characterization of the b cell clone

Báo cáo y học: " Ageing, autoimmunity and arthritis: Senescence of the B cell compartment — implications for humoral immunity" pptx

Ngày tải lên : 09/08/2014, 01:23

... marks the transition to the immature B cell stage Immature B cells are the earliest cells in the lineage that express a bona fide antigen specific B cell receptor (BCR), and therefore they are the ... gradual loss of naïve follicular B cells, but not of MZ or B1 B cells Recent studies conducted in our laboratory also suggest that reduced influx of B cells from the BM drives the selection of antigen-experienced ... CD5+ B cell expansions found in the periphery of aged animals are not found among B cell precursors in the BM [37] Thus, it has been hypothesized that these cells develop in the periphery, probably...

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Báo cáo y học: " Successful long-term monotherapy with rituximab in a patient with chronic lymphocytic leukemia of the B-cell-lineage: a case report" pdf

Ngày tải lên : 11/08/2014, 21:22

... pancytopenia Therapy with alemtuzumab was considered, but preference was given to rituximab because of the less severe compromise of the cellular immune system by rituximab, and because of the possibility ... completely Over the entire term of maintenance therapy, treatment of pulmonary infections by oral antibiotics became necessary three times but could be performed at the outpatient level Due to the good ... rituximab 375 and leucocyte Course of Course of hemoglobin, leucocyte and thrombocyte numbers under therapy with chlorambucil, fludarabine, cyclophosphamide and rituximab 375 and 600 mg/m2 Page of...

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Tài liệu Báo cáo khoa học: Identiﬁcation and characterization of the transcription factors involved in T-cell development, t-bet, stat6 and foxp3, within the zebraﬁsh, Danio rerio docx

Ngày tải lên : 16/02/2014, 09:20

... TBX2 ZEBRAFISH TBX2 XENOPUSTR TBX2 MOUSE TBX3 HUMANTBX3 CHICKEN TBX3 HUMAN TBX6 MOUSE TBX6 XENOPUSTR TBX6 ZEBRAFISH TBX6 HUMAN T -BET MOUSE T -BET ZEBRAFISH T -BET GINBUNACARP T -BET MOUSE TBX20 ... HUMAN TBX20 CHICKEN TBX20 XENOPUSTR TBX20 ZEBRAFISH TBX20 MOUSE TBX15 HUMAN TBX15 MOUSE TBX18 HUMAN TBX18 MOUSE TBX1 HUMAN TBX1 XENOPUSTR TBX1 MOUSE TBX10 HUMAN TBX10 MOUSE TBX5 RAT TBX5 HUMAN TBX5 ... CHICKEN TBX5 XENOPUSLA TBX5 ZEBRAFISH TBX5 DOG TBX4 HUMAN TBX4 TBOX -2/-3 2/- TBOX -6/-21 TBOX-20 TBOXTBOX -15/-18 TBOX-1/-10 TBOX- 1/- TBOX -4/-5 Fig Unrooted phylogenetic tree showing the relationship...

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Báo cáo Y học: Baboon cytochrome P450 17a-hydroxylase/17,20-lyase (CYP17) Characterization of the adrenal microsomal and cloned enzymes docx

Ngày tải lên : 08/03/2014, 09:20

... the metabolism of PREG by the two enzyme preparations Despite the large degree of homology between the human and baboon CYP17 the expressed baboon CYP17 was unable to catalyse the formation of ... conversion of PREG by baboon CYP17 A comparison of the ratios of DHEA:17-OHPREG formation during PREG metabolism indicated that the biosynthesis of DHEA was initially slower for baboon CYP17 than for the ... ampliﬁcation of baboon mRNA, using baboon speciﬁc primers complementary to the nucleotide sequence encoding the amino and carboxy terminal of baboon 5612 A C Swart et al (Eur J Biochem 269) Ó FEBS 2002...

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Báo cáo khoa học: Characterization of the bga1-encoded glycoside hydrolase family 35 b-galactosidase of Hypocrea jecorina with galacto-b-D-galactanase activity pdf

Ngày tải lên : 16/03/2014, 11:20

... in Bga1 All these positions are located at the surface of the jelly roll domain of Bga1 and thus most probably are glycosylated in vivo To further substantiate these findings, in gel digestion of ... that of d-galactose The degree of transglycosylation was dependent on the concentration of the substrate, as also found in other studies [12,13] Activity of Bga1 on polymeric substrates The ability ... preference: based on the ratio of kcat ⁄ Km, the aromatic artificial galactoside oNPG was the most preferred substrate, which may indicate a beneficial role of hydrophobicity in one of the steps of the...

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Báo cáo khoa học: Spectroscopic characterization of the isolated heme-bound PAS-B domain of neuronal PAS domain protein 2 associated with circadian rhythms doc

Ngày tải lên : 16/03/2014, 22:20

... between amino acids 347 and 354 of mouse NPAS2, which were absent in the studies by Dioum et al make up part of the core of the PAS -B domain [27,28] Therefore, the lack of these residues in the ... heme-binding PAS proteins If the PAS -B domain functions only in the heme binding, then it can be speculated that the regulation of transcription by NPAS2 may be modulated not only by CO binding but ... determined the dissociation rate of heme from Fe(III) PAS -B by adding an excess of the His64Tyr ⁄ Val68Phe apomyoglobin mutant [18] The formation of Fe(III)-bound myoglobin was monitored by following the...

