chitinolytic enzymes from the moderately thermophilic bacterium ralstonia sp a 471 characterization and application
Báo cáo khoa học: Cloning, characterization and localization of a novel basic peroxidase gene from Catharanthus roseus potx
Ngày tải lên :
23/03/2014, 09:21
... AY032675 DQ650638 AY206412 AY206413 AF244923 NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA At5g40150 NA NA NA NA NA NA NA NA NA NA NA NA At5g05340 NA NA NA NA NA NA NA NA NA Unpublished Unpublished ... lanes 1, 3, and show the same using genomic DNA of C roseus Primer pairs were: #GSP-4 and #PFLF1 (lanes and 2); #GSP-2 and #GSP-4 (lanes and 4); #GSP-2 and #PFLR-1 (lanes and 6); and #PFLF-1 and ... (5¢-GGAATTCCCATG GCTTCCAAAAC) and PGFPR1 (5¢-GGACTAGTATG TAACTTATTAGCT-ACATAT) using Deep VentR DNA Polymerase (NEB) The amplified product contains NcoI and SpeI restriction enzyme cut sites, respectively...
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Báo cáo khoa học: Cloning and expression of a tomato cDNA encoding a methyl jasmonate cleaving esterase pdf
Ngày tải lên :
16/03/2014, 18:20
... cDNA for expression was cloned by another RT-PCR with primers fullMJEforMQ (GCATGCAGGGTGATAAAAA TCACTTTGTA) and fullMJErev (AAGGATCCATAA TATTTTTGCGAAATC), adding restriction sites for SphI and ... compounds and usually only JA content is measured If values of both JA and MeJA are published, the ratios depend on the plant species and the tissue analysed and vary from : (JA/MeJA) in Arabidopsis ... expression in spr-2 scions A strong candidate for a transmissible jasmonate signal is the JA-conjugate, MeJA The volatile ester can diffuse through membranes and can be found in the headspace above wounded...
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Báo cáo khoa học: cDNA cloning and characterization of a novel calmodulinlike protein from pearl oyster Pinctada fucata potx
Ngày tải lên :
16/03/2014, 23:20
... CaLP In the presence of calcium, oyster CaLP and CaM appeared as a single band with an apparent molecular weight of approximately 18 kDa and 14 kDa, respectively, whereas in the absence of calcium, ... 5¢-CTAATACGACTCACTATAGGGC AAGCAGTGGTAACAACGCAGAGT-3¢ and 5¢-CTAAT ACGACTCACTATAGGGC-3¢) and a reverse specific gene primer LSG1 In the second round of PCR reaction, the first round of PCR products then were used as the ... CaM and CaLP, while it reflected Ca2+-binding to the first and second EF-hand domains in the case of VanScyoc et al CaLP and CaM chromatography of extracts from oyster mantle and gills Fig Normalized...
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Báo cáo Y học: Cloning and expression of two novel aldo-keto reductases from Digitalis purpurea leaves potx
Ngày tải lên :
18/03/2014, 01:20
... Hordeum vulgare (P23901); Avena, Avena fatua (S61421); Sesbania, Sesbania rostrata (CAA11226); Oryza, Oryza sativa (AAK52545); Medicago, M sativa (X97606) AR proteins from mammals were used as the outgroup: ... and 1319 bp, designated DpAR1 and DpAR2, respectively The nucleotide sequences of DpAR1 and DpAR2 have been submitted to the EMBL database and are available under accession numbers AJ309822 and ... (this study); AAC23647, AAD32792, CAB88350 and AAC2346, Arabidopsis thaliana; Medicago, M sativa [16] The amino-acid residues identical to the DpAR1 sequence are indicated by dots Gaps are introduced...
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Báo cáo Y học: Identification and characterization of a novel activated RhoB binding protein containing a PDZ domain whose expression is specifically modulated in thyroid cells by cAMP pot
Ngày tải lên :
23/03/2014, 21:20
... display a cubical epithelial morphology correlated with a profound redistribution of both actin microfilaments and cytokeratin intermediate filaments, and with the appearance of a marked cytokeratin ... (1977) Analysis of singleand double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange Proc Natl Acad Sci USA 74, 4835–4838 Savonet, V., Maenhaut, C., ... stimulation, mRNA levels increased after 4–6 h and declined thereafter (Fig 5) To confirm that this increase of mRNA levels was secondary to the activation of the adenylyl cyclase/cAMP pathway, the...
