... systolic time andheart rate and expressed in seconds per minute Diastolic time was calculated as the cardiac length (RR interval) minus the duration of systole To define diastolic duration during exercise ... heterogeneous termination LV isovolumic 338 time intervals were found to be increased at the expense of LV diastolic time inheartfailure patients (17,18) Since our findings and other previous ... lack of increase in LV dyssynchrony during dobutamine stress in patients with wide QRS duration (20) In contrast, Kurita et al reported on an increased mechanical dyssynchrony during pacing–induced...
... HERs are commonly activated upon binding of a ligand in their extracellular domain, resulting in dimerization of HERs and triggering the intrinsic tyrosine kinase activity of the receptors responsible ... fusion gene encoding the chimeric protein of caspase-8 and the ligand-binding domain (LBD) of ERα and introduced the gene into breast cancer cell lines (Zhao et al, 2011) Upon administration of ... better understanding of carcinogenesis in diverse tissues, e.g the mammary gland (Johnstone et al, 2002) Both extrinsic and intrinsic stimuli can initiate apoptotic signaling, which involves the...
... HERs are commonly activated upon binding of a ligand in their extracellular domain, resulting in dimerization of HERs and triggering the intrinsic tyrosine kinase activity of the receptors responsible ... fusion gene encoding the chimeric protein of caspase-8 and the ligand-binding domain (LBD) of ERα and introduced the gene into breast cancer cell lines (Zhao et al, 2011) Upon administration of ... better understanding of carcinogenesis in diverse tissues, e.g the mammary gland (Johnstone et al, 2002) Both extrinsic and intrinsic stimuli can initiate apoptotic signaling, which involves the...
... England Journal of Medicine CORRECTION Sudden Deathin Patients with Myocardial Infarction and Left Ventricular Dysfunction, Heart Failure, or Both Sudden Deathin Patients with Myocardial Infarction ... LXRα is expressed predominantly in tissues involved in lipid homeostasis in the liver, intestine, adipose tissue, and macrophages LXRα and LXRβ are equally effective in promoting reverse cholesterol ... reviewed all deaths and episodes of cardiac arrest with resuscitation in a blinded fashion, using source documentation provided by the site investigators Deaths were classified as having cardiovascular...
... required, at least in part, for imatinib-induced apoptosis in BCR–ABL-transformed murine progenitor cells [64] andin BCR–ABL+ K562 CML cells in response to both imatinib and nilotinib [65] INNO-406, ... imatinib therapy have point mutations in the BCR–ABL kinase domain, including the T315I gatekeeper mutation that impairs imatinib binding [58,59] Accordingly, new therapies are being tested and ... [44–46] It is interesting to note that in all cases MEK inhibitor-induced BIM expression alone was not sufficient to induce a dramatic increase incell death; pronounced increases in apoptosis...
... hand, in non-neuronal cells, cytoplasmic retention of ERK1 ⁄ is required for death- associated protein kinase-mediated celldeath [35] Thus, ERK1 ⁄ might promote celldeath depending upon the cell ... it remains to be seen whether ERK5 is also involved in ERK1 ⁄ 2-implicated celldeath paradigms ERK1 ⁄ in cellular models of neuronal death The first evidence for a role of ERK1 ⁄ incelldeath ... of multiple mitogen-activated protein kinases in an oligodendrocyte cell line: role of extracellular signal-regulated kinase in hydrogen peroxide-induced celldeath J Neurochem 72, 112–119 12 Stanciu...
... ANT1-silencing induces glioblastoma celldeath A Fig Analysis of ANT1 and ANT2 expression in nontransfected ADF cells andin ANT1- and ANT2-silenced ADF cells (A) Number of ANT1 and ANT2 transcripts in ... reduction incell viability induced by ANT1 siRNA requires protein synthesis ANT1-silencing induces ADF cell apoptosis and a non-apoptotic celldeath modality similar to paraptosis To gain more insight ... ANT1-silencing induces glioblastoma celldeath A Lena et al Because of their multitask nature, ANTs are involved in several aspects of cell metabolism, as well as incell survival ⁄ death processes, and...