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Báo cáo khoa học: Quenched hydrogen ⁄deuterium exchange NMR characterization of amyloid-b peptide aggregates formed in the presence of Cu2+ or Zn2+ ppt

Ngày tải lên : 30/03/2014, 01:20

... relevant, but the most abundant are Ab(1–40) and Ab(1–42) [2] The fold and mode of assembly of Abs are determined by their primary sequences in combination with the chemical properties of the solvent ... they are deeply buried in the core of the fibril [12,13] Samples verified by AFM and ThT analysis to contain the fibrillar forms of Ab(1–40) and Ab(1–42) (fibrils formed in the presence of EDTA) displayed ... [19,28] During the time of the analysis, no detectable turbidity was observed for either Ab(1–40) or Ab(1–42) in the absence of divalent metals, i.e when EDTA was included in the reaction mixture...

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Báo cáo khoa học: Characterization of the lipopolysaccharide and b-glucan of the ﬁsh pathogen Francisella victoria ppt

Ngày tải lên : 30/03/2014, 10:20

... understanding of the molecular basis of their biological properties, the host speciﬁcity and diversity of members of this important bacterial genus, as well as the nature of its pathogenicity The results ... of NOE and HMBC correlations (Table 1) A very large number of the NOE correlations from the H-6 of the 6-deoxysugars was observed, and most of them could be rationalized within the proposed structures ... to the observation of a signal at m ⁄ z 199.2, corresponding to a cyclic AA component The cyclic structure of the AA was conﬁrmed by the observation of the weak C-5: H-2 HMBC correlation There...

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Báo cáo khoa học: Expression and characterization of soluble forms of the extracellular domains of the b, c and e subunits of the human muscle acetylcholine receptor pot

Ngày tải lên : 30/03/2014, 10:20

... unavailability of a conformation-dependent e subunit mAb, only binding of antiFLAG mAb was tested CD spectra Binding of the rabbit anti -b serum to recombinant b1 –221 and human AChR When the b subunit ... using the conformationdependent mAbs 73 (binds to an epitope on the extracellular side of the b subunit) [22] and 67 (binds to an epitope on the extracellular side of the c subunit) [22] and the ... conﬁrming the FPLC data, considering that the ‘height’ of the ECD of the AChR is nm [3] This difference in solubility between the c–e and the b ECDs might be attributed to the primary structure of the...

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Báo cáo khoa học: Characterization of the 5¢ untranslated region of a and b isoforms of the human thromboxane A2 receptor (TP) Differential promoter utilization by the TP isoforms doc

Ngày tải lên : 31/03/2014, 09:20

... to the site of TI of the TPb mRNA Thereafter, through a number of independent approaches employing 5¢ RACE, it was established that the major TI of the TPb transcripts in both HEL and TM-1 cells ... Kinsella, B. T (2000) Investigation of the role of the carboxyl terminal tails of the a and b isoforms of the human thromboxane A2 receptor (TP) in mediating receptor: eﬀector coupling Biochem Biophys ... number of the diverse superfamily of GPCRs, not least among the prostanoid receptor subfamily of GPCRs [53] The EP3 subtype of the PGE2 receptors is one of the most heavily spliced among all of...

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Báo cáo hóa học: " Characterization of the IFN-γ T-cell responses to immediate early antigens in humans with genital herpes" doc

Ngày tải lên : 20/06/2014, 01:20

... the bead-bound CD8 cells and 1717 for free CD8 cells indicating that the bead bound CD8 cells and the free CD8 cells generate the same response under these assay conditions The ability of CD8 cells ... depletion of CD8 cells byin PBMC samples Flow cytometry of CD8 T -cell populations in PBMC samples with and without depletion of CD8 cells by magnetic beads PBMC samples were depleted of CD8 cells ... media The cells were suspended at a concentration corresponding to million PBMCs/ml based on the original input of PBMCs The magnetic bead bound PBMC fraction was washed twice in 2%FBS/PBS and then...

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báo cáo hóa học:" Characterization of the tumor marker muc16 (ca125) expressed by murine ovarian tumor cell lines and identification of a panel of cross-reactive monoclonal antibodies" docx

Ngày tải lên : 20/06/2014, 07:20

... influence on the cytolytic function of natural killer cells, the cell surface bound MUC16 is important for binding of the ovarian tumor cells to the mesothelial cells that line the peritoneal ... intracellular Muc16 in the four MOVCAR cell lines was determined by flow cytometry using the 618 F antibody The mean fluorescence intensity for the binding of 618F to the cell surface and intracellular ... distinctions between the human and murine forms of the mucin MUC16 is both expressed on the cell surface and shed from the cell in soluble forms Muc16, on the other hand, is detected primarily in the...

