building the machines scaffolding protein functions during bacteriophage morphogenesis
Báo cáo khoa học: Structure–function analysis of the filamentous actin binding domain of the neuronal scaffolding protein spinophilin pot
... involved in the regulation and maintenance of dendritic spine morphology is fundamental to our understanding of the functions of the central nervous system The molecular details that underlie the regulation ... verify the in silico predictions, we carried out one-dimensional 1H NMR experiments (Fig 2A,B) The NMR spectra of these constructs perfectly resembled the spectra of unfolded proteins: they showed ... Stroke to WP The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institute of Neurological Disorders and Stroke or the National...
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Tài liệu Báo cáo khoa học: Nucleolin/C23 mediates the antiapoptotic effect of heat shock protein 70 during oxidative stress pptx
... accompanied by the appearance and an increase in the 80 kDa fragment (Fig 2) Although both the untransfected cells and the cells transfected with the vector alone started to show the 80 kDa fragment ... in cells transfected with the vector alone and cells transfected with Hsp70 cDNA during H2O2 exposure, the cells were treated with H2O2 for h Protein synthesis was then inhibited by cycloheximide ... in H2O2-treated cells transfected with the vector alone; the half-life of the protein was estimated to be less than h (Fig 3, left panel) At the same time, the 80 kDa degraded product was detected...
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Báo cáo Y học: Synthesis and turn-over of the replicative Cdc6 protein during the HeLa cell cycle potx
... and that about one half of the newly synthesized hCdc6p associated with chromatin during the 2-h label period Rates of hCdc6p synthesis To investigate whether the synthesis of hCdc6p was restricted ... Mcm loading during G1 phase, it is certainly not sufficient because the Cdt1 protein is also involved, and the Cdt1 protein is effectively sequestered during S phase by the regulator protein geminin ... attacks the phosphorylated form of hCdc6p However, more importantly in the present context, we note that the synthesis of hCdc6p continues through most of the cell cycle Synthesis of hCdc6p during...
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Báo cáo khoa học: Identification of mitogen-activated protein⁄extracellular signal-responsive kinase kinase 2 as a novel partner of the scaffolding protein human homolog of disc-large docx
... with the endoplamic reticulum and microtubules, hDlg and MEK2 may together regulate the trafficking of newlysynthesized proteins to the cell periphery Therefore, a better understanding of the role ... the GLGF sequence in either the PDZ1 domain (PDZ-1mut-2) or the PDZ2 domain (PDZ-1-2mut), or the hDlg PDZ3 domain, all fused to the GAL4 DNA-binding domain, were co-expressed in yeasts with the ... Discussion In the present study, we show, for the first time, the expression of hDlg in the human vascular cell population, which is the most abundant in arterial wall tissue (i.e the hVSMCs) This protein...
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Báo cáo khoa học: Characterization of the interactions of the nephrin intracellular domain Evidence that the scaffolding protein IQGAP1 associates with nephrin potx
... needed to organize and maintain the slit diaphragm They also indicate how important the elucidation of the protein protein interactions formed by nephrin are for the 229 Intracellular domain of ... extra residue, a methionine residue, to the N-terminus of the recombinant protein To generate the constructs for GST–nephrin fusion proteins, the cDNA encoding the fulllength cytoplasmic domain of ... Utilizing recombinant nephrin proteins, we analysed the requirements for the interactions with the known intracellular partners Furthermore, we identified IQGAP1, an effector protein of small GTPases...
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Báo cáo khoa học: Dissecting the role of protein–protein and protein–nucleic acid interactions in MS2 bacteriophage stability potx
... DNA On the other hand, when we incubated the coat protein with tRNA or the hairpin sequence, neither was able to protect the protein against denaturation (Fig 7A) After pressure release, the value ... of the probe when the protein was subjected to high pressure, but the DNA could not protect the protein dimer against dissociation The 1470 Assembly of infectious bacteriophage MS2 requires the ... capsid protein The only feasible explanation is the formation of complexes of folded capsid protein with the small RNAs that compose the VLPs [11] The presence of maturase protein in authentic...
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Báo cáo khoa học: Shedding of the amyloid precursor protein-like protein APLP2 by disintegrin-metalloproteinases Retinoic acid-induced upregulation of substrate and proteinase ADAM10 during neuronal cell differentiation ppt
... Probably therefore the effects on the a-like cleavage of APLP2 by metalloproteinase inhibitors (Fig 2) or on the b-like cleavage by a BACE-1 inhibitor (Fig 3) are probably not as strong as for the ... characterization of both cell lines during differentiation see Voigt and Zintl [42] The effect of RA-induced differentiation on either the substrate APLP2 or the proteinase ADAM10 was analyzed using ... in the amount of BACE-1 mRNA (Fig 6B and 147% of control) At the protein level, the enhancement of APLP2 and ADAM10 was confirmed for both cell lines (Fig 7A,B) Again in the N-type SH-SY5Y the...
