... 1.16 or a vector of 1.16 at 180 ° By taking the square root of 0.752 plus 1.162, we get a resultant of 1.38 oz-in Now to get the proper angle: ^4 = 180 ° - tan-1 ^ - 180 ° - 32.8° - 147.2° So we ... Another imbalance of oz-in exists at station 3, located in from the right end, and at an angle of 180 ° from the same reference We want to statically and dynamically balance the rotor, by means of ... 90° The imbalance at station 3, when sensed at station 1, is X 5A2 = % = 0.83 or a vector 0.83 at 180 ° By taking the square root of 2.252 plus 0.832, we find that we must remove 2.41 oz-in Now at...
... provides new insights into protein folding, dynamics and structure Trends Biochem Sci 26, 612– 618 18 Balny, C., Masson, P & Heremans, K (2002) High pressure effects on biological macromolecules: ... in Fig according to (Eqn 3) and normalized by dividing by the number of subunits in the capsid (180 ) DV/n Changes in secondary structure upon dissociation and denaturation To further confirm the ... higher urea concentrations, both WT bacteriophage and the D11N mutant lost the ellipticity at 218 nm, indicating complete denaturation (results not shown) the data of Fig (Eqn 3) M88V and T45S...
... substrate phosphate Acknowledgements The nancial support from the Austrian Science Funds (P15 118 and P 1189 8 to B.N.) is gratefully acknowledged We thank Dr Dieter Palm for communicating a protocol ... materials Recombinant CcStP and site-directed mutants thereof were produced as described elsewhere [15 ,18] Natural CcStP was puried by a reported procedure [16] If not stated otherwise, recombinant CcStP ... according to Eis et al [19] Analytical enzymes and enzyme substrates were specied in previous papers [1 518] All other chemicals were of reagent grade and obtained from Sigma and Fluka Preparation of apo-Cc...
... Mammalian 1-amino acid decarboxylases 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 producing 1,4-diamines: analogies among differences Trends Biochem Sci 19, 318 319 Engel, N., Olmo, M.T., Coleman, C.S., ... flexible region around Arg334 is required during the transaldimination process Protein Sci 7, 180 2 181 0 38 Bertoldi, M., Gonsalvi, M., Contestabile, R & Voltattorni, C.B (2002) Mutation of tyrosine ... twofold axial symmetry dimers similar to those described for the crystal structures of pig DDC [18] Nevertheless, it predicts a more occluded catalytic center when compared with the DDC crystal...
... 2.9 18. 0 ± 2.0 80.0 ± 3.5 46.0 ± 2.2 12.2 ± 2.5 68.0 ± 2.9 50.0 ± 3.0 20.0 ± 2.2 89.0 ± 3.5 82.0 ± 3.0 17.0 ± 2.0 99.0 ± 3.0 Certified values of NIST 1568a (0.29 ± 0.03 g g−1 As) and DORM-2 (18. 0 ... extractions ¯ Sample (total content, mg kg−1 ) Extraction efficiency number of extraction Water Rice (0 .182 ± 0.031) 1st 2nd Extractions n = Chicken (0.168 ± 0.002) Methanol:water M H3 P04 1:1 9:1 1:1–9:1 ... ml of 1:1 methanol: water were added following a similar treatment performed by Shibata et al [18] The mixture was maintained at 55 ◦ C for 10 h and then treated in an ultrasonic focalized bath...
... real sectors: a critical survey of the literature”, BCBS Working Papers, no 18, February 2011 (www.bis.org/publ/bcbs_wp18.htm) This is further elaborated in Committee on the Global Financial System, ... specific difficulties that are likely to be faced by EMEs in the implementation of Basel 3? 16.30 18. 30 Session II on “Implications of the Evolving Regulatory Framework for Equity in the post crisis ... financial system Some have expressed concerns that strengthening bank capital could slow growth 18 H Hannoun, “Sovereign risk in bank regulation and supervision: where we stand?”, speech to the...
... bottom): 2, 5, 7, 10, 15, 20, 30, 45, 60, 75, 120, 150 and 180 lM The symbols in (B) correspond to various concentrations between and 180 lM at four different temperatures: 10, 15, 20 and 25 °C ... counterparts [4 ,18] An inspection of the three-dimensional structure of the wild-type protein (Fig 1) reveals the existence of a salt bridge between Glu34 in one chain and Lys13 in the other [18] , an ... dependence for Cp,N2 and a quadratic one for Cp,U, as described elsewhere [26] Cp;N2 ¼ aN þ bN ÁT Eqn (18) Cp;U ¼ aU þ bU ÁT þ cU ÁT2 Eqn (19) Parameters bU and cU were obtained from the nonlinear quadratic...
