c57bl 6 mice developed significant systemic ige and igg1 levels following allergenic sensitization and challenge with af comparable to the responses of balb c mice

Báo cáo y học: "Aspergillus antigen induces robust Th2 cytokine production, inflammation, airway hyperreactivity and fibrosis in the absence of MCP-1 or CCR2" pptx

Ngày tải lên : 12/08/2014, 18:21

... antigen induces AHR and mucus production even in the absence of MCP-1 or CCR2 Th2 cytokine and IgE production is similar in Aspergillus antigen-treated wild-type, Mcp-1-/- and Ccr2-/- mice To ... Aspergillus antigen in the absence of MCP-1 or its receptor, CCR2, we used mice with targeted disruptions of the genes that encode MCP-1 and CCR2 Since mouse strain differences are associated with major ... differences in antigen reactivity in many model systems, the mice used here were produced by extensive backcrossing into a C5 7BL/ 6 genetic background Both MCP-1- and CCR2-deficient mice developed...

12
290
0

Báo cáo khoa học: Theoretical study of lipid biosynthesis in wild-type Escherichia coli and in a protoplast-type L-form using elementary ﬂux mode analysis potx

Ngày tải lên : 22/03/2014, 21:20

... be of signiﬁcance if each of the forms of lipid A could be reimported from the outer membrane into the cytosol, as most of the lipid A is found in the former compartment According to the EcoCyc ... cycles of the fatty acid elongation more conveniently (Table 6) In the case of elongation of fatty acids, we decided to split the cycles into several parts and combined some of the reactions in these ... acids, metabolites correspond to fatty acids of different chain lengths: lauroyl-ACP corresponds to an acyl-ACP of length 12, myristoyl-ACP corresponds to an acyl-ACP of length 14, and palmitoleoyl-ACP...

12
553
0

Báo cáo y học: " Wild-type and central DNA flap defective HIV-1 lentiviral vector genomes: intracellular visualization at ultrastructural resolution levels" doc

Ngày tải lên : 13/08/2014, 09:20

... versus cytoplasmic vector DNA in the case of DNA Flap+ vector with an average nuclear/cytoplasmic ratio of 3.9 ± 0.9, which means that 77.3 ± 3 .6% of total vector DNA was detected within the nuclear ... nuclear compartment Conversely, there was a strong accumulation of cytoplasmic versus nuclear vector DNA in the case of DNA Flap- vector with an average nuclear/cytoplasmic ratio of 0.2 ± 0.04, which ... genome through the nuclear pore Other viral factors, and conceivably many cellular factors, are also expected to contribute to the active nuclear import of HIV-1 In the case of Flap+ vector genomes,...

7
204
0

Tài liệu CUSTOMS DECLARATION AND LIQUIDATION SOFTWARCUSTOMS DECLARATION AND LIQUIDATION SOFTWARE USAGE GUIDLINE FOR BUSINESS - PRODUCTION TYPE ECUS_KD 1.2 docx

Ngày tải lên : 13/12/2013, 00:15

... to operate the program, from transfer data to the Customs When you run the program again ,you must re-enter the account connecting to the Customs ( Contact the Custom’s IT staffs to receive connecting ... number of the declaration sheet incase there is no error 36 Print out the receiver number and bring it to the Customs Office to customs procedures with a click on button “in” on the window of inputing ... you can send the declaration sheet to the Customs Office Choose button “Khai báo”, the program software will send your declaration sheet to the Customs Office You will get the receiver number of...

