codon optimization of the microdystrophin gene for duchenne muscular dystrophy gene therapy
Báo cáo Y học: Nuclear proteins that bind to metal response element a (MREa) in the Wilson disease gene promoter are Ku autoantigens and the Ku-80 subunit is necessary for basal transcription of the WD gene ppt
... response of the MT gene to heavy metals [10,12], the presence of MREs in the promoter of the WD gene suggests that MREs and their cognate binding proteins function in the regulation of WD gene expression ... the ends of the Ku-80 gene The EcoRI site was used to generate the IR, and the BamHI site was used to ligate the Ku-80 IR gene to pcDNA3.1(+) to yield pc80IR Expression of the Ku-80 IR gene was ... activity in HepG2 cells The relative positions of the MREs within the promoter region of the WD gene are indicated in Fig The four MREs are located in the proximal region of the WD gene promoter between...
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Báo cáo khoa học: Complex alternative splicing of the hKLK3 gene coding for the tumor marker PSA (prostate-specific-antigen) ppt
... [25,26] The first short sequence is similar to the consensus GGGGCUG of the intron splicing enhancer found downstream of the microexon of the chicken cardiac troponin T gene In this gene, the enhancer ... We looked for putative regulatory sequences because of the complexity of the splicing events affecting the middle of the hKLK3 gene Intron contains a high concentration of G triplets; these are ... splicing site at the beginning of the exon We obtained the 3¢ lacking part of this mRNA by 3¢ RACE-PCR As shown in Fig 3, the 3¢ end of this transcript (K3g) was identical to the 3¢ end of the major...
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Báo cáo Y học: Interaction of the GTS1 gene product with glyceraldehyde3-phosphate dehydrogenase 1 required for the maintenance of the metabolic oscillations of the yeast Saccharomyces cerevisiae pdf
... density at the beginning of continuous culture b The amplitude was estimated by subtracting the DO concentration (%) of the valley from that of the peak of the next wave c The number of experiments ... similar to the case of gts1D [16] The growth of tdh2D was disturbed, but the cell density reached 90% of that of the wild-type at the beginning of the continuous culture (Table 2) The culture ... metabolic oscillation Deletion of GTS1 resulted in the loss of the fluctuations in the synthesis of trehalose and cAMP [26], leading to the disappearance of the oscillations These results suggested that...
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Báo cáo khoa học: "Optimization of the Agar-gel Method for Isolation of Migrating Ascaris suum Larvae From the Liver and Lungs of Pigs" pptx
... the saline of some of the samples to a final concentration of 1% in the agar-gels and the incubation saline About 500 ml of crushed ice was added to some of the jars after the removal of the agar-gels ... al 1999), although there have been no systematic attempts to optimize the method or to evaluate the impact of the factors that may influence the recovery Therefore, the aim of the present study ... contents, they are seen to be extremely active, being able to swim around in the saline of the incubation jars (personal observation) Therefore, we tested the effect of lowering the temperature of the...
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Primer design for the PCR amplification of the coad gene
... result in the amplification of the original coaD gene The use of the Nco I restriction site dictates what the first nucleotide of the next triplet codon must be (G) In the coaD gene, however, the second ... (CG) BamH I restriction site (GGATCC) Complement of stop codon (CTA) Overlap with the strand complement to the 3'-end of the coaD gene Estimated Tm of 60°C [8 * (A+T) + 11 * (C+G)] GC content is ... of the original gene is described in Utilisation of the Nco I site 3'-end primer No C-terminal tag is being used, so we have to add a stop codon (TAG is the natural stop codon for PPAT) CG GGATCCCTA...
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Báo cáo y học: "Enhancement of the Click Chemistry for the Inverse Diels Alder Technology by Functionalization of Amide-Based Monomers"
... dimethyl-1,2,4,5-tetrazine-3,6-dicarboxylate The first highly active part of the construct, allows the ligation of e.g carrier molecules on the desired side of the molecule The second dienophile on the other side with lower ... of the dansyl derivative and the N-ethyl-diisoproylamine and the dansyl sulfamidoethylamine to the tetrazine product linked with 5-dansyl sulfamidoethylcarboxamide-2-yl For the syntheses of the ... 1665.9) for chemical reaction by the solid phase peptide synthesis (Figure 4) Ligation of the pentamer I with the tetrazine-dicarboxylate 6: One µmol of the pentamer I (Figure 3) and 5.5 µmol of the...
