c3n2h5 5bi2cl11 studied by 1h nmr and iins methods
Báo cáo y học: " Homonuclear 1H NMR and circular dichroism study of the HIV-1 Tat Eli variant" doc
... and Met; 9/9 Gly, 2/2 Ala, 16/16 Pro, 16/18 Arg and Lys, 2/2 Ile, 7/7 Val and Leu, and 3/3 Thr The homonuclear 1H NMR spectra of Tat Eli allowed sequential assignment of all 99 spin systems by ... for NMR (1 mM) were prepared in H2O/D2O [9:1] 100 mM phosphate buffer at pH 4.5 The homonuclear 1H NMR spectra were recorded on a Varian Inova 800 MHz NMR spectrometer operating at 799.753 MHz 1H ... dichroism and NMR studies and was involved in drafting and revising the manuscript, GRC helped synthesize Tat proteins, participated in the design of the study, carried out trans-activation assays and...
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Tài liệu Báo cáo khóa học: Determination by electrospray mass spectrometry and 1H-NMR spectroscopy of primary structures of variously fucosylated neutral oligosaccharides based on the iso-lacto-N-octaose core doc
... USA) Unity plus-500 (500.07 MHz 1H) , Unity-600 (599.89 MHz 1H) and Unity-800 (800.27 MHz 1H) spectrometers and processed with standard Varian software The observed 1H chemical shifts were reported ... Lea and one internal Lex determinants with the sequence: Completion of sequence assignment by 1H NMR Homonuclear NMR was carried out to verify the MS assignment of the iLNO core structures and ... Biochem 271) Ó FEBS 2004 Fig 1D and 2D 1H NMR spectra (800 MHz) of iLNO, region 5.5–3.0 p.p.m., at 15 °C Upper trace, 1H NMR spectrum; top-left half, 300-ms ROESY spectrum and bottom-right half, 140-ms...
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Báo cáo khoa học: Structural function of C-terminal amidation of endomorphin Conformational comparison ofl-selective endomorphin-2 with its C-terminal free acid, studied by 1 H-NMR spectroscopy, molecular calculation, and X-ray crystallography pot
... amidation Solution conformation by NMR spectroscopy and simulated annealing calculation The conformational features of EM2 and EM2OH, obtained by the present NMR measurements and molecular modeling calculations ... characteristics of EM2 and EM2OH in DMSO, TFE, H2O (pH 2.7 and 5.2) and DPC micelles (pH 3.5 and 5.2) Open and fold represent the conformations rS and n7 in parentheses indicate the reverse S- and numerical ... (pH 2.7 and 5.2) and DPC micelles (pH 3.5 and 5.2), and in monocationic form in water (pH 2.7) A typical difference between the EM2 and EM2OH was observed for the pH dependence of their NMR spectra...
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Tài liệu Báo cáo Y học: The solution structure and activation of visual arrestin studied by small-angle X-ray scattering pot
... fitting and analysis For a mixture of monomeric and dimeric scattering species, the radius of gyration is given by: R2 ¼ fM R2 þ fD R2 g gM gD ð7Þ where fM and fD are the mass fractions of monomer and ... each protein sample was preceded and followed by recording from the buffer alone; these buffer measurements were compared and provided a check on beam properties and the cleanliness of the cell ... mixture of monomers and dimers is: Ið0ÞTotal ¼ fM Ið0ÞM þ 2fD Ið0ÞM ð1Þ where fM and fD are the mass fractions of monomer and dimer, respectively Assuming that the monomer and dimer are the only...
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Báo cáo khoa học: Protein stabilization by compatible solutes Effect of diglycerol phosphate on the dynamics of Desulfovibrio gigas rubredoxin studied by NMR docx
... contribution to T2, and the subscripts f and s indicate fast and slow time scales, respectively Fitted residues in the presence of Model Not t Optimized parameters S S2 and se S2 and Rex S2, se and Rex 2 ... R1 and R2, and heteronuclear 1H1 5N NOE using the procedures outlined in Kay et al [38], modied to include a Watergate 3-9-19 water suppression scheme [39] Values of R1 and R2 were obtained by ... 1438414391 43 Englander, S.W & Mayne, L (1992) Protein folding studied using hydrogen-exchange labeling and two-dimensional NMR Annu Rev Biophys Biomol Struct 21, 243265 44 Englander, S.W., Sosnick,...
