cloning expression and purification of a fragment of the p murina msg
Báo cáo y học: " Pneumocystis murina colonization in immunocompetent surfactant protein A deficient mice following environmental exposure" pdf
... Carlsbad, CA) and total RNA was isolated The RNA was treated with RNAase free-DNAase and recovered by phenol:chloroform extraction and ethanol precipitation The RNA was evaluated in a spectrophotometer ... preparation of the manuscript ADA carried out the animal studies and ELISA JAD carried out the molecular studies PDW provide overall leadership to the design of the experiments, data analysis, and ... enhanced attachment to mouse alveolar macrophages [24] SP -A also displays other protective activities in the host response Binding of SP -A to gram-negative bacteria, Mycoplasma pneumoniae, and...
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Báo cáo y học: "Inflammatory responses to acute pneumovirus infection in neonatal mice" pps
... collated data and assembled first draft of the manuscript All authors read an approved the final draft Authors’ Information Dr Joseph B Domachowske is a Professor of Pediatrics, Microbiology, and ... 7:320 http://www.virologyj.com/content/7/1/320 Page of 14 Dakhama A, Park JW, Taube C, Joetham A, Balhorn A, Miyahara N, Takeda K, Gelfand EW: The enhancement or prevention of airway hyperresponsiveness ... quantitative RT-PCR assay targeting the PVM small hydrophobic (SH) gene as previously described [31], and expressed as copies PVM SH gene per copies cellular GAPDH (PVM SH /10 GAPDH) Preparation of single...
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A non mouse adapted dengue virus strain as a new model of severe dengue infection in AG129 mice
... serum of engrafted mice early post-infection and at later time-points The animals developed paralysis, thrombocytopenia and a prolonged partial thromboplastin time characteristic of DHF (An et al., ... the extent of tissue damage and vii Summary vascular leakage was minimal; basal production of these cytokines was instead measured in the animals at moribund state, where tissue damage and vascular ... various DEN vaccines have reached advanced developmental stages, the progress for validation of other potential vaccine candidates is largely hampered by the lack of a suitable DEN challenge animal...
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báo cáo hóa học: " Astrogliosis is delayed in type 1 interleukin-1 receptor-null mice following a penetrating brain injury" pptx
... of two glutamate transporters, glutamate aspartate transporter (GLAST) and glutamate transporter-1 (GLT-1/EAAT2), the glutamate The induced levels of the protease-activated receptor, PAR-1, were ... (Seattle, WA) All mice were bred and maintained at the Hershey Medical Center by the Department of Comparative Medicine, an AAALAC accredited facility Animal experimentation was in accordance ... Western and ELISA JKK and SWL designed and supervised the studies All authors have read and approved of the final manuscript 15 16 17 Acknowledgements This work was supported by a grant from the National...
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báo cáo hóa học: " Interleukin-1 receptor 1 knockout has no effect on amyloid deposition in Tg2576 mice and does not alter efficacy following Aβ immunotherapy" pot
... RWP performed the A ELISA VMH performed the image quantification and immunohistology YL performed A plasma ELISA and aided in the preparation of the manuscript PC performed Iba1 immunostaining ... induction of experimental autoimmune encephalitis (EAE) and nasal vaccination with glatiramer acetate reportedly decrease amyloid plaques in APP transgenic mice [48] Another report by the same group ... MN, Morgan D: Passive immunotherapy against Abeta in aged APP-transgenic mice reverses cognitive deficits and depletes parenchymal amyloid deposits in spite of increased vascular amyloid and microhemorrhage...
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báo cáo hóa học: " Treatment with gelsolin reduces brain inflammation and apoptotic signaling in mice following thermal injury" potx
... supervised and edited the manuscript YMY participated in the design of the experiments, funding of the projects, and preparation of the manuscript All authors have read and approved the final ... QHZ participated in the design of the study; personally conducted a significant portion of the experiments presented in the manuscript, and participated in the writing of the manuscript QC participated ... ameliorating cerebral inflammatory lesions and apoptosis via acceleration of recruitment of monocytes and down-regulation of phospho-ERK1/2 expression, and also via improvement of peripheral T...
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Báo cáo hóa học: " Immune response during acute Chandipura viral infection in experimentally infected susceptible mice" potx
... 96-well flat bottom culture plate Mitogens Concanavalin A (Con A) and lipopolysaccaride (LPS)(Sigma) derived from E coli and BPL inactivated Chandipura virus antigen were added 0.1 μg, 0.01 μg and ... Chadha MS, Arankalle VA, Jadi RS, Joshi MV, Thakare JP, Mahadev PVM, Mishra AC: An outbreak of Chandipura virus encephalitis in the eastern districts of Gujarat state, India Am J Trop Med Hyg 2005, ... fatality rate in children in Andrapradesh, India, in associated with Chandipura virus Lancet 2003, 364:869-874 Mosmann T: Rapid colorimetric assay for cellular growth and survival: application of proliferation...
