chaperonin mediated folding of viral proteins
Báo cáo khoa học: The association of viral proteins with host cell dynein components during virus infection pdf
... Retroviridae Viral UL34 Viral UL9 (helicase) Viral UL35 (VP26) Viral U19 Viral p54 Viral phosphoprotein (P) Viral phosphoprotein (P) Viral minor capsid protein L2 Viral probable protein E4 Probably viral ... traffic of viral particles towards the nucleus but also by the fact that microinjection of anti-dynein antibodies reduced the nuclear accumulation of viral capsids and immunoprecipitation of dynein ... fluorescent protein (GFP) tagged viral proteins has also demonstrated the retrograde advance of viruses towards the cell Fig Viral retrograde transport model Both the entry of the viral particle through...
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Báo cáo hóa học: " Predicting the subcellular localization of viral proteins within a mammalian host cell" doc
... understanding of the function of these proteins, of their role in the cell and of the consequences of host-pathogen interactions They might also serve to devise more efficient methods of treatment ... degree of interaction of these enveloped viruses with cellular membranes for functions that include assembly of viral particles and budding of the virus [26], and thus the need for insertion of a ... the inhibition of apoptosis [7] GPI anchors, which allow the attachment of proteins to the extracellular leaflet of the plasma membrane, are also predicted to be used by these viral proteins, to...
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Báo cáo y học: " Increased production of viral proteins by a 3''''-LTR-deleted infectious clone of human T-cell leukemia virus type 1" ppsx
... deletions of the 3' LTR of different lengths and the corresponding viral expression patterns Various Various molecular clones with deletions of the 3' LTR of different lengths and the corresponding viral ... expression patterns (A) Diagram of the molecular clones showing the deletion series of the 3' LTR and the deletion of the reverse TATA box of the 3' LTR (B) The amount of p19 gag in the pooled concentrated ... downstream from the beginning of the 3' LTR, which constitutes the C-terminal portion of the tax downstream sequence, might be involved in the inhibition of the replication of HTLV-1 genes in infectious...
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Tài liệu Báo cáo khoa học: Minor capsid proteins of mouse polyomavirus are inducers of apoptosis when produced individually but are only moderate contributors to cell death during the late phase of viral infection ppt
... fused proteins (Fig S1B) Intracellular localization of the wt minor proteins and their fusion variants Distribution of the fused minor capsid proteins was followed through the analysis of confocal ... Recently, viroporins of RNA viruses (such as 6K protein of Sindbis virus, E protein of mouse hepatitis virus, M2 protein from influenza A, 2B and 3A proteins of poliovirus, or p7 and NS4A of hepatitis ... levels of apoptotic markers during the late stages of MPyV infection However, comparison of the levels of apoptotic markers and cytotoxicity during the replication of wt MPyV, and replication of...
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Báo cáo khoa học: Thermodynamic stability and folding of proteins from hyperthermophilic organisms doc
... hyperthermostable: (a) co -chaperonin proteins, (b) nonheme iron proteins, (c) DNA-binding proteins, as well as (d) a few other proteins Although the number of characterized hyperthermostable proteins is rather ... (TM of 50 °C, pH 7; DGU of 11 kJÆmol)1, pH 7, 20 °C) [44] This difference in thermodynamic stability correlates with two orders of magnitude slower unfolding of TmCsp as compared to unfolding of ... entropy-driven assembly of cochaperonin protein 10 Biophys J 89, 3332–3336 Luke K & Wittung-Stafshede P (2006) Folding and assembly pathways of co -chaperonin proteins 10: origin of thermostability...
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Báo cáo khoa học: DNA mediated disassembly of hRad51 and hRad52 proteins and recruitment of hRad51 to ssDNA by hRad52 pot
... supernatant fraction of the assay Both of the assays suggested that addition of ssDNA facilitates significant level of disaggregation in hRad51 To reconfirm the DNA mediated disaggregation of hRad51, we ... effect of DNA on hydrodynamic radius (Rh) of hRad51 hRad51 (1 lM) was incubated with 0, 1, 3, and lM of oligo PUC+ (in the absence of any nucleotide cofactor) followed by the measurement of hydrodynamic ... disappearance of small ones To assess the role of hRad52 on hRad51 binding, we performed gel shift analyses of complexes at a limiting amount of hRad51 (1 lm) in the presence of increasing levels of hRad52...