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Báo cáo khoa học: Identification of alternative promoter usage for the matrix Gla protein gene Evidence for differential expression during early development in Xenopus laevis doc
... amplification of the larger IA transcript; b + c for amplification of the shorter IB transcript (B) PCR amplification of the two specific transcripts and of the ODC gene from the same RT reaction (C) Southern ... activity is consistent with the other results obtained, namely the intensity of the RT-PCR bands and the weak in situ hibridization signal at stage 12 Injection of the )949LuC, +2733 ⁄+3013LuC ... transcription of the zygotic MGP gene is directed by nuclear factors binding to the distal promoter These results were further corroborated by the results obtained after radial microinjection into the marginal...
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Báo cáo sinh học: " The herpes simplex virus UL20 protein functions in glycoprotein K (gK) intracellular transport and virus-induced cell fusion are independent of UL20 functions in cytoplasmic virion envelopment" docx
... encoding glycoprotein B (gB) [13,14], and the UL53 gene coding for glycoprotein K (gK) [15-19] Of these four membrane associated proteins, only UL20 and gK are absolutely essential for the intracellular ... on the first amino acid mutated in each cluster Single amino acid replacements are indicated with the amino acid position bracketed on the left by the targeted amino acid and on the right by the ... envelope proteins from plasma membranes to the TGN [32-36] The acidic cluster motifs appear to direct TGN localization by binding to a cellular connector protein, PACS-1, which connects the glycoproteins...
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Báo cáo sinh học: "The phosphatidylserine receptor has essential functions during embryogenesis but not in apoptotic cell removal" pot
... cells These include the serum proteins 2-glycoprotein and protein S [20,21], the growth-arrest-specific gene product GAS-6 [22], complement activation products [23], the milk fat globule protein ... (MFG-E8) [17,18] The exposure of the phospholipid phosphatidylserine (PS) in the outer leaflet of the plasma membrane of apoptotic cells has been described as one of the hallmarks of the induction ... the branchial arches, the developing limbs, the heart, the primitive gut and the developing eye (c-e) Sectioning of E12.5 -galactosidase-stained embryos confirms expression of Ptdsr in (c) the...
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Báo cáo y học: "The use of moderate hypothermia during cardiac surgery is associated with repression of tumour necrosis factor-α via inhibition of activating protein-1: an experimental study" doc
... hypothermia during CPB represses the expression of TNF-α in the myocardium by selective inhibition of the p38 MAPK/AP-1 pathway In a similar in vivo model, we previously showed that hypothermia during ... concentration: hypothermia versus normothermia Shown are the expression of TNF-α mRNA and TNF-α connormothermia centrations in pigs operated on under moderate hypothermia (28°C; circles) or normothermia ... moderate hypothermia than in the other animals (Figure 1a,b) Six hours after CPB, concentrations of TNF-α were lower in animals operated on under moderate hypothermia than in the others (P < 0.05;...
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Functions of the dynamin like protein VPS1 in actin organization in saccharomyces cerevisiae
... factors During the CCV formation, AP-2 complex brings clathrin to the PM and also interacts with the cytoplasmic domains of some receptors, thereby concentrating the cell surface proteins to the ... clathrin, there are several clathrin accessory proteins which are able to assist the formation of CCVs in vivo The details of these accessory proteins are given below AP-180 and CALM The neuronal protein ... vacuolar protein sorting 145 7.1 Roles of Vps1p in the protein trafficking at the trans-Golgi network (TGN) 150 List of Tables xii LIST OF TABLES Table: Dynamin-like proteins and their proposed functions...
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Thụ thể hormon adrenalin, Protein G và các chất truyền tin thứ hai
... protein G hot hoỏ protein ny Khi protein G c hot hoỏ s tip cn vi tác với enzym adenylate cyclase G mng t bo to phn ng bin i ATP thnh AMP protein nm vũng Quỏ trỡnh ny c minh ho s hỡnh 6.2 Protein ... protein b bao võy bng mt trỡnh t thớch hp c chp nhn Protein kinase A v protein kinase G nhn c cỏc trỡnh t khỏc thớch hp cho nú Vỡ vy, cỏc enzym ny ó iu ho nhng protein khỏc 6.2.6 S iu ho ca protein ... kt vi protein G (GPCRs) Ba tiu n v to protein G l: G mang v trớ gn nucleotit, cú ớt nht 20 loi G khỏc c tỡm thy cỏc t bo ng vt cú vỳ Tiu n v G v G 74 6.2.4.1 C ch hot ng ca protein G Protein...
