case study targeted high throughput sequencing of mitochondrial genomes from extinct cave bears
Báo cáo y học: "miRTRAP, a computational method for the systematic identification of miRNAs from high throughput sequencing data" pptx
... products Nearly half of the expanded collection of miR genes (172 out of 380) identified in this study produce moRs from at least one side of the hairpin Indeed, the presence of moRs lends support ... biological significance of a single locus producing miRs from both directions is unclear In the case of Drosophila iab-4/iab8, iab-8 is produced from the opposite side of iab-4*; thus, its sequence ... coverage of the Drosophila libraries [17] miRTRAP performs better when more sequencing data are available However, this is not a concern given the rapid advances of high- throughput sequencing...
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![bioinformatics for high throughput sequencing [electronic resource]](https://media.store123doc.com/images/document/14/y/gu/small_Yb8L87hXir.jpg)
bioinformatics for high throughput sequencing [electronic resource]
... high- throughput sequencing: preparation, immobilization, and sequencing Preparation of the sample for highthroughput sequencing involves random fragmentation of the genomic DNA and addition of ... In some cases, sequencing has replaced traditional Introduction approaches such as microarrays, furthermore offering finer outcomes A review of each of the applications of high- throughput sequencing ... have elapsed since the development of modern DNA sequencing till today’s apogee of high- throughput sequencing During that time, starting from the sequencing of the first small phage genome (5,386...
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Báo cáo sinh học: "Generalized Models for high-throughput analysis of uncer- tain nonlinear systems" doc
... Purpose — Describe a high- throughput method for the analysis of uncertain models, e.g in biological research Methods — Generalized modeling for conceptual analysis of large classes of models Results ... the early stages of exploration of a system, so that insights from modeling can feed into experimental set ups Method Here we present the approach of generalized modeling The idea of this approach ... dynamics of uncertain networks are revealed as a function of intuitive parameters Conclusions — Generalized modeling easily scales to very large networks Keywords — Generalized modeling; high- throughput...
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Báo cáo hóa học: " Generalized Models for high-throughput analysis of uncer- tain nonlinear systems" pdf
... Purpose — Describe a high- throughput method for the analysis of uncertain models, e.g in biological research Methods — Generalized modeling for conceptual analysis of large classes of models Results ... the early stages of exploration of a system, so that insights from modeling can feed into experimental set ups Method Here we present the approach of generalized modeling The idea of this approach ... dynamics of uncertain networks are revealed as a function of intuitive parameters Conclusions — Generalized modeling easily scales to very large networks Keywords — Generalized modeling; high- throughput...
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Báo cáo toán học: " Generalized Models for high-throughput analysis of uncer- tain nonlinear systems" ppt
... Purpose — Describe a high- throughput method for the analysis of uncertain models, e.g in biological research Methods — Generalized modeling for conceptual analysis of large classes of models Results ... the early stages of exploration of a system, so that insights from modeling can feed into experimental set ups Method Here we present the approach of generalized modeling The idea of this approach ... dynamics of uncertain networks are revealed as a function of intuitive parameters Conclusions — Generalized modeling easily scales to very large networks Keywords — Generalized modeling; high- throughput...
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Báo cáo khoa học: "High throughput evaluation of gamma-H2AX Dane Avondoglio1, Tamalee Scott1, Whoon Jong Kil, Mary " ppsx
... treatment per experiment (B) Plot of the linear dynamic range of the immunofluorescent staining assay (C) Plot of the linear dynamic range of the MSD Assay Page of (page number not for citation ... number of DNA DSBs produced after irradiation, the kinetics of the Figure Comparison of foci versus MSD for the evaluation of DNA-DSB repair Comparison of foci versus MSD for the evaluation of DNA-DSB ... figure from one of three independent experiments As shown, exposures higher than Gy result in foci saturation, reducing the useful range of the assay to doses less than Gy As a measure of linearity,...
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Báo cáo y học: "Targeted next-generation sequencing of a cancer transcriptome enhances detection of sequence variants and novel fusion transcript" ppt
... sequences from genes of known relevance to cancer, targeted RNA-Seq makes possible the identification of translocations for any number of targeted genes in a single experiment In addition, oncogenes often ... genomic DNA, typically for the purpose of enriching exonic DNA of interest Although targeted sequencing of genomic DNA facilitates mutation-discovery/ profiling, it is unable to interrogate the ... (Figure 2) In addition, targeted RNA-Seq can achieve higher increases in coverage for a subset of targeted transcripts, depending on the number of unique baits designed If coverage of lower abundance...