58
609
0

Tài liệu Báo cáo khoa học: TMPRSS13, a type II transmembrane serine protease, is inhibited by hepatocyte growth factor activator inhibitor type 1 and activates pro-hepatocyte growth factor pdf

Ngày tải lên : 15/02/2014, 01:20

... GCAACA-3¢ and 5¢-ACAGGCAGCCTCGTCGGAGG-3¢ for HAI-1 ( 26 cycles) The GAPDH-speci c primer set, 5¢-AGGTGAAGGTCGGAGTCAAC-3¢ and 5¢-TACTCC TTGGGAGGCCATGTG-3¢, was used for control reactions (20 cycles) ... reaction product (1 lL) was ampliﬁed by PCR using the following gene-speci c primer sets: 5¢-TCCCATCTGTAGCAGCA ACT-3¢ and 5¢-GGATTTTCTGAATCGCACCT-3¢ for TMPRSS13 (34 cycles), and 5¢-ATGGAGGCTGCTTGG ... amino acid residues 315– 567 with the C- terminally tagged myc-His sequence was ampliﬁed by PCR, and subcloned into the EcoRI and PstI sites of an E coli expression vector, pMAL -c2 X To construct an...

13
641
0

Tài liệu Báo cáo khoa học: Golgi reassembly stacking protein 55 interacts with membrane-type (MT) 1-matrix metalloprotease (MMP) and furin and plays a role in the activation of the MT1-MMP zymogen pdf

Ngày tải lên : 18/02/2014, 04:20

... generated the VP 16- PC5/6B and VP 16- PC7 chimeras where the ICDs of PC5/6B and PC7 type I transmembrane pro-convertases were also fused to VP 16 Comparable to furin, PC5/6B and PC7 have previously ... [37–39] and therefore could be involved in the intracellular traf c of MT1-MMP AAA573 to the cell surface It would be interesting to assess the role of GRASP65 with respect to MT1MMP trafﬁcking to the ... transmembrane and cytoplasmic domains (amino acids 283–582) was ampliﬁed by PCR and cloned downstream of EYFP VP 16- PC5/6B, VP 16- PC7 and VP 16- furin were generated by fusing VP 16 to ICDs of mouse PC5/6B (amino...

18
603
0

Tài liệu Báo cáo khoa học: Enzymatic properties of wild-type and active site mutants of chitinase A from Vibrio carchariae, as revealed by HPLC-MS pptx

Ngày tải lên : 20/02/2014, 01:20

... oligonucleotide sequences were 5¢-CAGCCGCCGTAGAAGTT GTAAGTCATCGCAAAG-3¢, 5¢-CGCCGCCACCAGGG AACATCCAGTCAATATCTAC-3, and 5¢-GCCGCCAC CAGGGAATTGCCAGTCAATATCTAC-3¢, respectively Conﬁrmation of the mutated ... (GlcNAc )6 h, (GlcNAc)2; n, (GlcNAc)3; ·, (GlcNAc)4; ,, (GlcNAc)5; and s, (GlcNAc )6 The inset schematically shows the chitooligomers with the proposed cleavage sites (.) The GlcNAc units at the ... This corresponds to three different isomers (e.g GlcNAc.GlcNAc.GlcN, GlcNAc.GlcN GlcNAc, and GlcN.GlcNAc.GlcNAc), in accordance with the location of three acetyl moieties The signal -to- noise ratio...

11
592
0

Báo cáo khoa họcRe-engineering the discrimination between the oxidized coenzymes NAD+ and NADP+ in clostridial glutamate dehydrogenase and a thorough reappraisal of the coenzyme speciﬁcity of the wild-type enzyme docx

Ngày tải lên : 06/03/2014, 00:20

... spectrometric identication of NAD+ peak Isolation and mass spectroscopic analysis conrmed the identity of the contaminant Comparison with the spectrum of an authentic NAD+ sample revealed total ... site-directed mutagenesis and X-ray crystallography Biochem J 385, 7583 Banta S, Swanson BA, Wu S, Jarnagin A & Anderson S (2002) Alteration of the specicity of the cofactorbinding pocket of Corynebacterium ... Biol Chem 268 , 465 6 466 0 34 Valero E, Varon R & Garc a-Carmona F (1995) Kinetic study of an enzymic cycling system coupled to an enzymic step: determination of alkaline phosphatase activity Biochem...

