biosynthesis of diacylglycerol in plants
Molecular Farming of Antibodies in Plants
... chains are shown in gray and light chains in pink Solid black lines indicate continuation of the polypeptide backbone (simple lines indicate the constant parts of the antibody, curly lines indicate ... expressed in plants Heavy-chain derivatives are shown in gray and light-chain derivatives in pink Solid black lines indicate continuation of the polypeptide backbone (simple lines indicate the ... Molecular Farming of Antibodies in Plants 37 rearrangements of the germ-line immunoglobulin genes This part of the molecule is responsible for antigen recognition and binding The remainder of the antibody...
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Báo cáo Y học: Stimulated biosynthesis of flavins in Photobacterium phosphoreum IFO 13896 and the presence of complete rib operons in two species of luminous bacteria Sabu Kasai and Takumi Sumimoto pot
... in basic amino acids P phosphoreum RibX was calculated to have a pI of 7.6 suggesting that the protein could be a DNA binding protein In B subtilis, regulation of the biosynthesis of riboflavin ... amount of flavins using the lumiflavin fluorescence method The term ÔflavinsÕ refers to riboflavin, FMN and FAD P-flavin, which is contained in the extract of luminous bacterial cells [12], is not included ... bioluminescence of the parent strain cultured in 3% NaCl, respectively To confirm that strongly light emitting cells produce large amounts of flavins, we determined the total amount of flavins in cells...
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Báo cáo y học: "Vive la différence: biogenesis and evolution of microRNAs in plants and animals" ppt
... heterogeneous small interfering RNAs (siRNAs) As regulatory relationships are refined, the precision and phasing of hairpin processing may increase Shortening of the hairpin to produce a single defined duplex ... in plants, but one short hairpin intron in rice (MIR1429) generates specific miRNA/miRNA* reads indicative of mirtron processing [58] In addition to other types of Drosha-independent miRNAs in ... controlled by independent regulatory elements [48-50] Unlike in plants, there are also occasional examples of miRNA biogenesis from exons of animal protein-coding genes, including UTRs and coding sequences...
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Tài liệu Báo cáo Y học: Studies on the nonmevalonate pathway of terpene biosynthesis The role of 2C-methyl-D -erythritol 2,4-cyclodiphosphate in plants docx
... corresponding scrambling within nonstarter C5-units derived from IPP in the elongation process has been observed in the mevalonate q FEBS 2001 independent biosynthesis of carotenoids in cell cultures of ... because of the inadequacy of the analytical tools employed in earlier work using a 14C label In all the cases in which such a scrambling was observed it was usually ascribed to a lack of fidelity of ... removal of one of its hydrogens (path b) The outcome of the two competing deprotonation paths is illustrated in Fig for the predominant ES complex A of a sample of IPP carrying a 13 C label in its...
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Regulating Phytonutrient Levels in Plants – Toward Modification of Plant Metabolism for Human Health
... others being delphinidin, peonidin, pelargonidin, petunidin, and malvidin Of the flavonols, quercetin, kaempferol, myricetin, and isorhamnetin are common in fruits, quercetin being the predominant ... 12 Regulating Phytonutrient Levels in Plants 311 12.5.4 Metabolic Engineering of the Flavonoid Biosynthetic Pathway in Tomato There is a growing interest in producing food plants with increased ... apigenin, luteolin), flavonols (e.g., quercetin, myricetin), flavanones (e.g., naringenin, hesperidin), catechins or flavanols (e.g., epicatechin, gallocatechin), anthocyanidins (e.g., cyanidin, pelargonidin),...
