonella: cellular and molecular biology (Bock A, Curtiss R III, Kaper ... resonances and the appearance of new resonances upon complex formation indicated a slow exchange on the NMR timescale between the free and the bound forms for NarJT These results and the higher...
- 10
- 685
- 0
Tài liệu Báo cáo khoa học: Ionic strength and magnesium affect the specificity of Escherichia coli and human 8-oxoguanine-DNA glycosylases pdf
... strength and Mg2+ on the activity and specificity of OGG1, we measured the apparent values of k2 and k3 under conditions of low salt (KPi only) and no Mg2+, low salt and 20 mm Mg2+, and high salt ... and A by Fpg and OGG1 depends on buffer composition, ionic strength, Mg2+ concentration and several other factors 3748 Results Effects of ionic strength and divalent cations on the activity and ... THF:C ligand was 1.6-fold higher than for the THF:A ligand The presence of Mg2+ had little effect on the binding of Fpg to the THF:C ligand and slightly improved binding to the THF:A ligand, making...
- 14
- 567
- 0
Tài liệu Báo cáo khoa học: Mechanism of dihydroneopterin aldolase NMR, equilibrium and transient kinetic studies of the Staphylococcus aureus and Escherichia coli enzymes docx
... rate constants are similar between NP and MP and 2248 between HP and HPO in accordance with the similar shapes and sizes between NP and MP and between HP and HPO The different Kd values are proportional ... HP and 20 mM GA The reaction was initiated with 10 lM SaDHNA at 25 °C and quenched with M HCl The reverse reaction generated both DHNP and DHMP HP, DHNP, and DHMP were oxidized to HPO, NP, and ... using both DHNP and DHMP as the substrates and the reverse reaction (the formation of DHNP and DHMP) with HP and GA For the forward reaction, three concentrations each for DHNP and DHMP were used...
- 13
- 479
- 0
Tài liệu Báo cáo khoa học: Critical roles of Asp40 at the haem proximal side of haem-regulated phosphodiesterase from Escherichia coli in redox potential, auto-oxidation and catalytic control doc
... auto-oxidation rates and catalytic activities of Ec DOS Breakage of the salt bridge between Asp40 and Arg85, and the hydrogen bond network consisting of Asp40, two water molecules and His77, appear ... 2004 oxygen and carbon monoxide to a heme-regulated phosphodiesterase from Escherichia coli: kinetics and infrared spectra of the full-length wild-type enzyme, isolated PAS domain and Met95 mutants ... redox potential and auto-oxidation rates of Ec DOS PAS, and catalytic activities of full-length enzymes We found that the mutations at Asp40 markedly altered the redox potential and auto-oxidation...
- 6
- 423
- 0
Tài liệu Báo cáo khoa học: "Nonlinear Evidence Fusion and Propagation for Hyponymy Relation Mining" pdf
... Vesuvius and Kilimanjaro are volcanoes and Lhotse is similar to them, but Lhotse is not a volcano Therefore we should be very conservative and careful in hypernym propagation In our propagation ... evidence fusion and propagation significantly improve the precision and coverage of the extracted hyponymy data This is one of the technologies adopted in our semantic search and mining system ... with our evidence propagation method On the other hand, low quality labels get smaller score gains via propagation and are ranked lower Label propagation is performed in (Talukdar et al., 2008;...
- 10
- 378
- 0
Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản khảo sát chương trình đào tạo gắn với các giáo trình cụ thể tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct mở máy động cơ lồng sóc các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến tốc độ rôto n fi p2 sự cần thiết phải đầu tư xây dựng nhà máy phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25