cam dr hanging on for cell survival
Báo cáo khoa học: "Radioprotective effects of fucoidan on bone marrow cells: improvement of the cell survival and immunoreactivity" doc
... University (Korea) Irradiation on cells was performed once at Gy for this study Determination of interleukin-12 (IL-12) and tumor necrosis factor-alpha (TNF-alpha) production Fucoidan (50 μg/ml) was ... fucoidan on BMC viability, we cultured BMCs at a concentration of × 10 cells/well in 96-well plates and treated them with fucoidan before irradiation (Fig 1) After culturing the cells, CCK-8 solution ... from cytokine withdrawalinduced cell death and irradiation Fucoidan treatment decreases cell death in irradiated BMCs For this test, BMCs were cultured at a concentration of × 10 cells/ml in 6-well...
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báo cáo khoa học: "Effects of plasma concentrations of 5-fluorouracil on long-term survival after treatment with a definitive 5-fluorouracil/cisplatin-based chemoradiotherapy in Japanese patients with esophageal squamous cell carcinoma" doc
... erosion, a granular protruded lesion, an ulcer scar, and 1.2 w/v% iodine/glycerin-voiding lesions did not prevent an evaluation of CR The evaluations were performed every month for the first months, ... Figure A course consisted of the continuous infusion of 5-FU at 400 mg/m2/ day for days 1-5 and 8-12, the infusion of CDDP at 40 mg/m2/day on days and 8, and the radiation at Gy/ day on days to 5, ... report on the effects of plasma concentrations of 5-FU on long-term survival in cases of esophageal cancer Page of Methods Patients Forty-nine ESCC patients were enrolled in this study based on the...
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báo cáo khoa học: " A simple and high-sensitivity method for analysis of ubiquitination and polyubiquitination based on wheat cell-free protein synthesis" potx
... required for E2 activity [8,9] In addition, ubiquitination of substrates without polymerization, mono-ubiquitination, acts as a sorting signal for protein endocytosis and as a regulation factor for ... system and used for several functional analyses For screening, conventional detection methods such as immunoblot analysis or radioisotope-labeled proteins are not suitable for the detection of a large ... Construction of DNA Templates for Transcription We used RAFL as templates DNA templates of E2s and E3s for transcription were constructed by "Split-Primer" Page of 11 (page number not for citation purposes)...
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Báo cáo khoa học: "Pulse high-volume haemofiltration for treatment of severe sepsis: effects on hemodynamics and survival" doc
... reflected in one study [10] by failure to demonstrate an improvement in organ dysfunction and survival Hence, the indication for its use in septic patients was abandoned, beyond its function to provide ... vasopressors (noradrenaline, adrenaline and dopamine) were discontinued on day Available online http://ccforum.com/content/9/4/R294 Table Effects of pulse high-volume haemofiltration on haemodynamic ... to the patient's condition and clinical need Measurements Haemodynamic monitoring was done using a thermodilution pulmonary artery catheter with continuous cardiac output monitoring (Vigilance;...
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Allottware RA add on for WinCC v1 1
... template from the drop-down list Rev: 2, Feb 2005 Page 15 of 37 RA Add -On for WinCC User Manual 3.3 RA Add -On for WinCC v1.1 Navigation Configuration 3.3.1 Overview In the navigation tree you build ... adding containers and subcontainers All operations are performed from the right-click context menu All your pictures are displayed at the bottom Drag and drop the pictures onto the containers ... button has a caption and an authorization The caption is simply the text to be displayed on the menu button The authorization is the standard WinCC user authorization that is required to click on...
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Outdoor Life - Disaster Survival Guide - For Disaster Survival Skills
... sure sign the electrons have gone on strike You never know how long a power outage is going to last, so it’s wise to break out the pack of cards and prepare yourself for a long one just in case ... and death In an attack, you’ll likely only have seconds to reach your mask and put it on, so practice until it’s second nature STEP ONE With your thumbs on the inside of the mask, hold it by ... the location of the lifeboat-muster area for your cabin Then go find it If there’s an evacuation drill, attend and pay attention In an emergency, the captain will sound an alarm, consisting of...
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Tài liệu Báo cáo khoa học: Role of ceramide kinase in peroxisome proliferatoractivated receptor beta-induced cell survival of mouse keratinocytes ppt
... keratinocyte survival K Tsuji et al NIH 3T3 fibroblasts and A549 lung cancer cells, treatment with exogenous C1P at low concentrations enhanced cell survival, whereas high concentrations of C1P reduced cell ... Activation of PPARb enhances cell survival and inhibits cell death induced by serum starvation stress (A) SP1 cells under serum starvation stress treated with LD SP1 cells were cultured for an ... fetal bovine serum (control conditions) [Stress (+) or Stress ()), respectively] for 24 h SP1 cells were observed under a phase-contrast microscope (B) Effect of LD on the cell survival rate of mouse...