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Molecular characterization of the PhoPQ-PmrDPmrAB mediated pathway regulating polymyxin B resistance in Klebsiella pneumoniae CG43 ppt

Ngày tải lên : 10/08/2014, 05:21

... amount of CPS was protective for the bacterial resistance to polymyxin B On the other hand, the deletion of ugd resulted in the loss of resistance to polymyxin B Sequence analysis of the available ... to determine the number of viable bacteria The survival rates were expressed as colony counts divided by the number of the same culture treated with PBS and multiplied by 100 The assays were performed ... effect of pmrF in Page of 16 polymyxin B resistance was observed, and the survival rate could be restored by the introduction of pRK415PmrF The results indicated a role of PmrF in the polymyxin B...

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Báo cáo y học: "Genetic characterization of the cell-adapted PanAsia strain of foot-and-mouth disease virus O/Fujian/CHA/5/99 isolated from swine" doc

Ngày tải lên : 12/08/2014, 01:21

... (Table 2) By using the atomic coordinates obtained by X-ray crystallography of FMDV O BFS, six mutations which are clustered the position occupied by the G-H loop of VP1 fixed in the capsid of ... Fujian/CHA/5/99) (A) The 93-102 amino acid deletions harbored in 3A of the porcinophilic phenotype of O/YUN/TAW/97 (B) The highly variable C-terminus was predicted probably due to the conformation of three-dimensional ... During 1997-2002, outbreaks of FMD caused by FMDV serotype O, occurred in the countries and districts of East Asia (EA) and the Far East [11] The O/ YUN/TAW/97 strain, a member of the Cathay topotype,...

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Báo cáo y học: " The establishment and characterization of the first canine hepatocellular carcinoma cell line, which resembles human oncogenic expression patterns" ppsx

Ngày tải lên : 13/08/2014, 13:20

... neoplastic nature of the cells can be deduced from the pleiomorphism of the cells, the number of sometimes bizarre mitotic figures The simultaneous presence of CK7 and HepPar1 positive cells is also ... with the neoplastic nature of the cells [11], and suggests the presence of both fully differentiated hepatocytes and progenitor cells in the cHCC cell culture The hepatocytic nature of the cHCC cell ... Note: The activity of the enzymes is given as a percentage compared to the activity of proteins in HepG2 We have described the establishment and characterization of a canine hepatocyte tumor cell...

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genetic analysis and visual characterization of the relationship between the tubulin cytoskeleton and the plant cell wall

Ngày tải lên : 13/11/2014, 09:37

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The characterization of soluble t cell receptors specific for the parasite toxoplasma gondii

Ngày tải lên : 09/09/2015, 11:31

... light chain of the BCR Thus, each light chain could either be of the κ or λ subtype On the other hand, the variable regions of the heavy chain are encoded by the recombination of the V, J and ... Thus, each BCR can theoretically bind to two target antigens The large diversity in the binding regions of the BCR is generated primarily by the recombination of genes encoding for the variable regions ... selection of cells for transfer to maximize the full therapeutic potential of this approach 20 1.6 TCR therapeutics: Soluble TCRs Another arm of T cell therapeutics would be soluble TCRs They can be...

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Characterization of the microbial community in the anaerobic/oxic/anoxic process combine

Ngày tải lên : 05/09/2013, 09:38

... members of the Bacteroidetes (A), Betaproteobacteria (B) , and Alphaproteobacteria (C) The root of the tree was determined by using the 16S rRNA gene of Methanosarcina mazei (AB065295) as the ... of Determinative Bacteriology (1994), the genus Beijerinckia is not denitrifying bacteria but PHB accumulating bacteria, and the genera Aminobacter, Hyphomicrobium, and Paracoccus have the ability ... divisions and subdivisions are shown in Table Most clone sequences were affiliated with the Bacteroidetes (33 clones belonging 23 OTUs), followed by the Betaproteobacteria (30 clones belonging to...

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Characterization of the Carbon Stable Isotope Ratio and Fatty Acid Structure of Zostera japonica in Coastal Areas

Ngày tải lên : 05/09/2013, 10:17

... at the center of the meadows, and then the samples were frozen immediately upon reaching the laboratory In September 2008, Z marina were collected from the sublittoral zone in the same area The ... the deciduous effect of the respective parts of Z japonica The δ13C in the roots and rhizomes seems to be larger than the δ13C in the leaves and stems For the same species of seagrass, when the ... those of Z marina This might be because Z japonica uses sufficient quantities of carbon for photosynthesis However, it is debatable whether other seagrasses as Z noltii have the same trend of δ13C...

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Preparation and characterization of the PVDF-based composite membrane for direct methanol fuel cells

Ngày tải lên : 05/09/2013, 16:11

... of the PVDF-SPS membrane: (a) C1s, (b) O1s, (c) S2p 3.4 Morphology of the PVDF-SPS composite membrane The morphologies of the pristine PVDF membrane, the PVDF-PS membrane and the PVDF-SPS membrane ... can be seen that the polymerization of styrene and the blending of PS and SPS polymers have affected the surface structure of the PVDF membrane obviously The cross-section morphologies of the ... membrane The hydrophobic property of PS polymer promoted the formation of these polymer aggregates As can be seen, the thermally induced polymerization of styrene, as well as the blending of...

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