cd34 cell derived macrophages
Báo cáo y học: "CXCR4 and CCR5 shRNA transgenic CD34+ cell derived macrophages are functionally normal and resist HIV-1 infection" pdf
... http://www.retrovirology.com/content/2/1/53 Figure Transgenic macrophages display normal cell surface markers Transgenic macrophages display normal cell surface markers: GFP-alone and XHR transduced CD34+ derived macrophages were labeled ... labeled E.coli by CD34+ derived macrophages Phagocytosis of fluorescently labeled E.coli by CD34+ derived macrophages: E coli Bioparticles® were added directly to the cultured macrophages along ... these coreceptors into primary CD34+ hematopoietic progenitor cells which can give rise to all the blood cell lineages including macrophages, T cells, and dendritic cells Since siRNAs are new tools...
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Tài liệu Báo cáo khoa học: Glycation of low-density lipoprotein results in the time-dependent accumulation of cholesteryl esters and apolipoprotein B-100 protein in primary human monocyte-derived macrophages docx
... cultured mouse macrophage-like cells [29] In the current study, we have determined whether lipid accumulation also occurs in a more relevant cell type ) human monocyte -derived macrophages (HMDMs) ) on ... compared to the control cells (no LDL), LDL plus EDTA-treated cells, and methylglyoxal-modified LDLtreated cells, respectively, at each time point Formation of lipid-laden cells by glycated LDL ... appropriate chase times, cells were lysed and processed to determine nondegraded intracellular apoB (triangles), degraded intracellular apoB (open circles), and degraded extracellular apoB (squares)...
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Báo cáo khoa học: Syndecan-4 is a signaling molecule for stromal cell-derived factor-1 (SDF-1)/ CXCL12 pptx
... not involved in intracellular Ca2+ mobilization induced by SDF1 on HeLa cells Untreated HeLa cells (A), heparitinases I- and III-treated HeLa cells (B), mock-transfected HeLa cells (C), or SD-4 ... der Schoot CE (1999) Increased migration of cord blood -derived CD34+ cells, as compared to bone marrow and mobilized peripheral blood CD34 cells across uncoated or fibronectin-coated filters Exp ... on HeLa cells is HS- and SD-4- dependent To analyze some of the transduction pathways induced by SDF-1 on HeLa cells, whole cell extracts from either unstimulated or stimulated HeLa cells were...
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Báo cáo hóa học: " Modulation of the major histocompatibility complex by neural stem cell-derived neurotrophic factors used for regenerative therapy in a rat model of stroke" ppt
... the class I MHC, mediated by NSC -derived neurotrophins in the lesioned micro-environment of the CNS Materials and methods Culture of Neural stem cells Neural stem cells were harvested from the cortex ... administration or neural stem cell transplant A: reddish brown coloration of caspase III immunoreactivity along the ventricular lining of cells of ischemic rat having undergone neural stem cell transplant ... marrow stem cells in ischemic brain Acta Neurochir Suppl 2006, 99:123-124 Li J, Sun CR, Zhang H, Tsang KS, Li JH, Zhang SD, An YH: Cotransplantation of neural stem cells and Schwann Cells results...
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Báo cáo sinh học: " Role of HIV-1 subtype C envelope V3 to V5 regions in viral entry, coreceptor utilization and replication efficiency in primary T-lymphocytes and monocyte-derived macrophages" docx
... primary blood monocyte -derived macrophages (MDM) Replication of HIV-1 subtype C env V3–V5 region chimeras in primary blood monocyte -derived macrophages (MDM) MDM (0.5 × 106 cells/well) were infected ... patients (Table 1) Replication of subtype C chimeras in primary monocytederived macrophages While primary monocyte derived macrophages (MDM) express CD4 and CCR5 and a low level of CXCR4, they ... region chimeras in T-lymphocyte (A3.01) cell line Replication of HIV-1 subtype C env V3–V5 region chimeras in T-lymphocyte (A3.01) cell line A3.01 cells (1 × 106 cells/well) were infected with equal...
