capsid assembly and maturation
Báo cáo y học: "HIV-1 Capsid Assembly Inhibitor (CAI) Peptide: Structural Preferences and Delivery into Human Embryonic Lung Cells and Lymphocyte" pptx
... during the process of viral maturation The proteins are termed: matrix p17 (MA), nucleocapsid p9 (NC), p4 and capsid p24 (CA) and SP1 and SP2 respectively [9] The assembly of Gag proteins into ... the CAICTRL-BioShuttle, and and the BioShuttle connected to the reverse form of the CAI-inhibitor and the control, respectively Vertically: CAI-Inhibitor, scrambled control and their corresponding ... proteins and their fragments [20, 21] At 10% TFE the CAI inhibitor (figure 4) shows about 50% regular β-strands, whereas the two control peptides contain high levels of coil and turn and a relatively...
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Báo cáo y học: "Characterization of the invariable residue 51 mutations of human immunodeficiency virus type 1 capsid protein on in vitro CA assembly and infectivity" docx
... essential for CAp24 tube formation in vitro, and for HIV-1 replication and capsid formation in cultured virus [6] During proteolysis of the Pr55Gag and maturation of CAp24, the NTD of CAp24 refolds ... proper capsid formation and subsequent replication of the virus, we extended the above findings and examined amino acid substitutions of this invariable residue to asparagine, glutamate, and glutamine ... cell (A and B) and viral lysates (C) were then separated by SDS-PAGE, transferred onto a nitrocellulose membrane and detected with a rabbit anti-HIV glycoprotein (A), a pool of anti-CAp24 and anti-calnexin...
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Báo cáo y học: "Mutation in the loop C-terminal to the cyclophilin A binding site of HIV-1 capsid protein disrupts proper virus assembly and infectivity" ppt
... and Western blot analysis of capsid mutants Structural view and Western blot analysis of capsid mutants A close view of the structure of the cyclophilin A binding loop in the N-terminal (A) and ... immunodeficiency virus type capsid protein affect virus assembly and release J Gen Virol 2004, 85(Pt 10):2903-2913 21 22 23 24 25 Wang CT, Barklis E: Assembly, processing, and infectivity of human ... centrifugation, washed and resuspended in ml complete RPMI medium per well The infections were performed in triplicates and supernatants were collected at days 1, 4, 8, 12 and 16 post-infection and tested...
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Self-Assembly and Nanotechnology: A Force Balance Approach
... APPROACH TO SELF -ASSEMBLY UA,P(x) and UR,P(x) represent the attractive and repulsive potentials for the selfassembly of primary building units, respectively UA,S(x) and UR,S(x), and UA,T(x) and UR,T(x) ... IMPLICATIONS OF SELF -ASSEMBLY FOR NANOTECHNOLOGY 8.1 General Concepts and Approach to Nanotechnology 8.2 Self -Assembly and Nanotechnology Share the Same Building Units 8.3 Self -Assembly and Nanotechnology ... 1997 Bergström, 1997 Hough and White, 1980 Hough and White, 1980 Hough and White, 1980 Visser, 1972 Visser, 1972 Hough and White, 1980 Hough and White, 1980 Hough and White, 1980 Visser, 1972...
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The 8051 microcontroller and embedded systems using assembly and c 2nd ed
... Logic Instructions And Programs 8051 Programming in C 8051 Hardware Connection and Hex File 8051 Timer/Counter Programming in Assembly and C 8051 Serial Port Programming in Assembly and C Interrupts ... Microcontroller and Embedded Systems: Using Assembly and C Mazidi, Mazidi and McKinlay Chung-Ping Young 楊中平 Home Automation, Networking, and Entertainment Lab Dept of Computer Science and Information ... Microcontroller and Embedded Systems: Using Assembly and C Mazidi, Mazidi and McKinlay Chung-Ping Young 楊中平 Home Automation, Networking, and Entertainment Lab Dept of Computer Science and Information...
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Tài liệu Báo cáo khoa học: The capsid protein of human immunodeficiency virus: designing inhibitors of capsid assembly ppt
... immature capsid- like particle assembly in vitro and budding of immature-like capsids The results obtained by Bartonova et al [30] pinpoint an indirect (allosteric) effect of CAI binding on the assembly ... immature capsid lattice, but a direct binding effect in the conserved CTD binding pocket during mature assembly On the other hand, mutations at Tyr169 and Leu211 not yield mature-like particles and ... mutants differ from that of wild-type, and those of the assembly- competent mutants E187A and N183A: the conformations of the two assembly- incompetent mutants (Y169A and L211A) in the absence of CAI...
