chemical modification of small interfering rna
Báo cáo khoa học: Gene expression silencing with ‘specific’ small interfering RNA goes beyond specificity – a study of key parameters to take into account in the onset of small interfering RNA off-target effects potx
... SREBF1 SREBF1 NT siRNA SREBF1 SREBF1 NT siRNA SREBF1 SREBF1 NT siRNA siRNA1 siRNA2 (nM) siRNA1 siRNA2 (nM) siRNA1 siRNA2 (nM) (nM) (nM) (nM) (nM) (nM) (nM) B Relative SREBF1 mRNA abundance S Vankoningsloo ... 100 20 100 20 LAMP2 siRNA1 (nM) LAMP2 NT siRNA siRNA2 (nM) (nM) LAMP2 siRNA1 (nM) LAMP2 NT siRNA siRNA2 (nM) (nM) LAMP2 siRNA1 (nM) LAMP2 NT siRNA siRNA2 (nM) (nM) Fig Effect of the SREBF1-targeting ... Journal 275 (2008) 2738–2753 ª 2008 The Authors Journal compilation ª 2008 FEBS 2743 S Vankoningsloo et al DF LAMP2-siRNA1 LAMP2-siRNA2 NT_siRNA DF SREBF1-siRNA1 SREBF1-siRNA2 NT_siRNA siRNA off-target...
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Báo cáo y học: " Efficient delivery of small interfering RNA for inhibition of IL-12p40 expression in viv" pdf
... mammalian cells by the presence of dsRNA or in the presence of short 19–22nt dsRNA fragments termed small interfering RNA molecules (siRNA) siRNA molecules activate an RNA- induced silencing complex ... Journal of Inflammation 2004, 1:4 on the ability of double stranded RNA (dsRNA) to specifically degrade homologous messenger RNA (mRNA) The RNAi pathway is triggered in ... Journal of Inflammation 2004, 1:4 http://www.journal-inflammation.com/content/1/1/4 Silencing 3of IL-12 by siRNA interferes with the inflammatory response in vivo Figure Silencing of IL-12 by siRNA...
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Báo cáo y học: " Inhibitory effect of small interfering RNA on dengue virus replication in mosquito cells" ppsx
... [22-24] The mixtures of DEN specific small interfering RNAs, the hallmark of RNAi, were detected in all aforementioned studies But little was known about the role of single siRNA with particular ... prevention and treatment of DEN infection The stability and inhibitory efficiency of siRNA need further improvement in the future Page of siRNA design and synthesis Four pairs of siRNAs against different ... T: RNA interference is mediated by 21and 22-nucleotide RNAs Genes Dev 2001, 15:188-200 Chiu YL, Rana TM: RNAi in human cells: basic structural and functional features of small interfering RNA...
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Báo cáo y học: "Allele dependent silencing of COL1A2 using small interfering RNAs"
... of cDNA samples derived from RNA isolated 72h post-transfection with: (A) siRNA3 (B) Negative Control siRNA FIGURE Allele ratios of the two COL1A2 mRNA alleles (normalized cDNA peak heights of ... 82% (Figure 5B), of which 75%, 75% and 73% could be attributed to silencing of the targeted T-allele, respectively Assessment of relative allele abundance of COL1A2 mRNA The software PeakPicker ... sites in mRNA as well as on the siRNA sequences themselves It will be necessary to analyze allele specificity and off-target effects of a large siRNA subset which target the full array of COL1A1...
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Báo cáo hóa học: " Inhibition of foot-and-mouth disease virus replication in vitro and in vivo by small interfering RNA" pot
... Inhibition of foot-and-mouth disease virus replication by small interfering RNA Journal of General Virology 2004, 85:3213-3217 Publish with Bio Med Central and every scientist can read your work free of ... maintain the siRNA target sequences without alterations, ensuring the effective activity of the siRNAs described in the present study This work offers an insight into the use of RNAi in animal ... Only of 9–10 mice pretreated with pSi-FMD2 and of 10 mice pretreated with pSiFMD3, survived a viral challenge of LD50 for days of observation Further, only of mice pretreated with pSiFMD2 and of...
