cd4 cd11b and cd4 cd11b subsets of putative myeloid dendritic cells
Báo cáo y học: "Indoleamine 2,3-dioxygenase-expressing dendritic cells are involved in the generation of CD4+CD25+ regulatory T cells in Peyer''''s patches in an orally tolerized, collagen-induced arthritis mouse model" ppt
Ngày tải lên :
09/08/2014, 10:22
... with CD4+ CD25- T cells (1 × 105 cells) obtained from Peyer's patch cells of tolerized mice in the presence or absence of CII To measure the amount of intracellular Foxp3 in CD4+ CD25+ T cells, CD4+ ... 2,3-dioxygenase-dependent induction of antigen-specific CD4+ CD25+ T cells by CD11c+ dendritic cells in tolerized mice Regulatory APCs may form a bridge between regulatory T cells and responder T cells, and this has ... in the conversion of CD4+ CD25- T cells into CD4+ CD25+ T cells (Figure 5d) Our findings show that highly purified CD4+ CD25- T cells can be converted into CD4+ CD25+ Foxp3+ T cells by DCs from tolerized...
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báo cáo hóa học:" In vitro generation of cytotoxic and regulatory T cells by fusions of human dendritic cells and hepatocellular carcinoma cells" docx
Ngày tải lên :
18/06/2014, 15:20
... differentiation and function of dendritic cells J Immunol 2005, 174:7764-7772 Li L, Li SP, Min J: Hepatoma cells inhibit the differentiation and maturation of dendritic cells and increase the production of ... cells were purified, cultured, and analyzed by 3-color flow cytometry for expression of CD4, CD25, and Foxp3 Three different populations; a) CD4+ CD25high T cells; b) CD4+ CD25low T cells; c) CD4+ CD25- ... HCC cells used for fusion expressed high levels of WT1 and HLA-ABC and low levels of CEA but not HLA-DR, CD80, CD86, and CD83 molecules (Figure 1B, 2A, and 5A) Fusions of DCs to the HCC cells...
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báo cáo khoa học: "Upregulated expression of indoleamine 2, 3-dioxygenase in CHO cells induces apoptosis of competent T cells and increases proportion of Treg cells" pptx
Ngày tải lên :
10/08/2014, 10:21
... CHO cells possessed functional activity and could metabolize tryptophan (Figure 1D) Effect of IDO+ CHO cells on CD3+T cell apoptosis After 72 h of co-culture of CD3+T cells and IDO+ CHO cells, ... specific inhibitor of IDO in co-culture of CD3+T cells and IDO+ CHO cells, the apoptosis could not be induced (only 33.1 ± 4.87% of CD3+T cells were apoptotic) (Figure 2) Page of 10 cells co-cultured ... co-culture of IDO+ CHO cells and CD3+ T cells, Foxp3 gene expression was detected in the CD3+ T cells by RT-PCR analysis CD3+T cells alone and CD3+T cells co-cultured with CHO/EGFP cells were...
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Tài liệu Báo cáo Y học: A novel meta-cleavage dioxygenase that cleaves a carboxyl-groupsubstituted 2-aminophenol Purification and characterization of 4-amino-3-hydroxybenzoate 2,3-dioxygenase from Bordetella sp. strain 10d doc
Ngày tải lên :
21/02/2014, 01:21
... 1,2,4-trihydroxybenzene and pyrogallol) in aqueous solution were always freshly prepared and used immediately Effect of various compounds on the enzyme activity The effect of metal salts, and chelating and sulfhydryl ... ± 0.10 lmol of 4-amino-3-hydroxybenzoic acid and 0.90 ± 0.08 lmol of O2 were consumed and 1.1 ± 0.02 lmol of 2,5-pyridinedicarboxylic acid was formed, which indicated a molar ratio of 4-amino-3-hydroxybenzoic ... purification All steps of the enzyme purification were carried out at 0–4 °C All centrifugations were at 20 000 g and °C for 10 A wet weight of 30 g of Bordetella sp strain 10d cells were obtained...
