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Tài liệu Báo cáo khoa học: Characterization of electrogenic bromosulfophthalein transport in carnation petal microsomes and its inhibition by antibodies against bilitranslocase docx
Ngày tải lên :
20/02/2014, 01:20
... from either a
perfect shield of site B afforded by bilirubin, or, other-
wise, by an alternative conformation of the bilirubin–
bilitranslocase complex, totally missed by antibody B.
Cyanidin 3-glucoside ... was not stimulated by ATP
(data not shown).
Immunoblots of carnation petal membrane
fractions
Membrane proteins from subcellular fractions of carna-
tion petals were separated by SDS ⁄ PAGE and ... – University of Trieste) for the immunization
and bleeding of rabbits; to Silvia Zezlina for the affin-
ity purification of antibody A from rabbit sera; to Dr
Paolo Ermacora and Prof Giorgio Honsell...
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Báo cáo khoa học: Characterization of HbpR binding by site-directed mutagenesis of its DNA-binding site and by deletion of the effector domain pot
Ngày tải lên :
07/03/2014, 17:20
... purification of HbpR itself and has been
shown previously not to disturb HbpR-mediated tran-
scription activation [19]. SDS ⁄ PAGE of the soluble cell
extracts of E. coli BL21 (pHB171) showed a protein of
43 ... harvested by centrifu-
gation (5 min, 1575 g, at room temperature) and resuspended
in Mops medium [5.5 gặL
)1
of Mops free acid, 5.1 gặL
)1
of
Mops sodium salt, 0.5 g ặ L
)1
of NaCl, 1 gặL
)1
of NH
4
Cl,
0.06 ... activa-
tion of HbpR must proceed differently from that of
XylR, a fusion protein between DA-HbpR and the
A-domain of XylR was found to be incapable of acti-
vating the hbpC promoter (not shown). Hence,...
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Báo cáo Y học: Complexation of ytterbium to human transferrin and its uptake by K562 cells pot
Ngày tải lên :
08/03/2014, 09:20
... filter.
RESULTS
Binding of Yb
3+
to apo-hTf
The thermodynamics of binding of Yb
3+
to apoTf.
Binding of Yb
3+
to apo-Tf was investigated by addition
of aliquots of Yb
3+
to a solution of apo-TF in 100 ... concentration of Yb-
Tf (Fig. 7C). The
FF decreased to half of its original value
when 18 mol equiv of Yb-Tf was incubated with cells in the
presence of FITC-Fe
2
-Tf. Therefore part of the FITC-Fe
2
-
Tf ... NMR spectrum of apo-Tf in the presence of 10 m
M
bicarbonate was recorded after addition of Yb
3+
(or Lu
3+
)
Fig. 2. Kinetics of binding of Yb
3+
to apo-Tf. The final concentration of
the protein...
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Báo cáo khoa học: Photoregulation of DNA transcription by using photoresponsive T7 promoters and clarification of its mechanism doc
Ngày tải lên :
15/03/2014, 10:20
... instead of Azo (Fig. 1). Clear-cut on–off
photoregulation of DNA transcription was obtained
because of the efficient suppression of T7 RNA
polymerase (RNAP) binding to the DM-azo in the
trans form. By ... stability of the cis form between
Azo and DM-azo. (A) Illustration of the reversible photoisomeriza-
tion of Azo and thermal isomerization of cis-Azo. (B) Quantitative
calculation of cis-form ... Photoregulation of DNA transcription by using
photoresponsive T7 promoters and clarification of its
mechanism
Xingguo Liang
1
, Ryuji Wakuda
1
, Kenta Fujioka
1
and Hiroyuki Asanuma
1,2
1 Department of Molecular...
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Báo cáo khoa học: Transcriptional regulation of the desferrioxamine gene cluster of Streptomyces coelicolor is mediated by binding of DmdR1 to an iron box in the promoter of the desA gene doc
Ngày tải lên :
16/03/2014, 11:20
... sequence is
located inside the iron box of desA (nucleotides 7–12
of the iron box) , explaining the regulation of desA
expression by binding of DmdR1 to the iron box. The
same overlapping was found ... the decarboxylation of lysine by a lysine
decarboxylase encoded by the desA gene [8,9]. Lysine
decarboxylases occur in different Streptomyces species
and are involved in the utilization of l-lysine ... Production of desferrioxamine was restored
by complementation with the whole wild-type desABCD cluster, but not by
desA alone, because of a polar effect of the desA gene replacement on
expression of...
