... to refractory septic shock Discussion Management of resectable bileduct adenocarcinoma Adenocarcinoma of the extrahepatic bileduct are relatively infrequent malignancies representing less than ... the extrahepatic bileduct (previously referred as extrahepatic cholangiocarcinomas) are also distinguished as proximal or distal, depending on their location along the bile ducts Surgery provides ... management of bileduct cancer Ann Surg 1990, 211:614-620 discussion 620-611 Schoenthaler R, Phillips TL, Castro J, Efird JT, Better A, Way LW: Carcinoma of the extrahepatic bile ducts The University...
... group A549 cells stably transfected with PEGFPN1-HMGN2 A549 cells stably transfected with PEGFPN1 A549 cells treated by LPS A549 cells stably transfected with pSilencer-HMGN2-2 A549 cells stably ... Descriptions PE-H group A549 cells stably transfected with PEGFPN1-HMGN2 A549 cells stably transfected with PEGFPN1-HMGN2 and then adding AA A549 cells induced by LPS A549 cells stably transfected ... Compared with A549 cells stimulated by LPS, HBD-2 expression was 50% less in HMGN2 knockdown cells and was over 30% higher in HMGN2 overexpressing cells In addition, reintroduction of HMGN2 re-expression...
... enterochromaffin cells, also known as argentaffin or Kulchitsky cells The term enterochromaffin refers to the ability to stain with potassium chromate (chromaffin), a feature of cells that contain ... (kultschitzky) cells in the human gall-bladder J Clin Pathol 1954, 7:318-321 Barron-Rodriguez LP, Manivel JC, Mendez-Sanchez N, Jessurun J: Carcinoid tumor of the common bile duct: evidence for ... Page of Kopelman D, Schein M, Kerner H, Bahuss H, Hashmonai M: Carcinoid tumor of the common bileduct HPB Surg 1996, 10:41-43 Squillaci S, Marchione R, Piccolomini M, Colombo F, Bucci F, Bruno...
... intrahepatic duct and B4 No additional intraductal tumor was found in the right intrahepatic and extrahepatic bileduct Resection margins of the proximal bileduct and hepatic parenchyma were free ... lobectomy and extrahepatic bileduct resection could be successfully performed Macroscopic examination of the resected specimen revealed a cystic dilatation of the intrahepatic bile ducts with intraluminal ... intraductal papillary carcinoma of the bileduct and revealed no stromal invasiveness (Figure 3B, 3C) The tumor was 14.0 × 13.0 cm-sized and involved the distal portion of the left intrahepatic duct...
... in the middle bileduct and main PV (Figure 1a and 1b) Positron emission tomography with fluoro-2-deoxy-D-glucose (FDG PET) scan and 3D imaging showed hot spots in the main bileduct and main ... there was a circular lesion obstructing the bile duct, with a fragile tumor thrombus cm in diameter (Figure 4a,b) Histological examination showed that bileduct cancer was a poorly differentiated ... tomography shows the PVTT (white arrow) and bileduct cancer (white dotted arrow) a and b a and b Computed tomography shows the PVTT (white arrow) and bileduct cancer (white dotted arrow) Page of...
... hepatic cyst However, the differentiation of benign and malignant bileduct strictures is not easy pre-operatively; in cases with a bileduct stricture without a demonstrable mass on CT or US, the ... intrahepatic bileduct dilatation without demonstrable mass on ultrasonography or CT: benign versus malignancy J Gastroenterol Hepatol 2003, 18:1287-1292 Babu S, Smithson J: Bileduct stricture: ... malignancy of the left hepatic duct There were mild adhesive changes around the liver that might have been caused by cholangitis No tumor lesions were found around the bileduct Only a small hepatic...
