cisplatin arsenic and hyperthermia on dna repair protein expression
Báo cáo khoa học: Solution structure and backbone dynamics of the XPC-binding domain of the human DNA repair protein hHR23B docx
... aspects of proton-carboncarbon-proton three-dimensional correlation spectroscopy of 13C-labeled proteins J Magn Reson 87, 620– 627 Farrow NA, Zhang O, Forman-Kay JD & Kay LE (1994) A heteronuclear ... residues conserved among the STI1 homologs are marked in blue, and the Pro residues not conserved among the STI1 homologs are marked in cyan (B) Ribbon presentation of the XPCB–hHR23B three-dimensional ... excision repair J Biol Chem 276, 18665–18672 Wittekind MML (1993) HNCACB, a high-sensitivity 3D NMR experiment to correlate amide-proton and nitrogen resonances with the alpha- and beta-carbon resonances...
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ELUCIDATING THE ROLE OF REDOX EFFECTS AND THE KU80 C-TERMINAL REGION IN THE REGULATION OF THE HUMAN DNA REPAIR PROTEIN KU
... AND THE KU80 CTERMINAL REGION IN THE REGULATION OF THE HUMAN DNA REPAIR PROTEIN KU DNA double strand breaks (DSB) are among the most lethal forms of DNA damage and can occur as a result of ionizing ... modifications wtKu and DNA- PK were analyzed for Ku80CTR interaction under native and PICUP conditions with or without the addition of DNA or ATP g wtKu were subjected to PICUP crosslinking conditions ... used and Ku protein preparations were titrated into the binding reactions Wild type and Ku70/80C under reduced conditions displayed identical binding and, upon oxidation with diamide, minimal DNA...
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Báo cáo hóa học: "DNA polymeraseh protein expression predicts treatment response and survival of metastatic gastric adenocarcinoma patients treated with oxaliplatin-based chemotherapy" pot
... plus cisplatin Spearman correlation analysis was further done to confirm the correlation between polh expression and clinicopathologic features Pearson contingency coefficient shown that polhexpression ... POLh protein was detectable in the nucleus of gastric cancer cell (× 400) Table DNA polymerase h protein expression and clinical treatment response Table Correlation between POL h expression and ... cases (23 out of 80), and the expression was modest However, strong correlation was found between polh expression and treatment response as well as survival All the results demonstrated that polh...
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Báo cáo khoa học: " Modeling effect of the septic condition and trauma on C-reactive protein levels in children with sepsis: a retrospective study" pdf
... actually model A) and DG, and based on these analyses we could draw the same conclusions concerning SEP and DG as we already had done Since the analysis was intended as exploratory, we consider the ... and parameter estimates are presented in Table We present two unconditional and two final models Because the SEP and DG predictors take on three and two discrete values, respectively, equations ... each septic category and over time The magnitude of this additional elevation in surgical patients therefore does not depend on septic condition Computing the model equations (Table 6), we can...
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THE INFLUENCE OF THE KU80 CARBOXY-TERMINUS ON ACTIVATION OF THE DNA-DEPENDENT PROTEIN KINASE AND DNA REPAIR IS DEPENDENT ON THE STRUCTURE OF DNA COFACTORS Derek S.
... in DNA repair and the DDR we sought to better understand the basic mechanism of DNA- PK activation The two primary components contributing to DNA- PK activation are protein/ protein and protein /DNA ... better understand how protein/ protein and protein /DNA interactions influence DNA- PK activation, we created and purified Ku80 C-terminal truncation mutants based on structural data and predicted ... Initiation of NHEJ requires the formation and activation of the DNA- dependent protein kinase (DNA- PK) Once activated, DNA- PK functions to regulate NHEJ pathway progression as well as initiates the DNA...
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báo cáo hóa học:" Sodium arsenite and hyperthermia modulate cisplatin-DNA damage responses and enhance platinum accumulation in murine metastatic ovarian cancer xenograft after hyperthermic intraperitoneal chemotherapy (HIPEC)" pptx
... the expression of DNA repair proteins and tumor platinum levels We show that NaAsO2 and hyperthermia either as single agents or in combination reverse key DNA repair protein responses to cisplatin ... than with hyperthermia treatment (CPA/43) Arsenic decreased to similar levels at 24 h Effect of cisplatin, arsenic and hyperthermia on DNA repair protein expression Cisplatin causes bulky DNA damage ... damage that is repaired mostly by the nucleotide excision repair system (NER) Cellular response to cisplatin- DNA damage involves the induction of DNA repair proteins to initiate DNA repair [10]...