9
526
0

Báo cáo khoa học: Molecular dynamics structures of peptide nucleic acidÆDNA hybrid in the wild-type and mutated alleles of Ki-ras proto-oncogene ppt

Ngày tải lên : 07/03/2014, 21:20

... a (C6 ¢-N1¢ -C2 ¢ -C3 ¢), b(N1¢ -C2 ¢ -C3 ¢-N4¢), c( C2¢ -C3 ¢N4¢ -C5 ¢), d (C3 ¢-N4¢ -C5 ¢ -C6 ¢), e(N4¢ -C5 ¢ -C6 ¢-N1¢), n (C5 ¢ -C6 ¢-N1¢ -C2 ¢), v1 (C8 ¢ -C7 ¢-N4¢ -C3 ¢), v2(N9 ⁄ N1 -C8 ¢ -C7 ¢-N4¢) and v3 (C4 ⁄ C2 -N9 ⁄ N1 -C8 ¢ -C7 ¢) ... conformation of the PNA scaffold is governed by six backbone torsion angles, a (C6 ¢-N1¢ -C2 ¢ -C3 ¢), b(N1¢ -C2 ¢ -C3 ¢-N4¢), c( C2¢ -C3 ¢N4¢ -C5 ¢), d (C3 ¢-N4¢ -C5 ¢ -C6 ¢), e(N4¢ -C5 ¢ -C6 ¢-N1¢) and f (C5 ¢ -C6 ¢-N1¢ -C2 ¢) These ... mutation, occurring in codon 12 of the Ki-ras proto-oncogene, replaces GGT with GAT [32] (capped region in Scheme 1) in one of the alleles of pancreatic cells This leads to pancreatic cancer [32],...

16
382
0

Báo cáo khoa học: Insertion of the plant photosystem I subunit G into the thylakoid membrane In vitro and in vivo studies of wild-type and tagged versions of the protein doc

Ngày tải lên : 07/03/2014, 21:20

... underlined) or 5¢-TGGAGCCACCCGCAGTTCGAAAAAGAA GCTGGAGATGATCGTGCT-3¢ (Strep-tag underlined), then a secondary ampliﬁcation with primers 5¢-CACCAC CACCACCACCACGAAGCTGGAGATGATCGTGCT-3¢ (His-tag underlined) ... 5¢-GCGGAGCTCAT GGCCACAAGCGCATCAGC-3¢ and 5¢-GCGGCATGCT CAGTGGTGGTGGTGGTGGTGTCCAAAGAAGCTTG GGTCGTAT-3¢ (His-tag underlined); PSAG with a C- terminal Strep-tag (PSI-G-StrepTerm), 5¢-GCGGAGCTCAT GGCCACAAGCGCATCAGC-3¢ ... GGCCACAAGCGCATCAGC-3¢ and 5¢-GCGGCATGCT CATTTTTCGAACTGCGGGTGGCTCCATCCAAAGAA GCTTGGGTCGTAT-3¢ (region encoding the Strep-tag, WSHPQFEK [19], underlined) Constructs containing a His- or Strep-tag in the...

9
422
0

Báo cáo Y học: Overexpression of a recombinant wild-type and His-tagged Bacillus subtilis glycine oxidase in Escherichia coli pptx

Ngày tải lên : 08/03/2014, 16:20

... and the following oligonucleotides: YJBr-up (5¢-GCCATGAATTC GCGCTATGAAAAGGCATTATGAAGCAGTGG-3¢) derived from the 5¢ end and YJBr-down (5¢-CCGAT GAATTCCATCATATCTGAACCGCCTCCTTGCG-3¢) derived from the ... interacts with the a-carboxylate of the D-amino acid in DAAO [15, 16] is conserved in GO (R302, see Fig 2C) On the other hand, and according to the sequence comparison, M 261 in GO appears to be located ... speci c activity) was obtained at the same concentration of inducer (data not shown) A comparison of the amount of immunoreactive GO in the total cell with the amount of GO in the crude extract...