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báo cáo khoa học: " High-throughput analysis of chromosome translocations and other genome rearrangements in epithelial cancers" pdf
... per case Most of the fusion genes were of unknown function but several involved known or likely cancer genes, such as ETV6, which is a known target of translocations and encodes a member of the ... parallel paired end sequencing will remain a research tool, but the basic cost of an analysis like that of Stephens et al [5] is already down to a few thousands of euros per case, so it is conceivable ... Development of personalized tumor biomarkers using massively parallel sequencing Sci Transl Med 2010, 2:20ra14 doi:10.1186/gm140 Cite this article as: Newman S, Edwards PAW: High- throughput analysis of...
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báo cáo khoa học: " High-throughput sequencing identifies STAT3 as the DNA-associated factor for p53-NF-κB-complex-dependent gene expression in human heart failure" pps
... handling of reads >32 bp The length and abundance of ChIP fragments were modeled from sequencing reads using Model-Based Analysis of ChIP-Seq (MACS) with model fold at 100 and P-value cutoff at ... phospho-p53ser20 (Cell Signaling), as previously described [15] ChIP-Seq High- throughput sequencing of the sequential-ChIP fragments from human hearts was performed using Illumina Genome Analyser by ... with UK Home Office regulations Primary cardiac fibroblasts, immortalized RelA-/- mouse embryo fibroblast (MEF) cells (from Professor R Hay, University of Dundee), p53-/- MEF cells (from Dr G Lozano,...
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Báo cáo y học: "Establishment of a novel CCR5 and CXCR4 expressing line which is highly sensitive to HIV and suitable for high-throughput evaluation of CCR5 and CXCR4 antagonists" pdf
... ELISA days after infection of cells The coreceptor use of each isolate is shown between brackets The data of one representative experiment out of two are presented Page of 13 (page number not for ... Establishment of the U87.CD4.CCR5.CXCR4 cell line Because of its complete lack of endogenous CCR5 or CXCR4 expression, we used the U87.CD4 cell line as a starting point to create a cell line highly ... fluorescence intensity of the cells (y-axis) as function of time (x-axis) using the FLIPR Each data point represents the average value of the fluorescence measured in quadruplicate The data of one representative...
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Báo cáo y học: "High-throughput analysis of spatio-temporal dynamics in Dictyostelium" docx
... Snapshots of movies from wild-type AX4 cells at representative stages of development Images were captured every 40 sec from each well for 10.5 h after plating for a total of 800 frames Later stages of ... shown ofvideo2a.filemovie 13athe view data to images of moviesfound inin A time-lapserawfile of2 a 3b-i of otherthe online the datathe hours Additionalshowndataframe-subtractedof1development of for ... 10.5 h, a time course of strength of the signal oscillating at the specified periodicity s was obtained from averaged wavelet transformations of pixel intensities as a function of time (see main...
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Báo cáo y học: "Genomics - from Neanderthals to high-throughput sequencing" ppsx
... duration of the study, and this technique is being applied to identifying VDJ profiles in T-cells and B-cells associated with disease and vaccination Making sense of SNPs The most recent inrush of ... has contributed genomic information from mammoths, cave bears and Neanderthals The small amount of data available so far indicate that Neanderthals diverged from modern humans (Homo sapiens) 400,000 ... practical front, it may also highlight the need for a more cautious approach to the common practice of masking and subsequent removal of large numbers of repeated elements from genomic analyses Volume...
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Methods for DNA copy number variation analysis using high throughput sequencing
... Principles of the Sanger sequencing technology 21 1.6 Principles of pyrosequencing 24 1.7 Principles of the 454 pyrosequencer 25 1.8 Principles of sequencing- by-synthesis ... Development of DNA Sequencing Technologies The rapid development of sequencing technologies is continuously increasing the speed and decreasing the cost of DNA sequencing The next-generation sequencing, ... in high- throughput identification of SNPs On a SNP genotyping array, each SNP allele is represented by matched and mismatched groups of probes approximately 25 bp long Due to the high density of...