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Cloning and characterization of a novel kelch like gene in zebrafish
Ngày tải lên :
03/10/2015, 20:57
... great potential to serve as a model for human disease that range from heart failure and vascular disease to fields as diverse as osteoporosis, renal failure, Parkinson’s disease, diabetes and cancer ... centrifugation at 12,000 g for 15 minutes at 4oC to separate the aqueous and organic phases 500 µl of aqueous phase was then transferred to a new tube and an equal amount of isopropanol was added The ... Especially to Ka-Leng, for always providing a listening ear, even when you’re miles away Special thanks also goes to Sandra, my first “Shifu” in the laboratory, for all the patience and guidance...
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Tài liệu Báo cáo khoa học: Identification, sequencing, and localization of a new carbonic anhydrase transcript from the hydrothermal vent tubeworm Riftia pachyptila docx
Ngày tải lên :
18/02/2014, 16:20
... 757–778 AAG AGC GCA ACT GAT AGT GCA TCC GCC ATC GAC GCA ATT AGC CTT GCT AGC AGT ACG AGG 613–630 822–839 706–723 466–483 AAG AGC AAA GAT GAT AGT GAT TAC GCC ATC CCA CAG ATT AGC ATC AAT AGC AGT CAG ... pseudoobscura, the mosquitoes A aegypti and A gambiae, the clam T gigas and larval sequences from the cnidarian F scutaria Finally, we chose an outgroup comprising a- CAs from a cyanobacteria (Nostoc sp. ) ... in the Archaea and Bacteria domains [11] Among the broad range of physiological processes in which they participate, CA can play a significant role in autotrophic organisms, serving as an inorganic...
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Báo cáo khoa học: Detection and characterization of a novel extracellular fungal enzyme that catalyzes the specific and hydrolytic cleavage of lignin guaiacylglycerol b-aryl ether linkages pdf
Ngày tải lên :
17/03/2014, 03:20
... indicated that the b-aryl ether cleavage enzyme accumulated and was stable in the extracellular fraction The extracellular fraction of 2BW-1 generated abundant GG and 4MU from GOU by cleaving the ... further experiments Purification and characterization of the b-aryl ether cleavage enzyme Reaction mechanism and substrate specificity To characterize in further detail the b-aryl ether cleavage ... a new enzyme catalyzing the beta-aryl ether cleavage in the soil bacterium (Pseudomonas paucimobilis SYK-6) FEBS Lett 249, 348–352 13 Masai, E., Katayama, Y., Kawai, S., Nishikawa, S., Yamasaki,...
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Báo cáo khoa học: Proteome analysis of a rat liver nuclear insoluble protein fraction and localization of a novel protein, ISP36, to compartments in the interchromatin space pptx
Ngày tải lên :
30/03/2014, 20:20
... and characterization of a novel zinc finger protein that associates with nuclear matrix DNA Cell Biol 17, 849–858 Lee JY, Nakane Y, Koshikawa N, Nakayama K, Hayashi M & Takenaga K (2000) Characterization ... after ISP36 induction, respectively A3 contains lg of His6-ISP36 purified by Ni-agarose beads A1 A3 are stained with Coomassie Brilliant Blue A4 A6 are the same as A1 A3 except that the samples ... prophase, ISP36 staining was partly lost in the interchromatin regions but accumulated in a limited area (Fig 9, panel 5) In prometaphase and metaphase, ISP36 staining was excluded from the interchromatin...
