calcium calmodulin kinase modulation activity
Báo cáo Y học: Autophosphorylation-dependent inactivation of plant chimeric calcium/calmodulin-dependent protein kinase docx
... lost 28% activity at pH 7.5, 54% activity at pH 6.5 and 72% activity at pH 8.5 in 30 s after autophosphorylation Kinase assays The autophosphorylation-induced changes in the kinase activity was ... site of plant chimeric calcium/ calmodulin- dependent protein kinase J Biol Chem 276, 32940–32947 13 Colbran, R.J & Soderling, T.R (1990) Calcium/ calmodulindependent protein kinase II Curr Top Cell ... chimeric plant serine/threonine kinase by calcium and calcium/ calmodulin J Biol Chem 271, 8126–8132 12 Sathyanarayanan, P.V., Siems, W.F., Jones, J.P & Poovaiah, B.W (2001) Calcium- stimulated autophosphorylation...
- 7
- 251
- 0
Báo cáo Y học: Transient activation of the c-Jun N-terminal kinase (JNK) activity by ligation of the tetraspan CD53 antigen in different cell types pptx
... ligation (Fig 2A) However, Jun phosphorylation could also be mediated by the p38 kinase when using endogenous kinase activity To overcome this possibility and to confirm the role of JNK, we transfected ... whole cell extracts with an anti-HA antibody to separate it from the endogenous kinase After separation, the kinase activity was determined in the immunoprecipitate using two different substrates, ... endogenous JNK activity, the cells were incubated for h in the presence of MEM53 antibody The luciferase activity was corrected for the efficiency of transfection by determining the activity of plasmid...
- 10
- 517
- 0
Báo cáo khoa học: Protein kinase Ch activity is involved in the 2,3,7,8tetrachlorodibenzo-p-dioxin-induced signal transduction pathway leading to apoptosis in L-MAT, a human lymphoblastic T-cell line potx
... Journal 272 (2005) 903–915 ª 2005 FEBS To establish whether TCDD increases nPKC kinase activity, we examined nPKC kinase activity by using PKCh pseudo-substrate, which is preferentially phosphorylated ... involvement of PKCh kinase activity in TCDD-induced L-MAT cell apoptosis at the molecular level Transfected L-MAT cells express H-2KK on their surface To test whether PKCh kinase activity is required ... interfere with endogenously expressed PKCh and specifically sup906 press its kinase activity To confirm that PKCh kinase activity really does participate in the signal transduction mechanism involved...
- 13
- 426
- 0
Tài liệu Báo cáo khoa học: Modulation of F0F1-ATP synthase activity by cyclophilin D regulates matrix adenine nucleotide levels pptx
... involved in the modulation of PTP open closed probability CYPD is a member of the cyclophilins family encoded by the ppif gene [3], which exhibit peptidylprolyl cis trans isomerase activity Inhibition ... diminish the binding of CYPD on the ATP synthase The results obtained indicate that modulation of F0F1-ATP synthase activity by CYPD comprises an in-house mechanism regulating matrix adenine nucleotide ... and F0F1-ATP synthase activity depends on the common terms Ti and Di We were therefore able to calculate the changes in To and Do, assuming an increase in F0F1-ATP synthase activity of 30%, (as...
- 14
- 627
- 0
Tài liệu Báo cáo khoa học: Dual P2Y12 receptor signaling in thrombin-stimulated platelets – involvement of phosphoinositide 3-kinase b but not c isoform in Ca2+ mobilization and procoagulant activity pdf
... measurement of SERCA activity in saponin-permeabilized platelets showed that neither AR-C nor wortmannin decreased this activity by < 3% Together, these results indicate that ADP ⁄ P2Y12 activity prolongs ... ineffective Further experiments confirmed the sensitivity of InsP3-induced Ca2+ mobilization for modulation of PKA activity Platelets were therefore pretreated with the PKA inhibitors, KT5720 and H89 [28] ... effect of cAMP and its effector, protein kinase A (PKA) [14] Also downstream of Gi, P2Y12 receptors stimulate the less well understood phosphoinositide 3 -kinase (PI3-K) pathway, which leads to...