... assessing the sub-G1 population (Figure 4B) In addition, apoptotic cells were also detected by both Annexin V/PI staining and immunofluorescent staining with Hoechst 33342 Annexin V/PI staining ... VX-680 treated cells VX-680 suppresses cell growth and induces cell apoptosis in NB4-R2 cells Next, we studied if VX-680 could suppress proliferation in NB4-R2 cells in vitro NB4-R2 cells were treated ... pAur-A at the activation site and induces monopolar spindle in NB4-R2 cells We studied the inhibition of Aurora kinases in NB4-R2 cells using VX-680 Aur-A activation was inhibited by VX-680 at different...
... matrix shown in C is indicated in D Similar analysis as in A and B was used, but included only MPTP-treated values and assigned value of for no treatment and 10, 30 and 50 for increasing dosage ... to dopaminergic neuronal death by oxidizing dopamine to a reactive dopamine quinone [51], by increasing DNA oxidation [7], or through increased microglial activation leading to chronic inflammation ... of oxidative and inflammatory insults The positive feed back loop may continue until the additional neuronal death (~50% of the remaining of the initial death) combined with the initial loss...
... assessing the sub-G1 population (Figure 4B) In addition, apoptotic cells were also detected by both Annexin V/PI staining and immunofluorescent staining with Hoechst 33342 Annexin V/PI staining ... VX-680 treated cells VX-680 suppresses cell growth and induces cell apoptosis in NB4-R2 cells Next, we studied if VX-680 could suppress proliferation in NB4-R2 cells in vitro NB4-R2 cells were treated ... pAur-A at the activation site and induces monopolar spindle in NB4-R2 cells We studied the inhibition of Aurora kinases in NB4-R2 cells using VX-680 Aur-A activation was inhibited by VX-680 at different...
... perifosine and the prototypic intravenously applicable ErPC3, in preclinical and clinical investigations is based on their ability to induce apoptosis in tumor cells and their ability to increase ... effect of combined treatment with ErPC3 and ionizing radiation PC3 cells (A), DU145 cells (B), and LNCaP cells (C) were treated with increasing concentrations of ErPC3 (1-100 µM) and ionizing radiation ... the ErPC3-induced down-regulation of Mcl-1 and p-Akt may be sufficient to overcome the cellular death threshold and to induce apoptotic death of LNCaP cells [16,36] In PC3-cells, ionizing radiation...
... effective interventions to prevent organ failurein sepsis During sepsis, there is extensive apoptotic death of lymphocytes and gastrointestinal epithelial cells [7] The increased apoptotic death ... these promising findings must be tempered by the new study showing that multiple pathways might be activated in dying cells during sepsis [1] The simple inhibition of a particular celldeath route ... liver injury [9] In addition to apoptosis, certain caspases, such as caspase-1, are involved in cytokine maturation by proteolytically processing the inactive precursors of interleukin (IL)-1β and...
... cullin3 Cylindromatosis cytochrome c death- associated protein-1 death- associated protein kinase DEP domain containing mTOR-interacting protein direct inhibitor of apoptosis-binding protein with ... protects against EBSS-induced celldeathin TSC2-/- cells 89 3.2.5 Nutrients supplementation protects against celldeathin TSC2+/+cells, but enhances celldeathin TSC2-/- cells under ... UVRAG-Bif1-Beclin 1-p150-PI3KC3 (Janku et al., 2011) In contrast, the RUN domain and cysteine-rich domain containing Beclin interacting protein (Rubicon) interacts with UVRAG–Beclin 1–hVps34–p150...