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DNA REPAIR − ON THE PATHWAYS TO FIXING DNA DAMAGE AND ERRORS docx
... such as DNA replication, DNA repair and gene expression (reviewed in (Winkler and Luger, 2011)) Chromatin reconfiguration that occurs during embryonic DNA replication has a direct effect on reactivation ... located on the DNA and poised to carry both replication and repair functions, depending on the local environment and cellular requirements for normal functioning and survival The existence of proteins ... processing: modulation of the strand displacement activity of DNA polymerase delta by the concerted action of 20 DNA Repair − On the Pathways to Fixing DNA Damage and Errors replication protein A, proliferating...
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SINGLE MOLECULE STUDY ON DNA PROTEIN INTERACTION IN PROKARYOTES AND EUKARYOTES
... Interactions of Histone Proteins and DNA at Physiological Salt Concentrations Unlike the proteins which are negatively-charged, histone proteins have strong positive charges and thus it can bind to DNA ... stronger hysteresis (c-d) DNA- protein complexes respond to pH in 50 mM KCl and 100 nM protein concentrations Folding is much stronger at lower pH (6.5) and the DNA is hardly unfolded at this condition ... Histone chaperons are a group of proteins in eukaryotic cells to help chromatin assembly The binding mechanisms of histone chaperons to histone proteins and the functions of histone chaperones...
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Tài liệu Báo cáo khoa học: Effect of ionic strength and oxidation on the P-loop conformation of the protein tyrosine phosphatase-like phytase, PhyAsr docx
... Journal compilation ª 2008 FEBS 3785 Effect of ionic strength and oxidation on PhyAsr R J Gruninger et al Comparison of contacts to the P-loop in the unoxidized and oxidized conformations To accommodate ... based on the pKa values for InsP6 [30] The ionic strength P was calculated using the equation I = ½ ciZi2, where I is the ionic strength of the solution, and ci and Zi are the concentration and ... the general acid loop and active site arginine are sterically constrained; and (b) upon intramolecular bond formation Experimental procedures Purification and crystallization The S ruminantium...
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Tài liệu Báo cáo khoa học: DNA modification with cisplatin affects sequence-specific DNA binding of p53 and p73 proteins in a target site-dependent manner pptx
... in one strand and two AG steps in the other The mdm2 target, whose interaction with p53 was most strongly affected by DNA cis-platination, contains GG, GGG and AG motifs in one strand and GG and ... bifunctional cisplatin DNA adducts slow down or block DNA or RNA polymerization and can hamper the initiation of DNA transcription [49] Strong differential inhibition of marker gene expression was ... Journal compilation ª 2006 FEBS 4695 ˇ ´ H Pivonkova et al Cisplatin effects on p53 ⁄ p73 DNA recognition and p73d proteins to the DNA targets caused the formation of analogous retarded bands (denoted...
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Báo cáo khoa học: Plant DNA polymerase k, a DNA repair enzyme that functions in plant meristematic and meiotic tissues docx
... representation of a dRP lyase reaction A 21-mer double-strand oligonucleotide containing an abasic site at position 10 in one strand is treated with human AP endonuclease to release a dRP-containing ... proliferation Effects of DNA- damaging treatments on the levels of OsPol k expression In addition, we investigated the effects of DNA- damaging treatments, such as UV and methylmethanesulfonate (MMS), on ... not only in BER but also in DNA doublestrand break repair and meiosis, and Pol b and TdT may be evolved for differentiation and development of specific organs in vertebrates such as the nervous and...
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Báo cáo Y học: A novel DNA repair enzyme containing RNA recognition, G-patch and specific splicing factor 45-like motifs in the protozoan parasite Toxoplasma gondii potx
... exons, which was confirmed by the isolation and sequencing of the fulllength TgDRE cDNA The comparison of cDNA and genomic DNA nucleotide sequences allowed the determination of the exact position ... functions in the nuclear spliceosome and thus in RNA metabolism DISCUSSION In the present study, we report the isolation and expression of a cDNA encoding a novel DNA repair enzyme in T gondii ... DNA repair enzyme (DRT111) of Arabidopsis thaliana [9,10] Here, we report on the isolation of the gene locus, full-length cDNA and the functional analysis of the corresponding protein of T gondii...
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![Báo cáo khoa học: Recognition of DNA modified by trans-[PtCl2NH3(4hydroxymethylpyridine)] by tumor suppressor protein p53 and character of DNA adducts of this cytotoxic complex potx](https://media.store123doc.com/images/document/14/rc/cf/small_cfu1394954409.jpg)
Báo cáo khoa học: Recognition of DNA modified by trans-[PtCl2NH3(4hydroxymethylpyridine)] by tumor suppressor protein p53 and character of DNA adducts of this cytotoxic complex potx
... media, at low and high salt concentrations The effect on the melting temperature (tm) is dependent on the salt concentration At high salt concentration (0.05 m), modification of DNA by trans-PtHMP ... (2003) DNA binding by antitumor trans-[PtCl2(NH3)(thiazole)]: protein recognition and nucleotide excision repair of monofunctional adducts Biochemistry 42, 792–800 DNA recognition by p53 protein ... efficiency of the bifunctional mononuclear platinum(II) complex containing the leaving ligands in the trans configuration to inhibit binding of the p53 protein to its consensus DNA sequence Thus, in...
