ca2 handling proteins in hesc cms
Tài liệu Báo cáo khoa học: Subproteomics analysis of Ca2+-binding proteins demonstrates decreased calsequestrin expression in dystrophic mouse skeletal muscle pdf
... approach using antibodies and the ÔStains-AllÕ dye, The cationic carbocyanine dye ÔStains-AllÕ was used to determine potential changes in the expression of major Ca2+ -binding proteins in dystrophic ... preventing muscle fibre degeneration Thus, drug-based alterations in Ca2+ cycling may be useful in avoiding Ó FEBS 2004 Ca2+ -binding proteins and muscular dystrophy (Eur J Biochem 271) 3951 Ca2+ -related ... MacLennan, D.H & Jorgensen, A.O (1983) Staining of the Ca2+ -binding proteins, calsequestrin, calmodulin, troponin C, and S-100, with the cationic carbocyanine dye ÔStainsallÕ J Biol Chem 258, 11267–11273...
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Tài liệu Business values, management and conflict handling: issues in contemporary Singapore ppt
... Competing Collaborating Compromising Avoiding Accommodating Production and engineering Competing Collaborating Compromising Avoiding Accommodating Avoiding Accommodating Avoiding Accommodating Information ... Competing Collaborating Compromising Avoiding Accommodating Avoiding Accommodating Avoiding Accommodating Avoiding Accommodating Students Competing Collaborating Compromising Armed forces Competing ... (male) Key : Chinese (female) Competing Collaborating Indian (male) Indian (female) Compromising Avoiding Malay (male) Accommodating Malay (female) Figure Modes of handling conflict in relation...
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Tài liệu Báo cáo khoa học: Mitochondrial targeting of intact CYP2B1 and CYP2E1 and N-terminal truncated CYP1A1 proteins in Saccharomyces cerevisiae ) role of protein kinase A in the mitochondrial targeting of CYP2E1 pdf
... 1A1 proteins were localized inside of the inner membrane Moreover, the imported + ⁄ 1A1 and + 33 ⁄ 1A1 proteins were extractable with alkaline Na2CO3 as shown in Fig 2C, suggesting that both proteins ... suggesting that an open N-terminal signal domain is critical for protein targeting to the ER (Fig 2D) In contrast to the targeting of CYP1A1 requiring N-terminal truncation, we have shown that intact ... mitochondria were incubated with increasing concentrations of digitonin (0–0.4%; 0–400 lgÆmg)1 protein) for 30 as described in the text The digitonin-insoluble (pellet, left panel) and digitonin-soluble...
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Tài liệu Báo cáo khoa học: Bone morphogenetic proteins in the early development of zebrafish pptx
... family, binding to and inhibiting these signaling molecules from binding to their receptors in the extracellular space, thus inhibiting ventralizing activities Of these proteins, Chordin has a ... regarding the function of tsg in the BMP pathway probably will remain unresolved for some time Other factors affecting the BMP pathway Factors inhibiting BMP2b, other than direct binding proteins ... mfn (C3), indicating that Tld (Tll1) also functions to pattern dorsoanterior structures [29] Twisted gastrulation (Tsg), a BMP-binding protein, initially found in Drosophila, is involved in dorsal–...
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Tài liệu Báo cáo khoa học: DNA modification with cisplatin affects sequence-specific DNA binding of p53 and p73 proteins in a target site-dependent manner pptx
... sequence-specific binding of p53 or p73 proteins to a 50-mer oligonucleotide (oligo) involving the p53 DNA-binding sites (p53DBSs) (A) The 32P-labeled p21 target was incubated with the given protein in presence ... protein was decreased due to formation of cisplatin adducts within the p53 DNA-binding site (p53DBS), resulting in increased p53 binding to the labeled probe The pPGM4 fragment (c) contains cisplatinresistant ... complexes [10] In addition, interactions of the p53 protein with certain response elements can be controlled by changes in DNA topology inducing formation of non-B DNA structures within the binding sites...
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Tài liệu Báo cáo khoa học: Integral membrane proteins in the mitochondrial outer membrane of Saccharomyces cerevisiae docx
... containing peripheral membrane proteins, a detergent-phase in which proteins with a-helical transmembrane domains are soluble and a ‘phospholipid-enriched’ pellet fraction containing proteins ... inner membrane However, since we identified no other inner membrane proteins in our vesicle preparation, we suggest that Gut2 is an outer membrane protein with its N-terminal FAD-binding domain ... size of proteins enriched in the lipid-rich pellet including the major 29 kDa protein Por1 and Tom40, identified by mass spectrometry and immunoblotting (C) After in situ digestion with trypsin, mass...
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Tài liệu Báo cáo khoa học: Enhanced expression of Mcm proteins in cancer cells derived from uterine cervix docx
... cellular proteins but also in chromatin-bound proteins The Mcm proteins bound to chromatin probably function in DNA replication, but the role of the proteins not tightly bound to chromatin remains to ... comparing the amounts of Mcm in total cellular proteins and chromatin-bound proteins, approximately one-third of total Mcm protein was found to be recovered in the chromatin-bound fraction in HeLa ... protein After the band intensities were quantitated in each protein, the concentrations of these proteins in total and chromatin-bound fraction were determined The relative ratio (HeLa/WI) in the...
