biotin streptavidin or tsa method

Standard Test Method for Compressive Strength of Hydraulic Cement Mortars (Using 2-in. or [50-mm] Cube Specimens)

... water-cement ratio of 0.485 for all portland cements and 0.460 for all air-entraining portland cements The amount of mixing water for other than portland and air-entraining portland cements shall be ... laboratory shall be not less than 50 % The moist closet or moist room shall conform to the requirements of Speciﬁcation C511 Test Specimens 8.1 Make two or three specimens from a batch of mortar ... portland and air-entraining portland cements, merely record the ﬂow 10.3.3 In the case of cements other than portland or airentraining portland cements, make trial mortars with varying percentages...

Báo cáo khoa học: "A PARSING METHOD OR INFLECTIONAL FREE WORD ORDER LAN" docx

Danh mục: Báo cáo khoa học

... description of what dependants a word can have and in what order v v V V V //////\ In a free Word order language we would f~ce, for e x i l e , a paradigm fragment of the form subj , obj , advl , obj ... standard notion to recognize left and right dependants of a word starting from its immediate neighbour Instead of recognizing words (or word categories) these automata recognize functions, i.e instances ... deletes non-matchina [mterore~ations of an ambigu(~]s word, or an actier which performs one of the basic construction operations such as labelling (:=), attaching (:-), or deletion, and returns...

Báo cáo y học: "Are chiropractors in the uk primary healthcare or primary contact practitioners?: a mixed methods study" docx

Danh mục: Báo cáo khoa học

... thanks to her supervisor, Professor J Bolton, for her support and advice throughout the Doctoral research process as well as her critical review of this manuscript Authors’ contributions AJH ... chiropractors usually provide a service that fulfils the requirements for coordination of care, and can readily achieve the requirements for first-contact by extending opening hours or providing ... medical and health care needs - not limited by problem origin, organ system, or diagnosis Primary care physicians are advocates for the patient in coordinating the use of the entire health care system...

thảo luận Tiếng anh VCU Give definition of marketing and explain why marketing is important. what things are included in marketing Suggest some best marketing methods or techniques.

Danh mục: Đại cương

... product, service or brand to customers, for the purpose of promoting or selling that product, service, or brand II Importance of marketing Page | Marketing is one of the more important components ... press releases, sponsorship deals, exhibitions, conferences, seminars or trade fairs and events Word-of-mouth is any Page | apparently informal communication about the product by ordinary individuals, ... customers or people specifically engaged to create word of mouth momentum Sales staff often plays an important role in word IV of mouth and public relations (see 'product' above) Popular marketing method...

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Give definition of marketing and explain why marketing is so important . What things are include in marketing ? Suggest some best marketing methods or techniques.

Danh mục: Mẫu Slide - Template

... costs III Main factors of marketing 1.Product (or Service) 2.Place 3.Price 4.Promotion IV/ Popular marketing method Social media marketing Social media marketing, or SMM, is a form of internet marketing ... Promotion Part Popular marketing method +Hooray for Free Advertising + The Bottom Line I.Marketing definition The activity of institutions The purpose of promoting or Delivering and exchanging ... request business, or solicit sales or donations Email marketing can be done to either sold lists or a current customer database A number of promotional tools The conference Event Workshop introduced...

Safe carrying methods

Danh mục: Kỹ năng quản lý

... responsible for safety issues These organisations include: Worksafe Western Australia South Australian Workcover Authority Northern Territory Work Health Authority Victorian Workcover Authority NSW Workcover ... Different Laws for different States or Territories Each State or Territory in Australia has its own workplace health and safety laws These laws are produced and regulated by State or Territory government ... Safety tips for working outdoors on hot, sunny days A good proportion of your daily work in aquaculture will be outdoors You may be out inspecting ponds, feeding stock, harvesting, or checking...

When the Discussion Gets Stalled or Heated

Danh mục: Kỹ năng quản lý

... Keep power in your pocket (e.g. attorney, credible reference) Keep the door open Conflict as a Choice Point  Explore whether conflict is a signal for a change  View the disagreement as a choice point and explore options for moving ... Pause when formulating judgment  Understand what was meant by actions or words  Reflect on information and ask for additional information  Reinterpret by applying an alternate explanation  Redirect conversation toward ... Dismissive – interrupting, talking over, ignoring {men engage more frequently than women} • Exclusionary – leaving someone out of the meeting, discussion or social gathering • Undermining – going behind the back of others or using insults to discredit arguments...