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Báo cáo khoa học: Association of RNA with the uracil-DNA-degrading factor has major conformational effects and is potentially involved in protein folding pot
... fraction contains RNA, and not DNA On native agarose gel, a smeared band was visualized in the nucleic acid-containing UDE fraction (Fig 1C) Test digestions of this UDE preparation by DNase as ... RNAUDE and UDE conformational states (Figs 3, 5, and S1) Under our experimental conditions, the distinct conformations RNA-dependent conformational states of UDE Fig Binding afnity of UDE for RNA and ... thermostatted at 25 C, with excitation and emission wavelengths of 295 and 340 nm, respectively, and excitation and emission slits set to and nm, respectively Protein (3 lm) in Tris buffer (the...
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Báo cáo khoa học: Fast set-up of doxycycline-inducible protein expression in human cell lines with a single plasmid based on Epstein– Barr virus replication and the simple tetracycline repressor ppt
... transcriptional activator (rtTA) [8] A third system [11] is based on concomitant expression of two fusion proteins, i.e rtTA2S-M2, which contains three tandem repeat VP16 minimal activation domains, and ... Plasmid constructs Fig Validation of pEBTet and pEBTetD vectors on the level of protein function (A) Regulation of expression ETTh, which resides in the plasma membrane Ergothioneine (ET) content ... Thus, relocation of TetR fusion proteins upon binding of inducer can be expected to cause secondary background differences Pronounced alteration of rtTA expression levels after addition of inducer...
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Báo cáo khoa học: Thermodynamic analysis of the unfolding and stability of the dimeric DNA-binding protein HU from the hyperthermophilic eubacterium Thermotoga maritima and its E34D mutant pdf
... various pH and protein concentrations) to the two-state model (Eqn 1) The Tm and DHU,m-values refer to the transition midpoint at the monomer concentration of 120 lM DGU,25 and DDGU,25 stand for ... aN, bN and aU, were adjustable According to Eqn (1) protein concentration will only affect the Tm values, whereas other parameters and thermodynamic functions of temperature are common and remain ... wild-type and the E34D mutant proteins were recorded at pH 4.00, 3.75 and 3.50 at monomer concentrations of 15, 30 and 60 lM (Fig 5B) It should be emphasized that the two latter concentrations were...
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Báo cáo khoa học: Helicobacter pylori single-stranded DNA binding protein – functional characterization and modulation of H. pylori DnaB helicase activity pptx
... replication, repair and recombination is single-stranded DNA binding protein (SSB) [16,17] The N-terminal domain of SSB is highly conserved and forms an oligonucleotide binding fold, and this region ... also responsible for oligomerization, typically homotetramerization in eubacteria The C-terminal region is less conserved and is responsible for protein protein interaction [18,19] The proteins ... phenylmethanesulfonyl fluoride and 10% glycerol For helicase and ATPase assays, HpSSB (Wt) and DC20SSB were dialysed against MonoQ and MonoS buffers and subjected to ion exchange chromatography using MonoQ and...
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Báo cáo khoa học: Structured DNA promotes phosphorylation of p53 by DNA-dependent protein kinase at serine 9 and threonine 18 doc
... structured single-stranded DNA (ssDNA) and hairpin DNA ends resembling replication and recombination intermediates [30,35] Preliminary studies indicated the phosphorylation of p53 by DNA- PK was dramatically ... of ssM13 and linearized double-stranded plasmid DNA (Fig 1) In the presence of ssDNA, substrate phosphorylation occurs in competition with DNA- PK autophosphorylation and autoinactivation [30] ... phosphorylation at Ser9 and Thr18 showed a strong preference for the colocalization of p53 and DNAPK on ssDNA This may explain why these sites have not been previously recognized as bona fide DNA- PK...
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Báo cáo hóa học: " Stretching and immobilization of DNA for studies of protein–DNA interactions at the single-molecule level" ppt
... combed DNA molecules The PADI (protein- assisted DNA immobilization) technique utilizes specific and non-specific interaction between DNA and DNA- binding proteins to stretch and immobilize DNA molecules ... immobilized DNA than on a coiled DNA, since along the former the protein follows a straight 1D path along the DNA Stretching DNA is also useful if one wishes to monitor its interactions with proteins ... of DNA attachment is demonstrated in Fig 16B and C At high -protein concentration, the DNA is firmly attached to the surface (Fig 16B), while lowering the protein concentration leads to fewer proteins...
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