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Tài liệu Báo cáo khoa học: It is all about resolution Meeting report based upon presentations at the 10th International Global BioMillennium 2006 symposium on molecular cell biology (Tbilisi, Georgia) docx
... two-photon microscopy for studying how neuronal dendrites form in adult-born neurons that continuously develop into the olfactory bulb Lentivirus green fluorescent protein-labeled neurons were ... follow dendritic development in vivo Within a single day, neuronal morphology changed dramatically, with both formation and retraction of whole dendritic trees After 10 days, most of the neurons were ... DRP-1, mediate trans-phosphorylation and subsequent functional activation of ZIPk DAP5 is a translation initiation factor that directs internal ribosome entry site (IRES)-dependent translation...
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Cytoskeleton reorganization mediates alpha beta integrin-associated actions of laminin on proliferation and survival, but not on steroidogenesis of ovine granulosa cells pdf
... actions on GC steroidogenesis, independently of LN actions on cell shape In contrast, GC survival and proliferation are probably closely associated with cell shape and cytoskeleton organization ... < 0.01 for cells cultured on a substratum http://www.rbej.com/content/3/1/19 containing at least 43% LN), a decrease in proliferation rate (p < 0.01 for cells cultured on a substratum containing ... stimulus [70] For example, ERK1/2 activation by gonadotropins inhibits Star protein expression and progesterone secretion, thereby contributing to desensitization mechanisms of hormonal action [71,72]...
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Báo cáo khoa học: Two functionally redundant isoforms of Drosophila melanogaster eukaryotic initiation factor 4B are involved in cap-dependent translation, cell survival, and proliferation doc
... that Dm-eIF4B is required for cell survival and that it stimulates cell proliferation Experimental procedures Construction of plasmids PCR amplification was performed on the EST LD09953 to introduce ... eIF4B is required for cell survival, additional eIF4B reduction by RNAi treatment may explain why the cells died In yeast, eIF4A is required for cell survival during starvation [62], suggesting ... isoforms have a redundant positive effect on cap-dependent mRNA translation but not intervene in IRES-dependent translation Fig Drosophila eIF4B is required for cell survival (A) Localization...
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Báo cáo khoa học: Molecular and functional characterization of a novel splice variant of ANKHD1 that lacks the KH domain and its role in cell survival and apoptosis docx
... transfection Forty-eight hours post transfection, the cells were lysed and the whole cell proteins were extracted One hundred micrograms of total protein were used for immunoprecipitation (IP) ... effect, NT2 cells were transfected with different concentrations of VBARP siRNA or control siRNA and assayed for functional effects First, to confirm that VBARP Fig Subcellular distribution of VBARP-L ... transfected with the various concentration of VBARP or control siRNA in triplicate Forty-eight hours post transfection, cells were assayed for cell viability Percentage of viable cells in mock transfected...
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Báo cáo khoa học: A mammalian monothiol glutaredoxin, Grx3, is critical for cell cycle progression during embryogenesis doc
... images of control (e) and Grx3-KD (f) staining cells Scale bars = 200 lm (F) Quantification of binucleated cells in control and Grx3-KD cells Total 600 cells counted for control cells and 360 cells ... have shown that disruption of iron regulation and ⁄ or intracellular iron accumulation, which subsequently causes a Fenton reaction, may account for the sensitivity of mutant cells to excess oxidants ... different among the tested conditions For example, MmGrx3 expression was not induced when cells were treated with H2O2 for 0.5 h, but was enhanced at h (Fig 3C) In contrast, when cells were treated...
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Office home and student 2010 all- in -on For Dummie pdf
... group buttons Group buttons Groups tell you what the buttons and galleries above their names are used for On the Home tab in Excel, for example, the buttons in the Font group are for formatting ... of Contents xxi Chapter 3: Formulas and Functions for Crunching Numbers 425 How Formulas Work 425 Referring to cells in formulas 425 Referring to formula results in formulas ... button is located on the right side of the Ribbon, to the left of the Help button You can also right-click the Ribbon and choose Minimize the Ribbon on the shortcut menu, or double-click a tab on...