Quagmire or Gold Mine?

Danh mục: Kỹ thuật lập trình

... make digital/hard copy of part or all of this work for personal or classroom use is granted without fee provided that copies are not made or distributed for profit or commercial advantage, the ... the store’s responses In the software shopping domain, Shopbot was given the home pages for 12 online software vendors Shopbot learned to extract product information from each of the stores, ... in Information Retrieval (Copenhagen, Denmark), June 12, 1992, pp 318–329 Doorenbos, R.B Etzioni, O and Weld, D.S A scalable comparison-shopping agent for the world-wide web Technical Report 96–01–03,...

MOB Subject 5 –Access methods Static and dynamic access techniques

Danh mục: Kỹ thuật lập trình

...

Glycoprotein methods protocols - biotechnology 048-9-001-013.pdf

Danh mục: Sinh học

... In our laboratory, CsCl rather than CsBr or CsSO4, is used as the density gradient– forming salt since gradients are run in the presence of guanidinium chloride and the use of CsBr or CsSO4 in ... together and those that anchor cell-associated glycoproteins to the plasma membrane must be broken In our laboratory, high concentrations of guanidinium chloride are used for this purpose, and highshear ... guanidinium chloride, which considerably reduces the cost Ultrapure grade guanidinium chloride, which is much more expensive, may be used without prior purification Dissolve 765 g of guanidinium chloride...

Glycoprotein methods protocols - biotechnology 048-9-027-044.pdf

Danh mục: Sinh học

... retrieval may also be performed in a wet autoclave or pressure cooker (51,52) One should be aware that heat-based antigen retrieval may restore or induce endogenous biotin or biotin- like activity ... with biotinylated goat antimouse or rabbit immunoglobulins (Jackson ImmunoResearch, West Grove, PA) diluted 1:300–1:500 for 30 Wash in three changes of PBS for each Incubate sections with streptavidin- horseradish ... selection of particular fields must be valid For example, the invasive margin of a tumor may be more informative than an in situ component or areas of tumor necrosis 34 Walsh and Jass Assessment...

Glycoprotein methods protocols - biotechnology 048-9-045-055.pdf

Danh mục: Sinh học

... solution and /or nonionic detergent Block for 1–24 h at room temperature or 4°C 48 McGuckin and Thornton Wash three times for each in PBS Blocked plates can be used immediately; stored in PBS for several ... prepared for inclusion on each plate as a measure of interassay variation Thorough washing is important (e.g., three times for each in PBS-0.05% Tween-20; three times for each in PBS) More or less ... 3.2.3.) or store the membrane sealed in a bag in buffer at 4°C or dry thoroughly and store sealed at –20°C 3.2.2 Detection of Mucins on Membranes Using PAS (see Notes 10–12) Wash the dot-slot or Western...

Glycoprotein methods protocols - biotechnology 048-9-057-064.pdf

Danh mục: Sinh học

... and not contain any organic solvent (e.g., alcohol), which will cause dehydration and distortion of the mucous gel layer The unfixed section method is most satisfactory for gastric mucosa where ... results by this method with those of previous methods used for staining the mucous gel can be found in Jordan et al (10) An example of the use of this method for studies in humans is given in Newton ... concentration or a separate secretion This method, which for experimental practicalities can be applied only to the rat (and possibly other experimental animal models), is particularly useful for validating...

Glycoprotein methods protocols - biotechnology 048-9-065-073.pdf

Danh mục: Sinh học

... mucin-producing cell or tissue samples can be performed prior to or during the metabolic pulse labeling of the mucin-producing cell samples 10 In most cases [35S]sulfate is primarily incorporated into ... competes with the labeled mucin for the antibody (see also Chapter 20) Fortunately, it appears that the most prevalent mucin precursors in any particular organ or cell line can be distinguished ... mucin of interest for 90 b Wash two times for 15 each c Incubate with 0.5 µCi of (185 kBq) 125I-labeled protein A for 60 d Wash twice for each in Blotto, and then wash once for in phosphatebuffered...

