... and light strands of the mtDNA, and contains essential transcription and replication elements The D-loop region is a hot spot for mtDNA alterations and the nucleotide positions 16024±16 324 and ... relationship between ROS and mtDNA mutation rate was assessed using a linear correlation test Results CagA and VacA proteins As shown in Fig 1a and b, the CagA protein (120 kDa) and VacA protein (95 ... activity and expression of base excision repair and mismatch repair are down-regulated both in vitro and in vivo Moreover, Hp induces genomic instability in nuclear CA repeats in mice and in mtDNA...
... (GPx1; Fig and Eqn 4) and peroxiredoxins III and V (PrxIII and PrxV; Fig and Eqns and 6, respectively; [55]) At least seven GPx enzymes have been described to date in mammalian cells [56], and two ... Intracellular generation of reactiveoxygenspecies by mitochondria Biochem Pharmacol 69, 719–723 Andreyev AY, Kushnareva YE & Starkov AA (2005) Mitochondrial metabolism of reactiveoxygenspecies Biochemistry ... apyrimidinic endonuclease APE ⁄ Ref1; SSB and DSB, single-strand break and double-strand break, respectively ase, may aid in the generation of abasic sites and single-strand breaks This model provides a...
... UNIVERSITY GRADUATE SCHOOL Research Integrity and Copyright Disclaimer Title of Thesis/Dissertation: Bone Metabolism: The Role of STAT3 andReactiveOxygenSpecies For the degree of Master of Science ... patience and understanding during my unconventional graduate career, and also my committee members Dr James Marrs and Dr Julie Ji I would like to thank Kevin Zhou for his invaluable help in the lab and ... University, August 2012 Bone Metabolism: The Role of STAT3 andReactiveOxygenSpecies Major Professor: Jiliang Li Signal Transducers and Activators of Transcription (STAT3), a transcription factor...
... mitochondrial respiration by correlating the uncoupling effect induced by these molecules with the level and function of UCP2 and free radical production in macrophages We find that MDP induces reactiveoxygen ... effect of MDP on ROS andreactive nitrogen species production by murine peritoneal macrophages + n + /An PI Fig The percentage of viable, dead and apoptotic cells in treated and untreated cells ... peritoneal macrophages by MDP increased cellular ROS andreactive nitrogen species (RNS) production The increased production of reactivespecies was apparent after h of stimulation Since UCP2...
... regulation and evolution of Nox H Sumimoto Introduction Reactiveoxygenspecies (ROS) are conventionally regarded as inevitable deleterious byproducts of aerobic metabolism On the other hand, there ... region, and NADPH-binding and FADbinding domains in the C-terminal cytoplasmic region (Fig 1A), forming a complete apparatus that transports electrons from NADPH via FAD and two hemes to molecular oxygen ... reduce Fe3+ and Cu2+ for iron and copper uptake, but fail to use molecular oxygen as a substrate [19,20] Among the conserved transmembrane segments of gp91phox ⁄ Nox2, the third and fifth helices...
... Determination of intracellular reactiveoxygenspecies as function of cell density Methods Enzymol 352, 91–100 44 Zuo, L & Clanton, T.L (2002) Detection of reactiveoxygenand nitrogen species in tissues ... Signorile, A., Stahl, W., Vina-Ribes, J & Astley, S.B (2002) Antioxidants, reactiveoxygenand nitrogen species, gene induction and mitochondrial function Mol Aspects Med 23, 209–285 Dunbar, D.R., ... mediated by reactiveoxygenspeciesand p38 MAPK signaling pathway in human HepG2 hepatoma cells Free Radic Biol Med 32, 1132–1142 Gardner, P.R (2002) Aconitase: sensitive target and measure...
... dependency and the tissue specificity of Cygb and Ngb mRNA upregulation upon hypoxia and after reoxygenation In addition, we tried to shed light on the ROS-scavenging function of Ngb and on the ... levels For brain and eyes, there was a gradual increase in the amount of H2O2 after hypoxia and reoxygenation In heart, muscle and liver, the H2O2 level dropped after 24 h of reoxygenation The ... in mice under hypoxic conditions and after reoxygenation Cleaved caspase-3 protein expression in heart and liver under hypoxic (H) conditions (48 h) and after reoxygenation (48R8, 48R24: 48 h of...