... (residues 2–50) All heavy atoms (residues 2–50) Backbone atomsb (residues 2–11; 18 50) All heavy atoms (residues 2–11; 18 50) Ramachandran plot analysis (%)c Residues in most favoured regions Residues ... Nature 349, 178 180 Wojciak, J.M., Sarkar, D., Landy, A & Clubb, R.T (2002) Armsite binding by lambda-integrase: solution structure and functional Ó FEBS 2003 10 11 12 13 14 15 16 17 18 19 20 21 ... coilÕ conformation, are not shown The two a-helices – a1 (residues Leu5–Arg11) and a2 (residues Leu18–Glu27) – are shown in red and yellow; the loop between them is indicated by L The five b-strands...
... (1998) A conserved C-terminal assembly region in paramyosin and myosin rods J Struct Biol 122, 180 187 27 Engel, J & Schwarz, G (1970) Co-operative conformational transitions of linear biopolymers ... Wada, Y & Morishima, I (1994) ÔModuleÕ substitution in hemoglobin subunits J Biol Chem 269, 187 50 187 56 Ó FEBS 2002 Thermodynamics of segmented coiled coil protein (Eur J Biochem 269) 841 35 ... solution of 0.01 M Na phosphate buffer (pH 8.0) and 0.15 M NaCl (a) Spectra of the SM4 fibritin (182 residues per monomer) were registered at 298, 335, and 358 K The protein has the native conformation...
... Dobson, C.M & Smith, L.J (2001) Amyloid fibril formation by a helical cytochrome FEBS Lett 495, 184 186 Tomlinson, E.J & Ferguson, S.J (2000) Conversion of a c type cytochrome to a b type that spontaneously ... Cytochromes c555 from the hyperthermophilic bacterium Aquifex aeolicus Ó FEBS 2002 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 Cytochrome c structure and stability (Eur J Biochem 269) 3361 (VF5) Characterization ... periplasm [17] DsbD contains a domain with potential disulfide isomerase activity facing the periplasm [18 20] Other Dsb proteins, DsbA and DsbB, which have been determined to oxidize cysteine thiols...
... (1998) Binding of Ca2+ and Zn2+ to human nuclear S100A2 and mutant proteins J Biol Chem 273, 188 26 188 34 18 Koch M, Diez J & Fritz G (2006) Purification and crystallization of the human EF-hand tumour ... S100A13, a key component of the FGF-1 nonclassical copper-dependent release complex Biophys J 91, 183 2– 184 3 29 Baudier J, Glasser N & Gerard D (1986) Ions binding to S100 proteins I Calcium- and zinc-binding ... contributes to human pancreatic cancer pathogenesis and progression Proc Natl Acad Sci USA 104, 186 36 186 41 32 Taylor KM, Morgan HE, Smart K, Zahari NM, Pumford S, Ellis IO, Robertson JF & Nicholson...
... FAD (0.2 mM) in the activity assay mixture 506 d (334) (342) (338) (342) (338) (340) (337) (341) 18. 9 9.4 12.3 These values were determined in the presence FEBS Journal 273 (2006) 504–512 ª 2006 ... dimeric state to the thermal stability of the flavoprotein d-amino acid oxidase Protein Sci 12, 1 018 1029 Piubelli L, Caldinelli L, Molla G, Pilone MS & Pollegioni L (2002) Conversion of the dimeric...
... from the E coli strain A19 (E coli Genetic Stock Center CGSC) in a procedure modified after Zubay [18] The cells were washed in washing buffer [10 mM Tris-acetate, pH 8.2, 14 mM Mg(OAc)2], with mM ... of s The data pitch was 0.2 nm and the scanning rate 50 nmÆmin)1 The spectra were recorded from 188 to 260 nm The presented data are the average of three scans and smoothed by means-movement with ... copper blades used as sample holders and then frozen by plunging into liquid ethane cooled to )180 °C by liquid nitrogen Freeze-fracturing was performed in a Balzers 400T freeze-fracture apparatus...