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Development and application of advanced proteomic techniques for high throughput identification of proteins
... the study of the complete set of genetic information of an organism (Mckusick, 1997), which encompasses mapping, sequencing and analysis of the whole genome of an organism The significance of ... quadrupole/time -of- flight (Qq-TOF), time -of- flight/ time-offlight (TOF/TOF) and quadrupole/ion-trap mass spectrometers are mainstream instruments for protein sequencing These sequencing tools, ... Tandem Affinity Purification TOF Time of Flight TOF/TOF Time -of- Flight/Time -of- Flight UV Ultraviolet Y2H Yeast Two-Hybrid xvi Chapter Introduction The complete sequence of the human genome (Lander...
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Báo cáo khoa học: Purification, characterization, cDNA cloning and nucleotide sequencing of a cellulase from the yellow-spotted longicorn beetle, Psacothea hilaris ppt
... different primers designed from the cDNA sequence resulted in the amplification of bands in each case Sequencing of these bands showed that they matched the sequence of the cellulase cDNA completely; ... cellulase in the larval gut of P hilaris The sensitivity of the activity staining used is high, and CMCase activity has been detectable with lL of 1000 times diluted gut juice of P hilaris larvae Therefore, ... ORF consisted of a protein of 325 amino acids The molecular mass of P hilaris Fig Comparison of amino-acid sequences for potential catalytic proton donor and nucleophile regions of P hilaris cellulase...
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Organizing pairwork and groupwork in the context of high school classrooms at pham van nghi upper secondary school, nam dinh province: A case study
... Rationale Aims of study Scope of the study .8 Research questions of the study .9 Methods of study Design of study Part ... students at PVN upper secondary school? Methods of study To realize the aims of the study, a case study was used.The theoretical background of the study comes from many published books written by different ... CONTEXT OF HIGH SCHOOL CLASSROOMS AT PHAM VAN NGHI UPPER SECONDARY SCHOOL, NAM DINH PROVINCE: A CASE STUDY Is the result of my own research for the Degree of Master of Arts at College of Foreign...
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Using compensation strategies in listening for 10th form students a case study at the high school for gifted students of vinh university
... form students: A case study at the high school for gifted students of Vinh University” is submitted in fulfillment of the requirements for the degree of Master of Arts is the result of my own work ... 1.2 Aim of the study 1.3 Research questions .7 1.4 Scope of the study 1.5 Significance of the study 1.6 Organization of the study CHAPTER ... the problem leading to the study, aims of the study, research questions, scope, and organization of the study In the second chapter, the theoretical background of the study will be presented in...
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Changes in the Economic Value of Variable Generation at High Penetration Levels: A Pilot Case Study of California pot
... load factor of CCGTs in a case with an increasing penetration of a flat block of power Hence, cases with high VG penetration and even the case with high penetrations of a flat block of power increasingly ... to high penetration levels 10 Note that the exact penetration level used to describe each of the cases varies from the case title For example, the actual penetration of PV in the “30% PV” case ... forecast for VG (in the case of wind, PV, and CSP0 ) or historic hourly load (in the case of CSP6 ) The decision to use a fraction of the historic hourly load in the case of CSP6 was based on early...
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Báo cáo y học: " Dynamics of mitochondrial heteroplasmy in three families investigated via a repeatable re-sequencing study" doc
... http://genomebiology.com/2011/12/6/R59 Page of 16 Figure Individuals and samples used in the study Numbers in parenthesis are the age of each individual; the number at the bottom of each table is count of sequencing reads ... heteroplasmy from high- throughput sequencing of complete human mitochondrial DNA genomes Am J Hum Genet 2011, 87:237-249 18 Galaxy page describing analysis of mitochondrial heteroplasmy [http:// ... estimation by deep sequencing of reduced representation libraries Nat Methods 2008, 5:247-252 40 Bansal V: A statistical method for the detection of variants from nextgeneration resequencing of DNA pools...
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High achieving pupils’ experiences of Assessment for Learning in a mainstream junior school: a qualitative case study drawing on perspectives from psychoanalytic theories
... of an embedded exploratory case study (Yin, 2003) where a cohort of fifteen children in a year group was the case, with the individual pupils being the units of analysis An advantage of the case ... interpretation of the responses of the pupils in my study A consideration of Winnicott’s perspectives on the psychological and emotional impact of AfL on the pupils is given throughout the second part of ... from Graham and Fitzgerald (2010) Theoretical positioning Essentially, two theoretical positions underpin the concept of pupil voice – that of the nature of childhood and that of the rights of...
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Tìm thêm: xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn xác định thời lượng học về mặt lí thuyết và thực tế điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến dòng điện stato i1 fi p2 thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25