characterization and classification of stem cells
báo cáo khoa học: "Epigenomics of human embryonic stem cells and induced pluripotent stem cells: insights into pluripotency and implications for disease" potx
... acetylation of histone H4; H3K4me1/2/3, (mono-, di- and tri) methylation of lysine of histone H3; H3K27ac, acetylation of lysine 27 of histone H3; H3K27me3, trimethylation of lysine 27 of histone ... Figure The combination of stem cell models and epigenomics in studies of the role of non-coding mutations in human disease Epigenomic analyses of cells derived through in vitro stem cell differentiation ... embryonic stem cell; H3K4me1, methylation of lysine of histone H3; H3K4me3, trimethylation of lysine of histone H3; H3K27ac, acetylation of lysine 27 of histone H3; H3K27me3, trimethylation of lysine...
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Vascularised bone tissue engineering endothelial progenitor cells and human mesenchymal stem cells coculture in 3d honeycomb scaffolds and the effect of bi rotational bioreactor and hypoxic microenvironment 1
... my two supervisors Prof Teoh Swee-Hin and A/Prof Jerry Chan I would like to thank them for their constant sharing of knowledge and ideas, their patience and understanding, and the many hours they ... choice of cells, scaffolds, bioreactor and microenvironment oxygen tensions Low O2 MSC + EPC Stem Cells Scaffold Bioreactor BTE Component Coculture of Honeycomb mesenchymal stem macroporous cells ... providing the bulk of the tensile yield strength and plays an important in the storage of various ions The mineral salts of bone comprises of largely of calcium and phosphorus (Downey and Siegel 2006)...
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Characterization and deactivation of sulfided red mud used as hydrogenation catalyst
... These samples, and samples of fresh unsulfided and sulfided catalyst, were characterized by nitrogen adsorption and SEM The results of textural characterization by nitrogen adsorption of the catalyst ... related to the intensity of emission of the characteristic Ka line of each element White corresponds to a high concentration of a given element, and black to the absence of this element, while ... Fig SEM-EDX maps of distribution of elements: red mud after h run time The brightness of every pixel of the EDX maps, B, can be quantified on a scale of (black) to (maximum degree of brightness)...
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Tài liệu Diagnostic Standards and Classification of Tuberculosis in Adults and Children doc
... nature of the mycobacteria endemic in different parts of the world, the use of different strains of BCG for immunization, and the use of different doses of vaccine and different schedules of immunization ... size Ϯ 20%) Other doses of tuberculin, such as and 250 TU, are remnants of the old graduated system of administration (125) and represent the smallest and largest doses of tuberculin that were ... are often seen in the upper lobes, and upper-lobe volume loss often accompanies these scars Nodules and fibrotic lesions of old healed tuberculosis have well-demarcated, sharp margins and are often...
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Tài liệu Báo cáo khoa học: Preliminary molecular characterization and crystallization of mitochondrial respiratory complex II from porcine heart ppt
... Huo et al Characterization of respiratory complex II Fig Sequencing of porcine heart mitochondrial complex II Total RNA was extracted and purified from the fresh porcine heart, and genes of four ... G250, and the four bands corresponding to subunits of porcine complex II were cut out and N-terminal sequenced by the Edman degradation method and amino acid analyzer in the Department of Biology, ... the left, and the positions of molecular weight standards are indicated on lane FEBS Journal 274 (2007) 1524–1529 ª 2007 The Authors Journal compilation ª 2007 FEBS 1525 Characterization of respiratory...
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Tài liệu Báo cáo khoa học: Physico-chemical characterization and synthesis of neuronally active a-conotoxins docx
... spectrometry and the lability of the sulfogroup in mass spectrometry analysis allows recognition of the modification, and differentiation of sulfotyrosine and phosphotyrosine [38] Characterization of the ... mechanisms of incorporation of PTMs in the biosynthesis of conopeptides Appropriate methods of analysis need to be addressed to ensure recognition of these PTMs, if present, in the course of characterization ... Amidation of C-terminus Sulfation of tyrosine Hydroxylation of proline Carboxylation of glutamic acid Cyclization of N-terminal Gln O-Glycosylation Bromination of tryptophan Isomerization of tryptophan...
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Tài liệu Báo cáo khoa học: Characterization and mode of action of an exopolygalacturonase from the hyperthermophilic bacterium Thermotoga maritima doc
... mapping On the basis of the assumptions of Hiromi [30] that the intrinsic rate of hydrolysis (kint) in the productive complex is independent of the length of the substrate, Km and Vmax were used ... Hiromi K (1970) Interpretation of dependency of rate parameters on the degree of polymerization of substrate in enzyme-catalyzed reactions Evaluation of subsite afnities of exo-enzyme Biochem Biophys ... PelB contains a few inserted b-strands (1a in Fig 2), and one large insert of 15 amino acids is present before the rst b-sheet of coil 3, which is on the edge of the pronounced substrate-binding...