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Tài liệu Báo cáo khoa học: Crystal structure of thiamindiphosphate-dependent indolepyruvate decarboxylase from Enterobacter cloacae, an enzyme involved in the biosynthesis of the plant hormone indole-3-acetic acid doc
... presence of DNA-binding proteins involved in regulation of the gene expression of indole-3pyruvic acid decarboxylase, a key enzyme in indole-3-acetic acid biosynthesis in Azospirillum lipoferum FS Biosci ... responsible for binding and proper orientation of the aminopyrimidine ring, are conserved in all ThDP-dependent enzymes One of these, the hydrogen bond between the N1¢ atom of the pyrimidine ring of ThDP ... these interfaces is located in the PYR and middle domains The interface contains 10 hydrogen bonds in IPDC The dimer–dimer interface in IPDC is smaller than the corresponding interface in ˚ ZmPDC...
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Tài liệu Báo cáo Y học: Identification of a set of genes involved in the biosynthesis of the aminonucleoside moiety of antibiotic A201A from Streptomyces capreolus pdf
... implicated in the biosynthesis of the aminonucleoside moiety of A201A Here we report the sequencing of a total of 6946 bp, which include the ata genes most likely implicated in the biosynthesis of this ... transcription terminator The resulting fragment only contains the initial 67 bp and the final 68 bp of the pur4 coding sequence It was inserted in plasmid pGM9 The resulting construct was introduced, ... be implicated in the biosynthesis of the aminonucleoside moiety of puromycin [6–8] Interestingly, their organization is identical to that of the pur cluster, with the exception of pur6, which...
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Uptake of organic chemicals in plants Human exposure assessment ppt
... diffuse into the sampling phase depending on the type of fiber used After sampling, the fiber is injected into the inlet of a gas chromatograph for thermal desorption and determination of analytes ... modelling the uptake of organic compounds into plants any easier However, combining models and experiments gain a lot of insight into the topic, and models can be used to help design and interpret ... SPARC.8,9 Determining SPME Sampling Kinetics The SPME sampling kinetics were studied for a number of sample types in order to (1) determine the sampling time needed to ensure equilibrium sampling, (2)...
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Báo cáo khoa học: Arabidopsis thaliana CYP77A4 is the first cytochrome P450 able to catalyze the epoxidation of free fatty acids in plants potx
... monomer into the cutin In this context, it is interesting to note that inhibition studies allowed LeQueu et al [49] to demonstrate the involvement of a peroxygenase in the formation of cutin of corn ... acid in the absence (A) or presence (B) of NADPH Incubations were performed at 27 °C and contained 20 pmol of CYP77A4 They were stopped after 30 by the addition of 20 lL of acetonitrile (containing ... acid in the absence (A) or presence (B) of NADPH Incubations were performed at 27 °C and contained 20 pmol of CYP77A4 They were stopped after 30 by the addition of 20 lL of acetonitrile (containing...
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Báo cáo Y học: Molecular cloning and characterization of isomultiflorenol synthase, a new triterpene synthase from Luffa cylindrica, involved in biosynthesis of bryonolic acid ppt
... A., Inoue, K., Hiraoka, N., Ikeshiro, Y., Kushiro, T., Shibuya, M & Ebizuka, Y (2001) Cloning and characterization of a cDNA encoding b-amyrin synthase involved in glycyrrhizin and soyasaponin biosynthesis ... Differential expression of OSC mRNAs in the cultured cells of L cylindrica Figure shows the multiple alignments of deduced aminoacid sequences of three OSCs from L cylindrica The sequence of the Luffa LcIMS1 ... to the sites of pYES2 (Invitrogen), to obtain pYES2-LcIMS1 in which the ORF of the cDNA was ligated to the GAL1 promoter in the sense orientation The nucleotide sequence of the inserted DNA was...
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Báo cáo khoa học: Delineation of the roles of FadD22, FadD26 and FadD29 in the biosynthesis of phthiocerol dimycocerosates and related compounds in Mycobacterium tuberculosis pptx
... of the biosynthesis of DIMs and PGLs, the in vivo functions of these FadD proteins in this pathway remain to be established and ⁄ or completed For instance, the involvement of FadD26 in the biosynthesis ... FadD28 is involved in the formation of mycocerosic acids by activating the Mas substrates [14], whereas FadD26 was shown to be directly involved in DIM biosynthesis by catalyzing the loading of long-chain ... the production of DIMs in the PMM137:pM26D strain was lower than that in the wild-type strain (Fig 3B), suggesting partial restoration of DIM biosynthesis in the complemented strain This partial...