Glycoprotein methods protocols - biotechnology 048-9-075-085.pdf

Danh mục: Sinh học

... (hand hot) and insert well-forming comb (see Note 2) Leave to set for at least h prior to use 3.3.2.3 ELECTROPHORESIS Electrophorese sample in electrophoresis buffer for 16 h at 30 V at room temperature ... reagent) and for lectin and antibody reactivity (see ref for detailed procedures) 3.3.2 Agarose Gel Electrophoresis Electrophoresis is performed in 1% (w/v) agarose gels using a standard horizontal ... subunits phoretic mobility observed with the salivary mucin sample is not normally seen in respiratory mucin subunit preparations In respiratory samples there are typically two gel-forming mucins,...

Glycoprotein methods protocols - biotechnology 048-9-087-096.pdf

Danh mục: Sinh học

... information is available from the rotor data sheet Using this information together with the data in Table 1, isokinetic gradients of guanidinium chloride can be designed for different rotors ... 6–8 M guanidinium chloride (see Note 4) as the gradient support medium, and mucins have been shown to have (for certain rotor geometries) isokinetic sedimentation behavior in this system (see ... of core proteins and glycoforms Biochem J 316, 967–975 Thornton, D J., Howard, M., Khan, N., and Sheehan, J K (1997) Identification of two glycoforms of the MUC5B mucin in human respiratory mucus:...

Glycoprotein methods protocols - biotechnology 048-9-111-119.pdf

Danh mục: Sinh học

... derivatized with 9-fluorenylmethyl chloroformate J Chromatogr 723, 219–225 Wilkins, M R., Yan, J X., and Gooley, A A (1999) 2-DE spots amino acid analysis with 9-fluorenylmethyl chloroformate, 2-D Protein ... are currently developing a method for quantitating cysteine by amino acid analysis Amino Acid Analysis of Mucins 119 Acknowledgments The authors acknowledge support for their research on amino ... and threonine Strong acid, high temperature, and short time acid hydrolysis (12 N HCl at 155°C for h) is generally used in our laboratory for less glycosylated proteins (1) We have found that...

Glycoprotein methods protocols - biotechnology 048-9-121-128.pdf

Danh mục: Sinh học

... can be electroblotted (see Note 6) or adsorbed onto Hyperbond or PVDF (see Note 7), or covalently coupled to an arylamine-derivatized solid support (see Note 8) For qualitative analysis of glycosylation ... have published alternative HPLC methods for the separation of PTH-glycosylated amino acids on a modified Beckman LF3600 sequenator (12) These methods were developed for two reasons: (1) to provide ... Hyperbond or PVDF Glycopeptides adsorbed onto PVDF or Hyperbond can be sequenced with conventional Edman degradation (Program 3.1 PVDF on the Hewlett Packard G1000A or the PVDF routine for the blot...

Glycoprotein methods protocols - biotechnology 048-9-129-141.pdf

Danh mục: Sinh học

... antigens for the interrogation of antibody specificity; however, the methodology for their preparation is beyond the scope of this chapter By far the most widely used method for modifying short peptides ... correct orientation and allow to incubate for 45 with agitation The reaction can be stopped before the time is elapsed, if it appears that the reaction will give an optical density (OD) of or ... epitope residues more closely resembles the native sequence RNET analysis offers the most critical and informative method for probing a peptide epitope in the MUC1 protein core In this approach,...

Glycoprotein methods protocols - biotechnology 048-9-143-155.pdf

Danh mục: Sinh học

... with 30 cycles at 94˚C for 30 s, 50˚C for and 72˚C for min, ending with a 72˚C extension for 148 Bell and Forstner Check the PCR product for size and purity by electrophoresis on a 1% agarose ... secretory mucin, MUC6 J Biol Chem 272, 16,398–16,403 19 Sambrook, J., Fritsch, E F., and Maniatis, T (1989) Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory, Cold Spring Harbor, ... Ismat Khatri DNA sequencing was performed by the Biotechnology Service Centre, The Hospital for Sick Children, Toronto, Canada References Forstner, J F and Forstner, G G (1994) Gastrointestinal...

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