biến động chất lượng nước sông lô đoạn chảy qua thành phố tuyên quang từ 2009 2011

Plastid Biology, Advances in Plant Biology Volume 5

Ngày tải lên : 11/06/2015, 11:24

... Echeverria M, Forner J, Binder S, Marchfelder A (2009) Arabidopsis encodes four tRNase Z enzymes Plant Physiol 150:1494–1502 44 Castandet B, Araya A (2011) RNA editing in plant organelles Why make ... Small I (2011) Organellar RNA editing Wiley Interdiscip Rev RNA 2:493–506 52 Chateigner-Boutin AL, des Francs-Small CC, Delannoy E, Kahlau S, Tanz SK, de Longevialle AF, Fujii S, Small I (2011) ... genes are predominantly unspliced in leaf meristems and roots Plant Cell 1:437–445 14 Barkan A (2011) Expression of plastid genes: organelle-specific elaborations on a prokaryotic scaffold Plant...

585
1.3K
0

Báo cáo sinh học: "Respiratory syncytial virus infection in Fischer 344 rats is attenuated by short interfering RNA against the RSV-NS1 gene" doc

Ngày tải lên : 14/08/2014, 19:22

... a concentration of 0.02% and heated at 55°C for 10 The plasmid solution was then mixed with an equal amount of chitosan solution (5:1, weight to weight ratio of chitosan to DNA) and vortexed at ... group) were given a single intranasal dose of 200 µg of siNS1 or control plasmid complexed with an equal amount of chitosan After 24 h, they were intranasally inoculated with 1.4 × 107 PFU of RSV ... homogenized at 4°C using a Polytron (Brinkmann, Westbury, NY) Lung homogenates were centrifuged and equal amounts (based on the weight of lung homogenized) of supernatants were diluted 1:4 into EMEM...

8
218
0

Tài liệu Báo cáo khoa học: TMPRSS13, a type II transmembrane serine protease, is inhibited by hepatocyte growth factor activator inhibitor type 1 and activates pro-hepatocyte growth factor pdf

Ngày tải lên : 15/02/2014, 01:20

... fraction was treated overnight with enterokinase at unitsÆ100 lL)1 Quantiﬁcation of TMPRSS13 The enterokinase-treated pro-TMPRSS13 was quantiﬁed by immunoblotting using the protein expressed in E coli ... 185–193 Bugge TH, Antalis TM & Wu Q (2009) Type II transmembrane serine proteases J Biol Chem 284, 23177– 23181 Choi SY, Bertram S, Glowacka I, Park YW & Pohlmann S (2009) Type II transmembrane serine ... growth factor by proteolytic conversion of a single-chain form to a heterodimer J Biol Chem 267, 20114 20119 FEBS Journal 277 (2010) 4888–4900 ª 2010 The Authors Journal compilation ª 2010 FEBS 4899...

13
641
0

Báo cáo khoa học: Double-stranded RNA-dependent protein kinase (PKR) is downregulated by phorbol ester ppt

Ngày tải lên : 23/03/2014, 13:20

... separated by SDS ⁄ PAGE on 10% gels and subjected to autoradiography (B) PKR autophosphorylation was quantified by PhosphorImager analysis The results represent data from three separate experiments ... for 60 PKR autophosphorylation was determined as described above (B) PKR autophosphorylation was quantified by PhosphorImager analysis The results represent data from three separate experiments ... for 60 PKR autophosphorylation was analysed as described above (B) PKR autophosphorylation was quantified by a densitometry lane 5) or without (lane vs lane4) poly I:C is almost same (Fig 4) There...

9
168
0

Báo cáo y học: "Collagen type II (CII)-specific antibodies induce arthritis in the absence of T or B cells but the arthritis progression is enhanced by CII-reactive T cells" ppsx

Ngày tải lên : 09/08/2014, 01:24

... transfer of antibodies The cocktail of M2139 and C1 monoclonal antibodies was prepared by mixing equal concentrations of each of the sterile filtered antibody solutions to get a final amount of ... and interferon-γ 101 10–0 10–1 10–2 10–3 10–4 10–5 10–6 As a control, groups of mice received equal volumes of PBS On day 5, lipopolysaccharide (25 µg per mouse) was injected intraperitoneally...