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Tài liệu Báo cáo Y học: Characterization and regulation of yeast Ca2+-dependent phosphatidylethanolamine-phospholipase D activity docx
... pattern and extent of stimulation of the membrane and soluble yeast PtdEtnPLD activity by Ca2+ and their inhibition by other divalent cations is highly comparable The mechanism of action of Ca2+ ... both wild-type and spo14D cells Future identification of the PtdEtn-PLD gene and analysis of its promoter region will allow examination of its transcriptional regulation by inositol and the possible ... purification of the cytosolic PtdEtn-PLD resulted in rapid loss of activity Ó FEBS 2002 Characterization and regulation of yeast PtdEtn-PLD activity (Eur J Biochem 269) 3823 Table Effect of different...
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Characterization and Authentication of Olive and Other Vegetable Oils pptx
... Aceite de la Comunitat Valenciana Aceite de Madrid Andalucía Andalucía Andalucía Andalucía Andalucía Andalucía Andalucía Andalucía Andalucía Andalucía Andalucía Extremadura Extremadura Castilla-La ... Construction of Data Matrices and MLR Models 9.5 Prediction of OFA Concentration in VOOs Using FTIR and MLR 9.5.1 Description of FTIR Spectra and Construction of Data ... 98.5–99.5 % of oils The major part of this fraction are triacylglycerols and free fatty acids, although other fatty acid M J Lerma García, Characterization and Authentication of Olive and Other...
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Nomenclature and Classification of Congenital Heart Disease doc
... space the position of the heart and the orientation of its apex Cardiac Description the segmental anatomy of the heart the classification of the cardiac lesion the description of the cardiac lesion ... Ventricle Robert Anderson Morphologically Left Ventricle VENTRICULAR TOPOLOGY – Robert Anderson Right hand Left hand The Great Arterial Position • The viscera and atria: The types of visceroatrial ... segmental anatomy of the heart the classification of the cardiac lesion the description of the cardiac lesion Left and Right For cardiac chambers: ‘‘left’’ refers to morphologically left and ‘‘right’’...
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Báo cáo khoa học: Enzymatic toxins from snake venom: structural characterization and mechanism of catalysis ppt
... activity and toxicity of the basic subunit, is a product of divergent evolution of the catalytically active and toxic PLA2 The salt bridge between Asp48 of the PLA2 molecule and Lys60 of the acidic ... facilitate identification of the key substrates of individual SVMPs and enable a better understanding of the molecular mechanism of action of P-III SVMPs Functional role of the non-enzymatic proteins ... Interaction of the cysteine-rich domain of snake venom metalloproteinases with the A1 domain of von Willebrand factor promotes site-specific proteolysis of von Willebrand factor and inhibition of von...
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Báo cáo khoa học: Characterization and regulation of a bacterial sugar phosphatase of the haloalkanoate dehalogenase ppt
... number of phosphate containing substrates, and it has a high specificity for nucleotide monophosphates and, in particular, UMP and GMP The structure of NagD has been determined and the occurrence of ... Fig Overproduction and purification of recombinant AraL-His6 (A) Analysis of the soluble (S) and insoluble (P) protein fraction (20 lg of total protein) of induced cultures of E coli Bl21(DE3) ... for the over-expression of the recombinant proteins The cells were grown in the presence and absence of the inducer isopropyl thio-b-d-galactoside (IPTG), and soluble and insoluble fractions...
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Báo cáo khoa học: "Examining the Role of Linguistic Knowledge Sources in the Automatic Identification and Classification of Reviews" doc
... (e.g, Yu and Hatzivassiloglou (2003)) and classifying the polarity based solely on the subjective portions of the document (e.g., Pang and Lee (2004)) Motivated by the work of Koppel and Schler ... dataset Briefly, a positive review has a rating of ≥ 3.5 (out of 5) or ≥ (out of 4), whereas a negative review has a rating of ≤ (out of 5) or ≤ 1.5 (out of 4) 12 The curves are not shown due to space ... compares favorably to state -of- the-art sentiment classification systems References C Cardie, J Wiebe, T Wilson, and D Litman 2004 Lowlevel annotations and summary representations of opinions for multi-perspective...
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Báo cáo Y học: Synthesis, characterization and application of two nucleoside triphosphate analogues, GTPcNH2 and GTPcF pdf
... spectroscopic and structural studies was tested by formation of the complex between RasGTPcNH2 and the Ras-binding domain of the Ras effector protein c-Raf-1 MATERIALS AND METHODS General description of ... methanol and repeated evaporation under reduced pressure, yield of the pure GTPcNH2 was 50–60% One gram of GTP triethylammonium salt was added to a stirred solution of 2.5 mL tributylamine and 1.2 ... )5.51 )5.30 – – 0.91 0.97 – – Preparation of NMR samples and NMR spectroscopy 31 P and 19F NMR spectra of free nucleotides were recorded in aqueous solution of 90%/10% H2O/D2O Typically the lyophilized...