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Báo cáo khoa học: The role of residue Thr249 in modulating the catalytic efficiency and substrate specificity of catechol-2,3-dioxygenase from Pseudomonas stutzeri OX1 ppt
Ngày tải lên :
30/03/2014, 10:20
... basis of the structure Synthesis and characterization of 3,5-DMC and 3,6-DMC Determination of regioselectivity on 3-MC and 3,5-DMC Synthesis of 3,5-DMC and 3,6-DMC was achieved by a modification of ... of subsite 3¢ (Fig 5), and hence of the ability of the enzyme to bind 3,5-DMC and 3,6-DMC in an orientation similar to those of catechol and 3-MC The Km values of catechol and 3-MC (Table 1) show ... two residues and the edge of the substrate ring could prevent binding of 3,6-DMC and 3,5-DMC, as shown in Fig 2D,E,F Thus, the binding of 3,5-DMC and 3,6-DMC to the active site of C2,3O could...
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báo cáo hóa học:" Generation in vivo of peptide-specific cytotoxic T cells and presence of regulatory T cells during vaccination with hTERT (class I and II) peptide-pulsed DCs" pot
Ngày tải lên :
18/06/2014, 15:20
... DCTs and DCTIs have an optimal antigen uptake capacity and upregulation of CD86, CD40 and class I, but low levels of CD83, as is expected of non-terminally mature DCs The phenotypic profile of ... MA, Lyerly HK, Gilboa E, Thomas E, Nair SK: Optimization of the sequence of antigen loading and CD40 -ligand-induced maturation of dendritic cells Cancer Res 1998, 58:2965-2968 Schaft N, Dorrie J, ... JME, ME: conception and logistics of the study MMA, CV, SS, RAR, SC AJM: vaccination of patients, acquisition of samples and generation of data MMA, AJM, NRH, JB, FF: preparation of the vaccine MMA,...
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Báo cáo y học: "A paragon of self-tolerance: CD25+CD4+ regulatory T cells and the control of immune responses" pps
Ngày tải lên :
09/08/2014, 01:23
... naturally occurring CD25 +CD4+ population could account for the regulatory effect of CD5high and CD45 RBlow CD4+ T cells A comprehensive characterisation of the surface profile of TR cells has revealed ... genetic defects of Foxp3 and the experimental depletion of CD25 +CD4+ TR cells led several groups to investigate the potential role of Foxp3 in the development and function of TR cells Three independent ... elevated levels of activa- 21 Arthritis Research & Therapy Vol No Fehérvári and Sakaguchi Table Comparison of the phenotype of conventional naïve CD4+ T cells and CD4+ regulatory cells (TR) Conventional...
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Báo cáo y học: "Regulating the immune system: the induction of regulatory T cells in the periphery Jane H Buckner1 and Steven F Ziegler2" pot
Ngày tải lên :
09/08/2014, 01:24
... allogeneic dendritic cells [20] Induction of TR cells from CD4+ CD25– T cells has also been successful by activation of CD4+ CD25– T cells with mature, allogeneic dendritic cells, and these T cells ... TR cells lead to the induction of T regulator (Tr1) cells and Th3 cells, which feed back to inhibit inflammation, and the TR cells inhibit proliferation of antigen-specific and bystander T cells ... to antigen and costimulation These cells proliferate and become CD4 T effectors Activation also induces a subset of CD4+ CD25– T cells to upregulate CD25 and FoxP3 and acquire CD25 +CD4+ (TR) cell...
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Báo cáo y học: "The potential of human regulatory T cells generated ex vivo as a treatment for lupus and other chronic inflammatory diseases" pot
Ngày tải lên :
09/08/2014, 03:24
... IL-2-activated T cells These effects include upregulation of CD25, CTLA-4 and CD40 ligand expression on CD4+ cells [14,47], and increased tumor necrosis factor-α production by both CD4+ and CD8+ cells [47] ... naive fraction of CD4+ cells to expand and to become contact-dependent ‘professional’ regulatory T cells TGF-β also induces CD4+ and CD8+ cells that are CD25– to become Th3-like cells Although ... have been called ‘professional’ suppressor cells [5,8] In general, the properties of rodent and human CD4+ CD25+ T cells appear to be very similar In humans, 6–18% of CD4+ T cells constitutively...