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Báo cáo khoa học: Molecular characterization of gonad-inhibiting hormone of Penaeus monodon and elucidation of its inhibitory role in vitellogenin expression by RNA interference pptx
Ngày tải lên :
23/03/2014, 07:20
... study of Pem-GIH in
the shrimp.
Biological assay for vitellogenesis-inhibiting
activity of Pem-GIH by dsRNA-mediated
functional knockdown
To test whether the knockdown of Pem-GIH expres-
sion by ... ratio of Vg to actin transcripts in the GIH-depleted
background suggested that functional knockdown of
Pem-GIH led to the induced expression of Vg in the
ovary.
Discussion
Because of the lack of ... the
5Â-region of Pem-GIH. The negative PCR is indicated by ) ve. The actin transcript of P. monodon was used as an internal control of the
amount of RNA template. Each panel shows tissue distribution of...
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Báo cáo khoa học: Characterization of testis-specific serine–threonine kinase 3 and its activation by phosphoinositide-dependent kinase-1-dependent signalling doc
Ngày tải lên :
23/03/2014, 11:20
... Characterization and regulation of TSSK3
TSSK3 activation is different from its involvement in
the activation of PKB. Inhibitors of other protein kin-
ases, known to be activated by insulin (like p70S6K,
p38, ... the release of radioactivity during
the fifth cycle (data not shown). Substitution of Val to
Tyr in peptide 2 reconstituted phosphorylation of this
peptide by TSSK3 almost to level of peptide 4 ... insulin
treatment of cells may also slightly (twofold) enhance
PDK1 kinase activity [33]. Therefore, it is thought that
the role of PDK1 in the activation of other kinases is
governed by its cellular...
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Báo cáo khoa học: "Unsupervised Discovery of Generic Relationships Using Pattern Clusters and its Evaluation by Automatically Generated SAT Analogy Questions" pot
Ngày tải lên :
23/03/2014, 17:20
... resource itself and how to evaluate its intrinsic
quality. In (Davidov and Rappoport, 2008) we show
how to use the resource for a known task of a to-
tally different nature, classification of relationships
between ... in related work.
6.1 Discovery of Known Relationships
To estimate recall of our pattern cluster set, we
attempted to estimate whether (at least) a subset
of known relationships have corresponding ... clustering methods that as-
sign given sets of pairs into several clusters, where
each cluster corresponds to one of a known set of re-
lationship types. Their classification setting is thus
very different...
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Báo cáo khoa học: Binding of activated Factor XII to endothelial cells affects its inactivation by the C1-esterase inhibitor pptx
Ngày tải lên :
23/03/2014, 20:22
... attack
by kallikrein generated by activation of PPK as a
consequense of vessel injury. The activation of FXII
and PPK/HK may start with a local increase in the
concentration of Zn
2+
as a result of ... although the activity of
FXIIageneratedonthecellsbyactivationofFXIIinthe
presence of Zn
2+
was significantly higher than that
generated in the absence of Zn
2+
, the fraction of bound
FXII that became ... activation of FXII and thereby the entire
system [6]. In blood plasma the proteolytic efficiency of the
proteinases is attenuated by endogenous inactivation of
FXIIa and kallikrein by serine proteinase...
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Báo cáo Y học: Expression of uncoupling protein-3 in subsarcolemmal and intermyofibrillar mitochondria of various mouse muscle types and its modulation by fasting docx
Ngày tải lên :
24/03/2014, 04:21
... degree of accuracy, but only if
obtained on the same gel.
The density of UCP3 protein (i.e. the intensity of the
signal expressed per mg of proteins) was similar in the SS
mitochondria of the ... the
relative amounts of UCP3 protein and mRNA in the three
muscles are comparable.
Effect of fasting on UCP3 protein expression
We and others have shown that fasting induces upregula-
tion of UCP3 mRNA ... consisted of 2 min of denatur-
ing at 95 °C, followed by 30 cycles with 95 °Cdenaturing
for 1 s, 56 °C annealing for 5 s and 72 °C extension for
16 s. The fluorescent product was detected at the end of...