... direct linkage between JNK signaling and the apoptotic cell- death machinery, especially mitochondrial cell death, remains unclear In the present study, we investigated the basis for interaction ... understanding of cell- death events after ionizing irradiation and development of molecular targets for cancer treatment Results To examine the kinetics of the apoptotic celldeath induced by ionizing ... in the regulation of apoptotic celldeathin response to various stimuli To investigate a potential involvement of MAPK in ionizing radiationinduced cell death, we employed specific chemical inhibitors...
... rapidly and extensively induce celldeathin the human breast cancer cell line L56Br-C1 This cell line carries a germ-line mutation (1806 C fi T) in the BRCA1 tumour Fig Propidium iodide-stained ... being involved in DENSPM-induced celldeathin L56Br-C1 cells The Ó FEBS 2002 1038 C Hegardt et al (Eur J Biochem 269) pathway involves a change in mitochondrial transmembrane potential andin ... Correlations between polyamine analogue-induced increases in spermidine/spermine N1-acetyltransferase activity, polyamine pool depletion, and growth inhibition in human melanoma cell lines Cancer Res 51,...
... normal human PBLs and B-lymphoid cells, by inducing mitotic catastrophe and erosion of telomeric DNA selectively in the cancer cells and by inducing cell proliferation in the normal cells Although ... results further imply that Ruta induces death- signaling pathways in human glioma brain cancer cells, both in vivo andin vitro, and survival-signaling pathways in normal B and T lymphocytes Discussion ... cancer cells, by either inducing celldeath or preventing further proliferation; b) to explore the molecular mechanism of celldeath by Ruta + Ca3(PO4)2 treatment of brain cancer cells in vitro; and...
... phosphate buffer (pH 7.8) containing 1.5 mgÆmL)1 nitro-blue tetrazolium and a further 10 in the same buffer containing 28 mM TEMED and 28 lM riboflavine After a brief washing in water, the gel was revealed ... from control cells and GAL1/GAL10-driven Bax-expressing cells cis-Parinaric acid was incorporated in mitochondrial membranes, and fluorescence measurements were carried out as indicated in the methods ... dioctylphtalate on h-Bax-induced celldeath kinetics WtB1 cells were grown in a commercial Yeast Nitrogen Base medium (Difco, h), or in a home-made minimal medium in the absence (j) or in the presence...
... FEBS S Cagnol and J.-C Chambard ERK andcelldeath Table Continued Stimuli inducing celldeath Duration of ERK activation promoting celldeath Human melanoma C8161,WM164 Mel Juso Betulinic acid 96 ... Daniels MA (2009) ERK andcell death: ERK location and T cell selection FEBS J Martin P & Pognonec P (2009) ERK andcell death: cadmium toxicity, sustained ERK activation andcelldeath FEBS J Taylor ... phospholipase A2 activity is induced by CD95 ligand cross-linking in the mouse derived Sertoli cell line TM4 and is required to trigger apoptosis in CD95 bearing cells CellDeath Differ 7, 916–924...
... signaling is important for determining cell fate decisions in a diverse number of organisms andcell types (reviewed in [7,8]) In thymocytes, c-JunNH2-terminal kinase (JNK) [9], p38 [10] and extracellular ... Teixeiro and M A Daniels induction of pERK that is localized to the plasma membrane (Fig 2) On the other hand, positive selecting ligands induce a slow and sustained activation of ERK originating ... LAT In line with this, positive and negative selecting ligands show quantitative differences in total phosphorylation of LAT [16], although the phosphorylation state of individual tyrosines in...
... cadmium-induced ERK activation diminishes cell death, whereas MKP3 exogenous expression diminishes celldeath following cadmium intoxication (P Martin and P Pognonec, unpublished data) This sustained ... in NIH3T3 cells [40] andin astrocytes This increase in turn leads to an increase in ROS and to mitochondrial impairment [41] Pretreatment with a calcium chelator reduced ROS production and increased ... lead to celldeathin certain systems, but that cadmium, in addition to caspase activation, triggers other parallel pathways that also culminate incelldeath This could explain why ERK inhibitors,...