cd34 hematopoietic progenitor cells and high efficiency vector transduction

Báo cáo hóa học: " Nutraceutical augmentation of circulating endothelial progenitor cells and hematopoietic stem cells in human subjects" potx

... growth factor stimulated and not stimulated cells was calculated and compared for different periods before and after intervention Stem-Kine mobilizes CD34 and CD133 Cells Statistics Differences ... approximately 53% in the number of CD34 expressing cells and and a 90% increase in CD133 cells in circulation Furthermore, a significant augmentation of cells possessing hematopoietic colony forming ... Additional file 1: Progenitor Cell Numbers Expressed as a Percentage of Peripheral Blood Mononuclear Cells The data provided represent number of progenitor cells (CD133, CD34, and cells with EPC...

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Báo cáo y học: " Lentiviral transduction of Tar Decoy and CCR5 ribozyme into CD34+ progenitor cells and derivation of HIV-1 resistant T cells and macrophages" pdf

Danh mục: Báo cáo khoa học

... progenitor cells and high efficiency vector transduction Human fetal liver CD34+ hematopoietic progenitor cells (HPC) were purified by positive selection on a magnetic column using the Direct CD34 Progenitor ... CD34+ purity and transduction efficiency CD34+ cell purity and transduction efficiency: CD34+ cells derived from human fetal liver were purified by immunomagnetic beads and assayed by FACS Percentage ... in CD34+ hematopoietic progenitor cells by using lentiviral vectors The transduced progenitor cells differentiated normally into mature thymocytes in vivo in thy/liv grafts of SCID-hu mice and...

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Augmentation and differentiation of hematopoietic progenitor cells by CD137

Danh mục: Tổng hợp

... 1.1 Hematopoietic stem and progenitor cells (HSPC) Stem cells hold great promise for regenerative medicine and the study of cell and tissue differentiation Hematopoietic stem and progenitor cells ... marrow cells and Lin-, CD117+ cells 3.2.3 CD137 ligand signaling induces colony formation in 87 hematopoietic progenitor cells 3.2.4 Expression of CD137 and CD137 ligand on bone marrow cells 91 ... subsets of bone marrow cells Activation of hematopoietic progenitor cells – CD34+ cells in the human system and Lin-, CD117+ cells in the murine system – through CD137 ligand induces activation,...

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Báo cáo sinh học: " CNS progenitor cells and oligodendrocytes are targets of chemotherapeutic agents in vitro and in vivo" pdf

Danh mục: Báo cáo khoa học

... cells in the SVZ and DG are doublecortin+ (DCX+) neuronal progenitors [44], and that other TUNEL+ cells include GFAP + cells (which may be stem cells or astrocytes [45]) and NG2+ progenitor cells ... cells caused 70-100% reduction in viability of O-2A/OPCs, GRP cells, NRP cells, and NSCs (Figure 3) The preferential vulnerability of both rat and human primary CNS progenitor cells to BCNU and ... neural cells (rat) 100 120 Primary neural cells (human) 20 40 60 80 Percent survival 100 120 Cancer cells Figure Primary CNS cells are more vulnerable to BCNU and cisplatin than are cancer cells Cells...

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Báo cáo y học: "Hematopoietic stem cells and retroviral infection" potx

Danh mục: Báo cáo khoa học

... expression of CD34 and represents a heterogeneous cell population encompassing stem cells, early pluripotent progenitor cells, multipotent progenitor cells, and uncommitted differentiating cells [8] ... blood CD34( +) cells and modulates cell cycle and proliferation of mouse hematopoietic progenitor cells Blood 2002, 100:2463-2471 Fukuda S, Mantel CR, Pelus LM: Survivin regulates hematopoietic progenitor ... CD14+ monocytes and CD33+ myeloid precursor cells [59,60] However HCMV can also infect CD34+ hematopoietic progenitor populations, and viral DNA sequences can be detected in CD34+ cells from healthy...