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Báo cáo khoa học: Binding of the viral immunogenic octapeptide VSV8 to native glucose-regulated protein Grp94 (gp96) and its inhibition by the physiological ligands ATP and Ca2+ Ming Ying and Torgeir Flatmark pot
Ngày tải lên :
30/03/2014, 11:20
... increase in its
hydrodynamic radius, upon binding of Ca
2+
[30].
Peptide-binding properties of native rGrp94 and
its inhibition by physiological ER ligands
Since the original studies by Srivastava ... lM of VSV8 and a global
R
max
(obs) of 27 RUặ(ng proteinặmm
)2
). (C) D ouble-reciprocal plot of
the equilibrium-binding isotherms obtained in the absence of
ATP ⁄ Ca
2+
(d), in the presence of ... properties of native Grp94, inclu-
ding its binding parameters. However, interactions of
Grp94 with its two related ER lumenal chaperones
Grp78 ⁄ BiP and calreticulin were excluded from this
study. By...
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Báo cáo khoa học: Regulation of phospholipid biosynthesis by phosphatidylinositol transfer protein Sec14p and its homologues pot
Ngày tải lên :
30/03/2014, 15:20
... condition
of high PtdCho turnover, the addition of inositol can
consume part of the PA pool, permitting that partial
repression of INO1 transc ription. Alternatively, the a ddi-
tion of inositol ... the inability of Sfh2p to
decrease PtdCho turnover to wild-type levels i s responsible
for the inab ility of S fh2p to complement the INO1
regulatory defect caused by the absence of Sec14p, we
measured ... by the following lines of evidence (Fig. 1), namely that
(a) mutations in genes encoding structural enzymes of the
cytidine diphosphate choline (CDP-choline) pathway for
PtdCho biosynthesis bypass...
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Báo cáo khoa học: Regulation of ascidian Rel by its alternative splice variant pdf
Ngày tải lên :
30/03/2014, 20:20
... repre-
sentation of the structures of the As-rel genome and mRNAs of
As-rel1 and As-rel2. (B) Genome sequence of splicing sites. Note that
As-rel1 mRNA is generated by splicing at the sites shown by open
arrowheads, ... amounts of transfected DNA were kept constant
(1.5 lg) by adding an empty vector (pBIND or pCI-neo-Flag vector).
The level of activity was normalized on the basis of the level of activity
of control ... was verified
by Western blotting of the fusion protein.
Preparation of the genome and genomic PCR
The genome of the ascidian, H. roretzi, was prepared from
muscles. A 5 mL volume of extraction...
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Báo cáo khoa học: In vivo cross-linking of nucleosomal histones catalyzed by nuclear transglutaminase in starfish sperm and its induction by egg jelly triggering the acrosome reaction pdf
Ngày tải lên :
31/03/2014, 07:20
... panel)
are shown by bold lines. The concentration of acetonitrile is shown by
a dotted line. Arrows indicate the position of substance X.
Fig. 7. Sucrose density-gradient analysis of nucleosome ... transglutaminase.
Determination of the DCA-binding sites of histone H2B
Because the carboxamide group of Gln9 of histone H2B is
selectively transamidated with the e-amino group of Lys5 or
Lys12 of histone H4 in ... analyzed by MALDI-TOF-MS, and was
found to have a relative molecular mass (M
r
) of 650.32. This
M
r
value is 17 units less than the sum of the M
r
of DCA
(335.50) and that of a tripeptide, Gly–Gln–Lys...
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Báo cáo hóa học: " Identification of a contemporary human parechovirus type 1 by VIDISCA and characterisation of its full genome" pdf
Ngày tải lên :
20/06/2014, 01:20
... the root of
contemporary strains, suggesting that VP0 may have been
acquired by a more recent ancestor of the group by recom-
bination.
Discussion
Enteritis is caused by a spectrum of viruses ... of the first stage
PCR product, under a touchdown cycling protocol: 95°C,
4 min, followed by 10 cycles of 94°C/30s, 65°C, 30s
(temperature decrease of 1°C per cycle), 72°C 1 min, fol-
lowed by ... Central
Page 1 of 10
(page number not for citation purposes)
Virology Journal
Open Access
Research
Identification of a contemporary human parechovirus type 1 by
VIDISCA and characterisation of its full...
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