coli and hek293t cells
Báo cáo sinh học: "Comparative expression profiling of E. coli and S. aureus inoculated primary mammary gland cells sampled from cows with different genetic predispositions for somatic cell score" ppt
... SCS-BTA18-Q and SCS-BTA18-q cells and respective control cells; the functional category, p-values and the number of genes are shown for E coli inoculated SCS-BTA18-Q and SCS-BTA18-q cells and S aureus ... inoculated cells and control cells at time points for each pathogen challenge and each of the inherited SCS-BTA18QTL alleles; A E coli inoculated cells; B S aureus inoculated cells; C E coli inoculated ... both S aureus and E coli inoculated cells, and E coli inoculated cells triggered a faster and more distinctive response to pathogen challenge than S aureus did To identify potential candidate genes...
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Tài liệu Báo cáo khoa học: Implication of the glutamine synthetase ⁄glutamate synthase pathway in conditioning the amino acid metabolism in bundle sheath and mesophyll cells of maize leaves doc
... little contamination from xylem, and vice versa Glx (glutamine and glutamate: ve carbon amide and amino acid) and Asx (asparagine and aspartate: four carbon amide and amino acid) were found to be ... Table Amino acid composition in leaves, and amino acid percentage ratio in the phloem exudates and leaves and xylem bleeding sap and leaves in the light and dark The amino acid composition in ... Arabidopsis, dark and sugar induce ASN1 and gln1-1, respectively, and repress gln1-1 and ASN1, respectively These expression patterns correlate with the relative abundance of asparagine and glutamine...
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Tài liệu Báo cáo khoa học: Ionic strength and magnesium affect the specificity of Escherichia coli and human 8-oxoguanine-DNA glycosylases pdf
... strength and Mg2+ on the activity and specificity of OGG1, we measured the apparent values of k2 and k3 under conditions of low salt (KPi only) and no Mg2+, low salt and 20 mm Mg2+, and high salt ... and A by Fpg and OGG1 depends on buffer composition, ionic strength, Mg2+ concentration and several other factors 3748 Results Effects of ionic strength and divalent cations on the activity and ... THF:C ligand was 1.6-fold higher than for the THF:A ligand The presence of Mg2+ had little effect on the binding of Fpg to the THF:C ligand and slightly improved binding to the THF:A ligand, making...
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Tài liệu Plant physiology - Chapter 3 Water and Plant Cells docx
... water Water is the most abundant and arguably the best solvent known As a solvent, it makes up the medium for the movement of molecules within and between cells and greatly influences the structure ... between water molecules and ions, and between water and polar solutes, in solution effectively decreases the electrostatic interaction between the charged substances and thereby increases their ... desert climates –4 Water and Plant Cells lation of solutes, closing of stomata, and inhibition of photosynthesis Water potential is one measure of how hydrated a plant is and thus provides a relative...
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Tài liệu Báo cáo khoa học: Multidentate pyridinones inhibit the metabolism of nontransferrin-bound iron by hepatocytes and hepatoma cells docx
... effects of DFP and novel tetradentate and hexadentate analogues on NTBI uptake and mobilization from rat hepatocytes and hepatoma cells DFO was included as a reference chelator Materials and methods ... and (26 ± 2) · 106 for hepatocytes and hepatoma cells, respectively Fig Kinetics of NTBI uptake from ferric citrate by (A) hepatocytes and (B) hepatoma cells Cells were incubated for 0–3 h with ... hepatocytes and Q7 hepatoma cells were marked and similar All chelators decreased uptake to 20% or less of the controls DFP and DFO almost completely abolished NTBI uptake at both 0.1 and mM chelator...
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Báo cáo khoa học: Enzymes of creatine biosynthesis, arginine and methionine metabolism in normal and malignant cells Soumen Bera1, Theo Wallimann2, Subhankar Ray1 and Manju Ray1 potx
... EAC and S180 cells, mice muscle, sarcoma tissue and the kidney of normal and sarcoma-bearing mice Mitochondria from EAC and S180 cells were prepared according to Moreadith and Fiskum [47] and ... presence of both AGAT and GAMT in EAC and S180 cells and in sarcoma tissue indicates that the upregulated proteins are entirely tumor-cell specific On the other hand, the influx and efflux rate of creatine ... normal and sarcoma-bearing mice, as well as from EAC and S180 cells In addition, ornithine and GAA were estimated also from the kidney of normal and sarcoma-bearing mice Enzyme assay AGAT and mitochondrial...
