cd34 cell into immunodeficient mice
báo cáo khoa học: "Hepatocyte growth factor incorporated chitosan nanoparticles augment the differentiation of stem cell into hepatocytes for the recovery of liver cirrhosis in mice" pptx
... derivedstem cells Cell 2001, 105:369-377 Afonso FA, Siapati EK, Bonnet D: In vivo contribution of murine mesenchymal stem cells into multiple cell- types under minimal damageconditions J Cell Sci ... understood Cell fusion, in which stem cells fuse with the somatic cell in the niche, had been suggested by many authors [11] and strong evidences were reported for the transdifferentiation of stem cells ... recovery after the transplantation of stem cells are augmented by the delivery of bioactive molecules that induce stem cells to differentiate into specific-lineage cells HGF has been reported to be a...
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Báo cáo y học: "CXCR4 and CCR5 shRNA transgenic CD34+ cell derived macrophages are functionally normal and resist HIV-1 infection" pdf
... targeted to these coreceptors into primary CD34+ hematopoietic progenitor cells which can give rise to all the blood cell lineages including macrophages, T cells, and dendritic cells Since siRNAs are ... Auburn, CA)[8] The purity of CD34+ cells was determined by FACS using a PE conjugated CD34+ antibody The purity of cells was routinely >93% (data not shown) CD34+ cells were maintained in Iscove's ... gene transduced CD34+ cells into macrophages The transgenic macrophages showed significant down regulation of the respective targeted coreceptors CXCR4 and CCR5 Thus, differentiated cells retained...
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the role of fgfr3 mutation in tumour initiation, progression and invasion of urothelial cell carcinoma in mice
... suggesting the presence of urothelial stem cells Stem cells are unspecialised cells that have the ability to self-renew and to differentiate into several cell types (Weiner, 2008) In other epithelia ... factor EGFR Epidermal growth factor receptor ERK Extracellular signal regulated kinase ES cells Embryonic stem cells FACS Fluorescence-activated cell sorting FANFT N-(4,5-nitro-2-furyl-2-thiazolyl)-formamide ... human urothelium consists of four to seven cell layers, including a single basal cell layer, multiple intermediate cell layers, as well as a single umbrella cell layer facing the lumen The murine...
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Numerical investigation into premixed hydrogen combustion within two-stage porous media burner of 1 kW solid oxide fuel cell system
... Foulker F R., Fuel Cell Handbook, Van Norstand Reinhold, New York, 1989 [2] Stanley W A., Direct Energy Conversion, Allyn and Bacon, Inc., Boston, 1982 [3] Larminie J., Dicks A., Fuel Cell Systems ... solid oxide fuel cell power generating system since 2005 His main research interests include critical heat flux, boiling heat transfer, cold neutron source, and solid oxide fuel cell E-mail address:chlee@iner.gov.tw ... numerical simulations provide a more convenient and versatile means of obtaining detailed insights into the characteristic thermofluidic properties of the stack and afterburner components of an SOFC...
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Tài liệu Báo cáo khoa học: Differentiation stage-dependent preferred uptake of basolateral (systemic) glutamine into Caco-2 cells results in its accumulation in proteins with a role in cell–cell interaction pptx
... flexible intercellular adhesive contacts outside the junctional complexes [49] In addition, galectin-3 is suggested to be involved in cell cell and cell matrix interactions It is an intracellular ... and extracellular lectin, which interacts with intracellular glycoproteins, cell surface proteins and extracellular matrix proteins Overexpression of galectin-3 in human breast carcinoma cell lines ... examining glutamine incorporation into cellular proteins The Caco-2 cell line was used for this study Although originally derived from a human colon adenocarcinoma, the cells undergo spontaneous enterocytic...
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Understanding the participatory news consumer - How internet and cell phone users have turned news into a social experience pdf
... their cell 68% get news and current events information on their cell 49% have downloaded an application that allows them to access news, weather, sports, or other information on their cell 44% ... unmistakable One quarter (26%) of all Americans say they get some form of news via cell phone today–that amounts to 33% of cell phone owners These wireless news consumers get the following types of news ... organizations, or individuals About the Pew Research Center’s Project for Excellence in Journalism The Pew Research Center's Project for Excellence in Journalism is dedicated to trying to understand the...
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Báo cáo khoa học: Intrabodies against the EVH1 domain of Wiskott–Aldrich syndrome protein inhibit T cell receptor signaling in transgenic mice T cells potx
... Fig Subcellular localization of anti-WASP scFvs Cell extracts of (A) anti-WASP scFvs transgenic T cells and (B) anti-WASP scFv DNA-transfected NIH-3T3 cells were fractionated into the subcellular ... signaling Subcellular localization of anti-WASP scFvs To examine the subcellular localization of anti-WASP scFvs 21SHL and 21SHL-CL in T cells, cell extracts of their scFv-transgenic T cells were ... stimulated T cells was determined by counting the number of caps in 200 cells ⁄ experiment Subcellular localization of scFv intrabodies Cell extracts of anti-WASP scFv transgenic T cells and scFv...