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Báo cáo khoa học: "Mucosal mast cell-derived chondroitin sulphate levels in and worm expulsion from FcRγ-knockout mice following oral challenge with Strongyloides venezuelensis" doc
... References Abe T, Nawa Y Reconstitution of mucosal mast cells in W/ Wv mice by adoptive transfer of bone marrow -derived cultured mast cells and its effects on the protective capacity to Strongyloides ... 1987, 9, 31-38 Abe T, Nawa Y Localisation of mucosal mast cells in W/Wv mice after reconstitution with bone marrow cells or cultured mast cells, and its relation to the protective capacity to Strongyloides ... is not very efficient in establishing patent infection Intestinal mast cell numbers and serum mMCP-1 concentration Mast cells were enumerated in jejunal sections prepared on days and 13 post...
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Báo cáo y học: "Strong inhibition of TNF-α production and inhibition of IL-8 and COX-2 mRNA expression in monocyte-derived macrophages by RWJ 67657, a p38 mitogen-activated protein kinase (MAPK) inhibitor" ppt
... matrix metalloproteinase (MMP9) in monocyte -derived macrophages necrosis factor (TNF)-α, IL-1β, cyte -derived macrophages from healthy controls (n = 5) Cells were stimulated with lipopolysaccharide ... through cell- contact-dependent signalling with several inflammatory cells, mainly T cells and fibroblasts A few soluble stimuli, such as IL-15 and IL-17, are known to have a stimulatory effect on macrophages ... with an IC50 of 0.015 µM for control cells and 0.03 µM for RA cells (Fig 3b) For IL-8 production, the IC50 was 0.3 µM for control cells and 1.2 µM for RA cells IL-6 and MMP9 production was inhibited...
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Báo cáo y học: "Pro-inflammatory properties of stromal cell-derived factor-1 (CXCL12) in collagen-induced arthritis" pptx
... Clark-Lewis) 106 cells were Migrated cells were incubated with anti-CD16/CD32 Fcblocking antibodies (BD Biosciences Pharmingen, San Diego, CA, USA) and washed with PBS After washing, the cells were ... Pharmingen) Cells were washed, fixed with 0.37% formaldehyde in PBS, and analysed by a FACScalibur flow cytometer (Becton Dickinson) Flow cytometric analysis of cells from joint cavities Cells from ... erythrocytes Cells were washed and resuspended in cold PBS Cells were incubated with anti-CD16/ anti-CD32 Fc-receptor-blocking antibodies (BD Biosciences Pharmingen) After washing, the cells were...
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Báo cáo y học: "TNF inhibits production of stromal cell-derived factor 1 by bone stromal cells and increases osteoclast precursor mobilization from bone marrow to peripheral blood" ppt
... Figure Figure cells inhibits stromal cell- derived factor expression by stromal TNF inhibits stromal cell- derived factor expression by ST2ST2 stromal cells ST2 cells, a bone marrow stromal cell line, ... boneinjectiondecreases stromal cell- derived factor expression by TNF injection stromal cellsstromal cell- derived factor expression marrow decreases by bone marrow stromal cells Expression of stromal cell- derived factor ... TNF-transgenic mice stromal cellsstromal cell- derived factor-1 expression in bone marDecreased stromal cell- derived factor-1 expression in bone marrow row stromal cells and bones of TNF-transgenic...
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Báo cáo y học: " Specific antibody response of mice after immunization with COS-7 cell derived avian influenza virus (H5N1) recombinant proteins" ppsx
... target as a candidate avian influenza vaccine The mammalian cell (COS-7 cell line) and prokaryotic cell (E coli) were used as the expression cell system for recombinant HA protein production Also, ... DNA was used for transformation into DH5α competent E Coli cells and pSec-His-HA, pSec-His-NS1 and pSec-His-NA DNA to transform COS-7 cell line by using polyfect transfection system (Qiagen, USA) ... pSec-His-HA, pSec-His-NS1, pSecHis-M and pSec-His-NA in COS-7 cell lines in medium containing 200 µg/ml of hygromycin B were lysed with lysozyme The cell lysate was used to purify recombinant protein with...