7
434
1

Báo cáo y học: "Nociceptive tolerance is improved by bradykinin receptor B1 antagonism and joint morphology is protected by both endothelin type A and bradykinin receptor B1 antagonism in a surgical model of osteoarthritis" pdf

Ngày tải lên : 12/08/2014, 15:23

... decay equation: TE S(TE) = M0 exp − T2 where signal intensity S is defined as a function of echo time TE, and is related to the spin density M0 and the transverse relaxation time T The equation ... declare that they have no competing interests Received: 28 July 2010 Accepted: 16 May 2011 Published: 16 May 2011 Page 10 of 11 References Goldring MB, Goldring SR: Osteoarthritis J Cell Physiol ... limb as a percentage of total weight bearing on both hind limbs was calculated by the following equation [37]: % Weight on right leg = Weight on right leg × 100 Weight on right leg + Weight on...

11
378
0

KRONE - What is Patch by Exception

Ngày tải lên : 26/10/2013, 19:15

... patented point method of termination which outperforms the competition in ease of installation, quality, and durability of the connection • • • Insulation clamping ribs hold the wire firmly in...

3
449
0

Tài liệu Báo cáo khoa học: Membrane targeting and pore formation by the type III secretion system translocon pdf

Ngày tải lên : 14/02/2014, 22:20

... Microbiol 4, 811–825 ´ Galan JE (2009) Common themes in the design and function of bacterial effectors Cell Host Microbe 5, 571–579 Marlovits TC & Stebbins CE (2009) Type III secretion systems ... Katona G, Engstrom S & ¨ ¨ Neutze R (2009) Membrane protein crystallization from lipidic phases Curr Opin Struct Biol 19, 372–378 Bartesaghi A & Subramaniam S (2009) Membrane protein structure determination ... hydrophobic translocators of the Inv-Mxi-Spa system [51], proteins that display FEBS Journal 278 (2011) 414–426 ª 2010 The Authors Journal compilation ª 2010 FEBS 417 Membrane targeting and pore...

13
647
0

Tài liệu Báo cáo khoa học: Autolytic activity of human calpain 7 is enhanced by ESCRT-III-related protein IST1 through MIT–MIM interaction pptx

Ngày tải lên : 18/02/2014, 04:20

... shape of the ESCRT machinery Nat Rev Mol Cell Biol 8, 355–368 26 McDonald B & Martin-Serrano J (2009) No strings attached: the ESCRT machinery in viral budding and cytokinesis J Cell Sci 122, ... budding Dev Cell 15, 62–73 Bajorek M, Morita E, Skalicky JJ, Morham SG, Babst M & Sundquist WI (2009) Biochemical analyses of human IST1 and its function in cytokinesis Mol Biol Cell 20, 1360–1373 ... Agromayor M, Carlton JG, Phelan JP, Matthews DR, Carlin LM, Ameer-Beg S, Bowers K & Martin-Serrano J (2009) Essential role of hIST1 in cytokinesis Mol Biol Cell 20, 1374–1387 Rue SM, Mattei S, Saksena...

15
505
0

Tài liệu Báo cáo khoa học: High-afﬁnity ligand binding by wild-type/mutant heteromeric complexes of the mannose pptx

Ngày tải lên : 18/02/2014, 08:20

... preferential cross-bridging between wild-type receptors FEBS Journal 276 (2009) 1915–1929 ª 2009 The Authors Journal compilation ª 2009 FEBS Ligand binding by the dimeric M6P ⁄ IGF2R M A Hartman et ... ligand that should bind independently to each avail- FEBS Journal 276 (2009) 1915–1929 ª 2009 The Authors Journal compilation ª 2009 FEBS 1917 Ligand binding by the dimeric M6P ⁄ IGF2R 125I-PMP-BSA ... individually immunoprecipitated FLAG-tagged mini- FEBS Journal 276 (2009) 1915–1929 ª 2009 The Authors Journal compilation ª 2009 FEBS Ligand binding by the dimeric M6P ⁄ IGF2R M A Hartman et...