... parainfluenza after endoscopic retrograde cholangiopancreatography and open cholecystectomy with a common bileduct exploration: a case report Journal of Medical Case Reports 2010, 4:170 ... stone A CBD Ttube was placed, brought out through the lateral abdominal wall, and attached to the bile drainage bag No other stones remained in the CBD after the completion of surgery, which was ... include infection secondary to retroperitoneal perforation occurring during sphincterotomy or introduction of the bacterium from the upper airway during the ERCP and subsequently to the retroperitoneal...
... triple duct injury, with right and left hepatic duct ligaFigure common bileduct cross-section (Bismuth type V), was and bile tion revealed in magnetic resonance imaging A triple bileduct injury, ... the biliary anatomy and assess the level of injury A triple bileduct injury, with right and left hepatic duct ligation and common bileduct cross-section, was revealed (Bismuth type V, Figure 1) ... minor and major bileduct injuries has been proposed by McMahon Minor injuries include laceration of the cystic to common bileduct junction and laceration of the common hepatic duct over less...
... in L929 cells Inhibition of autophagy up-regulates apoptosis in oridonin-induced L929 cells To investigate the role of autophagy in oridonininduced apoptosis in L929 cells, we pretreated cells ... changes when the cells were cultured with oridonin for 6, 12 or 24 h As shown in Fig 1C, the majority of oridonin-treated L929 cells became round in shape at h At 12 h, some of these cells showed ... g DCF positive cells (%) a 100 80 60 40 20 0 6h 12 h 24 h Oridonin C 0h 36 h Ori + NAC 6h 12 h 36 h 24 h Fig Oridonin-induced ROS generation was blocked by NAC in L929 cells The cells were incubated...
... derivatives of superoxide also mediate signaling transduction [22] In nonphagocytic cells, such as fibroblasts, endothelial cells, vascular smooth muscle cells, cardiac myocytes and thyroid tissue, ROS ... [51] Recently, the production of IL-6 by dendritic cells has been reported [52] Our parallel study also observed the formation of a large amount of IL-6 when dendritic cells were sorting from ... CD4+ and CD8+ T cells Infect Immun 75, 260–269 Pontoux C, Banz A & Papiernik M (2002) Natural CD4 CD25+ regulatory T cells control the burst of superantigen-induced cytokine production: the role...
... have suggested that inhibition of caspase-3 activity can block induction of apoptosis by Ab in primary neuronal cells and PC12 cells [14] The neurotoxicity of Ab seems to depend on its ability ... using PC12 cells Using a phase-contrast microscope, we observed significant morphological changes of PC12 cells treated with Ab(25–35) and caspase-3 inhibitors after 48 h (Fig 3A) In cells treated ... even at 40 lm Apoptotic cells with degraded DNA appear as cells with hypodiploid DNA content and are represented by the so-called sub-G1 peaks on DNA histograms After PC12 cells had been treated...
... epidermal 3D model is a relevant skin cellular model, complementary to isolatedcells In this study, we used human cutaneous cells and hRE to test the hypothesis that exposure to RFR results in ... detection in human primary epidermal and dermal cellsCells were analysed by flow cytometry using ANX–FITC ⁄ PI The percentage of viable, apoptotic and necrotic cells was determined by quadrant analysis ... dermal fibroblast (NHDFe) cells after GSM-900 exposure did not vary compared with sham-exposed cells Similar results were obtained for the percentage of necrotic versus viable cells (n ¼ 5) UVB radiation...
... and CM cells Total cell extracts were prepared from cells treated with 10 lM hA or rA at the indicated time-points and subjected to western blot analysis using anti-p-p38 kinase IgG Fold induction ... b-cell apoptosis We detected a further reduction in caspase-3 activation and apoptosis in cells pretreated with combined SB203580 and JNK inhibitor I (70% reduction in total), and full suppression ... RINm5F cells after hA treatment (data not shown) However, no increment in p-ATF-2 level was detected in cells treated with either vehicle alone or noncytotoxic rA in either RINm5F or CM cells, ...