... radicals, the term 'reactiveoxygenspecies' (ROS) has prevailed, although ROS can be differentiated into reactive nitrogen speciesand other oxidant species The relationship between ROS and articular ... growth factor isoforms and their receptors are expressed in human osteoarthritic cartilage Am J Pathol 2003, 162:171-181 Henrotin Y, Kurz B, Aigner T: Oxygenandreactiveoxygenspecies in cartilage ... in biochemistry and molecular biology Edited by: Burdon RH Amsterdam: Elsevier; 1991:20 Henrotin YE, Bruckner P, Pujol JP: The role of reactiveoxygenspecies in homeostasis and degradation of...
... R: Reactiveoxygen signaling and abiotic stress Physiol Plant 2008, 133:481-489 38 Van Breusegem F, Bailey-Serres J, Mittler R: Unraveling the tapestry of networks involving reactiveoxygenspecies ... MJ: Singlet oxygen is the major reactiveoxygenspecies involved in photooxidative damage to plants Plant Physiol 2008, 148:960-968 53 Lee KP, Kim C, Landgraf F, Apel K: EXECUTER1- and EXECUTER2dependent ... of hydrogen peroxide and singlet oxygen: its use for the assay of reactiveoxygenspecies produced in vivo Plant Journal 2007, 50:475-487 88 de Torres M, Sanchez P, Fernandez-Delmond I, Grant...
... reductase; MS: Murashige and Skoog; NBT: nitroblue tetrazolium; O2: molecular oxygen; 1O2: singlet oxygen; O2.-: superoxide radical; PSII: photosystem II; ROS: reactiveoxygen species; SOD: superoxide ... Asada K: Production and scavenging of reactiveoxygenspecies in chloroplasts and their functions Plant Physiol 2006, 141(2):391-396 Bowler C, Slooten L, Vandenbranden S, De Rycke R, Botterman J, ... hydrogen peroxide and singlet oxygen: its use for the assay of reactiveoxygenspecies produced in vivo Plant J 2007, 50(3):475-487 Chao L, Zhou QX, Chen S, Cui S, Wang ME: Single and joint stress...
... François Debande and André Théwis for support in animal housing facilities, Michèle Nuttings and Daniel Portetelle for providing monoclonal antibodies and Vincenzo Ciminale for helpful comments and critiques ... BLVinfected and control sheep were cultivated in the absence and the presence of mM VPA (Sigma/Aldrich) and the percentages of apoptotic B cells were determined as described above Analysis of reactiveoxygen ... Venerando A, Cavallari I, Petronilli V, La Rocca N, Marin O, Chieco-Bianchi L, Di Lisa F, D'Agostino DM, Bernardi P, Ciminale V: Modulation of mitochondrial K(+) permeability andreactiveoxygen species...
... expression by reactiveoxygenspecies Proc Natl Acad Sci USA 2003, 100:15035-15040 Hildeman DA, Mitchell T, Teague TK, Hanson P, Day BJ, Kappler J, Marrack PC: Reactiveoxygenspecies regulate ... Brody AR: Asbestos-derived reactiveoxygenspecies activate TGF-beta1 Lab Invest 2004, 84:1013-1023 Hildeman DA: Regulation of T-cell apoptosis by reactiveoxygenspecies Free Radic Biol Med ... of ROS in TCR- and CD28stimulated CD25- T cells were dramatically upregulated and continued to increase at day (Fig 6A,B and Fig 7B) Interestingly, the increase in ROS in TCR- and CD28-stim-...
... prevention and treatment of many diseases [2] Reactiveoxygenspecies (ROS), a group of highly reactive molecules, including singlet oxygen, hydroxyl radicals, superoxide anion, nitric oxide and hydrogen ... production of reactiveoxygenspecies Cancer Res 65, 948–956 12 Pan J, She M, Xu ZX, Sun L & Yeung SC (2005) Farnesyltransferase inhibitors induce DNA damage via reactiveoxygenspecies in human ... translocation and cytochrome c release, the levels of Bax and cytochrome c in the cytosol and mitochondria were examined by western blot analysis (Fig 4) The mitochondrial Bax and cytosol cytochrome...