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EFFECT OF THE NUMBER OF THE VERTICAL PIPES FOR THE PASSIVE AERATION ON THE COMPOSTING RATE
... determine the effect of the number of the ventilation pipes on the composting rate, an attempt was made to correlate the composting rate with the number of the perforated pipes for air supply in the ... determine the real enthalpy of the composting reaction, ∆Η , due to a lack of data Therefore, the nutritional energy of the DF about 3500 kcal/kg-DF was assumed as the enthalpy of the reaction ... Temperature profiles The bed temperature profiles of each of the composting runs are shown in Fig At the beginning of the composting, after the raw materials were initially put into the reactor,...
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Thermoeconomic optimization of the steam power plant
... the input for another In the cost balance equations of each element, the necessary assumptions are received by the way of one missing from the unknown number So the input cost of another element ... conditions For instance, as the function is called as input in the pump, it should be on the right of the equation The unit c in equation is the exergy cost In the analysis as the input exergy cost of ... obtained thermoeconomically [8,9] In the thermoeconomic analysis of a system, the calculation is started from the point where power fluid enters the system Because the output of an element is the...
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Tài liệu Báo cáo khoa học: Homologous expression of the nrdF gene of Corynebacterium ammoniagenes strain ATCC 6872 generates a manganese-metallocofactor (R2F) and a stable tyrosyl radical (Y•) involved in ribonucleotide reduction ppt
... source of the native R2F-protein of the C ammoniagenes ribonucleotide reductase were transformants from the reintroduction of the nrdF gene into the strain of its origin description [4] after the ... that of other class RNRs or, instead, is a result of the interaction of the R2F with the inactivated (oxidized) form of the R1E We consider the first option unlikely Under these circumstances, the ... In the present study, we report data on homologous expression of the nrdF gene of C ammoniagenes strain ATCC 6872 This is the first report of the successful purification of high amounts of the...
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Tài liệu Informal Savings of the Poor : Prospects for Financial Inclusion doc
... the coverage of the formal financial system It is further observed that 58% of the labourers and 20% of salary earners prefer to keep their saving at home The survey has observed the saving of ... investment decisions According to the findings of the survey, for meeting unforeseen expenses, over 81% of the Landless form 40% of the total households From the above findings it is apparent ... harvesting Their income depends on the availability of work and also the income they receive From the income received, they have the tendency to save for future On account of seasonality, the savings...
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Tài liệu HIGHLIGHTS OF THE AUTHORISATION REQUIREMENTS FOR FUND DISTRIBUTION IN HONG KONG doc
... Document The Hong Kong Offering Document of a scheme should contain, inter alia, the following information: Constitution of the scheme (e.g the name, registered address, place and date of creation of ... apply [2] Section of the Handbook sets out the overarching principles and requirements that apply across different types of products governed by the Handbook, whilst Section of the Handbook (i.e ... distributed to the Hong Kong public, are subject to the authorisation of the Securities and Futures PwC February 2012 Regulatory Framework The major laws and regulations governing the authorisation of collective...
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Tài liệu Báo cáo khoa học: "Demonstration of the UAM CorpusTool for text and image annotation" docx
... Editing of the Tag Hierarchy Annotation Windows When the user clicks on the button for a given text file/layer, an annotation window opens (see Figure 3) This window shows the text in the top ... embedding of segments Annotated texts are stored using stand-off XML, one file per source text and layer See Figure for a sample The software does not currently input from or export to any of the various ... selection, and assign features to the selection Since stand-off annotation is used for both text and image, much of the code-base is common between the two applications The major differences are: i)...
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Tài liệu Book of Abstracts of the 60th Annual Meeting of the European Association for Animal Production docx
... Development of a genetic indicator of biodiversity for farm animals 119 Software tools for management of genetic variation 120 Demonstration of the opportunities offered by a software for inbreeding ... Secretary Prof Kostas Fegeros Rector of the Agricultural University of Athens and President of the Hellenic Society of Animal Production General Director of Animal Production, Dept Ministry of Rural ... Dept of Animal Sciences and Aquaculture of the Agricultural University of Athens Members Prof Stelios Deligeorgis Dept of Animal Sciences and Aquaculture, Agricultural University of Athens Prof...
