cell cycle regulated transcription in yeast

Báo cáo y học: "BRCA1 May Modulate Neuronal Cell Cycle Re-Entry in Alzheimer Disease"

... the cell, working with other factors to halt the cell cycle and mediate DNA repair. Interestingly, a much higher proportion of NFT were labeled in AD cases than in control cases. These findings ... neuronal cell cycle are un-clear. In this regard, given that a dysregulated cell cycle is a characteristic of cancer, we speculated that altera-tions in oncogenic proteins may play a role in neurodegeneration. ... in all cases of Alzheimer disease. These findings not only further define the cell cycle reentry phenotype in Alzheimer disease but also indicate that the neurofibrillary tangles which define...

Báo cáo hóa học: " Research Article Computational Methods for Estimation of Cell Cycle Phase Distributions of Yeast Cells" docx

Danh mục: Báo cáo khoa học

Tài liệu Báo cáo khoa học: The central role of CDE/CHR promoter elements in the regulation of cell cycle-dependent gene transcription pdf

Danh mục: Báo cáo khoa học

... gene transcription [3].Interest in control mechanisms of cyclin B1 and cyclinB2 cell cycle- dependent transcription began early[3,30–33]. When investigating the regulation of humancyclin B1 transcription, ... alterations during the cell cycle, making it unlikely that protein binding to theCDE ⁄ CHR is affected by change in DNA methylationduring different phases of cell division [34].Another cell cycle gene, ... Promoterbinding factors regulating cyclin B transcription in thesea urchin embryo. DNA Cell Biol 14, 869–881.34 Tscho¨p K & Engeland K (2007) Cell cycle- dependent transcription of cyclin B2...

Tài liệu Báo cáo khoa học: Protein kinase CK2 activates the atypical Rio1p kinase and promotes its cell-cycle phase-dependent degradation in yeast pdf

Danh mục: Báo cáo khoa học

... aprotinin, leupeptin and pepstatin, twice in kinasebuffer, and used for in vitro kinase assays. In vitro kinase assaysRecombinant human protein kinase CK2 holoenzyme(0.5 U, corresponding to ... 2A,C; in C, only a faint signal wasdetected).The above results indicated that the C-terminus ofRio1p might play a role in the interaction with Cka2p in yeast cells. To determine domains of ... G1phase [11].Keywordsatypical protein kinase; casein kinase 2substrate; cell- cycle phase-dependentdegradation; protein–protein interactions;Rio1 protein kinase21CorrespondenceM. AngermayrE-mail:...

Báo cáo khoa học: 3 Cdt1 and geminin are down-regulated upon cell cycle exit and are over-expressed in cancer-derived cell lines potx

Danh mục: Báo cáo khoa học

... of the geminin proteindiffers between cell line s, r esulting in gross differences in relative amounts of Cdt1 and its i nhibitor in different cell lines. I t would b e interesting to investigate ... during the cell cycle in human cells, accumulating only du ring the G1 ph ase,when licensing is legitimate [13]. When cells exit the cell cycle, licensing in lost, a nd is est ablished again ... full-length Cdt1 and geminin (HisT7-geminin and His-geminin, amount of recombinant protein run in each lane in ng is indicated) and immunoblotted with anti-Cdt1 and anti-geminin Ig. See Materials...

Cell Cycle Regulation in Mammalian Germ Cells

Danh mục: Môi trường

... proteins act as mediators of various cell cycle regulators, in- cluding Cdk activity. The CKS (Cdc28 kinase subunit) protein family isthe mammalian homolog of the yeast Cdk binding proteins Cks1 ... Chaperone protein involved in protein folding, Dix et al. 199670 kDa protein 2 (HSP70-2) leads to spermatogenic arrest at metaphase stage in knockouts. Cell Cycle Regulation in Mammalian Germ Cells 351Table ... However, understanding cell cycle regulation in mammalian germcells has considerably lagged behind that of the mitotic cell cycle. Challengesto investigations of meiosis in mammals have included the...

The Retinoblastoma Gene Family in Cell Cycle Regulation and Suppression of Tumorigenesis

Danh mục: Môi trường

... variant containing the DNA binding motif but not the pRb-binding ortransactivation domain or introduction of a competitor plasmid containingmultiple E2F binding sites, preventing the binding of E2F ... Thepocket-domain is responsible for binding to proteins containing LXCXE motifs while thepocket-domain and the C-domain are involved in binding E2F proteins. Mdm2 (as wellas c-Abl) binds to the C-domain. ... overlapping roles in controlling cell cycle exit upon growth-inhibiting signals. Only ablation of all pocket proteins fullyalleviated a cell cycle arrest upon serum withdrawal, cell- cell contact inhi-bition,...

The ubiquitin-proteasome pathway in cell cycle control

Danh mục: Môi trường

... for linking cell cycle regulation to internal and external signaling pathways via regulated protein kinaseactivities.1IntroductionThe importance of ubiquitin-mediated proteolysis for cell cycle ... remain to be elucidated (Kominami et al.1998; Suzuki et al. 2000). Interestingly, there are three classes of related F-box proteins in both yeast and mammalian cells that are involved in cell cycle control. ... the spindle assembly checkpoint, which will be discussed in greaterdetail below, maintains Cdc20 in an inactive state until bipolar attachment ofThe ubiquitin-proteasome pathway in cell cycle...