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báo cáo hóa học:" In vitro suppression of the MMP-3 gene in normal and cytokine-treated human chondrosarcoma using small interfering RNA" doc
... gene therapy offers novel approaches to the medical management of OA [3,4] One of the latest techniques is RNA interference (RNAi), which is widely used to downregulate the mRNA level of a particular ... [5,6] The mediators of sequence-specific messenger RNA degradation are 21- and 22-nucleotide small interfering RNAs (siRNAs) generated by ribonuclease III cleavage from longer dsRNAs [7-9] In this ... level of HA also increased 400% and 600% in 48 h and 72 h cultures, respectively The effect of siRNA on target mRNA expression The specificity of MMP-3 siRNA was determined by transfection of non-targeted...
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báo cáo khoa học: "Adenovirus-mediated delivery of bFGF small interfering RNA reduces STAT3 phosphorylation and induces the depolarization of mitochondria and apoptosis in glioma cells U251" pdf
... Adenovirus-mediated delivery of bFGF small interfering RNA reduces STAT3 phosphorylation and induces the depolarization of mitochondria and apoptosis in glioma cells U251 Journal of Experimental & Clinical ... Journal of Experimental & Clinical Cancer Research 2011, 30:80 http://www.jeccr.com/content/30/1/80 Page of Figure Ad-bFGF-siRNA reduces the activation of upstream molecules and the expression of ... accumulation of basic fibroblast growth factor in human astrocytic tumors Cancer 2003, 97:3061-3067 Zhang B, Feng X, Wang J: Adenovirus-mediated delivery of bFGF small interfering RNA increases levels of...
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Báo cáo y học: " Inhibition of HIV-1 replication by small interfering RNAs directed against Glioma Pathogenesis Related Protein (GliPR) expression" potx
... HIV-1 infection was performed 24 h post siRNA transfection with a MOI of Figure Efficacy of siRNA-mediated suppression of GliPR (A) Quantitative PCR analysis of GliPR expression in P4-CCR5 cells which ... experiment, approximately 70% of Jurkat cells were transfected, while 90% of P4-CCR5 HeLa cells were transfected Page of 10 In general, GliPR-directed siRNAs reduced the expression of GliPR effectively ... in this early phase Suppression of GliPR mediated by short interfering RNA P4-CCR5 cells were transfected with siRNAs specific for GliPR or a non-silencing siRNA, which was 5-prime labeled with...
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Báo cáo sinh học: "Prevention of airway inflammation with topical cream containing imiquimod and small interfering RNA for natriuretic peptide receptor" doc
... chitosan delivery of vector-driven small interfering RNA (siRNA) intranasally to protect mice from respiratory syncytial virus infection [3] While oral and intranasal routes of drug delivery are ... openings of the skin are 30 to 100 μm, it is reasonable to expect that nanocomplexes would facilitate the penetration through the skin of DNA or small oligonucleotides such as siRNAs [12] siRNAs ... SiRNA: siGLO and siNPRAs As siRNA marker, siGLO green indicator was purchased from Dharmacon Research Inc For siRNA against NPRA, several targeting sequences were located using siRNA finder software...
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Báo cáo khoa học: Treatment with small interfering RNA affects the microRNA pathway and causes unspecific defects in zebrafish embryos doc
... pg pg pg of of of of of of of of of of miR-430b miR-206 siEri1 + 250 pg of miR-430b siEri1 + 250 of pg miR-430b-mis siEri1 + 250 pg of miR-206 siGFP + 250 pg of miR-430b siGFP + 450 pg of miR-430b ... facilitates generation of siRNAs and mature miRNAs from dsRNAs and pre-miRNAs, respectively [13,14] Accordingly, the injection of large amounts of siRNAs would affect the availability of these factors ... regulatory function of miR-430 revealed that these miRNAs accelerate deadenylation and degradation of several hundred different types of maternal mRNAs, leading to a sharpening of the maternal-to-zygotic...