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Báo cáo y học: " Effect of erythropoietin on level of circulating endothelial progenitor cells and outcome in patients after acute ischemic stroke" ppsx

Danh mục: Báo cáo khoa học

... (FITC)-conjugated CD34 and the phycoerythrin (PE)-conjugated CD31, and CD62E (Becton Dickinson, San Jose, CA, USA) to determine the EPC surface markers of CD31 /CD34 (E1), CD62E /CD34 (E2), and KDR /CD34 (E3), ... contributions HKY and THT designed the experiment, drafted and performed laboratory work CKS and SL were responsible for the laboratory assay and troubleshooting HSL, SFC, CMY, TYT and MYL supervised ... aspects and participated in patient recruitment CWL and CHL participated in neurological function assessment WNC and HKY participated in refinement of experiment protocol and coordination and helped...

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Vascularised bone tissue engineering endothelial progenitor cells and human mesenchymal stem cells coculture in 3d honeycomb scaffolds and the effect of bi rotational bioreactor and hypoxic microenvironment 1

Danh mục: Y - Dược

... cells and its progenitor cells Table 2-10 Comparison between UCB and adult PB-EPC in terms of its colony emergence, growth and vessel forming ability Table 2-11 Comparison of the advantages and ... Endothelial Progenitor Cells 52 2.8.2.7 Endothelial Progenitor Cells in Fracture Healing 53 2.8.2.8 Proposed Endothelial Cell Type – Umbilical Cord Blood-Endothelial Progenitor Cells ... flow cytometry showed high expression of MSC markers such as (A-C) CD73, CD90, CD105 and did not express hematopoietic and endothelial markers such as (D-F) CD14, CD34 and CD45 Figure 4-2 Characterisation...

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IMPACT OF ALCAM (CD166) ON HOMING OF HEMATOPOIETIC STEM AND PROGENITOR CELLS Mariya Aleksandrova

Danh mục: Y khoa - Dược

... primitive CD34+ hematopoietic cells (rhodamine 123lo, Thy-1+, CD38-/lo) and myeloid progenitors37, perichondrium (mesenchymal stem cells) 30, endothelial cells2 9, OB38, stromal cells8 , and melanoma cells6 0 ... Mononuclear Cells CFU Colony Forming Units CTV Cell Trace Violet FITC Fluorescein Isothiocyanate HN Hematopoietic Niche HSC Hematopoietic Stem Cells HSPC Hematopoietic Stem and Progenitor Cells Marrow ... receptors distinguish hematopoietic stem and progenitor cells and reveal endothelial niches for stem cells Cell 121, 1109-1121 (2005) 40 CURRICULUM VITAE Mariya Aleksandrova Aleksandrova EDUCATION...

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Characterization of high efficiency pseudo bilayer organic solar cells and

Danh mục: Cao đẳng - Đại học

... processed solar cells and suggested that the high efficiency observed for the solution processed bilayer solar cells is due to the enormous exciton quenching present in the bilayer solar cells (also ... with a higher LUMO energy level of −3.91eV which is 0.17 eV higher than that of PCBM and was used in fabricating solar cells based on P3HT as donor material These solar cells have shown a high ... dye sensitized solar cells, perovskite solar cells and organic solar cells There is a lot of research that is being carried out on them to increase their device efficiencies and life times Figure...

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Analysis and performance of high efficiency synchronous reluctance machines

Danh mục: Môi trường

... of the machine, high reluctance in the quadrature axis and low reluctance in the direct axis It is now well known fact that RSM output increases with high Xd and low Xq (i.e high Xd/Xq) The reluctance ... design is to have a high saliency ratio (Ld/Lq) accompanied by a high (Ld-Lq), i.e a high Ld and low Lq Finite element package is employed to compute the flux distribution and inductances The ... distribution of magnetic and non-magnetic materials can result in high saliency ratio and also high (Ld-Lq), a pre-requisite for improved performance The inductances and the performance of one...