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báo cáo hóa học: " The design and testing of a novel mechanomyogram-driven switch controlled by small eyebrow movements" docx
Ngày tải lên :
19/06/2014, 08:20
... Scholarship, Natural Sciences and Engineering Research Council of Canada and the Canada Research Chairs program The authors acknowledge Mr Ka Lun Tam for his implementing the hardware interfaces, and Mr ... as demonstrated by qualitative participant feedback and the trends in response time Further, as a control site, the frontalis muscle is broad and has a large surface area on the forehead, thus ... factor was selectable in the 1.2-2.5 range, and was adjusted for each participant such that false activations due to blinks and movement were avoided and participants were able to activate the switch...
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báo cáo hóa học: "Initial development and testing of a novel foam-based pressure sensor for wearable sensing" doc
Ngày tải lên :
19/06/2014, 10:20
... evaluations, and drafted the manuscript SB created the foam sensors, participated in the prototype pilot evaluations, and drafted the manuscript BS participated in the project organization and ... were attached at the outer edge of the garment at the apex of each shoulder (above the subject's axilla) The sensors measured 1.5 × 2.0 × 0.5 cm Data was gathered with the subject seated, and the ... needs and wants of the user A subtle, comfortable sensor that demands no attention or adaptation from the user can allow the application to function invisibly, reducing the cognitive load on the...
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Báo cáo y học: "The identification and characterization of a novel protein, c19orf10, in the synovium" docx
Ngày tải lên :
09/08/2014, 10:20
... to the maintenance of synovial inflammation, aggravating the destruction of cartilage and bone and stimulating the development of the pannus Because FLSs can exhibit significant phenotypic changes ... containing the c19orf10 c19orf10 gene is indicated on the ideogram The area containing the c19orf10 gene is expanded and the base-pair positions are indicated The location of a microsatellite marker ... similar patterns of reactivity in a comparative staining analysis These antibodies were all of the IgM class and this may be attributable to the fact that there is 91% identity between the predicted...
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báo cáo khoa học: " The isolation and mapping of a novel hydroxycinnamoyltransferase in the globe artichoke chlorogenic acid pathway" pptx
Ngày tải lên :
12/08/2014, 03:20
... GGGTTTCATATGACTATCGGAGCTCGTGAT CGGGATCCCTAGAAGTCATACAAGCATTT TTTTTAAGCTAACACGAGAC TCTCATAGGAGCTGTAATTG TAAAATGGACGATCAGTATC TTATGTTCAGATTTGGACTC TACTTTCTACAACGAGCTTC ACATGATTTGAGTCATCTTC GGGTTTCATATGAAGATCGAGGTGAGAGAA ... CGGGATCCTTAGATATCATATAGGAACTTGC ATATTCACGACGACTCCGATAGCGGTATCG CACGTCGGCTTCGACTGTAGGTCGACT CACGAGACCAAGTCAATGCACTCAAAGGA GATTCGGGCACTTAAACGTATGAGCCCC CGTGGACTATCAGACGATCAACCATCC TCGTCCGTCAGTAGCCACGTACAGTATC ... between the parents, allowing the alignment of their LG5 The HQT locus maps close to AFLP markers e38/m47-01 and e47/m49-06 in the female map, and to the M-AFLP marker polyGA/e33-02 and the microsatellite...
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Báo cáo khoa học: "Identification and characterisation of a novel anti-viral peptide against avian influenza virus H9N" pps
Ngày tải lên :
12/08/2014, 04:21
... GAGTCACTTTAAAATTTGTATACAC 3' 5' GATGTTAACGATACCAGCC 3' 5' GCGTGAATGTAAGCGTGAC 3' 5'ATTTAAGGATCCGAGAGCCATGGA 3' 5'ATGCTGCTCGAGTTATATACAAATGTTGC 3' 5'CATAGAATTCGCAAAAGCAGGAGT 3' 5'TATCGCTCGAGAGTAGAAACAAGGAG ... TATCGCTCGAGAGTAGAAACAAGGAG 3' 5' ATTTAAGGTACCGACAGCCATGGA 3' 5' ATGCTGCTCGAGTATACAAATGTTGC 3' 5' AGCCTGGAATTCATGAAAAAATTA 3' 5' ATCGAACTCGAGATTTTCAGGGAT 3' 5' AGGGCTGGCGGTTGGGGGTTATTCGC 3' 5' GAGTCACTTTAAAATTTGTATACAC ... that H9N2 prevalence in poultry pose a significant threat to humans [5-8] Adamantane derivatives (amantadine and rimantadine) and neuraminidase inhibitors (NAIs; zanamivir and oseltamivir) are...