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Báo cáo khoa học: A novel plant protein disulfide isomerase family homologous to animal P5 – molecular cloning and characterization as a functional protein for folding of soybean seed-storage proteins docx
... involvement of the other PDI proteins in the folding of storage proteins remains unclear In this study, we isolated cDNA clones and genomic sequences encoding a soybean group V gene of the PDI ... (supplementary Fig S4) Nucleotide sequence of the ORF of the GmPDIM gene was identical to that of the cDNA Comparison of the soybean genomic sequence of GmPDIM with those of A thaliana (AGI number At1g04980 ... sequence of branch points of plants (YTNAN) upstream of the 3¢ splice site [24] We next analyzed the promoter region of GmPDIM, 2340 bp upstream of the translational initiation codon ATG A search of...
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Báo cáo sinh học: "Molecular and cellular correlates of the CIITA-mediated inhibition of HTLV-2 Tax-2 transactivator function resulting in loss of viral replication" pot
... al.: Molecular and cellular correlates of the CIITA -mediated inhibition of HTLV-2 Tax-2 transactivator function resulting in loss of viral replication Journal of Translational Medicine 2011 9:106 ... of the CIITA mediated inhibition of Tax-2 We found that both CIITA and NF-Y interact in vivo with Tax-2 We identified both an N-terminal and a Cterminal region of CIITA interacting with the viral ... the basis of the functional inactivation of Tax-2 leading to the inhibition of HTLV-2 retrovirus replication Further studies of subcellular localization unveiled the co-localization of Tax-2 and...
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Báo cáo sinh học: " CODEHOP-mediated PCR – A powerful technique for the identification and characterization of viral genomes" pptx
... provided The use of the CODEHOP methodology for the analysis of larger regions of viral genomes is presented along with the general application of this technology for the identification of viral species ... genomes Often the linear order of genes within the genomes of related viruses is maintained Thus, the spacing and orientation of specific genes can be predicted in the genomes of Page 15 of 24 ... Figure KSHV of a region12 the divergent locus B of a complete sequence CODEHOP strategy to determine themacaque homolog ofof CODEHOP strategy to determine the complete sequence of a region of the...
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Báo cáo hóa học: " A comparative analysis of viral matrix proteins using disorder predictors" docx
... Patterns of EIAV Are Closer to Those of Influenza than of disorder patterns of HIV/SIV Analysis of Figure reveals that the pattern of the predicted disorder in EIAV matrix protein is closer to that of ... behavior of the matrix proteins [3] Conclusion Matrix Disorder of Retroviruses Varies with Nature of the Virus A peculiar finding of this paper is the pattern of predicted disorder of EIAV p15 ... disorder-disorder interactions Comparison of the disorder and B-factor profiles of the HIV-1 and SIVmac p17 proteins allows extrapolation to be made of the potential modes of inter-subunit interactions in...
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o cáo hóa học:" Molecular and cellular correlates of the CIITA-mediated inhibition of HTLV-2 Tax-2 transactivator function resulting in loss of viral replication" potx
... al.: Molecular and cellular correlates of the CIITA -mediated inhibition of HTLV-2 Tax-2 transactivator function resulting in loss of viral replication Journal of Translational Medicine 2011 9:106 ... of the CIITA mediated inhibition of Tax-2 We found that both CIITA and NF-Y interact in vivo with Tax-2 We identified both an N-terminal and a Cterminal region of CIITA interacting with the viral ... the basis of the functional inactivation of Tax-2 leading to the inhibition of HTLV-2 retrovirus replication Further studies of subcellular localization unveiled the co-localization of Tax-2 and...
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báo cáo khoa học: " Truncated forms of viral VP2 proteins fused to EGFP assemble into fluorescent parvovirus-like particles" doc
... curves of of fluorescent recombinant proteins The effect of trypsin on the fluorescence autocorrelation curves of the fluorescent recombinant proteins Normalized autocorrelation curves of EGFP-VP2 ... the structure of the viral protein in order to allow capsid formation In this study, deletions of 14 amino acids from the N-terminal region of VP2 proteins without any insertions of amino acids, ... Figure Immunoblot analyses of the recombinant proteins Immunoblot analyses of the recombinant proteins Baculovirus infected Sf9 cells (A and B) expressing the fusion proteins EGFP-VP2, EGFP-VP2-14,...