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Báo cáo y học: "The role of regulatory T cells in antigen-induced arthritis: aggravation of arthritis after depletion and amelioration after transfer of CD4+CD25+ T cells" doc
Ngày tải lên :
09/08/2014, 06:22
... the migration behaviour of CD4+ CD25+ and CD4+ CD25- cells in vivo For these experiments CD4+ cells were enriched by negative selection and sorted by FACS into CD4+ CD25+ and CD4+ CD25- populations ... cells had higher levels of IgGs (Fig 6d) R296 Figure Modulation of antigen-induced arthritis (AIA) by transfer of regulatory T cells (Treg cells) cells (Treg cells) Amelioration of clinical and ... Migration behaviour of regulatory T cells (Treg cells) (a) CD4+ CD25+ cells) and CD4+ CD25- cells were purified by fluorescence-activated cell sorting (FACS) and labelled with 111In Cells (1 × 106)...
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Báo cáo y học: "β Transforming growth factor-β-induced regulatory T cells referee inflammatory and autoimmune diseases" potx
Ngày tải lên :
09/08/2014, 06:22
... vitro, and immunosuppressive The absence of Treg results in the breakdown of tolerance and the development of autoimmune diseases [28] Our understanding of the functional domain of these cells ... secretion by dendritic cells and macrophages, and TGF-βtreated APCs facilitate the generation of regulatory T cells [38], creating an environment favorable for the induction of suppression and/ or tolerance ... that Treg can be expanded and/ or induced de novo from CD4+ CD25– precursor T cells How, where, and if the size and function of this population can be intentionally controlled is of the utmost importance...
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Báo cáo y học: "Role of regulatory T cells in experimental arthritis and implications for clinical use" potx
Ngày tải lên :
09/08/2014, 06:23
... dampening of joint inflammation and the reinstatement of fully functional CD4+ CD25+ Treg, RA is still not cured These strands of evidence seem to play down a hypothetical therapeutic role of CD4+ CD25+ ... the area of CD4+ CD25+ Treg? One possibility is to investigate the use of antigen-specific CD4+ CD25+ Treg Indeed, it has been reported that antigen-specific CD4+ CD25 Treg, generated and expanded ... production of TNF-α and interferon-γ [18] However, the presence of functionally efficient peripheral CD4+ CD25+ Treg after antiTNF-α therapy might be due to a simple redistribution of these cells, ...
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Báo cáo y học: "Regulatory T cells in systemic lupus erythematosus: past, present and future" pot
Ngày tải lên :
09/08/2014, 13:22
... attention from CD8+ Tregs to CD4+ Tregs, published studies of CD4+ CD25+ cells and CD4+ Foxp3+ cells in human SLE have been apparently contradictory Early reports of CD4+ CD25+ T cells in human SLE revealed ... of accessory cells in the assays (see above) Studies of CD4+ Foxp3+ cells in SLE have been even more variable that those on CD4+ CD25+ cells Two groups have reported decreased CD4+ CD25+Foxp3+ cells ... plasmacytoid dendritic cells that cause expansion of autoantigen-specific regulatory T cells and contraction of inflammatory Th17 cells J Immunol 2007, 178: 7849-7858 Wu HY, Staines NA: A deficiency of CD4+ CD25+...
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Báo cáo y học: "Japanese Encephalitis Virus wild strain infection suppresses dendritic cells maturation and function, and causes the expansion of regulatory T cells" doc
Ngày tải lên :
11/08/2014, 21:21
... penicillin, 10 ng/ml of rmGMCSF and rmIL-4) At day and of culturing, 50% of the supernatant was removed and replenished with fresh DCs media At day 6, non-adherent cells were collected and transferred ... Dittmer U: Friend retrovirus infection of myeloid dendritic cells impairs maturation, prolongs contact to naı¨ve T cells, and favors expansion of regulatory T cells Blood 2007, 110:3949-3958 Moutaftsi ... Differentiation of Tr1 cells by immature dendritic cells requires IL-10 but not CD25 +CD4+ Tr cells Blood 2005, 105:1162-1169 Fontenot JD, Gavin MA, Rudensky AY: Foxp3 programs the development and function of...