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Báo cáo khoa học: Structural features and dynamics of a cold-adapted alkaline phosphatase studied by EPR spectroscopy docx
... with MTSSL and the effects of spin-labeling on Tm were measured by the standard transphosphorylating assay and CD spectroscopy, respectively DTm is the difference between spin-labeled and nonspin-labeled ... Experimental procedures Cloning and mutagenesis The Vibrio AP gene was amplied by standard PCR methods from the pBAS20 (pBluescript KS+; Stratagene, La Jolla, CA, USA) plasmid [40] and transferred into ... Protein dynamics from NMR Nat Struct Biol 7, 740743 Kay LE (2005) NMR studies of protein structure and dynamics J Magn Reson 173, 193207 Englander JJ, Del Mar C, Li W, Englander SW, Kim JS, Stranz...
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Báo cáo khoa học: Interaction of gymnemic acid with cyclodextrins analyzed by isothermal titration calorimetry, NMR and dynamic light scattering doc
... common and studied CDs are a-, b- and c-CDs, consisting of 6-, 7- and 8-glucosyl units, respectively [14] CDs can be described as toroidal, hollow, truncated cones with a hydrophilic surface and ... temperatures 6156 TDS (kcalÆmol)1) c In 50 mM sodium NMR analysis NMR methods were applied for the analysis of interactions between GA and CDs Line widths of H NMR signals of GA alone were much broader ... Fig 1H NMR spectra (500 MHz) of GA in the absence or presence of CDs (A) NMR spectra of 2.0 mM GA in the presence of 2.0 mM a-CD, b-CD, and c-CD (B) The concentration dependence of c-CD on NMR...
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Báo cáo khoa học: Netropsin interactions in the minor groove of d(GGCCAATTGG) studied by a combination of resolution enhancement and ab initio calculations pot
... pyrrole rings A and B, between the guanidinium end and pyrrole ring A, and between the amidinium end and pyrrole ring B for the 10-Nt reported structure, NETRSN [31] structure and the DFT approximation ... guanidinium atom and a N2 guanine atom ˚ (N10(Nt)ÆÆÆN2(G9)) is 3.32 A In 10-DAPI and 10-Dst the contact distance between amino group and amidi˚ ˚ nium atoms and N2(G9) is 3.14 A and 3.17 A, respectively ... most similarity with GDLB31 and GDJ046 Contacts conserved in all four structures are between the N4(Nt) and O2(T28), between the N6(Nt) and O2(T7) and between the N8(Nt) and O2(T8) atoms The guanidinium...
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báo cáo khoa học: " Quantitative 1H NMR metabolomics reveals extensive metabolic reprogramming of primary and secondary metabolism in elicitor-treated opium poppy cell cultures" pptx
... http://www.biomedcentral.com/1471-2229/8/5 1H NMR Figure elicitationspectra of D2O extracts from control and elicitor-treated opium poppy cell culture collected 0, 5, 30 and 100 h post1H NMR spectra of D O extracts from control and ... Aqueous extracts of control and elicitor-treated cell suspension cultures of opium poppy were analyzed in D2O by 1H NMR Figure shows typical spectra obtained at 0, 5, 30 and 100 h post-elicitation ... analysiscollected at 80 and 100 NMR spectra for Scores Scores (A) and corresponding loadings plot (B) of orthogonal partial least-squares-discriminant analysis (OPLS-DA) on 1H NMR spectra for D2O...
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Nanostructure, biopiezoelectric and bioferroelectric behaviors of mollusk shells studied by scanning probe microscopy techniques
... [(c) and (d)] and DART-LPFM [(e) and (f)] are scanned (500× 500 nm , 256× 256 pixels); (c) and (e) are the amplitude images; (d) and (f) are the phase images Fig 5.6 DART-VPFM [(b) and (c)] and ... [(g) and (i)] and phase [(h) and (j)] images in the lateral direction All of the amplitude images show the piezoresponse at Vac Last row are the magnitude [(k) and (m)] and argument [(l) and (n)] ... 2nd and 3rd column (400× 400 nm ): observed from the interior and boundary of platelet on the nacre surface (a) (d) and (g) are height images, (b) (e) and (h) are amplitude images, and (c) (f) and...
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Effect of temperature and trace water on surface forces studied by liquid AFM
... thank you for all the support and help in getting me to keep my focus To the 1SAPians, thank you for your understanding and support, especially Glenn, CP, Michiel, Victor and Karen I would like to ... like to express my deepest gratitude to Professor Andrew Wee for his great support and advice throughout my candidature both experimentally and administratively Last but not least, to the “old ... 5.6 c -NMR results for a) Hexadecane, and b) Hexadecane with 0.005% ppm water added Inset shows a Hexadecane skeleton diagram with the corresponding c -NMR peak numbers 140 Figure 5.7 h -NMR results...