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Báo cáo khoa học: Mycobacterium tuberculosis ClpC1 Characterization and role of the N-terminal domain in its function ppt
... ClpC1 and its deletion mutants (A) SDS ⁄ PAGE of purified full-length ClpC1 and deletion mutants, ClpC1D1 and ClpC1D2 (B) Full-length ClpC1 and deletion mutants, ClpC1D1 and ClpC1D2; the first and ... in its pathogenesis and an understanding of 6150 their mode of action could be useful in exploring them as drug targets Results Figure shows the sequence and putative domains of M tuberculosis ... interest to understand the mode of action of these proteins in M tuberculosis In this study, we functionally characterized the ClpC1 protein of M tuberculosis, and investigated the role of its N-terminal...
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Báo cáo khoa học: Cloning, characterization and localization of a novel basic peroxidase gene from Catharanthus roseus potx
... cDNA, and lanes 1, 3, and show the same using genomic DNA of C roseus Primer pairs were: #GSP-4 and #PFLF1 (lanes and 2); #GSP-2 and #GSP-4 (lanes and 4); #GSP-2 and #PFLR-1 (lanes and 6); and ... #PFLF-1 and #PFLR-1 (lanes and 8) (B) Schematic organization of the CrPrx gene The asterisk indicates the position of the codon encoding the first amino acid of the mature protein, and the regions of ... leaf epidermal cells and (F) xylem cells of transiently transformed N tabacum with CrPrx–GUS–GFP In epidermal and stomatal guard cells, CrPrx–GFP is restricted to the cell wall and associated...
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Báo cáo khoa học: "Comparison and Classification of Dialects" doc
... Joseph Kruskal 1983 An overview of sequence comparison In David Sankoff and Joseph Kruskal, editors, Time Warps, String Edits and Macromolecules: The Theory and Practice of Sequence Comparison, pages ... dialectology in Irish Gaelic In Proc of the European ACL, pages 60-67, Dublin -John Nerbonne and Wilbert Heeringa 1999 Computational comparison and classification of dialects Zeitschrift fiir Dialektologie ... Blancquaert and W P @ e 1925-1982 Reeks Nederlandse Dialectatlassen De Sikkel, Antwerpen Cor Hoppenbrouwers and Geer Hoppenbrouwers 1988 De featurefrequentiemethode en de classificatie van nederlandse...
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Production, purification, characterization, and applications of lipases pot
... focussed particularly on structural characterization, elucidation of mechanism of action, kinetics, sequencing and cloning of lipase genes, and general characterization of performance (Alberghina et ... processing, the synthesis of fine chemicals and pharmaceuticals, paper manufacture, and production of cosmetics, and pharmaceuticals (Rubin and Dennis, 1997a,b; Kazlauskas and Bornscheuer, 1998) ... synthesis of lipid membranes and prostaglandins Microbial lipases are used to obtain PUFAs from animal and plant lipids such as menhaden oil, tuna oil, and borage oil Free PUFAs and their mono- and...
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Báo cáo khoa học: Helicobacter pylori single-stranded DNA binding protein – functional characterization and modulation of H. pylori DnaB helicase activity pptx
... indicates the position of the ssDNA in the absence of SSB protein and the subsequent retardation of the band with an increasing amount of SSB is also shown *Position of the double-stranded control DNA ... Effect of HpSSB on ATPase activity of HpDnaB in the presence of ssDNA The release of radiolabeled Pi from (c-32P)ATP was monitored in the absence and presence of different concentrations of HpSSB ... Wt and DC20 SSB protein in varying concentrations (0, 0.45, 0.9, 1.8, 2.7 and 3.6 lg, respectively) with 300 ng of M13mp18 single-stranded circular DNA and ⁄ or 300 ng of pUC18 double-stranded...
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Báo cáo khoa học: Transfection with 4-hydroxynonenal-metabolizing glutathione S-transferase isozymes leads to phenotypic transformation and immortalization of adherent cells pdf
... B-3 and CCL-75 cells ranged from 40% to 70% To determine the effect of wildtype and mutant forms of GST on injected cells, all surviving HLE B-3 and CCL-75 cells were scored at 24 and 48 h and ... left hand margins of (A) and (B) Lanes 1, 2, and in both panels represent extracts of HLE B-3 cells transfected with wild-type, empty vector and hGSTA4, respectively (C) For comparison of expression ... designated C4, D7 and The anchorage-independent growth of phenotypically transformed cells was confirmed by assay of soft agar colonies [26] Clone C4 cells grew into colonies within weeks of plating,...
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