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Báo cáo khoa học: CpG and LPS can interfere negatively with prion clearance in macrophage and microglial cells pdf
... LPS-stimulated J774 and BMDM cells, was quite unexpected, and gives rise to the hypothesis that these cells are able to transiently convert RML-PrPSc It is worth noting that J774 and primary BMDM cells both ... and, in susceptible cell lines, the amount of PrP may dictate the rate of de novo synthesis of PrPSc To verify this in activated phagocytic cells, we stimulated J774 and BV-2 cells with LPS and ... 18 and 24 h following stimulation for BV-2 and J774 (antibody against PrP A7) and after and 12 h for BMDMs (antibody against PrP 12F10) The average of the mean fluorescence intensity is shown and...
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Hydrogen Energy and Fuel Cells docx
... deployment strategy for hydrogen and fuel cells – Implementing the transition to hydrogen and fuel cells – Funding the transition A European roadmap for hydrogen and fuel cells 16 17 17 18 19 19 20 ... ANNEX Hydrogen and fuel cell technologies and related challenges 25 ANNEX I High Level Group on Hydrogen and Fuel Cells Technologies 32 ANNEX II High Level Group on Hydrogen and Fuel Cells Technologies: ... resources, effort and tion Such measures should address both supply and demand, competencies to analyse and address non-technical and socio- taking into account global competitiveness, and reward tech-...
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eukaryotes and prokaryotes cells
... Prokaryotic Cells Eukaryotic cells small cells (< mm) larger cells (> 10 mm) always unicellular often multicellular no nucleus or any membranebound organelles always have nucleus and other membrane-bound ... Components cont • • • • • • • Lysosomes Cytoskeleton Centriole Cilium and Flagellum Microvilli Cell membrane Cell Wall Prokaryotic cells Diagram Components • • • • • • • • • • Cytoplasm Ribosomes ... Eukaryotic cells Diagram Components • • • • • • • • • Cytoplasm Nucleus Mitochondria Chloroplast Ribosomes...
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Prokaryotic and Eukaryotic Cells
... species of Bacillus and Clostridium Shapes and arrangements of bacteria There are six common shapes of bacteria: coccus, bacillus, coccobacillus, vibrio, spirochete, and spirullum and there are several ... asexual and sexual DNA circular linear Proteins assoc with DNA Basic Histone Plasma membrane No sterols Sterols Ribosomes 70S 80S Cytoskeleton Absent present PROKARYOTIC CELLS Key Functions of Cells ... peritrichous - flagella are randomly dispersed over the cell surface Axial filaments are modified flagella that occur in spirochetes Non-locomotor appendages Pili are longer and sparser than fimbriae...
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Báo cáo khoa học: Invasion of enteropathogenic Escherichia coli into host cells through epithelial tight junctions ppt
... h, and then immunostained for occludin and ZO-1 A chicken-wire pattern of distribution for occludin and ZO-1 was seen in uninfected control monolayers (Fig 2A,B) In uninfected cells, occludin and ... cell–cell interfaces, and the staining is continuous and uniform After infection with EPEC, the distribution of occludin and ZO-1 was significantly altered and breaks in occludin and ZO-1 staining ... the cell monolayer, and that EPEC invasion into host cells occurs through TJ membrane microdomains Cholesterol depletion is responsible for non-pathogenic E coli entry into the cells In our study,...
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Báo cáo khoa học: Theoretical study of lipid biosynthesis in wild-type Escherichia coli and in a protoplast-type L-form using elementary flux mode analysis potx
... Escherichia coli and Corynebacterium glutamicum Metab Eng 8, 353–369 20 Fischer E & Sauer U (2003) A novel metabolic cycle catalyzes glucose oxidation and anaplerosis in hungry Escherichia coli J ... bacterium Escherichia coli We elucidate the interaction between the subsystems involved in the synthesis of unsaturated and saturated fatty acids, phospholipids (including cardiolipin), and lipid A It ... membrane constituents and products may still be produced by such an L-form mutant, and investigated whether hitherto unknown bypass mechanisms exist This should prove useful for understanding such morphogenetic...
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Pulmonary tuberculosis associated with increased number and percentage of natural killer and B cells in the peripheral blood pot
... of CD3 + and CD4 + lymphocytes in the peripheral blood There was however no difference in the percentages of CD8 + and cells expressing HLA-DR molecules between the patients with PTB and normal ... to play a vital role in the control of MTB infection, while the role of other cells, such as CD8 + T -cells and γδ T cells, is still controversial (Flynn et al., 2000) It is primarily due to the ... on the role of NK cells in PTB NK cells have been shown to lyse MTB-infected monocytes and alveolar macrophages (Vankayalapati et al., 2005) This has been attributed to the NK cells promoting the...