Báo cáo khoa học: Site-speciﬁc phosphorylation of MCM4 during the cell cycle in mammalian cells pot

Danh mục: Báo cáo khoa học

... HeLa cell cycle in a site-speciﬁc manner.Cyclin-dependent protein kinase is involved in the phosphorylation of MCM4To determine which kinase is involved in the phos-phorylation of MCM4 in phases ... ofDNA in a single cell cycle. Inactivation of CDK1 leadsto re-replication of DNA in eukaryotic cells includinghuman cells [7]. Targets of the kinase in the regulationof DNA replication include ... proteins onlicensed chromatin in the G1phase was detached fromchromatin. Phosphorylation of MCM4 and other fac-tors may be involved in stronger binding of MCM4protein to chromatin in the...

Báo cáo khoa học: A transcription factor of lipid synthesis, sterol regulatory element-binding protein (SREBP)-1a causes G1 cell-cycle arrest after accumulation of cyclin-dependent kinase (cdk) inhibitors pdf

Danh mục: Báo cáo khoa học

... protein in CHO-BP1a cells, p21 and p27 proteins were markedlyinduced, as shown by immunoblot analysis (Fig. 4B). In accordance with the induction of these cdk inhibi-tors, SREBP-1a-expressing cells ... activation of acyclin E–cdk2 complex during the G1phase of thehuman cell cycle. Science 257, 1689–1694.20 Weinberg RA (1995) The retinoblastoma protein and cell cycle control. Cell 81, 323–330.21 ... (1998) Characterization of two Chinese hamsterovary cell lines expressing the COOH-terminal domainsof sterol regulatory element-binding protein (SREBP)-1. Cell Struct Funct 23 , 187–192.33...

Báo cáo Y học: Bax-induced cell death in yeast depends on mitochondrial lipid oxidation doc

Danh mục: Báo cáo khoa học

... colony-forming cells was determined as indicated in the methods section.Fig. 6. Eﬀect of fatty acid-unsaturation-inducer dioctylphtalate onh-Bax-induced cell death kinetics. WtB1cellsweregrowninacom-mercial ... measured in Yeast Nitrogen Basemedium (7.05 ± 0.05; n ¼ 3). Kinetics of Bax-induced cell death varied accordingly to the extent of Bax-inducedmitochondrial lipid oxidation. In the minimal mediumwithout ... dioctylphtalate, Bax-induced cell death kinetics wasmuch slower than in Yeast Nitrogen Base medium (Fig. 6).The addition of dioctylphtalate restored cell death kineticscomparable to experiments in Yeast...

Báo cáo khoa học: Collective behavior in gene regulation: The cell is an oscillator, the cell cycle a developmental process doc

Danh mục: Báo cáo khoa học

... trends in the Stanford yeast cell- cycle data to uncover genome-wide oscillations in expression in  4500 of the 6178gene expression proﬁles. These were typically of cell- cycle or half -cell- cycle ...  40-min cycles (Fig. 3A,C)are similar to one another in forming a bowl or conicalshape around the steady state. In other projections ofthe phenelzine-treated cells, the increase in cycle ... the epithelial cells of thegastro-intestinal tract are similar – if on different timescales. In the gastrointestinal tract of mammals the cell- cycle time of a particular cell is in the range5–10...

Báo cáo khoa học: Gene expression waves Cell cycle independent collective dynamics in cultured cells doc

Danh mục: Báo cáo khoa học

... colony as a whole.An intriguing candidate is the lectin-like proteininvolved in ﬂocculation, a cell wall protein that bindsto mannose chains on the surface of other cells toprovide the physical ... cultured yeast cells in terms of transcriptional activity led to thenotation that collective modes of whole population ofcells indeed in uence transcriptional machinery. Thissynchronization, having a ... unity indicates the non-noisy character of theextracted modes, pointing to the highly coordinatedgene expression behaviour of these cells.HeLa cells display whole genome cell cycle independent...

Báo cáo khoa học: Analysis of the contribution of changes in mRNA stability to the changes in steady-state levels of cyclin mRNA in the mammalian cell cycle doc

Danh mục: Báo cáo khoa học

... cell cycle [11–15]. Cer-tain transacting proteins such as HuR were proposedas regulators of changes in mRNA stability during the cell cycle [15]. In this context it is of interest that duringvertebrate ... elutriated cells. The cell cycle distribution of the cell fractions is shown in Fig. 4.ABCDFig. 1. Steady-state levels of cyclin mRNAs in enriched cell cycle fractions of mouse EL4 cells. Cells ... anincrease in late S and G2⁄ M phase conﬁrming previousobservations [26,27], but no cell cycle- dependentchange in stability. The cyclin E1 mRNA wasincreased in the G1phase of the cell cycle, ...