blending with the a and b pdf

Báo cáo khoa học: Expressed as the sole Hsp90 of yeast, the a and b isoforms of human Hsp90 differ with regard to their capacities for activation of certain client proteins, whereas only Hsp90b generates sensitivity to the Hsp90 inhibitor radicicol pdf

Ngày tải lên : 23/03/2014, 07:20

... PP30[hHsp9 0b] Ten micrograms of total soluble protein was gel fractionated, and then western blotted; the blot was then probed with anti-(Achlya Hsp90) monoclonal serum The bands indicated by an asterisk ... GCTTGAAGCAAGCCTCGAT GCCTGAGGAAGTGCACCATGGA, reverse primer CA GTAGCTTCATCTTTCGATCGACTTCTTCCATGCGA GA) The second PCR used a universal pair of primers [34,48] PJ69- 4a [48] was then transformed with ... signal-regulated kinase-5 (ERK5) mitogen-activated protein (MAP) kinase [18] GR assays indicated that human Hsp9 0a and Hsp9 0b, as well as the native yeast Hsp90s, were all capable of activating GR in these...

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Báo cáo khoa học: Coordination of three and four Cu(I) to the a- and b-domain of vertebrate Zn-metallothionein-1, respectively, induces signiﬁcant structural changes doc

Ngày tải lên : 07/03/2014, 09:20

... form a distorted chair The C-terminal a- domain is characterized by an adamantane-like four-metal cluster Solution structures of 113 Cd-substituted Cd7MT-2 from rabbit, rat and human are available ... synthesis was from Rapp Polymere (Tubingen, Germany), and all ¨ other chemicals were from Merck (Darmstadt, Germany) Synthesis of the individual a- and b- domains The individual a- and b- domains ... still the possibility that the separated domains are simply incapable of binding more than three and four Cu(I) without aggregating and denaturing, whereas in the native MT-1, the presence of the...

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Báo cáo khoa học: Mutual effects of proton and sodium chloride on oxygenation of liganded human hemoglobin Oxygen afﬁnities of the a and b subunits potx

Ngày tải lên : 16/03/2014, 14:20

... afﬁnity, K, can be derived for both the a and b subunits from the averaged parameters of HbA oxygenation (Table 1, Average) The association and dissociation rate constants for the b subunits are found ... ligands leaves the a subunits (Table 1, ), and in every six ligands leaves the b subunits (Table 1, ) Using Eqns (3) and (4), the dissociation rate constant, k, and the O2 afﬁnity, K, can ... Here k a and k b are, respectively, the rate constants of BR for the a and b subunits within triliganded HbA Time courses for O2 rebinding are shown in Figs and The transient absorption decays were...

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The a and b adapters are used as priming sites for both amplification

Ngày tải lên : 19/03/2014, 22:32

... Preparation of the DNA • DNA is fragmented by nebulization • The DNA strand’s ends are made blunt with appropriate enzymes • A and B adapters are ligated to the blunt ends using DNA ligase ... filters all DNA rich beads from empty beads, and then extracts the biotin beads from the DNA rich beads • The DNA in the beads are denatured again using sodium hydroxide, creating ssDNA rich beads ... since their composition is known • The B adapter contains a 5’ biotin tag used for mobilization • The beads are magnetized and attract the biotin in the B adaptors Filtering the Mess • There are...

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Báo cáo khoa học: Cloning of a rat gene encoding the histo-blood group A enzyme Tissue expression of the gene and of the A and B antigens potx

Ngày tải lên : 31/03/2014, 09:20

... for the phylogenetic analysis Enzyme Species GenBank/EBI A transferase A transferase A transferase A (cis A/ B) transferase A- likea Gal transferase Gal transferase Gal transferase Gal transferase ... members, the ABO gene itself, the a3 galactosyltransferase (pseudo B) gene [17], the aN-acetylgalactosaminyltransferase or Forssman synthetase gene [18] and the a- galactosyltransferase iGb3 synthetase ... Ó FEBS 2002 kidney, the urinary bladder, the uterus and the thymus A weaker signal was obtained from the pancreas and very weak, barely detectable, signals were visible from a salivary gland,...