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Báo cáo sinh học: "Safety and feasibility of third-party multipotent adult progenitor cells for immunomodulation therapy after liver transplantation–a phase I study (MISOT-I)" doc

Danh mục: Điện - Điện tử

... adult progenitor cells (MAPCs) with the goal of reducing the dose of immunosuppressive drugs and of supporting liver regeneration in marginal grafts Multipotent adult progenitor cells MAPCs belong ... study is isolated from a single bone marrow aspirate and cultured with heat inactivated fetal bovine serum (FBS) and growth factors EGF and PDGF Cells display a linear expansion rate to 65 population ... transplantation Adherent, non -hematopoietic bone marrow stem cells, including MAPCs and MSCs, have been shown to beneficially modulate the anti-donor immune response in organ transplantation and to promote...

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báo cáo hóa học:" Safety and feasibility of third-party multipotent adult progenitor cells for immunomodulation therapy after liver transplantation–a phase I study (MISOT-I)" pptx

Danh mục: Hóa học - Dầu khí

... adult progenitor cells (MAPCs) with the goal of reducing the dose of immunosuppressive drugs and of supporting liver regeneration in marginal grafts Multipotent adult progenitor cells MAPCs belong ... study is isolated from a single bone marrow aspirate and cultured with heat inactivated fetal bovine serum (FBS) and growth factors EGF and PDGF Cells display a linear expansion rate to 65 population ... transplantation Adherent, non -hematopoietic bone marrow stem cells, including MAPCs and MSCs, have been shown to beneficially modulate the anti-donor immune response in organ transplantation and to promote...

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Báo cáo hóa học: "Two novel low-power and high-speed dynamic carbon nanotube full-adder cells" pdf

Danh mục: Hóa học - Dầu khí

... this figure, to obtain more efficiency and enhancing the proposed design, we eliminate NAND gate and replace the NMOS T4 transistor with a PMOS transistor Vdd (TB) with high threshold, Vt = 2.5v, ... “0” and COUT output is unchanged and it is equal to “1.” At this phase when SIGMA is “1,” the T1, T2, and T3 transistors are “off.” As a result, both outputs, SUM and COUT, are unchanged and ... Moaiyeri MH, Navi K: High speed NP-CMOS and multi-output dynamic full adder cells International Journal of Electrical, Computer, and Systems Engineering 2010, 4:4 Bagherizadeh and Eshghi Nanoscale...

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Báo cáo y học: "Reduced number and impaired function of circulating progenitor cells in patients with systemic lupus erythematosus" pot

Danh mục: Báo cáo khoa học

... plates and incubated at 37°C, 5% CO2 overnight Cells were gently washed with PBS and the number of attached cells was determined in ten high power fields per well The average number of cells per high ... single-positive for CD14, CD34 and CD133 and co-stained for CD34 and CD133 were quantified by fluorescence activated cell sorting and are depicted here as the amount of positively stained cells per milliliter ... expressed on hematopoietic progenitor cells, but not on mature endothelial cells [19,21] During maturation of these cells, the expression of CD133 is lost The combined expression of CD34 and CD133,...

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Báo cáo y học: " Enhanced self-renewal of hematopoietic stem/progenitor cells mediated by the stem cell gene Sall4" doc

Danh mục: Báo cáo khoa học

... transplanted with Sall4 expanded cells Strain Number of mouse C57B/6 Sall4A expanded cells, × 106 17 WT-like B6.SJL Sall4A expanded cells, × 106 15 WT-like B6.SJL Sall4A expanded cells, × 106 11 WT-like ... differentiated cells to ESC-like cells, and generation of induced pluripotent stem cells (iPS) [11-13] We and others have determined that Sall4 exists as two isoforms (Sall4A and Sall4B), and they ... Sall4B expanded cells, × 10 15 WT-like B6.SJL Sall4B expanded cells, × 106 11 WT-like B6.SJL GFP-infected cells, × 106 15 WT-like 11 WT-like B6.SJL Number of injected cells GFP-infected cells, ×...

