bond forming reactions to the coupling of n protected peptides is dictated by the requirement to avoid epimerization 5 4h oxazolones from activated peptides
Báo cáo khoa học: In vitro gamma-secretase cleavage of the Alzheimer’s amyloid precursor protein correlates to a subset of presenilin complexes and is inhibited by zinc potx
Ngày tải lên :
16/03/2014, 23:20
... or the c-secretase inhibitor L-6 85, 458 at the concentrations indicated The reactions were analysed by anti-FLAG Western blot Note that activity was abolished in a dose-dependent manner upon addition ... Zinc binding to Ab has been shown to promote Ab oligomerization [22– 25] Since a functioning zinc-binding domain may be present in the Ab sequence of C99, we hypothesized that zinc may affect the ... increasing zinc content The quantitated data is shown in graphical form under each panel strategy for the prevention of AD An initial step in discovering c-secretase inhibitors is the development of assays...
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Báo cáo khoa học: The expression of retinoblastoma and Sp1 is increased by low concentrations of cyclin-dependent kinase inhibitors ppt
Ngày tải lên :
16/03/2014, 23:20
... minimizes nonspecific annealing of primers to nontarget DNA The lower solution contained the cDNA, the MgCl2, the dNTPs, the [32P]dATP[aP] and one half of the buffer, and the upper solution contained ... by transfecting a dhfr minigene in the presence and absence of CDK inhibitors When using p21, the expression vector corresponding to this protein was co-transfected together with the dhfr minigene ... dilution of the C-21 antibody against Oct-1 to normalize the results Quantification of the signal is shown in the bottom panel Rb and Sp1 protein levels were determined from the ratio of the intensities...
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Báo cáo khoa học: Deadenylation of interferon-b mRNA is mediated by both the AU-rich element in the 3¢-untranslated region and an instability sequence in the coding region pot
Ngày tải lên :
17/03/2014, 10:20
... determinant), has been mapped between nucleotides 51 3 54 5, the first nucleotide corresponding to the adenosine of the initiation codon Deletion of this element by itself from the IFN-b gene (pIFNCRIDHA) ... 1A) We then analysed, by Northern blot, the induction and decay of IFN-b transcript in the same conditions As shown in Fig 1B, IFN-b mRNA was detectable h after the beginning of the infection, reached ... region corresponding to the ARE (from nucleotides 740–8 15 of the mRNA) The pIFNCRIDHA and pIFNCRIDHAAU– constructs were obtained by deletion of a 32-nucleotide region (from 51 3 54 5) in the PIFNHA...
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Báo cáo khoa học: The activity of Plasmodium falciparum arginase is mediated by a novel inter-monomer salt-bridge between Glu295–Arg404 doc
Ngày tải lên :
29/03/2014, 23:20
... of these (Fig 6) In the NP ensemble, two interactions are broken between chain A and B, and chain B and C by the end of 20 ns The third interaction was transiently broken (chain C and A) In the ... and constant pressure (NP) and constant number of atoms, constant pressure and temperature (NPT) ensembles and persisted up to 50 ns in the NPT simulations The effect of removing the metal on ... of Mg2+ in the NP ensemble In the NPT ensembles, the interactions remain intact with and without Mg2+ but there is an increase in the average standard deviation of the salt-bridge distance in...
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Chapter 057. Photosensitivity and Other Reactions to Light (Part 5) pps
Ngày tải lên :
07/07/2014, 01:20
... within these portions of the solar spectrum Treatment of this PLE includes the use of sunscreens and the induction of hardening by the cautious administration of artificial UV-B (broad-band or narrow-band) ... The diagnosis can be confirmed by skin biopsy and by performing phototest procedures in which skin is exposed to multiple erythema doses of UVA and UV-B The action spectrum for PLE is usually ... narrow-band) and/or UV-A radiation for 2–3 weeks prior to initial sun exposure Phototoxicity and Photoallergy These photosensitivity disorders are related to the topical or systemic administration of...
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Báo cáo y học: "TNF inhibits production of stromal cell-derived factor 1 by bone stromal cells and increases osteoclast precursor mobilization from bone marrow to peripheral blood" ppt
Ngày tải lên :
09/08/2014, 10:23
... SDF-1 concentration in the bone marrow is maintained in the presence of TNF Since rheumatoid arthritis and other forms of inflammatory bone disorders are chronic diseases, however, multiple factors ... Xiaoyun Zhang for technical assistance with the histology, and Yan Lu for assistance with osteoclast bone resorption assay The present work is supported by research grants from the National Institute ... determined as in Figure Data are representative of two independent experiments represents a unique pathologic situation in chronic inflammatory arthritis, in that the entire process of generation of...