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Báo cáo hóa học: " Cloning of the canine RNA polymerase I promoter and establishment of reverse genetics for influenza A and B in MDCK cells" pdf

Ngày tải lên : 20/06/2014, 01:20

... Rat TATTGGACCTGGAGATAGGTACTGACACGC Mouse TTTGGGCCGCCGGGTTATATGCTGACACGC 216 bases TGCCTTATATGTTCGTCTGTAGGAGCGAGT GCATGTGCGGGCAGGAAGGTAGGGGAAGAC GATC Drosophila TATTGTACCTGGAGATATATGCTGACACGC 726,713 ... 90 pI tI BbsI XhoI BbsI CG ACCTCCG TTG G G G AG AAG G G G AG TCTTCTCG TAG ACCG AG AAG ACCTACCTG CAACAAAAAATG G T G G G CTG AG CTTCAACCCCCCCTCTCAG AG AAG CTCATCTTCTG CTG ATG ACCG TTTTTTACA G G G ... TGTTTCGGCGACAGGCAGACAGACGACAGGCAGACGTAAAAGACAGCCGGTCCGTCCGTCGCTCGCCTTAGAGATGTGGGCCTCTGGGCGCGGGTGGGGTTCCGGGCTTGACCGCGCGGCCGAGCCGG Consensus (1851) TGTTTCGGCGACAGGCAGACAGACGACAGGCAGACGTAAAAGACAGCCGGTCCGTCCGTCGCTCGCCTTAGAGATGTGGGCCTCTGGGCGCGGGTGGGGTTCCGGGCTTGACCGCGCGGCCGAGCCGG...

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Báo cáo hóa học: " Research Article A Hilbert-Type Linear Operator with the Norm and Its Applications" pdf

Ngày tải lên : 22/06/2014, 02:20

... theoretical analysis and applications These inequalities and their integral forms have been recently extended or strengthened in 4–8 Zhao and Debnath obtained a Hilbert-Pachpatte’s reverse inequality ... Mathematics and Its Applications (East European Series), Kluwer Academic Publishers, Dordrecht, The Netherlands, 1991 M Gao and B Yang, “On the extended Hilbert’s inequality,” Proceedings of the ... proved that 1.7 and 1.8 are two equivalent inequalities and their constant factors π/sin π/p and π/sin π/p 2p are the best possible When α 1, the expressions 1.7 and 1.8 can be reduced to 1.3 and...

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Báo cáo khoa học: " Modeling effect of the septic condition and trauma on C-reactive protein levels in children with sepsis: a retrospective study" pdf

Ngày tải lên : 13/08/2014, 03:21

... septic category with diagnosis as predictors (model B) both reduces the variability of baseline CRP values and their rates of change by about 20% and 1.5%, respectively Because the variation remains ... groups are quite small We had to deal with the data we had available There were no more patients in the most severe category (fortunately for the patients) The estimates, however, can be biased by ... (which is actually model A) and DG, and based on these analyses we could draw the same conclusions concerning SEP and DG as we already had done Since the analysis was intended as exploratory, we...

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Tài liệu Global Warming A Mind Mapper''''s Guide to The Science and Solutions pdf

Ngày tải lên : 17/12/2013, 02:15

... natural as well as manmade part of the - Scientist at NASA, Dr James Hansen earth’s atmosphere which have the ability to trap and retain heat) in the atmosphere and re-emitted back to the earth ... ABOUT JANE GENOVESE Jane Genovese is a public speaker, university graduate of Law and Arts (majoring in Psychology) and passionate global warming advocate She became concerned about global warming ... such a disturbing celebrities and gossip, accumulating more and more place, acting like zombies in the material wealth and the next holiday overseas than face of global catastrophe? with the survival...