- 15
- 565
- 0
Báo cáo khoa học: Ca2+/calmodulin-dependent kinase II signalling cascade mediates P2X7 receptor-dependent inhibition of neuritogenesis in neuroblastoma cells docx
... calmodulin- dependent kinase II (CaMKII) activity and some of its downstream effectors, including focal adhesion kinase (FAK), phosphatidylinositol 3 -kinase (PI3K), Akt and glycogen synthase kinase ... receptors induce calcium ⁄ calmodulin- dependent protein kinase II phosphorylation in cerebellar granule neurons Eur J Neurosci 23, 2999–3013 56 Robles E & Gomez TM (2006) Focal adhesion kinase signaling ... receptor activation rapidly stimulates focal adhesion kinase phosphorylation at Ser-843 Mediation by Ca2+, calmodulin, and Ca2+ ⁄ calmodulin- dependent kinase II J Biol Chem 280, 24212–24220 58 Chen...
- 19
- 328
- 0
Báo cáo khoa học: Acidic extracellular pH increases calcium influx-triggered phospholipase D activity along with acidic sphingomyelinase activation to induce matrix metalloproteinase-9 expression in mouse metastatic melanoma pot
... phosphorylation of extracellular signal regulated kinase ⁄ and p38, but they suppressed nuclear factor-jB activity These data suggest that the calcium influx-triggered phospholipase D and acidic ... on PLD activity, cells were cultured with the [Ca2+]i chelater BAPTA-AM We found that this reagent dose-dependently reduced acidic pHe-induced PLD activity, but had no effect on aSMase activity ... increased aSMase activity 2.0-fold but had no effect on nSMase activity (Fig 3B) When aSMase blockers were added to the cultures, they significantly inhibited acidic pHe-induced aSMase activity (Fig...
- 13
- 409
- 0
Báo cáo khóa học: SUT2 is a novel multicopy suppressor of low activity of the cAMP/protein kinase A pathway in yeast docx
... wild type requires high PKA activity These consequences of PKA-pathway activity were tested subsequently in order to examine the influence of high copy SUT2 on PKA activity We found that high ... in PKA pathway activity On this basis, we hypothesized that (a) high SUT2 gene dosage might increase PKA activity only when it is low, but that (b) it acts inhibitory when PKA activity is high ... that a high level of SUT2 reduces PKA activity only when PKA activity is high we investigated the influence of SUT2 on invasive growth, which requires high PKA activity We found that after three...
- 8
- 485
- 0
Báo cáo khoa học: Cytotoxic activity of nucleoside diphosphate kinase secreted from Mycobacterium tuberculosis pptx
... hydrolysis activity (Fig 3) Thus, H117 is crucial for ATPase activity Secretion of nucleoside diphosphate kinase by M tuberculosis M tuberculosis H37Rv culture supernatant exhibited Ndk activity ... adenylate kinase (a cytoplasmic protein) was not detected by Western blot using polyclonal antibody against purified adenylate kinase (Fig 5B) Autophosphorylation activity The autophosphorylating activity ... separated by 15% SDS/PAGE Analysis was by autoradiography Enzymatic activity of Ndk Enzymatic activity of purified Ndk or its activity in culture supernatant of M tuberculosis was assayed as described...
- 10
- 342
- 0
Báo cáo khoa học: Molecular cloning of the Matrix Gla Protein gene from Xenopus laevis Functional analysis of the promoter identifies a calcium sensitive region required for basal activity doc
... standard calcium phosphate coprecipitation technique [22] To evaluate dose-dependent effects of extracellular calcium on MGP transcription cells were grown in medium supplemented with either calcium ... transfection Luciferase activity was assayed as recommended by the manufacturer (Promega) in a ML3000 luminometer (Dynatech) Relative light units were normalized to b-galactosidase activity and protein ... promoter activity (Fig 5) A reporter construct containing only the promoter region from position )54 to +33 bp, including the TATA box ()54LUC), showed a drastic drop in luciferase activity Internal...