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Tài liệu Báo cáo Y học: Systematic search for zinc-binding proteins in Escherichia coli potx
... Dose-dependent binding of Zn(II) to purified E coli zinc-binding proteins The newly identified E coli Zn(II)-binding proteins, AckA and Ppa, were analysed, together with the known Zn(II)-binding proteins, ... coli genome) Most of the Zn(II)binding proteins herein detected not contain any of the known zinc-binding motifs, which were identified in zinc-containing proteins from higher eukaryotes After ... systematic search for E coli proteins Ó FEBS 2002 Escherichia coli zinc-binding proteins (Eur J Biochem 269) 2411 Table Zinc-binding proteins in Escherichia coli Protein Function Reference Ada Cdd...
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Báo cáo khoa học: Nuclear actin and actin-binding proteins in the regulation of transcription and gene expression docx
... Direct a-actinin-2 ()) Bundling proteins Coactivator Supervillin NLS F-actin- and membraneassociated scaffolding protein Filamin NLS? Cross-linking proteins Filamin A NLS? Cross-linking proteins Transgelin ... nuclear actin-binding proteins interacting with the androgen receptor AR, androgen receptor; LBD, ligand-binding domain Actin-binding protein Targeting sequence Gelsolin Direct or indirect association ... members of the actin-binding LIM protein (ABLIM) family, ABLIM-2 and -3, as STARS-interacting proteins These novel proteins contain four LIM domains and a C-terminal villin headpiece domain, which mediates...
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Báo cáo khoa học: Structural and functional evidence for a singular repertoire of BMP receptor signal transducing proteins in the lophotrochozoan Crassostrea gigas suggests a shared ancestral BMP/activin pathway docx
... C2 domains were not directly adjacent but were joined by a linker sequence The intracellular kinase domain conforms to the canonical sequence of serine ⁄ threonine protein kinase domains seen ... Cg-BMPR1 and Cg-TGFbsfR2 is shown in Fig 2C The serine ⁄ threonine kinase domain in both proteins is encoded by two exons equivalent to kinase subdomains X and XI [22] In addition, the GS box and L45 ... in bold and underlined, and the cysteine knot is boxed Also boxed are the transmembrane domain, the ATP binding site, the L45 loop and the serine ⁄ threonine kinase domain (B) The implied amino...
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Báo cáo khoa học: Dynamin-related proteins and Pex11 proteins in peroxisome division and proliferation doc
... division in a more direct way Fig Domain structure of dynamins and dynamin-related proteins (DRPs) GED, GTPase effector domain; MD, middle domain; PH, pleckstrin homology; PRD, proline- and arginine-rich ... self-assembly, and a proline and arginine-rich domain (PRD) that mediates interactions with SH3 domains of effector proteins of the actin cytoskeleton Dynamins are required in phagocytosis and in caveolae- ... trafficking Dynamins are GTPases involved in intracellular fission processes [68–70] Five domains have been identified in conventional dynamins: a highly conserved N-terminal GTPase domain, a less...
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Báo cáo khoa học: LmbE proteins from Bacillus cereus are de-N-acetylases with broad substrate specificity and are highly similar to proteins in Bacillus anthracis pot
... lus circu lans OD is K s aen kar cus Fig Neighbor joining tree for 929 LmbE proteins Bacillus cereus proteins and proteins of known function are indicated c oco a kod rm 764 TK1 The FEBS Journal ... docking properties in the BC1534 active site, GlcNAc2 had the lowest calculated binding energy (data not shown), which is in agreement with the kinetic parameters shown in Table 2748 indicating ... Arrows indicate open reading frames Genes of interest are indicated by colored arrows pathway in which all of these genes could be involved In contrast, bc3461 is not part of a gene cluster, indicating...
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Báo cáo khoa học: Esculentin-1b(1–18) – a membrane-active antimicrobial peptide that synergizes with antibiotics and modifies the expression level of a limited number of proteins in Escherichia coli doc
... Ampicillin Carbenicillin Cephalosporin C Ceftazidime Chloramphenicol Erythromycin Imipenem Nalidixic acid Netilmicin Oxacillin Rifampicin Streptomycin Tazocin Tetracycline 64 128 0.5 32 0.5 8 ... lm SYTOX Green in NaCl ⁄ Pi for in the dark After addition of peptide, the increase in fluorescence, owing to the binding of the dye to intracellular DNA, was measured at 37 °C in a microplate ... with erythromycin, presumably by increasing the intracellular in ux of this drug, as a result of increased membrane permeability; (c) elicits identical changes in the bacterium’s protein expression...
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Báo cáo khoa học: Cellular response to unfolded proteins in the endoplasmic reticulum of plants pptx
... of ATP-binding HSP70-like proteins in the maize endosperm Plant Cell 3, 507–515 Zhang F & Boston RS (1992) Increases in binding protein (BiP) accompany changes in protein body morphology in three ... function of PDI proteins in plants has been reported The high-capacity calcium-binding proteins, calnexin (an ER transmembrane protein) [124,125] and calreticulin (an ER luminal protein) [126,127], ... role in maintaining the permeability barrier of the ER translocon during early stages of protein Response to unfolded proteins in ER of plants translocation [102], targeting misfolded proteins...