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Báo cáo khoa học: Cholesterol and its anionic derivatives inhibit 5-lipoxygenase activation in polymorphonuclear leukocytes and MonoMac6 cells pot
... and 200 ng of prostaglandin B1 (PGB1) were added This mixture was prepared and analyzed by HPLC as further described Determination of 5-LO product formation in cells PMNL (10 · 106) and MM6 cells ... oxalacetic acid, and 10 lgÆmL)1 bovine insulin All cultures were seeded at a density of · 105 cells mL)1 MM6 cells were treated with ngÆmL)1 TGF-b1 and 50 nm vitamin D3 (VD3) for days Cells were harvested ... in PG and mm CaCl2 (PGC buffer) as indicated For incubations, cells were finally resuspended in PGC buffer The viability of cells was tested by trypan blue exclusion, and more than 95% of cells...
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Báo cáo khoa học: Differential regulation of fatty acid amide hydrolase promoter in human immune cells and neuronal cells by leptin and progesterone pdf
... leptin and progesterone on FAAH activity and expression (A) Effect of leptin on the activity of FAAH in human U937 and CHP100 cells and on the protein content and the mRNA of FAAH in U937 cells ... of AMT, NAT and PLD in the same cells (Figs 1A,B,D and data not shown) It should be recalled that also CHP100 cells are devoid of cannabinoid receptors [18] Both U937 and CHP100 cells have leptin ... dissociation constants (Kd) of 2.0 ± 0.1 and 2.2 ± 0.2 nM and maximum binding (Bmax) of 382 ± and 339 ± fmolÆmg protein)1, for U937 cells and CHP100 cells, respectively These values are in agreement...
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Tumor Microenvironment and Myelomonocytic Cells Edited by Subhra K. Biswas pptx
... (Gabrilovich and Nagaraj, 2009; Ostrand-Rosenberg and Sinha, 2009) 24 Tumor Microenvironment and Myelomonocytic Cells MDSC recruitment and expansion are regulated by several cytokines, chemokines and ... L and Karin, M (2009) IL-6 and Stat3 are required for survival of intestinal epithelial cells and development of colitisassociated cancer Cancer Cell, 15, 103-113 34 Tumor Microenvironment and ... the combination of immune complexes and TLR ligands, IL-10, TGF, and glucocorticoids (Mantovani et al 2004) In general terms, hallmarks of M2 and M2-like cells are a high expression of negative...
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Báo cáo khoa học: Nuclear import of mPER3 in Xenopus oocytes and HeLa cells requires complex formation with mPER1 pdf
... active in HeLa cells (A) Schematic representation of mPER proteins and derived fragments used for transient transfection into HeLa cells and their nucleocytoplasmic distribution (B) HeLa cells were ... murine PER and CRY proteins in Xenopus oocytes mPER1 and mPER2, but not mPER3, are phosphorylated and imported into the nucleus of Xenopus oocytes (A) 35S-labelled mPER1, mPER2, mPER3 and derived ... FEBS S Loop and T Pieler Nuclear import of circadian clock proteins A Fig Nuclear import of clock proteins in HeLa cells (A) The cells were transiently transfected with myc-tagged and flagtagged...
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Tumor Microenvironment and Myelomonocytic Cells Edited by Subhra K. Biswas docx
... (Gabrilovich and Nagaraj, 2009; Ostrand-Rosenberg and Sinha, 2009) 24 Tumor Microenvironment and Myelomonocytic Cells MDSC recruitment and expansion are regulated by several cytokines, chemokines and ... L and Karin, M (2009) IL-6 and Stat3 are required for survival of intestinal epithelial cells and development of colitisassociated cancer Cancer Cell, 15, 103-113 34 Tumor Microenvironment and ... the combination of immune complexes and TLR ligands, IL-10, TGF, and glucocorticoids (Mantovani et al 2004) In general terms, hallmarks of M2 and M2-like cells are a high expression of negative...
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Báo cáo khoa học: Are UV-induced nonculturable Escherichia coli K-12 cells alive or dead? ppt
... between untreated and UV-treated cells For untreated and both UV1- and UV2-treated cells, the relative activities of the EM and PP pathways in anaerobic conditions were about 91% and 9%, respectively ... hybridization of untreated cells (A), UV1-treated cells (B) and UV2-treated cells (C) before (A1, B1, C1) and after DVC method (A2, B2, C2) DVC method (incubation with nutrients and nalidixic acid) ... (EM) and pentose phosphate (PP) pathways of E coli K-12 cells Results are the mean of at least two independent experiments Anaerobic Aerobic EM Untreated cells UV1-treated cells UV2-treated cells...
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