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Báo cáo y học: "Common principles and intermediates of viral protein-mediated fusion: the HIV-1 paradigm" pps
... functional domains of viral fusion proteins from immune surveillance and/or ensure the release of the viral genome at preferred cellular sites A generalized mechanism of viral fusion In spite of structural ... levels of infection that rely on successful completion of viral replication steps downstream of fusion may underestimate the efficacy of fusion [33,34] Novel techniques monitoring the delivery of viral ... of activation While the structures of ectodomains (or their core fragments) have been solved for several viral proteins, information regarding intermediate conformations of fulllength viral proteins...
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Báo cáo y học: "The control of viral infection by tripartite motif proteins and cyclophilin A" ppt
... Similarity between the structures of retroviral capsids Similarity between the structures of retroviral capsids Superimposition of the structures of the N terminal domains of HIV-1 (Red) and MLV (blue) ... AY523867 Figure Similarity between the sequences of retroviral capsids Similarity between the sequences of retroviral capsids Alignment of primate lentiviral capsid protein sequences demonstrates ... influencing the activity of another antiviral TRIM protein For example, expression of TRIM34 can reduce the antiviral activity of TRIM5 presumably via heteromutimerisation mediated via the coiled...
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Báo cáo sinh học: " Anti-metastatic effects of viral and non-viral mediated Nk4 delivery to tumours" doc
... result of DNA sequence, at least at the level of plasmidmediated expression Nevertheless, it cannot be ruled out that elements of the AAV vector were counteracting the therapeutic efficacy of Nk4 ... elicits no cell -mediated immune response To assess if prolonged and increased levels of expression at later time points would improve therapeutic responses, AAV2 mediated delivery of the Nk4 cassette ... generated by cloning of the Nco1, Xba1 fragment of pGL3 (Promega) containing the firefly luciferase gene, by blunt end ligation in the EcoRI, Xba1 region downstream of the CMV promoter of AAV-MCS cloning...
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FOLDING OF PROTEINS IN PRESCULPTED FREE ENERGY LANDSCAPE
... number of minima that correspond to a menu of folds The role of amino acid sequence is to choose the structures from such a menu of predetermined folds In this study, we investigate how the folding ... an energy of −0.7ǫ Additionally, a cooperative energy of −0.3ǫ is given for each pair of hydrogen bonds that are formed by pairs of consecutive amino acids in the sequence Two models of hydrophobic ... the free energy landscape so that the number of possible ground states is drastically reduced comparing to that of conventional polymers Folding of proteins in presculpted free energy landscape...
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Intein mediated biotinylation of proteins and its application in protein microarray
... of the target protein indicates the intein cleavage site 23 3.2 Intein -Mediated Biotinylation of three model proteins 3.2.1 Cloning of target genes into pTYB1 expression vector In the proof -of- concept ... biotinylation of yeast proteins 43 3.6.1 Cloning of yeast gene into pTYB1 expression vector 43 3.6.2 Expression, purification & biotinylation of yeast proteins 45 3.7 In vivo biotinylation of proteins 48 ... studies of proteins both in vitro and in vivo v List of Tables Table Page The influence of C-terminal residues on the in vivo cleavage of EGFP-intein and on-column cleavage/biotinylation of EGFP...
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Intein mediated generation of n terminal cysteine proteins and their applications in live cell bioimaging and protein microarray
... the integration of various disciplines in science Consequently, the role of chemistry has never been so profound in the study of biological processes By combining the strengths of recombinant ... incorporation of molecular entities with unique chemical functionalities in proteins has many potential applications in chemical and biological studies of proteins In this study, we present an intein -mediated ... interactions of proteins inside living cells is critical for a better understanding of cellular mechanisms and functions Traditionally this has been done by in vitro labeling of proteins with...
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Use of Cyanobacterial Proteins to Engineer New Crops
... biofarming is a major goal of plant biotechnology Direct transfer of plant genes has resulted in new varieties with improved properties However, this approach is limited by the genetic stock of ... the various existing Use of Cyanobacterial Proteins 67 Fig 4.1 Summary of current advances in plant biotechnology using cyanobacterial genes Gene mining on cyanobacteria offers a promising opportunity ... phytochrome reaction spectra Production of biodegradable polymers (Section 4.5) Phytochrome perception (Section 4.6) (synthesis of PCB) Biofarming Production of cyanophycin Pigment manipulation (Section...
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