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Báo cáo khoa học: "Distributional Representations for Handling Sparsity in Supervised Sequence-Labeling" pptx
... appearing 0-2 times in the labeled training data, and appearing at least ten times in the HMM’s training data Table contains our results For our POS tagging experiments, we use 561 MEDLINE sentences ... Setup We investigate the use of smoothing in two test systems, conditional random field (CRF) models for POS tagging and chunking To incorporate smoothing into our models, we follow the following general ... Frustratingly easy domain adape tation In ACL Noah A Smith and Jason Eisner 2005 Contrastive estimation: Training log-linear models on unlabeled data In Proceedings of the 43rd Annual Meeting of...
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Báo cáo khoa học: Proteomic identification of all plastid-specific ribosomal proteins in higher plant chloroplast 30S ribosomal subunit PSRP-2 (U1A-type domains), PSRP-3a/b (ycf65 homologue) and PSRP-4 (Thx homologue) doc
... RNA-binding proteins (cpRNPs) present in the chloroplast stroma Other high-alignment hits were: polyadenylate-binding proteins, glycine-rich RNA-binding proteins, heterogeneous nuclear ribonucleoproteins ... translation of psbA mRNA is regulated by cis-elements including ribosome-binding sites and 5¢-UTR-binding proteins [16–18] Thus, while maintaining an overall resemblance to the eubacterial system, ... RNP, Chlamydomonas reinhardtii RB47 poly(A)-binding protein, barley cold-inducible glycine-rich RNA-binding protein, Anabaena variabilis RNA-binding protein, human hnRNP, and human U1A snRNP) shows...
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Báo cáo khoa học: Differential expression of liver and kidney proteins in a mouse model for primary hyperoxaluria type I pdf
... Phosphoglycerate mutase Indolethylamine N-methyltransferase Peroxiredoxin Apolipoprotein A-I Abhydrolase domain containing 14b D-Amino Protein name ApoA4 ActB Nsfl1C Acy1 Pgam1 Inmt Prdx6 ApoA1 Abhd14b ... serine:pyruvate ⁄ alanine:glyoxylate aminotransferase J Biol Chem 274, 16028–16033 11 Neuhoff V, Arold N, Taube D & Ehrhardt W (1988) Improved staining of proteins in polyacrylamide gels including ... indicate that its alanineglyoxylate aminotransferase activity is not favored over aminobutyrate-pyruvate, b-alanine-pyruvate and dimethylarginine-pyruvate aminotransferase activities In the rat, gluconeogenesis...
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Báo cáo khoa học: Proteomic characterization of lipid raft proteins in amyotrophic lateral sclerosis mouse spinal cord pot
... SDR family member Destrin (actin-depolymerizing factor) EF-hand domain containing protein 2, Swiprosin-1 Ezrin (p81) (cytovillin) (villin-2) Heat shock 27 kDa protein Integrin a-M precursor Lysosome-associated ... precursor Serine ⁄ threonine-protein phosphatase 2A catalytic subunit SH3 adapter protein SPIN90 (NCK-interacting protein with SH3 domain) Sushi domain-containing protein precursor FEBS Journal ... in G93A samples These proteins with differential abundances in WT and G93A lipid raft samples, as well as the alteration ratios, are listed in Table The remaining 113 proteins, including actin,...
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Báo cáo khoa học: Functional interplay between viral and cellular SR proteins in control of post-transcriptional gene regulation pptx
... SR proteins, together with other RNA-binding proteins, coordinate the coupling of splicing and polyadenylation Viral and cellular SR proteins enhancers of mRNAs [62] In addition, SR proteins ... kinases, including cyclin-dependent kinases, GSK, casein kinase II, mitogen-activated protein kinases, and SRPKs, may phosphorylate coronavirus N proteins [11,12] We have Several viral proteins ... HPV E2 protein interacts with cellular splicing factors, including prototypical SR proteins and RS domain-containing small nuclear ribonucleoprotein (snRNP) components [9] Functional investigation...
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Báo cáo khoa học: The consensus motif for N-myristoylation of plant proteins in a wheat germ cell-free translation system ppt
... )2.3 )0.9 0.3 Ca2+ -binding EF-hand family protein Plastid movement impaired (PMI2) Ca2+ -binding EF-hand family protein Protein kinase Zinc-finger (C3HC4-type RING finger) family protein Zinc-finger ... plant proteins, with N-myristoylation of several A thaliana proteins, such as a Ca2+ -dependent protein kinase, a GTPase, and RING finger-type ubiquitin ligases, having been demonstrated in vitro ... Ubiquitin lysine 63 chain forming ligases regulate apical dominance in Arabidopsis Plant Cell 19, 1898–1911 Podell S & Gribskov M (2004) Predicting N-terminal myristoylation sites in plant proteins...
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