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Tài liệu Báo cáo khoa học: Tryptophan tryptophylquinone cofactor biogenesis in the aromatic amine dehydrogenase of Alcaligenes faecalis Cofactor assembly and catalytic properties of recombinant enzyme expressed in Paracoccus denitrificans pptx
... orf-4 and hemE) all putatively transcribed in the same direction [7] The second and fifth genes (aauA and aauB) encode the large and small subunits of AADH, respectively The genes aauD and aauE ... cells and media The bacterial strains and plasmids used in this study are listed in Table For strain stocks and DNA isolation, E coli and A faecalis were cultured with Luria–Bertani media at 37 and ... sequence (accession AF302652), shown in emboldened and underlined text, and are as follows: T835 is now C835, C836 is now T836 and an extra T between A1376 and T1377 Underlined amino acid residues denote...
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Báo cáo khoa học: Assembly and molecular mode of action of the Helicobacter pylori Cag type IV secretion apparatus doc
... lack the cagPAI and are less virulent The Cag secretion system is responsible for the induction of a pronounced pro-inflammatory response in vitro and in vivo, and the translocation and subsequent ... cagPAI and the properties of both conserved and unique components of the Cag-T4SS, together with potential implications for the current understanding of its mode of action The cagPAI and the ... review by Terradot and Waksman [30] This core complex consists of 14 monomers each of the VirB7, VirB9 and VirB10 homologues and does not require other secretion system components for assembly It is...
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Báo cáo khoa học: The Vps4 C-terminal helix is a critical determinant for assembly and ATPase activity and has elements conserved in other members of the meiotic clade of AAA ATPases pdf
... and a Vps4p protein containing an intact C-terminal helix Based on our experimental data and bioinformatic analysis, we propose a model for the role of the C-terminal helix in Vps4 assembly and ... ATPase domain and C-terminal helix [36] showing the location of residues that were mutated The TRP, RDF and DEL sequences are shown in green, dark blue and red, respectively The b domain and C-terminal ... wild-type Vps4p and inhibit its function in vivo One possible explanation for the difference between our in vivo and in vitro findings is that in vivo Vta1p may allow assembly of otherwise assembly- incompetent...
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Báo cáo khoa học: The 2-oxoacid dehydrogenase multi-enzyme complex of the archaeon Thermoplasma acidophilum ) recombinant expression, assembly and characterization docx
... 2-oxoacids and 2-Oxoacid dehydrogenase complex from the Archaea pyruvate [14] In the current paper, we report the cloning and expression of the E2 and E3 genes of the same operon from T acidophilum, and ... restriction endonuclease digestion for E2, and NheI ⁄ EcoRI digestion for E3, and purified by gel electrophoresis and gel extraction as already described E2 and E3 genes were separately ligated into ... using primers that engineered restriction sites into the 5¢- and 3¢ ends of the gene products: NdeI and XhoI for the E2 gene, and NheI and EcoRI for E3 Oligonucleotides were as follows (restriction...
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Báo cáo khoa học: Mechanisms of amyloid fibril self-assembly and inhibition Model short peptides as a key research tool pptx
... understanding this life-threatening process Extremely short aromatic peptides form amyloid fibrils readily and efficiently The importance of aromatic moieties in the molecular recognition and self -assembly ... Themes and variations Curr Opin Struct Biol 10, 60–68 Serpell LC (2000) Alzheimer’s amyloid fibrils: Structure and assembly Biochim Biophys Acta 1502, 16–30 Dobson CM (2001) Protein folding and its ... Gazit A recent study carried out by Kapurniotu and coworkers [19] paved the way towards the present understanding of the mechanism of amyloid self -assembly The authors first discovered that a hexapeptide...
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Báo cáo khoa học: A natural osmolyte trimethylamine N-oxide promotes assembly and bundling of the bacterial cell division protein, FtsZ and counteracts the denaturing effects of urea docx
... TMAO, monosodium glutamate can induce and enhance the assembly and bundling of FtsZ protofilaments suggesting that different osmolytes can affect FtsZ assembly and bundling of protofilaments differently ... measurements Urea and GdnHCl were dissolved in 25 mm Pipes, pH 6.8 for FtsZ assembly reactions Final pH of the urea and GdnHCl solutions was adjusted using HCl and NaOH Fresh solutions of urea and GdnHCl ... Where, SN, SI and SU represent the intrinsic signal intensities of the native, intermediate and the unfolded states, respectively DGNfiI and DGIfiU are the standard free energies for the NfiI and IfiU...