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Nghiên cứu sử dụng protein bột nhân hạt cao su thay thế một phần protein bột cá trong khẩu phần ăn của cá rô phi (oreochromis niloticus) giai đoạn thương phẩm nuôi trong phẩm nuôi trong bể
... 5% protein bột cá công thức CT1 5% protein bột nhân hạt cao su + Công thức III (CT3): Thay 10% protein bột cá công thức CT1 10% protein bột nhân hạt cao su + Công thức IV (CT4): Thay 15% protein ... ăn tự nhiên phong phú nhu cầu hàm lợng protein phần cá giảm Đã có nhiều nghiên cứu hàm lợng protein phù hợp phần cá Rô phi Theo Jauncey Ross (1982), phần protein tối u cho cá Rô phi bột 50%; cỡ ... nh protein, lipid, lợng, vitamin khoáng Nhu cầu thay đổi tuỳ thuộc loài cá giai đoạn phát triển 1.2.4.1 Protein Protein thành phần quan trọng dùng để xây dựng tổ chức sản xuất enzyme cho thể Protein...
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Thử nghiệm sử dụng protein bột nhân hạt cao su thay thế một phần protein bột cá trong khẩu phần ăn của cá rô phi ( OROCHROMIS NILOTICUS ) giai đoạn thương phẩm nuôi trong giai
... nh protein, lipid, lợng, vitamin khoáng Nhu cầu thay đổi tuỳ thuộc loài cá giai đoạn phát triển 1.2.4.1 Protein Protein thành phần quan trọng dùng để xây dựng tổ chức sản xuất enzyme cho thể Protein ... sai khác so với công thức Chứng tỏ việc thay protein bột cá protein hạt cao su cho hiệu tốt Nh dùng protein bột nhân hạt cao su thay 5%, 10%, 15% protein bột cá phần ăn cá Rô phi vằn (Oreochromis ... khác (Vũ Duy Giảng, 2003) Cá Rô phi nhu cầu protein cố định song đòi hỏi phối hợp 20 axit amin thiết yếu không thiết yếu để tạo nên protein Tiêu hoá protein xảy trớc hết dới tác dụng enzyme, thức...
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Tài liệu How To Use the Six Laws of Persuasion during a Negotiation pptx
... authorities to sell their wares, “Experts say our product is the best.“ But who are these experts? What are their qualifications to make these claims? Do they have a vested interest in selling the company’s ... give people what they want via the Six Laws of Persuasion, they’ll most likely return the favor And when you recognize that you are being manipulated, you can call the other side on their tactics ... tactics that use the preceding Laws of Persuasion So how you successfully negotiate around these ploys? First, you can discuss the rules of the game When you recognize that the other side is using...
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Tài liệu How To Use the Six Laws of Persuasion during a Negotiation docx
... authorities to sell their wares, “Experts say our product is the best.“ But who are these experts? What are their qualifications to make these claims? Do they have a vested interest in selling the company’s ... give people what they want via the Six Laws of Persuasion, they’ll most likely return the favor And when you recognize that you are being manipulated, you can call the other side on their tactics ... tactics that use the preceding Laws of Persuasion So how you successfully negotiate around these ploys? First, you can discuss the rules of the game When you recognize that the other side is using...
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Tài liệu Module 4: Minimizing the Impact on Network Operations During an Upgrade doc
... it as the secondary DNS server for the existing zone After the zone transfer has taken place, reverse the roles so that the Windows 2000 DNS server is the primary DNS server for the zone The zone ... 1HWZRUN#6HUYLFHV#8VHG#IRU#$XWKHQWLFDWLRQ# The key to optimizing the network for Windows 2000 authentication is to understand what network services are used during the authentication process By locating these services on the network ... server is the primary DNS server for the zone You can then configure the zone to allow dynamic updates Lastly, prior to the upgrade of the first domain controller (the PDC), you can add the SRV...
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Tài liệu Module 7: Minimizing the Impact on Network Operations During a Domain Restructure docx
... under the security context of the user to be migrated b Store the exported keys securely during migration c Use CryptoAPI to re-import the exported keys into the migrated users PStore under the ... be moved to the target Windows 2000 domain during the restructuring process (QVXULQJ#WKDW#'+&3#6HUYLFHV#&RQWLQXH#WR#2SHUDWH# One of the goals in migrating the source DHCP services to the target ... $OORZLQJ#18//#6HVVLRQ#$XWKHQWLFDWLRQ# When users use their cloned accounts in the target domain, they authenticate against Active Directory rather than the Windows NT 4.0 Security Accounts Manager (SAM)...
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