15
486
0

Tài liệu Báo cáo khoa học: Reversible tetramerization of human TK1 to the high catalytic efﬁcient form is induced by pyrophosphate, in addition to tripolyphosphates, or high enzyme concentration ppt

Ngày tải lên : 18/02/2014, 12:20

... data were ﬁtted by non-linear regression analysis to the Michaelis–Menten equation v ¼ Vmax Â ½S=ðKm þ ½SÞ or the Hill equation v ¼ Vmax Â ½Sn =ðK0:5n þ ½Sn Þ using prism from GraphPad Software ... absence of MgCl2, as the combination of MgCl2 and Chaps causes a heavy precipitate FEBS Journal 276 (2009) 571–580 ª 2008 The Author Journal compilation ª 2008 FEBS 573 Enzymatic regulation of human ... kinetics with low Km values similar to the nucleoside di- and tri-phosphates, and FEBS Journal 276 (2009) 571–580 ª 2008 The Author Journal compilation ª 2008 FEBS B Munch-Petersen Enzymatic regulation...

10
647
0

Tài liệu Báo cáo khoa học: Olfactory receptor signaling is regulated by the post-synaptic density 95, Drosophila discs large, zona-occludens 1 (PDZ) scaffold multi-PDZ domain protein 1 pptx

Ngày tải lên : 18/02/2014, 13:20

... in the olfactory epithelium and found MUPP1 to be FEBS Journal 276 (2009) 7279–7290 ª 2009 The Authors Journal compilation ª 2009 FEBS R Dooley et al PDZ proteins interact with olfactory receptors ... translated the PDZ domains of MUPP1, in pairs (1 + 2, FEBS Journal 276 (2009) 7279–7290 ª 2009 The Authors Journal compilation ª 2009 FEBS 7281 PDZ proteins interact with olfactory receptors R Dooley ... Table S1 A1 and A2 serve as negative controls 7282 FEBS Journal 276 (2009) 7279–7290 ª 2009 The Authors Journal compilation ª 2009 FEBS R Dooley et al PDZ proteins interact with olfactory receptors...

12
543
0

Tài liệu Báo cáo khoa học: Phosphorylation of cyclin dependent kinase 4 on tyrosine 17 is mediated by Src family kinases pptx

Ngày tải lên : 18/02/2014, 18:20

... nuclear lysate compared to HeLa whole cell lysate Due to the commercial availability of suitable quantities of HeLa nuclear pellets, these were chosen as the starting material for a puriﬁcation ... reduce the level of Y17 phosphorylation (data not shown); however, this result could be due to inadequate knockdown of C-YES or compensation by other Src family members It is known that Src family...

11
456
0

Tài liệu Báo cáo khoa học: Staphylococcal enterotoxin C1-induced pyrogenic cytokine production in human peripheral blood mononuclear cells is mediated by NADPH oxidase and nuclear factor-kappa B doc

Ngày tải lên : 19/02/2014, 00:20

... Dynatech, MeLean, VA, USA) 96-well plate reader at a wavelength of 450 nm Cytokine levels were quantiﬁed by comparison with standards The sensitivity of IL-1b and IL-6 was < 0.1 and < 0.7 pgÆmL)1, ... the cells treated with ngÆmL)1 of SEC1 for 24 h The NF-jB-DNA binding activity (lanes 2–8) was quantiﬁed by densitometry The time-course groups were compared with the Ctl group to obtain the ... necrosis factor (TNF-alpha) and lymphotoxin (TNF-beta) in the human genome Cold Spring Harb Symp Quant Biol 51 Part 1, 611–624 Spink J, Cohen J & Evans TJ (1995) The cytokine responsive vascular...

13
465
0

Tài liệu Báo cáo khoa học: Aggregative organization enhances the DNA end-joining process that is mediated by DNA-dependent protein kinase pdf

Ngày tải lên : 19/02/2014, 06:20

... residual molecules in the supernatant (Fig 1C, upper panel) The increasing amounts of extracts quantitatively precipitated the DNA, ending up with saturation at a full extent (lanes and 14) Considering ... and must be an open system Thus, we argue that this organization of nucleoproteins on linear DNA qualiﬁes as coacervation, which has been implicated in the mechanism for the condensed process of ... form of closed circular doublestrand DNA as an undamaged substrate limited the composition and quantity of aggregative proteins (lane 6) It should be noted that proteins that speciﬁcally aggregate...