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Báo cáo y học: " CD39+ Regulatory T cells suppress generation and differentiation of Th17 cells in human malignant pleural effusion via a LAP-dependent mechanism" pps
Ngày tải lên :
12/08/2014, 13:22
... of naïve CD4+ T cells was > 97%, as measured by flow cytometry CD4 + T cells were also stained with CD4- PerCPCy5.5, CD25-PE, and CD39-FITC, (eBiosciences), and CD4+ CD25highCD39+ T cells and CD4+ CD25- ... generation and differentiation of Tregs or /and Th17 cells in MPE To evaluate the contribution of cytokines to the numbers of pleural CD39+Tregs and Th17 cells, we purified naïve CD4+ T cells from MPE and ... CD4+ T cells Comparisons of percentages of CD39+Tregs (D), Th17 cells (E), and ratios of CD39+Tregs/Th17 cells (F) in MPE and blood from patients with lung cancer (n = 16) The percentages of CD39+Tregs...
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Báo cáo y học: "Isolated receptor binding domains of HTLV-1 and HTLV-2 envelopes bind Glut-1 on activated CD4+ and CD8+ T cells" pot
Ngày tải lên :
13/08/2014, 09:21
... Glut-1 expression and concomitant glucose uptake in CD4 and CD8 lymphocytes: Induction of H1RBD and H2RBD binding CD4+ and CD8+ T lymphocytes were isolated by negative selection and stimulated via ... by the ability of cells to uptake nonhydrolyzable 2-deoxy-D [1-3H]glucose, was at the limits of detection in both quiescent CD4 and CD8 T cells and increased by >20-fold in both subsets following ... Glucose uptake in quiescent CD4 T cells, CD3/ CD28-activated CD4 T cells and cytochalasin B-treated CD3/CD28-activated CD4 T cells was assessed as described in the legend of figure Uptake for each...
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Báo cáo y học: "Signatures of human regulatory T cells: an encounter with old friends and new" pdf
Ngày tải lên :
14/08/2014, 16:21
... profiling of CD4+ CD25+ TReg and CD4+ CD25- naïve T cells Figure Transcriptional profiling of CD4+ CD25+ TReg and CD4+ CD25- naïve T cells To identify molecular differences between regulatory and ... phenotype and the amount of Foxp3+ cells within MACS purified human CD4+ CD25+ T cells (Additional data file 5); and a Word table describing the phenotype of contaminating cells within MACS purified CD4+ CD25+ ... labeled with anti -CD4 and anti-CD25 Labeled cells were separated with a MoFlo and purity was in excess of 98% Isolated cells were pooled equivalently (three independent individuals) and subsequently...
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Báo cáo sinh học: " Protection against the allergic airway inflammation depends on the modulation of spleen dendritic cell function and induction of regulatory T cells in mice" docx
Ngày tải lên :
14/08/2014, 19:22
... OVA-sensitization and challenge could induced the development of airway inflammation and Th2 responses and suppressed the proliferation of peripheral CD4+ and CD8+ T cells and the expression of Foxp3+ ... http://www.gvt-journal.com/content/8/1/2 Figure Proliferation of peripheral CD4+ (A) and CD8+ T cells (B) and the expression of Foxp3+ on spleen CD4+ T cells (C) harvested from PBS-vaccinated mice sensitized and challenged with PBS, ... detaching, CD4+ T cells were stained with CD25 MicroBeads (Miltenyi Biotec) and CD4+ CD25+T cells were positively and negatively selected Separation was controlled by FCM and Spleen CD4+ T cells were...
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Tài liệu Lab 4.2.3 Suspending and Disconnecting Telnet Sessions docx
Ngày tải lên :
11/12/2013, 14:15
... suspended Telnet session, type disconnect and press Enter Upon completion of the previous steps, logoff by typing exit Turn the router off 2-4 CCNA 2: Routers and Routing Basics v 3.0 - Lab 4.2.3 ... interfaces This will identify the type of router as well as how many interfaces the router has There is no way to effectively list all of the combinations of configurations for each router class ... possible combinations of interfaces in the device This interface chart does not include any other type of interface even though a specific router may contain one An example of this might be an...
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