- 10
- 475
- 0
Báo cáo khoa học: Inhibition of the NF-jB transcriptional activity by protein kinase A pot
... luciferase activity with pRL-TK plasmid containing TK promoter as an internal control All luciferase activities shown in transient transfection assays are corrected by the internal control activity ... cAMP and subsequent activation of PKA lead to the inhibition of NF-jB activity To further examine the effect of PKA on NF-jB activity, we cotransfected Jurkat T-lymphocytes and HeLa cells with ... CRE-dependent luciferase activity in Jurkat T-lymphocytes As expected, overexpression of PKAc led to an increase in the CRE-dependent gene expression (Fig 2C) binding activity of NF-jB, we performed...
- 7
- 296
- 0
Báo cáo khoa học: Role of Ca2+/calmodulin regulated signaling pathways in chemoattractant induced neutrophil effector functions Comparison with the role of phosphotidylinositol-3 kinase ppt
... protein calmodulin [20] Calmodulin is activated by binding of four Ca2+ ions, resulting in conformational change Once calmodulin is activated it can bind to downstream targets, such as the Ca2+ /calmodulin- dependent ... found to be dependent on calmodulin activity, but not on PtdIns3K However, fMLP induced respiratory bursts demonstrated a dependence on both calmodulin and PtdIns3K activity These data demonstrate ... inhibition of neutrophil migration by W7 (Fig 4) A calmodulin- dependent kinase that might be involved in this process is myosin light chain kinase This kinase phosphorylates the light chain of myosin...
- 10
- 537
- 0
Báo cáo khoa học: Casein phosphopeptide promotion of calcium uptake in HT-29 cells ) relationship between biological activity and supramolecular structure ppt
... enhancement of calcium absorption in growing rats Int J Vit Nutr Res 68, 335–340 23 Tsuchita H, Suzuki T & Kuwata T (2001) The effect of casein phosphopeptides on calcium absorption from calcium- fortified ... phosphopeptide on calcium absorption in the rat Br J Nutr 83, 673–680 45 Mellander O (1950) The physiological importance of the casein phosphopeptide calcium salts II Peroral calcium dosage of ... ª 2007 FEBS 5001 CPP activity and supramolecular structure C Gravaghi et al KRH (containing phosphate) phosphate-free KRH A B 100 [Ca2+]o 2mmol/L 100 [Ca2+]o 4mmol/L peak calcium increase (% on...
- 13
- 468
- 0
Báo cáo khoa học: Coiled–coil interactions modulate multimerization, mitochondrial binding and kinase activity of myotonic dystrophy protein kinase splice isoforms pptx
... heteromultimerization occurs independently of kinase activity and alternatively spliced domains To confirm that DMPK self-association indeed occurred independently of kinase activity, a kinase dead mutant was tested ... Multimerization of myotonic dystrophy protein kinase (DMPK) is independent of kinase activity or alternatively spliced domains (A) Involvement of kinase activity in DMPK complex formation was investigated ... between these possibilities In DMPKlike kinases, multimerization capacity is apparently a prerequisite for proper kinase activation: the kinase activity of Rho -kinase and MRCKa is also partly dependent...
- 13
- 392
- 0
Báo cáo khóa học: Phospholipase C, protein kinase C, Ca 2+ /calmodulin-dependent protein kinase II, and redox state are involved in epigallocatechin gallate-induced phospholipase D activation in human astroglioma cells ppt
... [Ins(1,4,5)P3–Ca2+]–Ca2+ /calmodulin- dependent protein kinase II (CaM kinase II)–PLD pathway and the PLC-c1 (diacylglycerol)–PKC–PLD pathway Experimental procedures PLD assay PLD activity was assessed by measuring ... EGCG-induced PLD activity in these cells We also investigated whether EGCG induces PLC activity in U87 cells The data presented in Fig 3C show that EGCG treatment stimulates PLC activity, as measured ... cytosolic calcium Furthermore, the results suggest that an influx of calcium from the extracellular medium is mainly responsible for this rise Fig Green tea polyphenols stimulate PLD activity in...