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Báo cáo y học: "Interferon-lambda1 induces peripheral blood mononuclear cell-derived chemokines secretion in patients with systemic lupus erythematosus: its correlation with disease activity" potx
... in immune response, influence maturation of kinds of immune cells, such as DC, T cell and stimulate monocytes, NK cells as well as T cell migration together with accommodate adhesion molecule ... IP10/CXCL10 and MIG/CXCL9 production in monocyte -derived dendritic cells and enhances their capacity to attract and stimulate CD8+ effector T cells J Leukoc Biol 2002, 71:669-676 Wada T, Yokoyama ... lymphocytes, and influence maturation of DC, T cell and bone marrow progenitor [20-23] Moreover, they can stimulate monocytes, natural killer (NK) and T cell migration, and modulate adhesion molecule...
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Báo cáo y học: "Mesenchymal stem cell derived hematopoietic cells are permissive to HIV-1 infection" pptx
... exposure on HD cells, we compared the expression of selected genes in HD-HIV cells versus un-infected HD cells The gene profile of HD-HIV cells was then compared to HUT78-HIV cells The analysis ... recrudescence The CD34+ hematopoietic progenitor cells are commonly known to generate macrophages Furthermore, the ability of embryonic stem cells to generate HIV-1 susceptible macrophages has ... exposed HD cells Since cells of the hematopoietic system are among the main targets of the HIV-1 virus, we investigated the effect of viral exposure on HD cells Clonally expanded cells were allowed...
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Báo cáo y học: "Transcriptomic and phenotypic analysis of murine embryonic stem cell derived BMP2+ lineage cells: an insight into mesodermal patterning" ppt
... crest cells and their lineages by BMP2 promoter-driven selection markers paves the way for ES cell- derived neural crest stem cell biology These cells are attractive candidates for future cell ... BMP2-expressing cells during early differentiation of ES cells will pave the way for a better understanding of NCSCs and their differentiation into lineages The BMP2+ cells derived from the ES cells may ... Figure Differentiation of the BMP2+ cells to cardiac cells Differentiation of the BMP2+ cells to cardiac cells (a) RT-PCR analysis of the cardiac markers in BMP2+ cells and other controls (b) Schematic...
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Báo cáo y học: "Global transcriptome analysis of murine embryonic stem cell-derived cardiomyocytes" doc
... analyses (a) Validation of Affymetrix data by quantitative real-time (a) Adhesion Cell cycle Cell death Cell- cell signaling Cellular metabolism reports Development Stress response Signal transduction ... transcripts of interest belonging to the biologic process categories adhesion, cell cycle, cell death, cell- cell signaling, cellular metabolism, development, stress response, signal transduction, transcription, ... (b) The bar chart shows the number of genes in the categories adhesion, cell cycle, cell death, cell- cell signalling, cellular metabolism, development, stress response, signal transduction, transcription,...
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Human pluripotent stem cell derived cellular vehicles for cancer gene therapy
... Stem cells- unique candidates for therapeutic platform 1.2.1 What are stem cells 1.2.1.1 Pluripotent stem cells 19 20 20 1.2.1.1.1 Embryonic stem cells 20 1.2.1.1.2 Induced pluripotent stem cells ... cells in glioma mouse model 70 3.4 Immunomodulation effects of MSC and NSC on tumor growth 76 References 78 Chapter Results: Human Induced Pluripotent Stem Cell- derived Neural Stem Cells as Cellular ... pluripotent stem cell derived neural stem cells Molecular pharmaceutics, Volume 8, Issue (2011) Lee E X , Lam D H , Wu C , Yang J , Tham C K , Ng W H , Wang S 4.Human embryonic stem cell- derived mesenchymal...