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PHỤ LỤC 1: PHỔ 1H NMR CỦA CHẤT A3
... LỤC 2: PHỔ 1H NMR CỦA CHẤT F (160 161) PHỤ LỤC 3: PHỔ 1H NMR CỦA CHẤT L (162) PHỤ LỤC 4: PHỔ 13C NMR VÀ DEPT CỦA CHẤT L (162) PHỤ LỤC 5: PHỔ 1H NMR CỦA CHẤT F2 (163) PHỤ LỤC 6: PHỔ 13C NMR VÀ DEPT ... CHẤT F2 (163) PHỤ LỤC 7: PHỔ 1H NMR CỦA CHẤT K2 (165) PHỤ LỤC 8: PHỔ 13C NMR VÀ DEPT CỦA CHẤT K2 (165) PHỤ LỤC 9: PHỔ 1H NMR CỦA CHẤT O1 (168) PHỤ LỤC 10: PHỔ 13C NMR VÀ DEPT CỦA O1 (168) ...
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Tài liệu Developing Your Intuition - By Sandy Breckenridge and Kirk VandenBerghe doc
... Developing Intuition Series By Sandy Breckenridge and Kirk VandenBerghe Inttroducttiion: Channelliing In roduc on: Channe ng Warm greetings to you My name is Kirk VandenBerghe, and I’ll be your guide ... intuition And yet if we over-contemplate them, we may never get anywhere Yes, one could go around and around in circles by over-analyzing However, we can notice and allow our fears and limiting ... take in, and be aware of, our sensory guidance by embracing our sensitivities As we develop our ability to tune in and connect with these subtle energies, we develop a most wonderful and useful...
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Tài liệu Bài 12: ICA by Nonlinear Decorrelation and Nonlinear PCA doc
... 240 ICA BY NONLINEAR DECORRELATION AND NONLINEAR PCA is a stochastic gradient algorithm and the other one a recursive least mean-square algorithm 12.1 NONLINEAR CORRELATIONS AND INDEPENDENCE ... because the functions f and g are almost arbitrary Some kind of approximations are needed This problem was considered by Jutten and H´ rault [228] Let us assume that f (y1) e and g (y2 ) are smooth ... 12.1, Cichocki e 244 ICA BY NONLINEAR DECORRELATION AND NONLINEAR PCA B B and Unbehauen proposed a feedforward network with weight matrix , with the mixture vector for input and with output = Now...
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Tài liệu JQuery: Novice to Ninja by Earle Castledine and Craig Sharkie pptx
... good, and how it complements HTML and CSS in a more natural way than our old friend and bitter enemy: plain old JavaScript We’ll also look at what’s required to get jQuery up and running, and working ... objects, and more These functions by themselves are partic ularly handy, but they help promote a seamless integration between jQuery and JavaScript which results in code that’s easier to write and ... blocks, and your friends and family will wish you’d never discovered it! On top of the core jQuery library is jQuery UI: a set of fine-looking controls and widgets (such as accordions, tabs, and...
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Tài liệu Drawing by Lauren Jarrett and Lisa Lenard- P1 docx
... meaning right hand and the dominant left brain There is such prejudice against left-handedness and the left generally—socially, politically, morally, and culturally and early conceptions and language ... Thoughts on Landscape Space 215 Tools for Landscape and Perspective 216 Seeing and Drawing the Landscape 216 Photographs: To Use or Not to Use, That Is the Question 217 The Landscape ... Fur and Feathers, Skin and Scales 260 Go Out Where They Are 261 Your Backyard and in the Neighborhood 261 Field and Stream, Mountain and Lake 263 Natural History Museums and...
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Tài liệu Drawing by Lauren Jarrett and Lisa Lenard- P2 ppt
... schoolchildren (and the adults they became) can be identified by their careful o’s and w’s—not to mention their p’s and q’s Warm-Up for the Eyes and Hand Just as you may have practiced your penmanship by ... shape, and proportion in your hand, and try to put it into your drawing Keep working until you have drawn all the lines and shapes in the palm of your hand That it won’t look like a hand doesn’t ... other hand on the table Take another good look at your hand and the lines in your palm Pick a place and a line on your hand to start with Pick a place on your paper to place your pencil and begin...
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Tài liệu Drawing by Lauren Jarrett and Lisa Lenard- P3 doc
... somewhere between close-up and still life space and leave the long views for later Understanding, seeing, and drawing from a particular view and vantage point is a big step and can seem complicated, ... pencils, charcoal, paper stomp, and conte crayons all make their own marks and tones Each comes in different thicknesses, from stubby and thick to thin and fine, and each comes in different hardnesses ... off by yourself and draw Vacations and business trips are other great drawing opportunities Planes, trains, and buses are filled with faces to try Boats are filled with interesting places and...
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