cd26 dpp iv expression and dpp iv activity in fibroblasts
Báo cáo y học: "Prostaglandin E2 synthesis in cartilage explants under compression: mPGES-1 is a mechanosensitive gene" docx
Ngày tải lên :
09/08/2014, 08:22
... first intron and is also called COX-1 V1 At this time, its expression is described in both canine and human cortex and aorta, and in the rodent heart, kidney and neuronal tissues [18] Prostaglandin ... demonstrating increased COX-2 gene expression after h and h in compressed explants versus control and no increase for COX-1 and COX-3 Standard curves for COX-1, COX-2, COX-3 and hypoxanthine-guanine ... MD, Goodwin V, Mesnage S, Keelan JA: Cytokineinduced coordinate expression of enzymes of prostaglandin biosynthesis and metabolism: 15-hydroxyprostaglandin dehydrogenase Prostaglandins Leukot...
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Báo cáo sinh học: " Determination of suitable housekeeping genes for normalisation of quantitative real time PCR analysis of cells infected with human immunodeficiency virus and herpes viruses" pdf
Ngày tải lên :
18/06/2014, 18:20
... reflecting variability in both virus strain and cell type Notable differences included 18sRNA being a relatively reliable gene in MDDCs infected with HIVBaL, but unreliable in other HIV infections and ... strains Towne and Toledo, VZV strain Schenke and HIV-1 strains BaL, NL4.3 and RFW were propagated according to standard protocols [16-20] The cell types infected, MOI, time of infection and percentage ... determining the suitability of 10 commonly used reference genes in the context of infection with various strains of HIV-1, HSV-1, CMV and VZV in a range of both primary and cultured cell lines using...
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Báo cáo sinh học: " Reference gene selection for quantitative real-time PCR analysis in virus infected cells: SARS corona virus, Yellow fever virus, Human Herpesvirus-6, Camelpox virus and Cytomegalovirus infections" doc
Ngày tải lên :
18/06/2014, 22:20
... BestKeeper indices; even actin was included into our gene panel These findings demonstrate the usefulness of analysing a wide variety of reference gene candidates The inconsistent data regarding to ... highest for Act in of viruses, indicating that Act is no reliable reference gene in this setting In contrast, TBP and PPI displayed the highest expressional stability for of viruses To find a general ... find putative candidates that can be shortlisted when setting up a new experimental design Therefore, we determined the expression of previously tested reference genes in a setting of virus infected...
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Báo cáo hóa học: " Determination of suitable housekeeping genes for normalisation of quantitative real time PCR analysis of cells infected with human immunodeficiency virus and herpes viruses" pot
Ngày tải lên :
20/06/2014, 01:20
... reflecting variability in both virus strain and cell type Notable differences included 18sRNA being a relatively reliable gene in MDDCs infected with HIVBaL, but unreliable in other HIV infections and ... strains Towne and Toledo, VZV strain Schenke and HIV-1 strains BaL, NL4.3 and RFW were propagated according to standard protocols [16-20] The cell types infected, MOI, time of infection and percentage ... determining the suitability of 10 commonly used reference genes in the context of infection with various strains of HIV-1, HSV-1, CMV and VZV in a range of both primary and cultured cell lines using...
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báo cáo hóa học:" Reference gene selection for quantitative real-time PCR analysis in virus infected cells: SARS corona virus, Yellow fever virus, Human Herpesvirus-6, Camelpox virus and Cytomegalovirus infections" doc
Ngày tải lên :
20/06/2014, 04:20
... BestKeeper indices; even actin was included into our gene panel These findings demonstrate the usefulness of analysing a wide variety of reference gene candidates The inconsistent data regarding to ... highest for Act in of viruses, indicating that Act is no reliable reference gene in this setting In contrast, TBP and PPI displayed the highest expressional stability for of viruses To find a general ... find putative candidates that can be shortlisted when setting up a new experimental design Therefore, we determined the expression of previously tested reference genes in a setting of virus infected...
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báo cáo khoa học: " Selection of reference genes for quantitative real-time PCR expression studies in the apomictic and sexual grass Brachiaria brizantha" ppt
Ngày tải lên :
12/08/2014, 03:20
... and sexual reproduction within Brachiaria makes it an interesting system for understanding the molecular pathways involved in both modes of reproduction The identification of genes involved in ... controlling the expression of this trait and engineering crops with higher productivity and a reduced risk of gene transfer One way of comparing these different molecular pathways is by comparing ... ones are those constitutively expressed and involved in basic cellular processes, such as protein and sugar metabolism and cell structure [18,20-22] A largescale comparative analysis of the most...
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báo cáo khoa học: " Validation of reference genes for quantitative real-time PCR during leaf and flower development in Petunia hybrida" pot
Ngày tải lên :
12/08/2014, 03:21
... gene to use in normalization in both lines In contrast, the suggested genes did not coincide and were EF1a and SAND in Mitchell, whilst CYP and RAN1 were the genes of choice in V30 In conclusion, ... PCR; RAN1: GTP-binding nuclear protein; RG: reference genes; RPS13: Ribosomal protein S13; RQ: Relative quantity; SAND: SAND family protein; TUB: b-Tubulin chain; UBQ: Polyubiquitin Acknowledgements ... evaluating EF1a as the most reliable gene in line Mitchell but differed in line V30, recommending ACT as the best candidate EF1a suitability has been confirmed in potato during biotic and abiotic...