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Tài liệu Báo cáo khoa học: Autolytic activity of human calpain 7 is enhanced by ESCRT-III-related protein IST1 through MIT–MIM interaction pptx
Ngày tải lên :
18/02/2014, 04:20
... reaction occur in the autolysis Therefore, it is premature to draw conclusions regarding the mechanism of the enhancing effects of IST1 on mGFP–calpain 7–Strep autolysis in vitro Both mGFP–calpain ... amplified cDNA fragment was first inserted into the Zero Blunt TOPO PCR Cloning vector (Invitrogen), and the BamHI–EcoRI fragment was then inserted into the BamHI–EcoRI site of pGEX4T-2 The mutant of pGST– ... cocrystallization and X-ray analysis of tandem MIT domains of calpain and IST1 MIM elements, should clarify these issues in the future Although the physiological role of human calpain is still unknown, the...
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Báo cáo khoa học: Expression of heme oxygenase-1 is repressed by interferon-c and induced by hypoxia in human retinal pigment epithelial cells pot
Ngày tải lên :
23/03/2014, 13:20
... in immunodeficient mice, including the enhanced growth, angiogenesis and lung metastasis [53 ], and treatment of these mice with a HO-1 inhibitor reduced the occurrence of lung metastasis [53 ] These ... Effects of cytokines on HO-1 mRNA expression in human RPE cells Northern blot analyses of HO-1 mRNA and Bach1 mRNA in human RPE cells treated with the following cytokines: interferon-c (IFNc), TNF-a ... actinomycin D, an inhibitor of transcription, and cycloheximide, an inhibitor of translation, on the hypoxia-mediated induction of HO-1 mRNA in D407 cells (Fig 4) The expression levels of HO-1 and...
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Báo cáo y học: "Expression pattern analysis of transcribed HERV sequences is complicated by ex vivo recombination" ppsx
Ngày tải lên :
13/08/2014, 05:22
... recombination between the LTR's of a provirus, leaving one LTR behind The source of evolutionarily young and reasonably intact HML-2 sequences is hitherto unknown Based on HML-2 sequence analysis, ... breakpoint location in the full alignment, but did not assign parental sequences correctly (data not shown) In summary, the size of the dataset and the number of gap regions made the analysis very ... chromosomes and 11 Recombination events were predicted to have occured between nt 4 15 and 422, and between nt 58 3 and 651 , resulting in an explanation with zero nt differences Finally, HERV-KX sequence...
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THE DIMENSIONS OF AESTHETIC REACTIONS TO MUSIC doc
Ngày tải lên :
16/03/2014, 18:20
... experimental data Development of the Content Analysis Musicians and aestheticians have made various distinctions between types of musical response, and the present system draws on some of these ... musical meaning is embodied in the structure of the com position itself (congeneric), as distinct from being designative of other non-musical events (extrageneric) Wright goes on to delineate four ... This, too, has parallels, in the extensive literature on concept formation (see e.g Kagan and Kogan, 1970) Our content analysis scheme represents an attempt to operationalise these distinctions in...
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A Perception-Based View of the Employee: A Study of Employees’ Reactions to Change doc
Ngày tải lên :
23/03/2014, 04:21
... dissertation is strictly limited to the examination of the relationships between perceptions and attitudes on the one hand and reactions to change on the other hand at the given moment in time rather ... perception of change in organizations In particular, this dissertation aims to contribute in this area by examining the link between various perceptions and attitudes on the one hand and resistance to ... can change the ways organizations work in response to changes in their operating environments is one of the main questions in the organization theory research There are two contrasting views: The...
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Báo cáo khoa học: A hydrophilic cation-binding protein of Arabidopsis thaliana, AtPCaP1, is localized to plasma membrane via N-myristoylation and interacts with calmodulin and the phosphatidylinositol phosphates PtdIns(3,4,5)P3 and PtdIns(3,5)P2 pptx
Ngày tải lên :
23/03/2014, 07:20
... localization and physiological functions can be regulated in cells N- myristoylation is the covalent attachment of a myristoyl group via an amide bond to the N- terminal Gly residue of a nascent polypeptide ... cation-binding myristoylated protein as a donor of the signaling compound, inositol 1,4,5phosphate [9] Unfortunately, information concerning PtdIns(3 ,5) P2 in plants is limited Recently, hyperosmotic and ... PtdIns(3,4 ,5) P3 in the membrane N- myristoylation anchors PCaP1, and the interaction with PtdInsPs may contribute to the stability of the attachment of PCaP1 to the membrane It has been shown that the...