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Tài liệu Essay Writing for a Score of 6.0 on the TOEFL and TWE pdf

Ngày tải lên : 23/01/2014, 06:20

... learn about responsibility, - they can learn the value of money, - they can learn how to work as a member of a team B Body of the Essay Now you expand on the reasons you gave in the thesis statement: ... Good Body Paragraphs The essay will also be judged on the use of language Naturally the readers will notice grammatical errors and the number of errors in a paper They judge whether the errors make ... to eat out will be the main ideas for the body of the essay Main Ideas for the Body of the Essay Now we will develop these ideas into the three main idea paragraphs in the body of the essay We...

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Tài liệu Báo cáo khoa học: The chitinolytic system of Lactococcus lactis ssp. lactis comprises a nonprocessive chitinase and a chitin-binding protein that promotes the degradation of a- and b-chitin doc

Ngày tải lên : 18/02/2014, 08:20

... FEBS G Vaaje-Kolstad et al Degradation of a- and b- chitin The degradation rates of a- and b- chitin were assayed with LlChi1 8A in the presence or absence of LlCBP3 3A As both chitin variants, and ... machineries, such as in the lactic acid bacterium (LAB) Lactococcus lactis ssp lactis IL1403 LABs are Gram-positive, facultatively, anaerobic, fermentative bacteria that are of major importance in the food ... to as LlChi1 8A) ; a secreted family 33 CBP (yucG; protein referred to as LlCBP3 3A) ; and a family 20 N-acetylhexosaminidase (LnbA) The chiA and yucG genes are separated by 19 bp in an operon starting...

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Báo cáo Y học: Substrate selectivity and sensitivity to inhibition by FK506 and cyclosporin A of calcineurin heterodimers composed of the a or b catalytic subunit potx

Ngày tải lên : 24/03/2014, 03:21

... recombinant CnAa and CnAb baculoviruses (Fig 1B, C) Kinetic assays of CaNa and CaNb phosphatase activity Fig SDS/PAGE and Western blot analysis of baculovirus expressed CaN composed of CnAa or CnAb ... findings that the CnAa and CnAb catalytic subunits confer differences in substrate affinity and phosphatase activity (as shown by Table Summary of kinetic parameters of CaNa and CaNb phosphatase activities ... sequences of the CnAa and CnAb catalytic, CaM-binding, and CnBbinding domains, it was surprising to find differences in the phosphatase activities between CaNa and CaNb toward the same substrate The results...

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Behind with the Laundry and Living off Chocolate pdf

Ngày tải lên : 28/03/2014, 19:20

... to say the least She was completely besotted with the gorgeous Mark but hadn’t the courage to anything about it Every day, Laura was faced with a barrage of what Mark said or didn’t say, what ... people there, all laughing, chatting and trying to talk to each other above the music blaring out You can just about see someone at the other end of the room between the heads 30 Behind with the Laundry:Behind ... that I’d love to be able to say I had achieved, and at the very top of the list was my wish to become a professional dancer It was a long time ago now but dancing was in my blood I was passionate...

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Providing Clinicians with the Skills and Tools to Assess, Prevent and Treat Pediatric Obesity pdf

Ngày tải lên : 28/03/2014, 21:20

... theoretical rationale and art of constructive and culturally sensitive weight counseling for behavioral change The HOPE curriculum is web-based and is available to both future and current clinicians across ... and To know the specific eating and physical activity behaviors that promote maintenance of healthy weight BEHAVIORAL COUNSELING FOR To become familiar with the stages of obesity management and ... AMERICAN ASIAN/PACIFIC ISLANDER BUILDING A SYSTEM FOR OBESITY MANAGEMENT ADVOCACY AND CHILDHOOD OBESITY The HOPE Project ADVOCACY AND CHILDHOOD OBESITY To be aware of social, economic, and environmental...

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Báo cáo khoa học: Identiﬁcation of the N-termini of NADPH : protochlorophyllide oxidoreductase A and B from barley etioplasts (Hordeum vulgare L.) ppt

Ngày tải lên : 30/03/2014, 02:20

... signiﬁcantly increased mass signals of sodium and potassium alkali metal and various di- and tri-alkali metal adducts if standard solvents with 0.1% formic acid were used for the UPLC separation ... mM ammonium formate One half of each sample was separated by UPLC after in-gel acetylation and digestion without further modiﬁcation (A) The second part of the sample was separated after further ... the plastid stroma after import and before the protein is assembled into an enzymatically active form As a number of groups have found that accumulation of PORA in the plastid stroma is a substrate-independent...