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Báo cáo khoa học: Injection of poly(b-L-malate) into the plasmodium of Physarum polycephalum shortens the cell cycle and increases the growth rate pot
... the cytoplasm during S-phase and G2-phase of the cell cycle but not in G1-phase have been reported for HeLa cells [26] In some other mammalian cells, examples of cytoplasmic accumulation of H1, ... histone H1 during HeLa cell cycle Chromosoma 108, 308–816 27 Zlatanova, J.S., Srebreva, L.N., Banchev, T.B & Tsanev, K.G (1990) Cytoplasmic pool of histone H1 in mammalian cells J Cell Sci 96, 461–465 ... Shochat (1980) [22] Results and Discussion Growth rate and duration of cell cycle In the intial experiments, PMLA was injected into macroplasmodia, and growth was measured in comparison to control...
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Báo cáo khoa học: The molecular chaperone a-crystallin incorporated into red cell ghosts protects membrane Na/K-ATPase against glycation and oxidative stress ppt
... ghost cells with equal efficiency (result not shown) To confirm that the lysing and resealing procedure produced effectively sealed ghost cells, 14C sucrose was resealed into ghost cells The cells ... ghost cells eluted in the void volume (70 mL) while the main haemoglobin band followed about 130 mL later (Fig 1) The lysed cells were white and therefore practically haemoglobin-free The lysed cells ... after h Resealed ghost cells, with and without incorporated a-crystallin, were suspended in Mops buffer to give a final haematocrit of 10% Resealed cells were aliquotted into 1.5-mL Eppendorf...
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Báo cáo khoa học: Introduction of extended LEC14-type branching into core-fucosylated biantennary N-glycan Glycoengineering for enhanced cell binding and serum clearance of the neoglycoprotein pot
... this level of testing The decrease of cell positivity for KG-1 cells and rather constant results for K-562 cells can be judged as internal controls for cell- type specificity in the direct comparison ... lectin ligand cell surface binding was performed with the following human tumor lines: Croco II (B-lymphoblastoid cell line), CCRF-CEM (T-lymphoblastoid cell line), K-562 (erythroleukemia cell line), ... leukemia cell line), DU4475 (mammary carcinoma cell line) as well as C205, SW480 and SW620 (colon adenocarcinoma cell lines) Except for the Croco II line established in our laboratory [56] the cells...
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Báo cáo khoa học: Assembly of collagen types II, IX and XI into nascent hetero-fibrils by a rat chondrocyte cell line ppt
... Transmission electron microscopy of the cell layer identified cells that retained the organelles and morphology typical of chondrocytes (Fig 1) In Fig 1A, the RCSLTC cells show oval-shaped mitochondria, ... understand better the temporal sequence and mechanism of assembly Materials and methods Cell culture The RCS-LTC cell line was maintained in monolayer culture in high-glucose DMEM (Dulbecco’s modified ... electron-microscopy of cultured chondrosarcoma cells and surrounding matrix (A) RCS-LTC chondrocyte Note the presence of thin filamentous fibrils pericellularly (arrowhead) Bar ¼ lm (B) Parent Swarm...
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báo cáo hóa học:" Regression of orthotopic neuroblastoma in mice by targeting the endothelial and tumor cell compartments" pdf
... of nM CHS 828 for to 72 h Cell morphology, investigated by phasecontrast microscopy, revealed signs of cell death in NB cells exposed to CHS 828 (A) Viability of NB cells (IMR-32) was quantified ... NB cells To address the question whether CHS 828 was less active in cell lines with greater avidity for MIBG, we included the NB cell line SK-N-SH in our in vitro toxicity studies CHS caused cell ... considered low for the host cells (including the endothelial cells) but higher for the human tumor cells Despite this, both tumor vessels of murine origin and human tumor cells were affected by treatment...
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Báo cáo sinh học: "Curcumin reduces expression of Bcl-2, leading to apoptosis in daunorubicin-insensitive CD34+ acute myeloid leukemia cell lines and primary sorted CD34+ acute myeloid leukemia cells" pptx
... of CD34+ cells The percentages of CD34+ cells were 99.43 ± 0.60% in KG1a cells, 96.67 ± 0.11% in Kasumi-1 cells, but CD34+ was absent in U937 cells (Figure 1A) After treatment of these three cell ... these immature phenotype CD34+ AML progenitors to current chemotherapies CD34 + AML cells are 10-15-fold more resistant to DNR than CD34- AML cells [8] CD34+ KG1a and TF-1 AML cell lines are 30-40 ... leukemia CD34+ cells [14] CD34+ AML cells have higher levels of Bcl-2 gene and protein than CD34- AML cells [6] DNR-induced apoptosis can be blocked by Bcl-2 overexpression in DNRsensitive CD34- ...