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báo cáo khoa học: "Hypersensitivity reactions to anticancer agents: Data mining of the public version of the FDA adverse event reporting system, AERS" ppsx
Ngày tải lên :
10/08/2014, 10:21
... bound of the 95% two-sided confidence interval (CI) exceeds [9] Signal detection using the IC is done using the IC0 25 metric, a criterion indicating the lower bound of the 95% two-sided CI of the ... Diagnosis and management of hypersensitivity reactions related to common cancer chemotherapy agents Ann Allergy Asthma Immunol 2009, 102:179-187 Lenz HJ: Management and preparedness for infusion ... was not large enough to detect signals The database and the data mining methods used herein are useful, but the number of co-occurrences is an important factor in signal detection Acknowledgements...
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Tài liệu Using Stored Procedures to Add, Modify, and Remove Rows from the Database phần 1 pdf
Ngày tải lên :
14/12/2013, 13:15
... @OldProductID AND ProductName = @OldProductName AND UnitPrice = @OldUnitPrice use SET NOCOUNT ON to suppress the return of the number of rows affected by the INSERT statement SET NOCOUNT ON add ... statement is returned, and the DataAdapter therefore gets the correct value Transact-SQL also has a SET NOCOUNT ON command to turn on the number of rows affected You can use a combination of SET NOCOUNT ... @OldUnitPrice Using SET NOCOUNT ON in Stored Procedures In Chapter 4, "Introduction to Transact-SQL Programming," you saw that you use the SET NOCOUNT ON command to prevent Transact-SQL from...
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Tài liệu Using Stored Procedures to Add, Modify, and Remove Rows from the Database phần 2 doc
Ngày tải lên :
14/12/2013, 13:15
... DataColumn is set in step This is because the ProductID is automatically generated by the database when the new row is pushed to the database by the Update() method in step When the Update() method is ... You can then read the new ProductID using myNewDataRow["ProductID"], which now contains the new ProductID This ProductID is then returned at the end of the AddDataRow() method The output from ... called, the AddProduct4() stored procedure is run to add the new row to the Products table The database then generates a new ProductID for the row, which is then returned by the AddProduct4() stored...
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Tài liệu The Go Big Now Guide - 5 Steps to Make the Law of Attraction Key Work for You docx
Ngày tải lên :
24/12/2013, 13:15
... Attraction is always working, whether you want to admit it or not The question isn’t IF the Law of Attraction is working The REAL question is if the Law of Attraction Key is working for you or against ... Attraction is bringing you RIGHT NOW then I’m not doing my job The point is you have already harnessed the Law of Attraction It is working EVERY MOMENT of every day The only problem is that, right now, ... Attraction Key is Inspired Action! The Law of Attraction doesn’t mean you sit around your house visualizing what you want and then wait for it to knock on your door That is the biggest misconception...
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Tài liệu Bond Market Structure in the Presence of Marked Point Processes doc
Ngày tải lên :
16/02/2014, 02:20
... contains only instantaneously a finite set of bonds (“basic bonds” at t) but when t varies, then the union of these sets of securities may happen to be infinite and even non-countable, and hence ... subdeterminants of the last n 1 columns and hence (by the induction hypothesis) nonzero Thus we see from (67) that det B(t) is an analytic function and, because of the assumed linear independence, it is ... orthogonal, by construction, to all X n If (ii) does not hold then X is nontrivial; this leads to an apparent contradiction 27 By experience from the theory of financial markets with finitely many...
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Tài liệu Banks’ exposure to interest rate risk, their earnings from term transformation, and the dynamics of the term structure pptx
Ngày tải lên :
16/02/2014, 06:20
... Germany investigate the determinants of the banks’ exposure to interest rate risk They find that the belonging to certain banking groups, the banks’ size, their earnings and balance composition, and ... banks) we not find any significant coefficient One possible explanation of this finding is that banks take the interest rate risk into account when they allocate the budgets to the different sorts of ... correlation between the earnings from term transformation and the risk premia on loans In times of a boom the term structure tends to be steep and the risk premia (and thereby the mark-up) tend to be...
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Tài liệu Báo cáo khoa học: Reversible tetramerization of human TK1 to the high catalytic efficient form is induced by pyrophosphate, in addition to tripolyphosphates, or high enzyme concentration ppt
Ngày tải lên :
18/02/2014, 12:20
... on the kinetics apparently depends on the enzyme concentration in a linear manner, and no transition to the catalytically highly active form was observed at concentrations of hTK1 below 10 ngÆmL)1 ... Km is the Michaelis constant and K0 .5 is the substrate concentration where v = 0 .5 Vmax When n = 1, there is no co-operativity, and K0 .5 = Km Enzymatic regulation of human TK1 Acknowledgements ... concentration on the oligomerization status, the elution profile of rhTK1 was analyzed under the conditions and at the concentrations outlined in Fig In Fig 5A, rhTK1 was applied at a concentration...
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