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Báo cáo khoa học: "Effects of cyclosporin A treatment on the pathogenesis of avian leukosis virus subgroup J infection in broiler chickens with Marek’s disease virus exposure" ppt
... Molecular Biochemicals, Indianapolis, IN) The primers used have been described [37] and produced an amplicon of approximately 545 bp Amplification and detection of specific products was undertaken ... Schat, K A and Lucio B Relationship between the immunosuppressive potential and the pathotype of Marek's disease virus isolates Avian Dis 1998, 42, 124-132 Campbell, W F and Frankel, J W Enhanced ... had neutralizing antibody at the end of the experiment The percentage and titers of the neutralizing antibody positive samples in the Oil group was similar to those given CSP Histopathology All...
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Báo cáo khoa học: "The effects of cyclophosphamide treatment on the pathogenesis of subgroup J avian leukosis virus (ALV-J) infection in broiler chickens with Marek''''s disease virus exposure" potx
... reticuloendotheliosis In: D E Swayne et al (eds) A laboratory manual for the isolation and identification of avian pathogens 4th ed pp 185-196 The American Association of Avian Pathologists 1998 11 Fadly ... Current status of diagnosis, epidemiology and control of ALV-J In: Proceedings of the avain tumour viruses symposium, pp 5862 Reno, Nevada: American Association of Avian Pathologists 1997 23 Payne ... Cincinnati, OH) Each RNA sample was resuspended in 20 µl of diethyl pyrocarbonate (DEPC) treated water and stored at −80oC until use ALV-J and cyclophosphamide Real time RT-PCR RT-PCR was performed...
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Báo cáo khoa học: "Mucosal mast cell-derived chondroitin sulphate levels in and worm expulsion from FcRγ-knockout mice following oral challenge with Strongyloides venezuelensis" doc
... experiment they were supplied with feed and water ad libitum Parasite The strain of S venezuelensis originally isolated from a brown rat in Okinawa Prefecture, Japan [6] and established as a laboratory ... Ishiwata K, Uchiyama F, Maruyama H, Kobayashi T, Kurokawa M, Nawa Y Glycoconjugates and host-parasite relationship in the mucosal defense against intestinal nematodes In: Ogra PL, Mestecky J, Lamm ... sulphates and mouse mast cell protease1 (mMCP-1) in gut washings were assayed on day and post challenge Chondroitin sulphates in gut homogenate were assayed on day post challenge EPG and adult...
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Báo cáo y học: " PCEP enhances IgA mucosal immune responses in mice following different immunization routes with influenza virus antigens" pdf
... and coordination of all of the mouse trials and assays Additionally, NFE and GKM contributed to the primary preparation of this manuscript VG and LAB were involved with critical analysis of the ... Foundation and the Bill and Melinda Gates Foundation through the Grand Challenges in Global Health Initiative The assistance from Barry Carroll and Animal Care services at VIDO was greatly appreciated ... of Laboratory Animals as indicated by the Canadian Council on Animal Care and was approved by the Animal Care Committee of the University of Saskatchewan BALB/c mice were obtained from Charles...
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Báo cáo y học: "Early Reversal of experimental colitis disease activity in mice following administration of an adenoviral IL-10 vector" pps
... (MHJ) and (TA) participated in visual sample processing and analysis Authors (PJ) and (YW) helped conceive and design animal studies Author (TJ), (YW) and (JSA) conceived and designed the study Acknowledgements ... disease therapy Aliment Pharmacol Ther 2001, 15(8):1211-8 Dieleman LA, Pena AS, Meuwissen SG, van Rees EP: Role of animal models for the pathogenesis and treatment of inflammatory bowel disease Scand ... significance of increased appearance of MAdCAM-1 in IBD is supported by several reports which show that immunoneutralization of either MAdCAM-1 or its ligand, the α4β7 integrin, attenuate inflammation...
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Báo cáo y học: " Environmental exposures and their genetic or environmental contribution to depression and fatigue: a twin study in Sri Lanka" potx
... in the same household as one another These twins will necessarily share aspects of their ‘standard of living’ rating (structural materials, and toilet and water facilities of the abode) A payment ... to the interview) A substantial percentage of the participants refused the payment and instead requested it to be donated back to the research project [18] Analysis A database was constructed and ... undertook the statistical analyses and wrote the first draft MH and AS were principal investigators, responsible for study’s design and implementation MH and PMcG supervised the statistical analyses and...