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Báo cáo khoa học: "Detection of anatid herpesvirus 1 gC gene by TaqMan™ fluorescent quantitative real-time PCR with specific primers and probe" pps
Ngày tải lên :
12/08/2014, 04:21
... postinoculation in AHV-1 Cha strain-infected ducks The concentration of nucleic acid in DNA vaccine-inoculated ducks maintained 107 copies/g level at weeks postinoculation The copies of the liver, ... vaccine (AHV-1 Cha) strain virus and AHV1 virulent (AHV-1 Chv) strain virus were found positive for AHV-1 by the established FQ-PCR assay, while the bacteria, remaining viruses including negative ... Rodriguez J: Development of a DIVA (Differentiating Infected from Vaccinated Animals) strategy using a vaccine containing a heterologous neuraminidase for the control of avian influenza Avian Pathol...
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Tài liệu Comparison of lung cancer cell lines representing four histopathological subtypes with gene expression profiling using quantitative real-time PCR pptx
Ngày tải lên :
15/02/2014, 04:20
... distinct, small negative value in PC3 Two SQ cell lines, EBC-1 and LK-2, were close in PC1, PC2, and PC3, whereas one SQ cell line, LC-1/sq, showed a distinct, large positive value in PC1 and PC3 Two ... Two close AD cell lines and two close SQ cell lines were different in PC3 with a positive value in AD and a negative value in SQ ABC-1 (AD) and LC-1/sq (SQ) were also separated in different directions ... growth [25] STMN1 and S100P may work in SC cell lines and AD cell lines, respectively, in this study The third connection including TGM2, IL1B, and PLAU was suggested to be tumor invasion It was...
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Báo cáo hóa học: " A quantitative real time PCR method to analyze T cell receptor Vb subgroup expansion by staphylococcal superantigens" doc
Ngày tải lên :
18/06/2014, 16:20
... phospholipase C and phosphokinase C pathways [10], leading to a massive production of proinflammatory cytokines including interleukin-2 and interferon-g [11], resulting in extensive proliferation ... (pMIN121) and S aureus RN4220 (pCE107), respectively, using preparative isoelectric focusing as described previously [17-19] Other toxins used in this study were produced in recombinant form using ... determined by analyzing melting curves and sequencing amplified PCR products Melting curve analysis and sequencing amplified PCR products of reactions for, Cb and Vb subgroups consisting of a single...
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Báo cáo y học: "Endothelial Cytomegalovirus infection monitored by quantitative real-time PCR in critically ill patients" pps
Ngày tải lên :
14/08/2014, 07:21
... 30 9,130 Discharged from ICU 141 63,400 Died in ICU 133 130,000 Discharged from ICU 105 137,000 Died in ICU ICU stay (days) Viremia peak is expressed in copies/ml DBSA, deep burn surface area; ... http://ccforum.com/content/15/2/412 Page of Figure Cytomegalovirus plasma load measurements during ICU stay of four severe burn patients Table Patient characteristics Patient Age (years) TBSA...
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Báo cáo sinh học: "Quantitative real-time PCR study on persistence of pDNA vaccine pVax-Hsp60 TM814 in beef muscles" pptx
Ngày tải lên :
14/08/2014, 19:22
... Vaccines and Therapy 2008, 6:11 Background DNA-based vaccines represent a new and rapidly progressing area in vaccinology So far, plasmid DNA (pDNA) vaccines have been reported to induce protective ... parts of DNA vaccinated livestock, mainly in muscles Nowadays, there are no definitive guidelines available to approve usage of DNA vaccines in food- producing animals In this work, the QRTPCR ... Sharpe SA, Powell LAJ, McLoughlin L, Crome SJ: Biodistribution and persistence of an MVAvectored candidate HIV vaccine in SIV-infected rhesus macaques and SCID mice Vaccine 2005, 23:1507-1514 Raska...
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Quantitative real time PCR
Ngày tải lên :
05/09/2015, 10:35
... contained herein Printed on acid-free paper Humana Press is a brand of Springer Springer is part of Springer Science+Business Media (www.springer.com) Preface From the first report describing real-time ... quantitative real-time PCR) guidelines were published in 2009 with the twin aims of providing a blueprint for good real-time quantitative polymerase chain reaction (qPCR) assay design and encouraging ... sensitivity, and specificity of each individual assay be determined This information should be verified by the investigator for the actual assay that is being reported using the conditions and personnel...