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oak composition and maturation influence
... ellagic and sometimes gallic acid A resulting compounds are both astringent and bitter and they are clearly unattractive to potential predators It is a major part of the process of seasoning and ... trans and influences all beverages which are matured in new - and sometimes used - American oak These two compounds come from small amounts of lipids - oils, fats and waxes - in the oak and increase ... it can play a role in bacterial action in wine maturation, but not whiskey maturation This view suggests that pairs of glucose units can break away and the resulting compound, cellobiose, can act...
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báo cáo hóa học:" Multiple functions of the von Willebrand Factor A domain in matrilins: secretion, assembly, and proteolysis" ppt
... Surgery and Research 2008, 3:21 members, matrilin-1 and matrilin-3 are expressed specifically in cartilage Matrlin-1 forms a homotrimer and matrilin-3 forms a mixture of homotetramer, -trimer, and ... domain of MATN3 To understand the structure-function relationship of cartilage-specific matrilins: MATN1 and 3, a series of cDNAs containing mutations and deletions in MATN1 and were constructed (Fig ... cloning of cDNA constructs, QC conceived the study, and participated in its design and coordination and drafted the manuscript All authors read and approved the final manuscript 15 16 17 Deak F,...
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Assembly and PCB Layout Guidelines for QFN Packages Introduction potx
... Solder Land Pitch (BL) 0.500 0.500 0.500 Line Width Between Via and Via Land (LW2) 0.100 0.100 0.100 Line Width Between Via outside and Via Land (LW1) 0.127 0.127 0.127 Line to Via Land (LV) ... underneath the package and its proximity to the inner edges of the lands Special considerations are needed in stencil design and paste printing for both perimeter lands and thermal pads Since ... Perimeter Lands and Thermal Pads The optimum and reliable solder joints on the perimeter pads should have about 50 to 70 µm (2 to mils) standoff height Stencils should be laser cut and electropolished...
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báo cáo khoa học: " Targeted isolation, sequence assembly and characterization of two white spruce (Picea glauca) BAC clones for terpenoid synthase and cytochrome P450 genes involved in conifer defence reveal insights into a conifer genome" pdf
... 3CAR (A) and CYP720B4(C) and comparison of 3CAR with the grand fir (Abies grandis) Gene structure of (LIM) and pinene synthase (PIN) genes (B) Gene structure of white spruce 3CAR (A) and CYP720B4 ... constitutive, and wound- and insect-induced expression, and cellular and subcellular localization in spruce and Douglas fir Plant Physiol 2007, 143:410-424 Liu JJ, Ekramoddoullah AK: Identification and ... (1,452 bp) and eight introns and includes transcribed 5'- and 3'-UTRs of 38 bp and 134 bp, respectively (Figure 2B) The intronic region covers 50% of the gene sequence between the start and stop...
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Báo cáo y học: " Design, assembly, and validation of a nose-only inhalation exposure system for studies of aerosolized viable influenza H5N1 virus in ferrets" ppt
... -and "dilution" air used to create the desired aerosol concentration and flow Air supplied to the system is controlled at precise flow rates and pressures with electronic and manual valves and ... in the air-handling and system components after assembly of the NBIES Leaks detected (if any) were sealed and the system repeatedly retested to verify remediation To improve safety and extend ... interpretation, and co-wrote the manuscript; WAS assisted with the assembly, testing, and validation of the system, and oversaw animal work; DED assisted with program management, invitro virus work, and...
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Báo cáo y học: "Highly conserved serine residue 40 in HIV-1 p6 regulates capsid processing and virus core assembl" potx
... affects CA maturation, virus core formation, and thus, infectivity Page 13 of 16 Methods Peptide synthesis and purification The synthesis, purification and molecular characterization of p6 and the ... through a 20% sucrose cushion and standardized for p24 content by ELISA Equal amounts of p24 were loaded on SDS-PAGE and analyzed by Western blotting The amount of MA and NC proteins in S40F mutant ... replication analyses in HLAC, X4 tropic (C and E) and R5 tropic (D and F), ng of p24 of purified virus carrying indicated mutations was used for infection and release of viral particles was determined...
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Báo cáo y học: " Human Polycomb group EED protein negatively affects HIV-1 assembly and release" pot
... (M) and insoluble pellet (P) (Fig 8a), and each fraction probed for Gag, EED and Nef proteins As expected, Gag polyprotein precursor and maturation products were mainly detected in fractions M and ... whereby EED and Nef were depleted from the membranes and redirected to a still undefined compartment EED did not inhibit Gag protein synthesis, and our results suggested that virus assembly and genome ... effect of EED on genome packaging and virus assembly could also be mediated by interactions of EED with genome ends [13], and viral proteins MA and/ or IN [11,12] WTNef and NefG2A, but not the NefΔ57...
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