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Báo cáo y học: "Effect of small interference RNA (siRNA) for ADAMTS5 on intervertebral disc degeneration in the rabbit anular needle-puncture model" pptx
... model RNA (siRNA) or ADAMTS5 siRNA injection of control puncture reflecting typical histologic Safranin-O-stained small interfering of disc degenerationchanges after injection of control small interfering ... disc degeneration interfering RNA (siRNA) injection in assessment weeks after small Magnetic resonance imaging (MRI) the rabbit anular puncture model of interfering RNA (siRNA) injection in the ... cells that received the ADAMTS5 siRNA oligonucleotide showed approximately a Page of 10 (page number not for citation purposes) ADAMTS5 in of small interfering RNA (siRNA) oligonucleotide for Establishmentrabbit...
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Báo cáo y học: "Small interfering RNA mediated Poly (ADP-ribose) Polymerase-1 inhibition upregulates the heat shock response in a murine fibroblast cell line" pptx
... on line software (Block-iT™RNAi Express) to target PARP1 mRNA (accession number NM007415) Small interfering RNA (siRNA)-mediated silencing of the PARP-1 gene was performed using 21-bp siRNA duplexes ... calculate mRNA levels for HSP-70 in the samples Results were normalized relative to 18 S rRNA expression SiRNA-mediated inhibition of PARP expression Stealth small interference RNA (siRNA) sequences ... University of Pittsburgh School of Medicine and Children’s Hospital of Pittsburgh, Pittsburgh, PA 15213, USA 2Department of Critical Care Medicine, University of Pittsburgh School of Medicine...
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Báo cáo y học: " Small interfering RNA mediated Poly (ADP-ribose) Polymerase-1 inhibition upregulates the heat shock response in a murine fibroblast cell line." pptx
... on line software (Block-iT™RNAi Express) to target PARP1 mRNA (accession number NM007415) Small interfering RNA (siRNA)-mediated silencing of the PARP-1 gene was performed using 21-bp siRNA duplexes ... calculate mRNA levels for HSP-70 in the samples Results were normalized relative to 18 S rRNA expression SiRNA-mediated inhibition of PARP expression Stealth small interference RNA (siRNA) sequences ... University of Pittsburgh School of Medicine and Children’s Hospital of Pittsburgh, Pittsburgh, PA 15213, USA 2Department of Critical Care Medicine, University of Pittsburgh School of Medicine...
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Báo cáo khoa học: Biochemical characterization of a U6 small nuclear RNA-specific terminal uridylyltransferase potx
... incorporation of GMP residues into the opposite 3¢-end of the mutant RNA A second mutation of the U6 substrate RNA consisted of the introduction of two additional AMP nucleotides in front of the oligo(A) ... mutants of the U6 snRNA (A) Proposed secondary structure of U6 snRNA [25] Mutant RNAs were cloned by insertion of CMP or AMP nucleotides into the internal oligo(A) stretch (+27/+30) of the wild-type ... in the presence of lg total cellular RNA as substrate Taking into account the low concentration of U6 snRNA in total cellular RNA, even the smallest amounts of oligo(A)/U6-3 RNA coeluted from...
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In vitro and in vivo targeted delivery of IL-10 interfering RNA by JC virus-like particles pptx
... for 48 h by IL-10 shRNA Thus, we found suppression of IL-10 by VLPs-IL-10 shRNA not only at mRNA levels but also at protein level Effects of IL-10 shRNA on cytokines production of IL-10 and TNF-α ... Represented are the mRNA levels of target gene relative to those of 18 s rRNA (A) Suppression of IL-10 expression was analyzed by real-time PCR (B) IL-10 shRNA did not cross-react with TNF-α mRNA and influence ... since RNAi is mainly functioning on mRNA level of target gene and real-time PCR is a sensitive method to quantitate the level of mRNA, we are confident that IL-10 shRNA did not react with TNF-α mRNA...
