Ngày tải lên :
21/02/2014, 01:21
... was performed in a solution of 25 lL containing, 50 mM Tris/HCl pH 8.3, 75 mM KCl, mM MgCl2, 10 mM dithiothreitol, 0.2 mM of each dNTP, 0.1 lg of oligo dT, 200 units of M-MLV reverse transcriptase ... 25 lL of a reaction mixture containing, lL of the reverse transcribed cDNA, 20 mM Tris/HCl pH 8.3, 50 mM KCl, 1.5 mM MgCl2, 2.5 U of Taq polymerase (Life Technologies, Paisley, UK), 0.2 mM of each ... Briefly, 90 lL of nuclei suspension were added with 100 lL of · reaction buffer (10 mM Tris/HCl, pH 7.5, mM MgCl2, 0.3M KCl, mM dithiothreitol, mM each of ATP, GTP, and CTP), and lL of [a-32P]UTP...