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Báo cáo khoa học: Optimization of an Escherichia coli system for cell-free synthesis of selectively 15N-labelled proteins for rapid analysis by NMR spectroscopy pdf
... functional homologues with similar threedimensional structures and amino acid compositions, and the codon usage of the CYPA gene had been adjusted for the E coli expression system by construction ... transcription/translation system As many modern plasmid vectors used for protein overproduction are based on transcription from the T7 promoter, it is convenient if the same vectors can be used in the cell- free ... gene, using a combination of ligation and recursive overlap extension of complementary synthetic oligonucleotides as described above Oligonucleotides used for the construction of the s-CYPA are...
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AUTOPHAGY - A DOUBLE-EDGED SWORD CELL SURVIVAL OR DEATH? pptx
... extended conformation and formed a lattice contact with the hydrophobic surface patches of a neighbouring molecule [37] The authors speculated that this interaction might allow for the formation of ... splitting for backbone amide groups in several segments, which is indicative of conformational exchange on an intermediate to slow (millisecond to second) time scale [48] These regions, which ... the crystallization conditions, favouring a peculiar lattice contact, our data confirm that the N-terminal portion of GABARAP exhibits an equilibrium of two or more conforma‐ tions In fact, preliminary...
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Anoxia Evidence for Eukaryote Survival and Paleontological Strategies pdf
... INTRODUCTION TO ANOXIA conditions In some anoxic single eukaryotic cells, the mitochondria are replaced by hydrogenosomes, or the mitochondrion is adapted as an unusual organelle structure for the ... oxygen depletion down to anoxia rather than a specific level of dissolved oxygen Sedimentologists often condense conflicting redox conditions to the reconstruction of either “euxinic” conditions, which ... Most investigations center on ecology, biomass, food webs, and stable isotope fractionation Reasonable for getting involved in this book was the rejection of an early manuscript on foraminifera...
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Báo cáo hóa học: " Non-monotonic changes in clonogenic cell survival induced by disulphonated aluminum phthalocyanine photodynamic treatment in a human glioma cell line" pot
... (about cell cycles), cell survival using colony-forming assay was measured after 10-12 days (time required for formation of visible macro-colonies containing at least 50 cells viz after completion ... increasing concentration of the sensitizer The subcellular distribution of AlPcS2 also varied with its extracellular concentration While cell proliferation kinetics showed a monotonic increase ... Beckton and Dickenson, USA) and data acquired using the Cell Quest software (Beckton and Dickenson, USA) Cell cycle analysis was performed using the Modfit program 2.6.3 Micronuclei formation Air-dried...
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Báo cáo hóa học: " Effects of pegylated G-CSF on immune cell number and function in patients with gynecological malignancies" doc
... the stimulation of IL-10 and IFN-a production [11] On a molecular level, G-CSF may determine mitochondrial dysfunction and proliferation arrest in T cells [12] G-CSF-mobilized monocytes acquire ... comparisons were performed with the MannWhitney test for paired or unpaired data, or with the Kruskal-Wallis test with Dunn’s correction for multiple comparisons, as appropriate The criterion for ... distribution of CD4 + T cells, CD8 + T cells, CD19 + B cells and NK cells (defined as CD3 - CD16 + CD56 + cells) within the lymphocyte population was unaffected by pegfilgrastim administration (data...
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Báo cáo sinh học: "Shipping blood to a central laboratory in multicenter clinical trials: effect of ambient temperature on specimen temperature, and effects of temperature on mononuclear cell yield, viability and immunologic function" potx
... E, Fenton T, for the Pediatric ACTG Cryopreservation Working Group: Optimization and limitations of use of cryopreserved peripheral blood mononuclear cells for functional and phenotypic T -cell ... division of the raw data by the donor value at RT for 24 h Since donors served as blocks and contributed an observation from each condition (i.e each combination of temperature and duration level), ... temperature conditions, compared to RT control conditions Blood from normal donors was incubated 24 h at RT (22°C, control), and for 2-12 h at 15, 22, 30 or 40°C (Exptl), then RT for the remainder...
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báo cáo hóa học:" Effect of borax on immune cell proliferation and sister chromatid exchange in human chromosomes Malinee Pongsavee" docx
... spectrophotometer The absorption maximum is dependent on the solvent employed This reduction takes place only when mitochondrial reductase enzymes are active, and therefore conversion can be directly related ... due to high consumption it may be accumulative Forty lymphocyte cultures from forty blood samples were tested in each borax concentration After 24 hours incubation, various concentrations of borax ... duplicate fashion Mean absorbance was calculate for the control wells and for each borax concentration in the test wells The degree of immune cell proliferation (lymphocyte proliferation) sensitivity...
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