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Tài liệu Báo cáo Y học: Regulation of transcription of the Dnmt1 gene by Sp1 and Sp3 zinc finger proteins doc
... Characterization of the promoter of the mouse Dnmt1 gene (A) Transcriptional activity of the mouse Dnmt1 promoter in NIH3T3 cells The top diagram shows the mouse Dnmt1 gene and the lower diagram shows the ... the Dnmt1 promoter Independent activation of the Dnmt1 promoter by Sp1 and Sp3 through the same GA motif Sp1 and Sp3 bound to the same cis-element in the promoter of the gene for Dnmt1 Therefore, ... clarify the distinct roles of Sp1 and Sp3 at different phases of the cell cycle The transcriptional cofactor p300 is coprecipitated in complexes with Sp1 [38] The activation of the promoter of the gene...
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Tài liệu Báo cáo Y học: Analyses of the CYP11B gene family in the guinea pig suggest the existence of a primordial CYP11B gene with aldosterone synthase activity docx
... and function of the hormonal system We first cloned the genes of the CYP11B family of the guinea pig The 11bhydroxylase of the guinea pig showed higher substrate specificity than the aldosterone ... (intron 6) for the remaining introns Finally, the 3¢ UTRs of the guinea pig CYP11B genes show only 42% similarity whereas the 3¢UTR of the guinea pig CYP11B2 gene shares up to 72% identity with the ... To answer the question how these genes might have evolved we determined the complete genomic structure of both genes As shown in Fig 6, both genes exhibit the typical organization of the family,...
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Đề tài " Stability and instability of the Cauchy horizon for the spherically symmetric Einstein-Maxwell-scalar field equations " doc
... Nevertheless, Formulation of predictability seems unnatural from the point of view of both physics and the theory of partial differential equations The blow-up of the curvature or even of the C ... equations The reader can recover the full result of the proposition from the estimates for ν in Section For the slighter weaker result then, by virtue of the co-area formula, it suffices to bound the ... in the literature, one for relatively high, the other for relatively low regularity, and discuss the status of each in the context of the initial value problem of this paper and in the light of...
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Báo cáo khoa học: Definition of the residues required for the interaction between glycine-extended gastrin and transferrin in vitro pptx
... of the wild-type value [18] The biochemical basis of the gastrin–transferrin interaction is still unknown Knowledge of the regions of transferrin required for the binding of gastrin, and of the ... (Fig 2A) Therefore, ferric ions must be present for formation of the complex between ApoTf and Ggly or Gamide The effect of ferric ions on the stability of the gastrin–ApoTf complex was then investigated ... important for both the formation and stability of the gastrin–ApoTf complex (A) Injection of the iron chelator ETDA (3 mM) into the BIAcore channel at the same time as ApoTf prevented the association...
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A position statement of the National Association for the Education of Young Children docx
... statement of the National Association for the Education of Young Children children, communicating with the child’s family, and evaluating the program’s effectiveness for the purpose of improving the ... construct their own understandings of the world around them Children contribute to their own development and learning as they strive to make meaning out of their daily experiences in the home, the ... planning C The methods of assessment are appropriate to the age and experiences of young children Therefore, assessment of young children relies heavily on the results of observations of children’s...
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So, you want to go to grad school in Economics? A practical guide of the first years (for outsiders) from insiders pptx
... in terms of the general, and touch abstract and concrete in the same flight of thought He must study the present in the light of the past for the purposes of the future No part of man’s nature ... the first year Besides the professors, the TA’s of the courses you are taking in your first year are also an important part of the first year life Don’t hesitate to talk to them Go to their office ... take care of these requirements before entering grad school! Life in the first year The first year is often considered the breaking point of your Ph.D career It is the most demanding part of it,...
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Tìm thêm: xác định các mục tiêu của chương trình khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam nội dung cụ thể cho từng kĩ năng ở từng cấp độ phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc các đặc tính của động cơ điện không đồng bộ đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng 9 tr 25