8
482
0

Báo cáo khoa học: Combined use of selective inhibitors and ﬂuorogenic substrates to study the speciﬁcity of somatic wild-type angiotensin-converting enzyme docx

Ngày tải lên : 16/03/2014, 13:20

... for each ACE species, the catalytic efficiency of the free enzyme corresponds to the sum of the catalytic efficiencies determined for the N-domain and C- domain in the presence of inhibitor These ... inhibitor profiles (Tables and 2) validates the use of selective inhibitors as convenient tools to study the specificity of the somatic form of ACE Thus, with this approach, the catalytic property of ... different ACE species are conserved, except for RXP407 af nity for the N-domain of rat ACE (see below) Catalytic efficiencies of the ACE N-domain and C- domain in cleaving the Mca-Ala and Mca-BK(1)8)...

10
499
1

Báo cáo khoa học: Probing the active site of Corynebacterium callunae starch phosphorylase through the characterization of wild-type and His334ﬁGly mutant enzymes pot

Ngày tải lên : 23/03/2014, 07:20

... indicated in bold, respectively: XagI-for, 5¢-GGGAACTCTGCGCCT GTGGAAGGC-3¢; Eco91I-inv, 5¢-CTCATCCAGATCG GTTACCCAATC-3¢; H334G-for, 5¢-TACACCAACGGAA 5112 CCGTGCTCAC-3¢; H334G-inv, 5¢-GTGAGCACGGTTC ... from protons of the protein to protons of the bound ligand that are in close contact with the protein Relative STD effects within a given ligand therefore provide a characteristic ﬁngerprint of nonpolar ... deﬁnite conclusions about the catalytic mechanism of glycogen phosphorylases and the exact function of the PLP cofactor in it are still elusive [2 6] Figure shows that an active site His has a central...

11
430
0

Báo cáo khoa học: Alternative substrates for wild-type and L109A E. coli CTP synthases Kinetic evidence for a constricted ammonia tunnel doc

Ngày tải lên : 23/03/2014, 13:20

... prepared stock solution was stored on ice and added cold to each assay This protocol was necessary to minimize cyclization of Gln-OH with concomitant production of NH2OH (see above) The ionic strength ... Biochem 271) 4207 are substrates for CTPS from Ehrlich ascites tumour cells [45], and NH2OH has been shown to be a substrate for E coli [ 46] and Lactococcus lactis [47] CTP synthases With the ... prepared and the ionic strength was adjusted to 0.30 M using KCl At various times (5, 7, 16, 26, 36, and 46 min) the 1H NMR spectrum was recorded The relative concentrations of Gln-OH and 2-pyrrolidone5-carboxylic...

9
404
0

Báo cáo khoa học: Regulatory feedback loop between NF-jB and MCP-1-induced protein 1 RNase pdf

Ngày tải lên : 29/03/2014, 22:21

... F4 CCG CTCGAG AGCAGGAAGGGGCGAGGCAGC F11 CCC AGATCT CCCTGTGGAGAGAAGCCTGTCC F14 CCG CTCGAG AGGCAGCCCCGCCCCCGGG R2 CCC AGATCT GCCACTGATAGCTCAGACTCCTG R4 CATTCCTGTGCTGGGGGAT R6 GCTACATGAGGCTGGACACT ... R7 CCC AAGCTT TTGCATATGATGGGGGGGCTAGC R8 CCC GGATCC AAGCTT GCTGGGAGGGAGAGGACAGGG R9 CCC AAGCTT TCCATGGGGCCGAGTCCTGGG EnhAF1 CCG GGTACC TGAGAAGCAGAGCCACGCACCC EnhAR1 CCG CTCGAG CCAGCTAGGCTGCTCCTGCCC ... AGCCCAGCTTCCGGAAGAAGTCC raceF2 GGACACTGACATGGACTGAAGGAGTA raceR2 CCCAGATCTGCCACTGATAGCTCAGACTCCTG Primers used for constructs preparation F2 CCG CTCGAG CTCCAGCGTGTGGGCTCTGTG F3 CCG CTCGAG CCGTCCGCACCTCGGTCAGTG...