13
458
0

Tài liệu Báo cáo khoa học: Selection of stably folded proteins by phage-display with proteolysis docx

Ngày tải lên : 19/02/2014, 12:20

6
444
0

Tài liệu Báo cáo khoa học: Hu-K4 is a ubiquitously expressed type 2 transmembrane protein associated with the endoplasmic reticulum ppt

Ngày tải lên : 19/02/2014, 17:20

... A 105 75 50 35 + - + + Weak Weak > 54 aa aa T C C T G Weak Weak Adequate Weak Adequate aa 58 aa 13 aa 33 aa 31 aa Adequate Adequate aa 24 aa ATG ATG ATG ATG ATG C B αHu-K4 + PIS Peptide - Peptide ... present, an adequate sequence comprises only one, a strong context both [13] Position ATG1 ATG2 ATG3 ATG4 Kozak consensus A GCC ATG G Context 330 345 396 489 AAG CTG CCC CTG Adequate Weak Weak ... codons are found in exons 1, 2, 3, 4¢ and 4, but only those in exons 3, 4¢ and are located in an adequate context for translational start (Table 2) Taken together, these data suggest that the smaller...

9
518
0

Tài liệu Báo cáo khoa học: Transcription of individual tRNA1Gly genes from within a multigene family is regulated by transcription factor TFIIIB pdf

Ngày tải lên : 20/02/2014, 02:21

... vitro transcription of these gene copies in PSG nuclear extracts is shown at the bottom and the quantiﬁed transcription levels as the percentage of tRNA1Gly -1 taken as 100, are indicated on the ... activity Evidently, the fractions were devoid of mutual contamination In every fractionation the quantities of fractions had to be optimized because use of larger amounts of any individual fraction ... the interaction of TFIIIB with tRNA1 -1 was Gly more stable than the interaction with tRNA1 -6,7 Quantiﬁcation of the ratio of heparin-resistant complexes to the TFIIIB ⁄ C–DNA complexes in the...

15
484
0

Tài liệu Báo cáo khoa học: Gene transcription of fgl2 in endothelial cells is controlled by Ets-1 and Oct-1 and requires the presence of both Sp1 and Sp3 pdf

Ngày tải lên : 20/02/2014, 11:20

... transcriptase was omitted (data not shown) RT-PCR for b-actin was set up as an internal control to ensure equal loading and ﬁrst strand synthesis with forward primer, 5¢-ATGTTTGAGACCTTCAACAC-3¢, and reverse ... (Eur J Biochem 270) 2279 Fig Functional analysis of fgl2 promoter (A) Analysis of fgl2 RNA by semiquantitative RT-PCR RT-PCR was performed with lg of each RNA sample M, macrophages; M + MHV-3, macrophages ... However, when both threshold amounts (10 ng) of Sp1 and Sp3 were added together, with increasing quantities of Ets-1, a cooperative positive functional interaction was evident (Fig 9B) To deﬁne...

13
525
0

Tài liệu Báo cáo Y học: Thermostability of manganese- and iron-superoxide dismutases from Escherichia coli is determined by the characteristic position of a glutamine residue pdf

Ngày tải lên : 21/02/2014, 01:21

... or in the presence of sodium azide, an initial dilution of SOD was adjusted to give Vs equal to half Vb (equal to unit of SOD activity under standard conditions) After incubation of aliquots at ... with cultures grown to exponential phase in the absence of paraquat and IPTG (Materials and methods [24]) The ﬁnal concentration of paraquat and IPTG used for the experiment presented were chosen ... Anal Biochem 44, 276–287 43 Bradford, M.M (1976) A rapid and sensitive method for quantitation of microgram quantities of protein utilising the principle of protein binding Anal Biochem 72, 248–254...

12
740
0