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báo cáo khoa học: " Effects of metastasis-associated in colon cancer 1 inhibition by small hairpin RNA on ovarian carcinoma OVCAR-3 cells" pot
... phosphoinositide 3-kinase; RNAi: RNA interference; shRNA: small hairpin RNA Acknowledgements We thank Qinxian Zhang who was from Department of Organization and Embryology of Basic Medical College of Zhengzhou ... Turner DL: RNA interference by expression of short interfering RNAs and hairpin RNAs in mammalian cells Proc Natl Acad Sci USA 2002, 99:6047-6052 10 Osborne CK, Hobbs K, Clark GM: Effect of estrogens ... control cells, levels of MACC1 mRNA and protein were significantly Suppression of migration by MACC1 RNAi Compared with control groups, OVCAR-3-s3 cells showed suppressed capacity of impaired migration...
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báo cáo khoa học: "In Medicago truncatula, water deficit modulates the transcript accumulation of components of small RNA pathways" pptx
... The mRNA levels of MtAGO11 (a protein similar to AtAGO6 - Figure 1, A) could not be quantified AtAGO7 is involved in the biogenesis of trans-acting small RNAs (ta-siRNAs) derived from TAS3 RNA ... enzymes, RNA- dependent RNA polymerase (RDR6), Suppressor of gene silencing (SGS3) and Silencing Defective (SDE5) are involved in double strand RNA (dsRNA) production from the cleaved mRNA of AGO1 ... The processing and activation of miRNAs are up regulated as well as the sRNAs mediated DNA methylation mechanisms and the production of transacting small interfering RNAs Our observations opened...
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Báo cáo khoa học: "Small interference RNA profiling reveals the essential role of human membrane trafficking genes in mediating the infectious entry of dengue virus" pdf
... 50 μM of siRNA with scrambled sequence of PTB A Z’ factor of 0.70 was consistently observed, demonstrating the reliability and robustness of this assay With the optimization of this siRNA screening ... due to off-target gene effects of the siRNA primary screen 30 nM of each Ang et al Virology Journal 2010, 7:24 http://www.virologyj.com/content/7/1/24 Page of 17 Figure Identification of human ... discovery of cellular factors that is essential for mediating the infectious entry process of DENV into cells siRNA profiling of human membrane trafficking genes required for DENV infection An array of...
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Báo cáo y học: " Dynamic expression of small non-coding RNAs, including novel microRNAs and piRNAs/21U-RNAs, during " pps
... reads miRNA 21U -RNA Figure of miRNA and 21U -RNA reads at each developmental stage of hermaphrodites and in males Proportions Proportions of miRNA and 21U -RNA reads at each developmental stage of hermaphrodites ... U U U U mir-2217 Figure Validation of the expression of novel miRNAs Validation of the expression of novel miRNAs (a) Validation of the expression of novel miRNAs by RT-PCR Error bars represent ... Identification of larger reads corresponding to piRNAs/ 21U-RNAs Of the 562 novel piRNAs/21U-RNAs we identified, 438 partially overlap other 21U-RNAs; either of their termini is located within 10 bp of another...
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Fabrication of nanopatterns via surface chemical modification and reactive reversal nanoimprint lithography
... concentration of functional monomers to be evenly distributed over the imprinted array This method of fabricating polymeric support patterns offers a high degree of freedom in terms of the choice of chemical ... limit of ~10 nm.53 The use of computers facilitates the automation of certain operations, both for the administration of SEM parameters and focus or for the interface of design and the control of ... soft-lithography) prove to be a useful alternative for the fabrication of chemical and topological structures.46-50 1.1.2 Electron beam lithography EBL makes use of an electron beam instead of...
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