14
252
0

Báo cáo khoa học: The relationship between thermal stability and pH optimum studied with wild-type and mutant Trichoderma reesei cellobiohydrolase Cel7A ppt

Ngày tải lên : 31/03/2014, 07:20

... shaped active site architecture allows the direct access of the enzyme to the glucan chains of cellulose There have been suggestions that the surface loops that form the active site tunnel can open ... theoretical studies of the bellshaped curve, with Cel7A having an optimal thermostability between pH 3.5 and 5 .6 (Tm ¼ 62 ± C) This pH area coincides with the highest enzymatic activity of Trichoderma ... up to 80 C and measuring the change in intrinsic protein fluorescence as described earlier by Boer et al [11] The significant temperature dependency of the baseline fluorescence, which leads to the...

8
422
0

Báo cáo sinh học: " In vitro permissivity of bovine cells for wild-type and vaccinal myxoma virus strain" pot

Ngày tải lên : 18/06/2014, 18:20

... cell lines were tested for MYXV permissivity: KOP-R cells (RIE 244, CCLV Federal Research Centre for Virus Diseases of Animals, Island Riems), BT cells (ATCC CRL-1390) and MDBK cells (ATCC CCL-22) ... Each cell line was infected at a multiplicity of infection (m.o.i.) of and cultured for 72 h Then, infected cells were lysed by three freeze/thaw cycles One fifth of each cell lysate was inoculated ... cell surface throughout the experiment but very few infected cells This result indicates that MYXV can bind to the cell surface, but enters the cells with low efficiency The second blocking event...

5
469
0

báo cáo hóa học: " Hypoxia-inducible factor-1 (HIF-1) is involved in the regulation of hypoxia-stimulated expression of monocyte chemoattractant protein-1 (MCP-1/CCL2) and MCP-5 (Ccl12) in astrocytes" pdf

Ngày tải lên : 19/06/2014, 22:20

... the promoter regions of MCP-1 and MCP-5 genes MCP-1: GenBank Accession # AY3572 96 5'-GACCATCCAAGCAGACGTGGTA CCCACAGTCT TGCTTTAACG CTACTTTTCC AAGATAAGGT GACTCAGAAA AGGACAAGGG GTGAGCCCAA CCACACAGCTGC-3' ... pGL3/MCP1w carrying the wildtype sequence was sequenced to confirm accuracy A mutant sequence (5'-AAGCAGATTTGGTACCCTTAGTCTTGCTTTAACGCTACTTTTCC AAGATAAGGT GACTCAGAAA AGGACAAGGG GTGAGCCCAA CCACAAGGCTGCT-3') ... TGC TGT CTC A-3' 5'-GAT CTG AGA CAG CAC GTA GGG C- 3' 5'-GCTCGCTCAGCCAGATGCAAT-3' 5'-TGGGTTGTGGAGTGAGTGTTC-3' 5'-CCT GTG GCC-TTG GGC CTC AA-3' 5'-GAG GTG CTG ATG TAC CAG TTG G-3' 5'-GTC ACC CAC...

15
541
0

Báo cáo hóa học: " In vitro permissivity of bovine cells for wild-type and vaccinal myxoma virus strains" potx

Ngày tải lên : 20/06/2014, 01:20

... cell lines were tested for MYXV permissivity: KOP-R cells (RIE 244, CCLV Federal Research Centre for Virus Diseases of Animals, Island Riems), BT cells (ATCC CRL-1390) and MDBK cells (ATCC CCL-22) ... Each cell line was infected at a multiplicity of infection (m.o.i.) of and cultured for 72 h Then, infected cells were lysed by three freeze/thaw cycles One fifth of each cell lysate was inoculated ... cell surface throughout the experiment but very few infected cells This result indicates that MYXV can bind to the cell surface, but enters the cells with low efficiency The second blocking event...

5
597
0