... procedures) Results are the mean ± standard deviations for three independent experiments 10b and endogenous reactiveoxygenspecies significantly, by 10.382-fold and 3.212-fold, respectively 25 000 ... service to our authors and readers, this journal provides supporting information supplied 10b and endogenous reactiveoxygenspecies by the authors Such materials are peer-reviewed and may be re-organized ... generation in C albicans SC5314 cells without and with 10b and MCZ The concentrations of 10b and MCZ were 1, and lgÆmL)1 ROS levels represent the mean ± standard deviations for three independent experiments...
... alginolyticus cells were performed under exclusion of dioxygen In addition, the effect of enzymes that detoxify reactiveoxygenspecies (superoxide dismutase and catalase) on the Na+-NQR activity during ... anaerobic chamber and flushed with N2 In order to investigate the influence of dioxygen and NADH on the activity of the Na+-NQR, membrane vesicles were prepared (A) in the presence of dioxygen without ... Unemoto, with NADH oxidation and Q reduction activities of 2.45 and 0.99 lmolÆmin)1Æmg)1, respectively [15] Inhibition and uncoupling of the Na+-NQR activity by dioxygen Characterization of redox...
... generation of reactiveoxygenspecies in a nuclear factor kappaB-dependent manner Gastroenterology 2010, 138:2509-2518, 2518 e2501 Mendez-Samperio P, Perez A, Alba L: Reactiveoxygen species- activated ... oxidase pathway and Nrf2 in human tracheal smooth muscle cells J Immunol 2008, 181:5098-5110 Lee HM, Shin DM, Kim KK, Lee JS, Paik TH, Jo EK: Roles of reactiveoxygenspecies in CXCL8 and CCL2 expression ... blunted cytokine (TNF-a and IL-1b) and chemokine (CCL2 and CXCL10) protein production in virus-infected microglial cultures (Figure 4A-D) Viral infection activates p38 and p44/42 (ERK1/2) MAPKs...
... Medicine and Toxicology 2009, 4:17 Figure by neutrophils in contact with PM2.5 In vitro generation of reactiveoxygenand nitrogen species In vitro generation of reactiveoxygenand nitrogen species ... of hypochlorous acid (HOCl) andreactive nitrogen species (RNS) also increases, resulting in the oxidation of tyrosine and nitrite and subsequent formation of tyrosyl and nitrogen dioxide (.NO2) ... mm; PON: Paraoxonase; RNS: Reactive nitrogen species; ROS: Reactiveoxygen species; S: Sulfur; SO2: Sulfur dioxide; SOD: Superoxide dismutase; TBARS: Thiobarbituric acid reactive substances; TNFa:...
... contributions XW and YL designed research and wrote and revised the manuscript; QW performed all research experiments and analyzed data; XLZ assisted with cell death experiment LY and YJZ assisted ... Generation of reactiveoxygenspeciesand induction of apoptosis Journal of carcinogenesis 2009, 8:9 26 Lin Y, Shi R, Wang X, Shen HM: Luteolin, a flavonoid with potential for cancer prevention and therapy ... times and the average is shown in each figure For morphological study of cell death, cells were stained with 50 μg/mL of acridine orange and 50 μg/mL of ethidium bromide and then observed and photographed...
... (PMN); ROS: reactiveoxygenspecies Competing interests The author(s) declare that they have no competing interests Authors' contributions All authors participated in study design and read and approved ... tissue repair by submandibular gland factors Immunol Today 1994, 15(11):527-532 Rougeot C, Rosinski-Chupin I, Mathison R, Rougeon F: Rodent submandibular gland peptide hormones and other biologically ... ± 10.2 and 130.0 ± 16.6 MFI for background and PMA-stimulated cells, respectively 0.0 BSA feG OA OA & feG Figure Leukocyte Counts Leukocyte Counts Total leukocyte numbers and the number and percent...
... glutathione, and DNA injury Besides oxygen- based radicals, there are also reactive nitrogen species such as nitric oxide (NO) and nitrogen dioxide (NO2), sulfur based molecules such as thinyl (RS) and ... Nitric oxide, superoxide, and peroxynitrite: the good, the bad, and the ugly Am J Physiol 1996, 40:C1424-C1437 Turrens JF: Mitochondrial formation of reactiveoxygenspecies J Physiol 2003, 552:335-344 ... calcium handling, which is essential for intracellular signaling Lipid peroxides can also damage DNA and proteins Attack of DNA by ROS results in DNA strand breaks As with lipids, O2•– and H2O2...