... Rex-1 gene, primer sets span introns Day cells are composed of ES cells and MEF cells grown in ES media containing leukemia inhibitory factor (LIF) These cells express the Rex-1 and b-actin genes ... ES cells and in human hematopoietic progenitor cells Discussion B Fig Transcription of LCR hypersensitive site in undifferentiated murine embryonic stem cells and in human CD 133+ bone marrow cells ... fetal liver cells These results indicate acetylation plays a critical role in the transcriptional potentiation and developmental regulation of these genes in progenitor cells or cells that have...
... protein level in HEK293 cells The test cells were transfected with Rz-1 and Rz-2 encoding plasmids and 36 h postincubation the protein fraction was isolated from the cells and subjected to Western ... HEK293 cells Fig Expression of pUC-KE-tRNA-CTE-Rz-1 and Rz-2 plasmids in HEK293 cells determined by semiquantitative RT/PCR Ribozyme encoding plasmids were transiently transfected into the cells ... products was determined under co-amplification conditions 36 h after transfection An agarose gel electrophoresis of the RT/PCR products demonstrates that the level of the 150-bp control product...
... CHO-K1 cells by the lipofection method using FuGene reagent (Roche, Basel, Switzerland) Cells were incubated 40 h after transfection and observed by a FLIM microscope The transfected cells were ... by FRET FLIM measurements were conducted on the live cells at 37 °C after the culture medium was replaced with fresh medium The emission lifetimes of fluorescent cells were measured on an inverted ... is shown in Fig 1B Its lifetime was fairly constant throughout the cells, and similar lifetimes were observed in different cells expressing the fluorescent protein (Fig 1B) Figure 1C,D shows a...
... epidermal melanocytes (NHEM), HMV-II melanoma cells, and HeLa cells The EDNRB promoter is heavily methylated in HeLa cells, but not in NHEM and HMV-II cells (Fig 8A) Subsequently, we examined whether ... in NHEM and HMV-II melanoma cells (Fig 2B) Three protected fragments of 436, 407, and 228 bases were detected in NHEM and HMV-II cells but not in HeLa cervical cancer cells The two fragments of ... in melanocyte-lineage cells We first analyzed the promoter activity of the EDNRB gene by transient transfection assays in HMV-II melanoma cells and HeLa cervical cancer cells (Fig 3A) The deletion...
... differences in the method used to prepare the isolated domains Dioum et al prepared the isolated domains by denaturation of an insoluble suspension of E coli cells overexpressing the protein and then ... Discussion In this study, we characterized the isolated hemebound PAS-B domain of NPAS2 and identified His335 as one of the axial ligands We found that the isolated PAS-B domain from NPAS2 forms a large ... separate studies, we have found that the heme-binding affinity of the isolated PAS-A domain is much higher than that of the isolated PAS-B domain (our unpublished results) We speculate therefore...
... epithelial cells of the secondary lamella including the chloride cells and pavement cells whose nuclei are labeled by arrows and double arrowheads, respectively, in Fig 9D The nuclei of the pillar cells ... demonstrated that the chloride cells can be classified into two types based on the locations in the gill: filament chloride cells and lamellar chloride cells The lamellar chloride cells are considered to ... FEBS 2002 Fig Western blot analysis of eRBCC protein expressed in COS-7 cells COS-7 cells expressing eRBCC or mock transfected cells were solubilized with the Laemmli buffer and analyzed by Western...
... to regulation by bile acids in human hepatoma cells Cholic acid and chenodeoxycholic acid (CDCA) are two major primary bile acids detected in human bile [19– 21] The effects of bile acids on HBV ... enhancers To determine whether bile acid-induced SHP expression affects the induction of HBV gene expression by the bile acid-induced FXRa pathway, Chang liver cells were transfected with the ... either from a functional defect of bile secretion, or from an obstruction of the bileduct [38] Under cholestatic conditions, hepatocytes are exposed to increased bile acid concentrations, resulting...