- 11
- 279
- 0
Báo cáo Y học: Irregular spiking in free calcium concentration in single, human platelets Regulation by modulation of the inositol trisphosphate receptors ppt
... 1,4,5-trisphosphate-induced calcium release Science 252, 443–446 Bezprozvanny, I., Watras, J & Ehrlich, B.E (1991) Bell-shaped calcium- response curves of Ins(1,4,5)P3- and calcium- gated channels from ... the presence of increased calcium Nature 396, 81–84 Missiaen, L., Taylor, C.W & Berridge, M.J (1991) Spontaneous calcium release from inositol trisphosphate-sensitive calcium stores Nature 352, ... 1.25 mM NaN3 (prepared in calciumfree water) The mixture, in a fluorescence cuvette, was supplemented with 7.5 mM phosphocreatine, mM ATP, mM KH2PO4, 30 lgÆmL)1 creatine kinase, 0.6 lgÆmL)1 oligomycin...
- 10
- 533
- 0
Báo cáo khoa học: The starch-binding capacity of the noncatalytic SBD2 region and the interaction between the N- and C-terminal domains are involved in the modulation of the activity of starch synthase III fromArabidopsis thaliana pdf
... SSIII activity modulation by SBD cannot be deduced until the enzyme conformation is elucidated, the data presented here contribute to a better understanding of how SBDs modulate enzyme activity, ... catalytic activity of the enzyme Our results show that the interaction between SBDs and CD, as well as the full starch-binding capacity of the D2 domain, are necessary for the full catalytic activity ... truncated SSIII isoforms, showing the importance of the D23 domain in starch binding and the modulation of SSIII activity [19] A similar conclusion has been reached for other enzymes involved in starch...
- 13
- 457
- 0
Báo cáo khoa học: Modulation of the Arabidopsis KAT1 channel by an activator of protein kinase C in Xenopus laevis oocytes potx
... Ca2+-independent ABA-activated SnRK2.6 kinase [21] In addition, the SnRK2.6 kinase could phosphorylate T306 in an in vitro kinase assay This evidence suggests that multiple protein kinases may participate in ... phosphorylation by calcium- dependent protein kinase from maize seedlings Eur J Biochem 267, 337–343 Li J, Lee YR & Assmann SM (1998) Guard cells possess a calcium- dependent protein kinase that phosphorylates ... control of guard cell volume The calcium- independent, ABA-activated protein kinase ⁄ ABA-responsive kinase (AAPK ⁄ ABR kinase) from Vicia faba has been found to be present in guard cells and to...
- 11
- 531
- 0
Báo cáo khoa học: Suppression of nuclear factor-jB activity in macrophages by chylomicron remnants: modulation by the fatty acid composition of the particles pot
... control Luciferase activity (light units) A 40 000 30 000 Untransfected Control + CRLPs 20 000 ** 10 000 B Luciferase activity (light units) sites can be used as a measure of NF-jB activity Initial ... (Fig 1) Inhibition of NF-jB transcriptional activity by CRLPs containing trilinolein or triacylglycerol from corn oil was confirmed by measuring luciferase activity in cells transfected with the pNF-jB ... olive CRLPs (Fig 6) NF-jB activity in control samples did not vary significantly over the time-course examined In the canonical NF-jB pathway, activation of the IjB kinase complex leads to phosphorylation...
- 14
- 328
- 0
Báo cáo khoa học: A systems biological approach suggests that transcriptional feedback regulation by dual-specificity phosphatase 6 shapes extracellular signal-related kinase activity in RAS-transformed fibroblasts pptx
... ERK activity for intermediate stimuli The distribution of ERK activity is shown in Fig 4B In contrast to bistable responses, the stimulation experiments showed a monomodal distribution of ERK activity, ... gradually shifts to higher activity levels as the stimulus increases This suggests that ERK activity is not bistable in fibroblasts stimulated with PDGF The average activity shows a Hill-type ... protein kinase (MAPK) pathway model Biotechnol Prog 17, 227–239 10 Kholodenko BN (2000) Negative feedback and ultrasensitivity can bring about oscillations in the mitogen-activated protein kinase...
- 12
- 169
- 0
Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose