... describe the linkage map ofthehoneybee and its anchorage on the sequenced-based physical map We also draw out the emerging properties ofthehoneybee meioses and compare them to those of some ... honeygenotyped honey bee, forcontaining sequencesize andinthename,map of colA PDFandKosambithe a microsatellite-basedthethe scaffolds ,the Additionallinesscaffolds ,the4 .0),areof theThecoordinates markers, the ... physical unit of 22 cM/Mb, the average for the whole genome ofthehoneybee is 20 times greater than that of humans [42], 1.0 cM/Mb As the females ofthe two species have about the same genetic...
... quantification of a virus genome and antigenome in vivo using thehoneybee as a model This permitted the specific identification of an important honeybee virus (DWV) replication sites in thehoneybee ... Bee Culture 2000, 128:1-15 Allen M, Ball B: The incidence and world distribution ofhoneybee viruses Bee World 1996, 77:141-162 Ellis JD, Munn PA: The worldwide health status ofhoney bees Bee ... transmission of DWV and suggested that the lining ofthe digestive tract was likely the primary site ofthe DWV infection via food-borne transmission, with the virus spreading to secondary sites from the...
... abolished the internalization ofthe GFP-fused Tat protein but did not alter the uptake ofthe Tat CPP These biochemical evidences con®rmed a pathway for the entry ofthe Tat peptide unrelated to the ... rightward shift ofthe signal was observed signifying a reduction ofthe uptake ofthe Tat peptide at low temperature Likewise, a threefold reduction ofthe uptake at °C has been reported for the Antennapedia ... concentration of lgámL)1 in the presence of 100 lM chloroquine in the cell culture medium For FACS analysis, the concentration ofthe recombinant protein was increased to lgámL)1 The internalization of the...
... Although the effect of ecdysone on neurons ofthe central nervous system has been extensively studied in Drosophila and Manduca, little information is available on the role of EcR expression in thebrain ... pattern of expression RpL32 expression did not differ between the two parts ofthebrain Cell-specific expression of EcR isoforms as revealed by in situ hybridization The spatial specificity of EcR-A ... distribution of gene expression (C) RT-PCR analysis of EcR expression using RNA from the anterior (A) and posterior (P) part ofthe brains The number of PCR cycles is indicated above the panel 3862...
... likely in the CIITA-NF-YB molecular complex, is at the basis ofthe functional inactivation of Tax-2 leading to the inhibition of HTLV-2 retrovirus replication Further studies of subcellular localization ... distribution of Tax-2 molecules was analyzed in the presence and in the absence of CIITA In the absence of CIITA, Tax-2 localizes both and in the cytoplasm and in the nucleus of 293T cells often with ... region of CIITA Furthermore, these results strongly suggest that interaction between the N-terminal, but not the C-terminal, part of CIITA, and Tax-2 is responsible ofthe biological effect of CIITA...
... likely in the CIITA-NF-YB molecular complex, is at the basis ofthe functional inactivation of Tax-2 leading to the inhibition of HTLV-2 retrovirus replication Further studies of subcellular localization ... distribution of Tax-2 molecules was analyzed in the presence and in the absence of CIITA In the absence of CIITA, Tax-2 localizes both and in the cytoplasm and in the nucleus of 293T cells often with ... region of CIITA Furthermore, these results strongly suggest that interaction between the N-terminal, but not the C-terminal, part of CIITA, and Tax-2 is responsible ofthe biological effect of CIITA...
... deeper into the latest science ofthe modern middle-aged brain, I found not bad news but good As it turns out, thebrain in middle age has another story to tell that’s quite the opposite ofthe one ... our brains, they are neither as uniform nor as drastic as we feared Indeed, even the long-held view that our brains lose millions ofbrain cells through the years has now been discounted Using brain ... people who knew what they were doing These were people, also in the thick of middle age, who, despite not remembering the name ofthe restaurant they just ate in or the book they just read, were...
... every bee experiment at each distance Therefore, we suggest that the lack of enrichment for these same GO slim terms in thebee experiment may reflect the lack of a complete gene list in honey bee, ... support ofthe postulated role of miRNAs in regulating the alternative developmental trajectories associated with caste differentiation, we examined the functional role ofhoneybee official ... in honey bees Some of these novel miRNAs appear to have been overlooked in analyses ofthe well-studied insect Drosophila melanogaster and other genomes Results Computational identification of...
... Franklin Neither this thesis, nor the man I am today, would be possible without the heroic effort of Professor Too Heng-Phon, whose philosophy of mentoring is a true embodiment ofthe quote Professor ... this thesis is my original work and it has been written by me in its entirety I have duly acknowledged all the sources of information which have been used in the thesis This thesis has also not been ... provided conflicting results ofthe physiological role of this pathway in the maintenance of adult neurons No obvious differences in the morphology or biochemical properties ofthe dopaminergic nigrostriatal...
... disruption ofthe association of these proteins could represent a potential therapeutic strategy for the treatment of AD Here, we used GST fusion proteins of Fyn-SH2 and FynSH3 to further investigate the ... whether the tyrosine phosphorylation status of tau also affects the binding of tau to Fyn-SH3, CHO cells were cotransfected with Fyn together with either wild-type or the YF mutant forms of tau ... The SH3 domain of Fyn binds to proline-rich motifs within the sequence PXXP in interacting proteins Seven such motifs are present in the proline-rich domain ofthe longest isoform of tau in the...
... addition of DA (Fig 5) Discussion Degeneration ofthe dopaminergic neurons ofthe substantia nigra pars compacta is responsible for the major motor symptoms of PD The neurotransmitter DA has been ... counter Values are the mean of six replicates The specific uptake was determined by subtracting the uptake counts in the absence of inhibitor from the uptake counts in the presence of cocaine or S-citalopram, ... total concentration of DA and the y axis shows the DA uptake after 10 The y axis demonstrates specific uptake, defined as total uptake subtracted the uptake in the presence ofthe DAT inhibitor cocaine...
... roles of GIF within thebrain Further studies are required to determine the function of GIF within the normal, injured and neurodegenerative brain, and to establish the therapeutic potential ofthe ... brain injury or neurodegenerative disease Synthesis and secretion of GIF in thebrainCellular and temporal localization of GIF synthesis Whilst the MT1 and MT2 isoforms are ubiquitously synthesized ... conditions [22] In the traumatically injured brain, however, the synthesis of MT1 and MT2, as well as the synthesis of GIF, are up-regulated by reactive astrocytes in the vicinity ofthe lesion [23]...
... through the extracellular matrix with the help of matrix metalloproteinase [82] The consequent event ofbrain macrophages is one ofthe most important defense mechanisms used by thebrain However, ... and barriergenesis fers according to the size ofthe vessels In the brain, pial arteries invade thebrain parenchyma and reduce the width of arterioles, then form deep branches that become small ... In particular, the concept of a ‘neurovascular unit’ that encompasses neurons, vessels and other types of cells located at their interface has become one ofthe main themes ofbrain science [43,44]...
... Physiology and pathophysiology ofthe SREBP family H Shimano at G1 [19] In particular, p21 is a direct target of SREBP [20] The role of SREBP-1a in the regulation of cell growth and the cell cycle ... H Shimano Physiology and pathophysiology ofthe SREBP family SREBP-1c and lipogenesis The SREBP family consists of three isoforms: SREBP1a, SREBP-1c, and SREBP-2 Each isoform has a different ... resistance [48] The exact roles of SREBP-1c ⁄ ADD1 are not yet fully defined SREBP and parasympathetic function in heart Parasympathetic stimulation ofthe heart involves activation of GIRK1 ⁄ 4,...
... [17,18] In several ofthe ARTs, the replacement of this second glutamate abolishes the ability of these transferases to use arginine as acceptors, thus further supporting the hypothesis that this ... Despite the low similarity at the level of their amino-acid sequences, there are common structural features that characterize this family of mammalian ARTs [15,16] The catalytic domain of these ... needs to be clarified is the occurrence ofthe extracellular NAD+ that is required to sustain the ADP-ribosylation reaction The steadystate concentration of NAD+ in the serum of healthy individuals...
... world!" "Snakes ofthe Santo!" Friday gasped, the whites of his eyes showing all around "Then we must be on an asteroid!" They were From the far side ofthe dome ahead of them the asteroid stretched ... acceptance ofthe astounding fact; he was too busy to make comment He was observing the buildings, the nature of them, the exits from the dome, how they could best be reached T HEY stood on the roof of ... In the middle of that night, when the pleasures of Porno were in full stride, there emerged suddenly, from one ofthe dark, crooked byways that angled off the Street ofthe Sailors, a squad of...
... investigated thecellular localization of GFPtagged versions of these proteins, as well as their ability to support growth Besides clarifying the relative importance of different regions ofthe Lsm1 ... (Fig 1B) [32] The Lsm1 and Lsm8 polypeptides are most similar in the regions ofthe Sm1 and Sm2 motifs These motifs form the Sm-fold, the hallmark ofthe Sm-like proteins, consisting of a five-stranded ... for the localization and function of these complexes In budding yeast, only one form ofthe homologous Sm complex exists; it forms the core of non-U6 spliceosomal snRNPs and accumulates in the...
... level over the time period ofthe experiments as conducted here As shown below, alterations in the levels ofthe differentially glycosylated forms of GC-C regulate thecellular response to the ST-peptide, ... thought they were able to bind the ST peptide (Fig 2) Therefore, there appeared to be a correlation between the presence ofthe 145 kDa form of GC-C, which represents the mature glycosylated form of ... amount of 130 kDa protein was localized on the surface ofThe studies described in this report suggest that regulation ofthe glycosylation of GC-C can act as a means of controlling the ability of...
... 77 FIVE The Mommy Brain 95 SIX Emotion: The Feeling Brain 117 SEVEN The Mature Female Brain 135 EPILOGUE The Future ofthe Female Brain 159 vii C o n t e n ts APPENDIX ONE: The Female Brain and ... displays of status They often express agreement with a partner’s suggestions And when they have ideas of their own, they’ll put them in the form of questions, such as “I’ll be the teacher, okay?” Their ... created The boys pushed the girls around, refused to take turns, and would ignore a girl’s request to stop or give the toy back By the end ofthe morning, Leila had retreated to the other end of the...