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Báo cáo khoa học: Characterization of the 5¢ untranslated region of a and b isoforms of the human thromboxane A2 receptor (TP) Differential promoter utilization by the TP isoforms doc

Ngày tải lên : 31/03/2014, 09:20

... 45 by up-regulating the synthesis and release of endogenous basic fibroblast growth factor J Biol Chem 268, 17397–17403 Lianos, E .A & Bresnahan, B .A (1999) Effect of thromboxane A2 inhibition and ... antagonism on prostaglandin and leukotriene synthesis in glomerular immune injury J Laboratory Clin Med 134, 478–482 Ushikubi, F., Aiba, Y., Nakamura, K., Namba, T., Hirata, M., Mazda, O., Katsura, ... the large distance, 1000 bp, between the first strand cDNA primer, Kin58 and the putative 5¢ terminus of the TPb mRNA transcript (data not shown) Thereafter, employing a second 5¢ RACE approach...

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Báo cáo sinh học: " Open Access Cloning of the canine RNA polymerase I promoter and establishment of reverse genetics for influenza A and B in MDCK cells" docx

Ngày tải lên : 18/06/2014, 18:20

... Rat TATTGGACCTGGAGATAGGTACTGACACGC Mouse TTTGGGCCGCCGGGTTATATGCTGACACGC 216 bases TGCCTTATATGTTCGTCTGTAGGAGCGAGT GCATGTGCGGGCAGGAAGGTAGGGGAAGAC GATC Drosophila TATTGTACCTGGAGATATATGCTGACACGC 726,713 ... 90 pI tI BbsI XhoI BbsI CG ACCTCCG TTG G G G AG AAG G G G AG TCTTCTCG TAG ACCG AG AAG ACCTACCTG CAACAAAAAATG G T G G G CTG AG CTTCAACCCCCCCTCTCAG AG AAG CTCATCTTCTG CTG ATG ACCG TTTTTTACA G G G ... TGTTTCGGCGACAGGCAGACAGACGACAGGCAGACGTAAAAGACAGCCGGTCCGTCCGTCGCTCGCCTTAGAGATGTGGGCCTCTGGGCGCGGGTGGGGTTCCGGGCTTGACCGCGCGGCCGAGCCGG Consensus (1851) TGTTTCGGCGACAGGCAGACAGACGACAGGCAGACGTAAAAGACAGCCGGTCCGTCCGTCGCTCGCCTTAGAGATGTGGGCCTCTGGGCGCGGGTGGGGTTCCGGGCTTGACCGCGCGGCCGAGCCGG...

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báo cáo hóa học:" Dynamical analysis of a biological resource management model with impulsive releasing and harvesting" pdf

Ngày tải lên : 21/06/2014, 17:20

... Dynamical analysis of a biological resource management model with impulsive releasing and harvesting Jianjun Jiao∗1 , Lansun Chen2 and Shaohong Cai1 School of Mathematics and Statistics, ... Introduction Biological resources are renewable resources Economic and biological aspects of renewable resources management have been considered by Clark [1] In recent years, the optimal management ... this article to analyze the exploitation of the predator–prey model with impulsive releasing and harvesting at diﬀerent moments Impulsive delay diﬀerential equations are suitable for the mathematical...

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2000 Test exam with A and B docx

Ngày tải lên : 26/07/2014, 10:20

... 53 They are tall a the b a c an d no article > d 54 Hoa is good pupil a the b a c an d no article > b 55 That is a bag It is on table a the b a c an d no article > a 56 We are in same ... Two things are very alike, so you say they are the each other a same with b same as c same like d same about > b 206 You like chocolate, a and I either b and me too c and I too d and so I ... d 290 Always make sure your luggage has on it when you travel a a card b a cartel c a label d a traveling-bag > c 291 Last December the boss gave all his a bonus a employ b employable c...

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