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Báo cáo y học: "Quantifying the major mechanisms of recent gene duplications in the human and mouse genomes: a novel strategy to estimate gene duplication rates" docx
Ngày tải lên :
14/08/2014, 08:20
... Additional data files The following additional data are available with the online version of this paper Additional data file provides the human ALL gene set Additional data file provides the mouse ALL ... Rogers J, Abril JF, Agarwal P, Agarwala R, Ainscough R, Alexandersson M, An P, et al.: Initial sequencing and comparative analysis of the mouse genome Nature 2002, 420:520-562 Thomas JW, Touchman JW, ... denoted as the FAM2 gene set There are 1,364 and 1,323 gene pairs in human and mouse, respectively, in the FAM2 gene set deposited research TAGs are tandemly arrayed genes that belong to the same...
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TARGETING ACUTE PHOSPHATASE PTEN INHIBITION AND INVESTIGATION OF A NOVEL COMBINATION TREATMENT WITH SCHWANN CELL TRANSPLANTATION TO PROMOTE SPINAL CORD INJURY REPAIR IN RATS
Ngày tải lên :
24/08/2014, 09:14
... towards the more important goals in graduate research training and for all the advice and suggestions to attain them This guidance will always be remembered and appreciated iii ABSTRACT Chandler ... vascular quantification using a general rat endothelial cell marker, and calculation of the vessel-positive area was performed in the ipsilateral and contralateral gray matter in vehicle- and bpV-treated ... improving SC transplantation for SCI In spite of the many advantages of SC transplantation, there are certain limitations that must be overcome to further the potential for SCs as a viable therapeutic...
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Development and evaluation of a novel nanoparticulate delivery system of arsenic sulfides
Ngày tải lên :
11/09/2015, 16:03
... physician and founder of chemotherapy, developed an organic arsenical, Salvarsan (Arsphenamine), which was effective in treating tuberculosis and syphilis Arsphenamine was the standard therapy for ... and larger mammals and even transgenic animals, all models have failed to elucidate satisfactorily the actual mechanisms of arsenic carcinogenicity Despite the hazards, the potential for adverse ... in the early treatment phase Mean age at diagnosis is about 40 years The male to female ratio is balanced [Groopman J and Ellman L, 1979] APL is characterized by rapid accumulation of immature...
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Identifcation and stablization of a novel 3d hepatocyte monolayer for hepatocyte based applications
Ngày tải lên :
12/09/2015, 08:19
... are separated from plasma by the capillary membranes Before entering the bioreactor, the plasma passes a charcoal absorber for detoxification and a membrane oxygenator for oxygen enrichment The ... 3-MC 3-methylcholanthrene AHG 1-O-(6’-aminohexyl)-D-galactopyranoside AMC Academic Medical Center ALF Acute liver failure ALT Alanine Transaminase APAP Acetaminophen ASGPR Asialoglycoprotein Receptor ... loss is also a critical issue in forming and maintaining these spheroids in applications since the spheroids detach easily from the substratum[113, 114] 1.3 Natural and synthetic biomaterials for...
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Identification and characterization of a novel heart reactive autoantibody in systemic lupus erythematosus possible serological marker for early myocardial dysfunction
Ngày tải lên :
14/09/2015, 12:42
... incidence was found in Iceland and Japan and highest in USA and France The overall prevalence was the lowest in Northern Ireland, UK and Finland, and the highest in Italy, Spain and Martinique The burden ... aboriginal populations in Australia and New Zealand report higher prevalence and incidence in this population than in Australian Caucasians Both racial and genetic differences, and more complex social ... anticholesterol antibody aCL anticardiolipin antibodies ANA antinuclear autoantibody ANP atrial natriuretic peptide APS anti-phospholipid syndrome aPL anti-phospholipids antibodies ARA American Rheumatology...
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