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Báo cáo y học: "A VLP-based vaccine targeting domain III of the West Nile virus E protein protects from lethal infection in mice" pptx
... DIII-based approaches and its anticipated good safety profile make it an attractive candidate for WNV prophylaxis both in humans and in veterinary applications Methods Expression and Purification of ... Production of the AP205 VLP Carrier The bacteriophage AP205 has first been isolated from Acinetobacter spp and belongs to the Leviviridae family, a group of ssRNA phages which infects a wide range of ... in depletion of bacterial LPS Expression and Purification of Recombinant Domain III of the E Glycoprotein of WNV A DNA fragment encoding domain III of the glycoprotein E of WNV NY99 was amplified...
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Báo cáo y học: " Pulmonary fibrosis induced by H5N1 viral infection in mice" doc
... survived the acute stage but developed late stage disease acceleration as assessed by the presence of inactivity, weight loss, inappetence and emaciation Macroscopical and histopathological evaluations ... disease progression at late stage, as assessed by the presence of inactivity, weight loss, inappetence and emaciation Macroscopical and histopathological evaluations showed that these mice presented ... case of avian influenza A (H5N1) in Mainland China Lancet 2006, 367:84 Bay A, Etlik O, Oner AF, Unal O, Arslan H, Bora A, Davran R, Yuca SA, Dogan M: Radiological and clinical course of pneumonia...
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Báo cáo y học: " Cigarette smoke worsens lung inflammation and impairs resolution of influenza infection in mice" ppt
... experiments GPA was involved in design and interpretation of experiments, histological analysis and writing the paper All authors read and approved the final manuscript Acknowledgements We thank ... BALFs of the mice in each group were pooled for all of these assays For the assays in panels g and h, this graph is representative of two assays run with the same samples, and every sample was run ... twice in and resuspended in FACS buffer and paraformaldehyde (PFA) was added to a final concentration of 1% Data were acquired on a BD FACSCalibur, gated on lymphocytes by standard forward and side...
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Báo cáo y học: " Hyperresponsiveness to inhaled but not intravenous methacholine during acute respiratory syncytial virus infection in mice" pot
... 191:387-395 Dakhama A, Park JW, Taube C, El Gazzar M, Kodama T, Miyahara N, Takeda K, Kanehiro A, Balhorn A, Joetham A, Loader JE, Larsen GL, Gelfand EW: Alteration of airway neuropeptide expression and ... ASM, and implicates the integrity of the epithelial layer as important in the response to inhaled agonists Epithelial mechanisms are the most likely candidates to explain the results of the present ... KJ, Gao Y, Vanhoutte PM: Epithelial modulation of airway smooth muscle American Journal of Physiology 1990, 258:L254-262 Mathe AA, Hedqvist P: Effect of prostaglandins F2 alpha and E2 on airway...
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Báo cáo y học: "Childhood physical abnormalities following paternal exposure to sulfur mustard gas in Iran: a case-control study" pot
... ophthalmologist that based their decision on hospital documents, mustard gas exposure stigmata, and clinical signs and symptoms of chronic complications of exposure, and appropriate paraclinical studies as ... database and by placing signposts and billboards across the city Control population Rabat is a nearby city that has ethnic, cultural, and geographical characteristics similar to Sardasht but was ... group, age, occupation, educational level, date of marriage; close relative marriage, and any history of chemical injury The same data was also gathered from the non-exposed (Rabat) group Trained...
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Báo cáo sinh học: "Tissue specific promoters improve specificity of AAV9 mediated transgene expression following intra-vascular gene delivery in neonatal mice" pptx
... designed and cloned the plasmids and harvested tissues BDT performed the RNA transcript analysis and helped to draft the manuscript CSM participated in the design of the study and helped to draft the ... isoforms of MLC-2 are typically expressed in the atria and ventricles of the mammalian heart during embryonic development and maturation, and later in the adult heart [21] The ratderived MLC promoter ... non-phosphorylatable and MLC-2, phosphorylatable Ca2+ dependent phosphorylation of MLC-2 by MLC kinase provides an important a regulatory function in the contraction of cardiac, skeletal and...
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Discovery of host lipid biomarkers for tuberculosis infection in mice
... applied to microarray data in attempts to diagnose cancer Several issues are of great importance when applying any machine learning tool Especially data sets with many more features than samples ... either side of the hyperplane define the maximum margin hyperplanes Adopted from Bellazzi and Zupan (Bellazzi and Zupan, 2008) 2.3.4.2.2 SVM Parameter Optimization Cost (C) is the only parameter for ... specified the total number of carbon atoms and double bonds in the fatty acids chains of a given lipid Theoretical transitions for a lipid of a given class and with a specific number of carbon atoms and...
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