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Báo cáo y học: "Expression analysis of three isoforms of hyaluronan synthase and hyaluronidase in the synovium of knees in osteoarthritis and rheumatoid arthritis by quantitative real-time reverse transcriptase polymerase chain reaction" potx
Ngày tải lên :
09/08/2014, 01:24
... the message expression of hyaluronidase-2 in the synovium of OA and RA was approximately four times that in the control synovium This finding suggests that in OA and RA, the protein expression ... grade joint space narrowing combined with osteophytes, or of grade or joint space narrowing Control synovial samples were obtained from donors who had no intra-articular pathologic findings under ... relative expression level = 2-∆average∆CT Figure osteoarthritis hyaluronidase-1, -2, and -3 in the synovium synthase-1, 2, and -3 and (OA) levels of the messages (RA) Relative expressionand rheumatoid...
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Báo cáo hóa học: "Detection of carcinoembryonic antigen messenger RNA in blood using quantitative real-time reverse transcriptase-polymerase chain reaction to predict recurrence of gastric adenocarcinoma" potx
Ngày tải lên :
18/06/2014, 16:20
... analysis HP and XRH conceived of the study, and participated in its design and coordination and helped to draft the manuscript All authors read and approved the final manuscript Competing interests ... differentiate baseline gene expression levels in normal tissues from increased gene expression levels in cancer, thereby increasing the concern for false-positive results [17] In our study, realtime ... survival The 5-year overall survival was 58.9% and 3-year disease free survival was 63.9% Both univariate and multivariate analyses were used to evaluate factors relating to diseasefree survival...
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Báo cáo sinh học: " Detection, quantification and genotyping of Herpes Simplex Virus in cervicovaginal secretions by real-time PCR: a cross sectional survey" potx
Ngày tải lên :
19/06/2014, 08:20
... relating to sensitivity of the HSV-1 and -2 probe relative binding In a single step multiplex assay mutations in the probe binding area are reported to lead to a loss in sensitivity and error in ... no mutations in the probe binding area The remaining samples resulted in poor quality sequencing reactions and the results were not interpretable Discussion Diagnostic methods, ranging from traditional ... such interventions demands rapid, efficient, reliable and type specific assays These assays can serve as biological endpoints in deciding when to administer intervention, monitoring the effectiveness...
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Báo cáo sinh học: " Comparison of real-time PCR and hemagglutination assay for quantitation of human polyomavirus JC" pptx
Ngày tải lên :
19/06/2014, 08:20
... HIV infection and about 3–5% of AIDS patients develop this disease [6,7] Factors influencing PML pathogenesis, including modes of JCV transmission, its dissemination from site(s) of initial infection, ... containing 64 HAU to 0.001 HAU of JCV DNA was extracted using a QIAprep Miniprep Spin Kit and eluted in 50 µl of the elution buffer Two µl of the 1:10 diluted template DNA was used for PCR in a final ... 3:3 and inter-run coefficients of variation for the standard curve varied from 0.06% to 4.8% and 2.6% to 5.2%, respectively, and thus appeared to be consistent for detecting and quantitating...
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báo cáo hóa học:" Detection, quantification and genotyping of Herpes Simplex Virus in cervicovaginal secretions by real-time PCR: a cross sectional survey" pdf
Ngày tải lên :
20/06/2014, 04:20
... relating to sensitivity of the HSV-1 and -2 probe relative binding In a single step multiplex assay mutations in the probe binding area are reported to lead to a loss in sensitivity and error in ... no mutations in the probe binding area The remaining samples resulted in poor quality sequencing reactions and the results were not interpretable Discussion Diagnostic methods, ranging from traditional ... such interventions demands rapid, efficient, reliable and type specific assays These assays can serve as biological endpoints in deciding when to administer intervention, monitoring the effectiveness...
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báo cáo hóa học:"Comparison of real-time PCR and hemagglutination assay for quantitation of human polyomavirus JC" potx
Ngày tải lên :
20/06/2014, 04:20
... HIV infection and about 3–5% of AIDS patients develop this disease [6,7] Factors influencing PML pathogenesis, including modes of JCV transmission, its dissemination from site(s) of initial infection, ... containing 64 HAU to 0.001 HAU of JCV DNA was extracted using a QIAprep Miniprep Spin Kit and eluted in 50 µl of the elution buffer Two µl of the 1:10 diluted template DNA was used for PCR in a final ... 3:3 and inter-run coefficients of variation for the standard curve varied from 0.06% to 4.8% and 2.6% to 5.2%, respectively, and thus appeared to be consistent for detecting and quantitating...
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Báo cáo khoa học: "A multiplex real-time PCR for differential detection and quantification of Salmonella spp., Salmonella enterica serovar Typhimurium and Enteritidis in meats" pps
Ngày tải lên :
07/08/2014, 23:22
... 128 bacterial strains including 110 Salmonella strains (51 S Typhimurium strains, 12 S Enteritidis strains and 47 other Salmonella serotype strains) and 18 nonSalmonella strains were tested by ... Enteritidis in beef, and 4.57 ± 0.02 and 2.26 ± 0.05 log10 CFU/ml for S Typhimurium and S Enteritidis in pork (Figs and 4) Our results indicated that the QIAamp DNA Mini Kit was the most effective in ... methods It was indicated that the QIAamp DNA Mini Kit may be the most efficient in harvesting bacterial DNA and reducing the remaining PCR inhibitors Therefore, the QIAamp DNA Mini Kit was utilized...
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