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Exploring mesenchymal stem cell derived exosome and tocotrienol (t3) as therapeutic agents in drug induced liver injury (DILI)
... tocotrienol analogs in TAMH cells 104 4.2.1 Cytotoxicity of T3 analogs in TAMH cells 105 4.2.2 Cellular uptake of different concentration of T3 analogs in TAMH cells 107 4.2.3 Summary ... APAP and H2O2-induced on cell death in TAMH cells 112 4.3.2 Effect of lower dosage of -T3 and -T3 against APAP and H2O2-induced injury on cell viability in TAMH cells 118 4.3.3 Effect ... characterized by cell and organelle swelling, extensive cell- content release which follow severe disturbance of cell functions and inflammation Although it is debatable whether hepatic cell death is...
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Use of IPs cell derived neural stem cells as a cellular vehicle for glioma and breast cancer therapy
... pluripotent stem cell derived- neural stem cell human induced pluripotent stem cell derived- neural stem cell expressing CodA human induced pluripotent stem cell derived- neural stem cell expressing Fcy ... stem cell Human embryonic stem cell- derived neural stem cell Herpes simplex virus thymidine kinase Interleukin Intraperitoneal human induced pluripotent stem cell human induced pluripotent stem cell ... cancer 22 1.4 Stem cells 1.4.1 Adult stem cells 1.4.1.1 Neural stem cells 1.4.2 Induced pluripotent stem cells 1.5 Stem cell as a vehicle for cancer gene therapy 1.5.1 Stem cell based cancer gene...
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Human embryonic stem cell derived neural stem cells derivation, differentiation and MicroRNA regulation
... NSC differentiation 1.1.5.2 Pluripotent stem cell- derived NSCs Human embryonic stem cells (hESCs), which are pluripotent cells derived from the inner cell mass of blastocyst-stage embryos, offer ... post-transcriptional repression 29 Figure 3.1 Cells from neurospheres derived from hESC show cellular characteristics of NSCs 56 Figure 3.2 Cells from neurospheres derived from hESC express neuroectodermal ... Glioblastoma multiforme Ganciclovir Glial cell- derived nerve factor Glial fibrillary acidic protein Glial progenitor cell Homeodomain Human embryonic stem cell High mobility group Herpes simplex...
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Role of hodgkin and reed sternberg cell derived lymphotoxin alpha in t cell recruitment into the microenvironment of hodgkin lymphoma lesions
... divide T cells into T helper cells, cytotoxic T cells and regulatory T cells 1.2.1 T helper (TH) Cells There are four basic types of THelper cells: THelper1, THelper2, THelper17 and Treg cells (Figure ... adipocytes, epithelial cells, glial cells, endothelial cells, macrophages and leukocytes The tumor microenvironment is characterized by the crosstalk between tumor cells and different cell types In the ... al., 2013) 1.2 T cells Generally, T cells can be divided into three main classes which are naïve T cells, memory T cells and effector T cells A more detailed classification of T cells based on...
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development of new human stem cell derived cellular vehicles for glioma gene therapy
... Cellular vectors 10 1.3 Stem cell based glioma gene therapy 1.3.1 Stem cells: embryonic and adult 10 11 1.3.1.1 Embryonic stem cells 11 1.3.1.2 Adult stem cells 13 II 1.3.1.3 Embryonic stem cell ... Self-renewing neural stem cells are derived from human 125 125 embryonic stem cells by adherent monoculture 4.3.2 “Stemness” of human embryonic stem cell- derived neural 127 stem cells 4.3.3 In vitro ... glioma tropism evaluation of human embryonic 134 stem cell- derived neural stem cells 4.3.4 Human embryonic stem cell- derived neural stem cells as 137 vectors for glioma gene therapy 4.4 Discussion...
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