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Báo cáo sinh học: " Spectratyping analysis of the islet-reactive T cell repertoire in diabetic NOD Igμnull mice after polyclonal B cell reconstitution" potx
... the islets of NOD.Igμ null mice that are dependent on B cells We observed that without B cell reconstitution, NOD.Igμnull mice had no pancreatic T cell expansion No T cell receptor PCR product ... Importantly, NOD Igμ null mice have been shown to contain a functional autoimmune T cell repertoire (in the absence of B cells) capable of causing diabetes if transferred into NOD.scid mice [8] CDR3 spectratyping ... NOD.Igμnull mice reconstituted with NOD splenic B cells We studied the progression of diabetes in > 100 NOD Igμnull mice reconstituted with NOD splenic B cells in comparison to controls (mice receiving...
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o cáo hóa học:" Spectratyping analysis of the islet-reactive T cell repertoire in diabetic NOD Igμnull mice after polyclonal B cell reconstitution" docx
... the islets of NOD.Igμ null mice that are dependent on B cells We observed that without B cell reconstitution, NOD.Igμnull mice had no pancreatic T cell expansion No T cell receptor PCR product ... Importantly, NOD Igμ null mice have been shown to contain a functional autoimmune T cell repertoire (in the absence of B cells) capable of causing diabetes if transferred into NOD.scid mice [8] CDR3 spectratyping ... NOD.Igμnull mice reconstituted with NOD splenic B cells We studied the progression of diabetes in > 100 NOD Igμnull mice reconstituted with NOD splenic B cells in comparison to controls (mice receiving...
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Báo cáo hóa học: "Self-assembly of micelles into designed networks" docx
... 1-I The silver-silver interaction ensembles micelles into aggregates, which further reorganize into vesicles The vesicular structure ensembled from micelles was illustrated in Scheme 2, due to ... binary mixture [3] which arises the re-organization of micelles into vesicles induced by silver nanoparticles From the fluid state, in which micelles move randomly and ceaselessly, to the ordered ... corona-surrounded domain of the templated micelle was incorporated by silver, due to the hydrophilic interactions between silver and ethylene oxide The diameter of the micelle varies over the range of 12–20...
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Using JavaTM ME Platform to Put the Fun into Your Mobile Device and Cell Phone pdf
... ME Platform to Put the Fun into Your Mobile Device and Cell Phone Carol Hamer Creating Mobile Games: Using Java™ ME Platform to Put the Fun into Your Mobile Device and Cell Phone Copyright © 2007 ... can pull out my cell phone and demonstrate some of the games I’ve written for it It’s a great time to be a software developer because a pastime that’s so much fun—writing games for cell phones and ... growth industry The number of cell phones worldwide is well over billion, dwarfing the market of other high-tech items such as personal computers The number of Java-enabled cell phones is in the hundreds...
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Báo cáo khoa học: "Mucosal mast cell-derived chondroitin sulphate levels in and worm expulsion from FcRγ-knockout mice following oral challenge with Strongyloides venezuelensis" doc
... 1987, 9, 31-38 Abe T, Nawa Y Localisation of mucosal mast cells in W/Wv mice after reconstitution with bone marrow cells or cultured mast cells, and its relation to the protective capacity to Strongyloides ... and WT mice were primed as stated above For challenge, primed KO and WT mice with 10 each of their naïve controls were infected as described above The challenge groups were sacrificed mice each ... than KO mice on these days (p < 0.05) In contrast, significantly higher concentrations of these Table (a) Mucosal mast cell number/10 villus crypt unit (Mean ± SD) in FcRγ-KO and WT mice challenged...
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Báo cáo y học: "Insights into spatial configuration of a galactosylated epitope required to trigger arthritogenic T-cell receptors specific for the sugar moiety" ppsx
... T hybridomas Antigen-presenting cells (APCs) used were either DBA/1 irradiated spleen cells (5 × 105 cells/well) or paraformaldehyde-fixed M12.C10 cells (105 cells/well), and an I-Aq+ B lymphoma ... CD4+ T -cell hybridomas used were derived from different mice and thus represent individual clones The anti-CII T -cell clone A9.2 was isolated from the lymph nodes of CII-immunized DBA/1 mice [15] ... cells and hybridomas Three anti-CII CD4+ T -cell hybridomas (A2G10, A8E2 and A9E5) were used in the present study They were derived by fusion of lymph node cells from CII-primed DBA/1 (H-2q) mice...
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