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Báo cáo y học: "Sequestration and homing of bone marrow-derived lineage negative progenitor cells in the lung during pneumococcal pneumonia" ppsx

Danh mục: Báo cáo khoa học

... several (such as G-CSF and GM-CSF) are known to release hematopoietic stem cells from the BM [11,12] Recent studies have shown that these BM-derived stem cells or progenitor cells have the ability ... in donor cells A representative image and an emission scan of QDlabeled donor cells are shown in Figure 1A and 1B Fluorescent particles were detected within the cytoplasm of donor cells and the ... tissues and rarely migrated into the alveolar air spaces, suggesting that BM-derived progenitor cells sequester and home to lung tissues Several studies have shown increased circulating progenitor cells...

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Báo cáo y học: "Relative percentage and zonal distribution of mesenchymal progenitor cells in human osteoarthritic and normal cartilage" potx

Danh mục: Báo cáo khoa học

... morphology and migration and participates in developmental processes It has been found on a variety of cells such as endothelial cells, activated macrophages, fibroblasts, smooth muscle cells, and the ... superfamily and a ligand for CD6, which is involved in T-cell adhesion and co-stimulation [17] Besides being expressed on thymic epithelial cells, activated T cells, Blymphocytes, and monocytes, ... and colleagues [21] (see Materials and methods) Interestingly, the distribution of the CD166+ cells in OA and normal samples was not uniform but was clearly zonal (Figures and 7) In both OA and...

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Báo cáo y học: " Reconstitution of the myeloid and lymphoid compartments after the transplantation of autologous and genetically modified CD34+ bone marrow cells, following gamma irradiation in cynomolgus macaques" potx

Danh mục: Báo cáo khoa học

... Reconstitution with transduced autologous CD34+ cells in irradiated cynomolgus macaques Monkeys CD34+ cells purity CD34+ cells collected CD34+ cells transduced CD34+ cells infused/kg 6653 6833 6896 7036 ... each animal B: Transduction of bone marrow progenitor cells with an SIV-based vector CD34+ cells were cultured in the presence of cytokines (see materials and methods) and exposed to vector particles ... Efficient transduction of cynomolgus macaque CD34+ bone marrow cells We first assessed, in vitro, the efficiency with which a SIVmac251-derived vector transduced CD34+ hematopoietic cells from...

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Báo cáo y học: "The change and effect of endothelial progenitor cells in pig with multiple organ dysfunction syndromes" ppt

Danh mục: Báo cáo khoa học

... as cells positive for both hematopoietic stem cell markers and endothelial markers, such as CD133, CD34 and KDR CD133 and CD34 were deemed the two main hematopoietic stem cell markers Unlike CD34, ... endothelial progenitor cells in syndromes (left) peripheral blood and (right) bone marrow *P < 0.05, **P < 0.01 more than 90% of the cultured cells were positive for KDR and CD133 (Figure 3) Migratory and ... endothelial progenitor cells The attached spindle cells at different points were all positive for taking up Dil-ac-LDL and UEA-1 Co-staining cells revealed that more than 90% of adherent cells are...

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Báo cáo sinh học: " Improved gene delivery to human saphenous vein cells and tissue using a peptide-modified adenoviral vector Lorraine M Work1, Paul N Reynolds2 and Andrew H " pptx

Danh mục: Báo cáo khoa học

... http://www.gvt-journal.com/content/2/1/14 Figure Transduction of saphenous vein cells and intact tissue Transduction of saphenous vein cells and intact tissue Ad-CTL and Ad-RGD expressing eGFP were incubated with (A) HSVEC and (B) HSVSMC ... Ad vectors for 10, 30, 60 mins at 37°C The cells were washed twice in PBS and the media changed 24 hours post-infection, the cells were again washed in PBS, lysed in PBS/0.2% Triton-X-100 and transduction ... endothelial and smooth muscle cells during graft Page of (page number not for citation purposes) Genetic Vaccines and Therapy 2004, 2:14 gene delivery [9], and here show increased transduction...

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