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Báo cáo khoa học: Improvement of ecdysone receptor gene switch for applications in plants: Locusta migratoria retinoid X receptor (LmRXR) mutagenesis and optimization of translation start site pptx
... fusion of GAL4 DNA-binding domain with CfEcR ligand-binding domain cloned under the control of the 35S promoter in the pKYLX80 vector A fusion of VP16 activation domain and LmRXR ligand-binding ... Interestingly, an increase in the ligandinduced activity of this switch containing the improved Kozak sequence was not accompanied by an increase in background activity in the absence of ligand ... addition of ligand, and the luciferase activity was measured and expressed in terms of RLU per microgram of protein In ligand withdrawal experiments, transgenic plants grown in soil containing 80...
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Báo cáo khoa học: Photosynthetic acclimation: State transitions and adjustment of photosystem stoichiometry – functional relationships between short-term and long-term light quality acclimation in plants ppt
... appearance of clouds and the leaf movement by wind Biotic in uences depend mainly on the individual position of a plant within an ecosystem, the density of the plant stand or, in case of algae, ... reduction in seed production, indicating synergistic effects when both kinases are lacking [9] Additional interesting results were obtained by some gene-expression studies The lack of LTR in the ... luminal side of the thylakoid membrane while the C-terminal kinase domain is on the stromal side [24] (Fig 2) Inactivation of these kinases therefore would require a mechanism by which reducing...
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Báo cáo khoa học: Improvement of a monopartite ecdysone receptor gene switch and demonstration of its utility in regulation of transgene expression in plants pdf
... construction of a versatile system for the expression of foreign genes in plants Gene 61, 1–11 Tavva VS, Collins GB & Dinkins RD (2006) Targeted overexpression of the Escherichia coli MinC protein in higher ... a DNAbinding domain (DBD) and a ligand-binding domain (LBD) The chimeric gene (transactivation domain– DBD–LBD) is expressed under the control of a constitutive promoter In the presence of a specific ... Arabidopsis plants were transformed using the whole plant-dip method [42] Transgenic Arabidopsis plants were selected by germinating the seeds collected from the in ltrated plants on a medium containing...
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Báo cáo khoa học: Geraniol dehydrogenase, the key enzyme in biosynthesis of the alarm pheromone, from the astigmatid mite Carpoglyphus lactis (Acari: Carpoglyphidae) docx
... compound in the monoterpene biosynthesis of mites Neral is one of the most widely distributed mite monoterpenes It is present in 35 of 60 species of astigmatid mites examined, including 28 unidentified ... lactis Linnaeus (Acari: Carpoglyphidae) was originally of a strain maintained at Tokyo Women’s Medical College The strain was maintained at 25 °C and 75% relative humidity, and fed a mixture of dry ... Amino acid sequences corresponding to several classes of ADH found in a wide range of species (animals, including insects, plants, and microorganisms) were obtained from UniProtKB ⁄ Swiss-Prot...
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Biochemical mechanisms of detoxification in higher plants
... most vitally important parameters determining the continuing existence of mankind The inexorable and seemingly permanent increase of technogenic contaminants in all ecological niches creates danger ... toxins, and guidelines defining acceptable toxin levels A number of medical supplies, including antibiotics, also belong to the category of substances of low toxicity In the remainder of this chapter, ... all polyvinyl chloride) Dioxins are also found in the gas used in the chlorination of water supplies Polychlorinated contaminants are formed as admixtures during the interaction of chlorine with...
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Báo cáo khoa học: Hidden stereospecificity in the biosynthesis of divinyl ether fatty acids ppt
... Stereospecificity of divinyl ether synthases [3,5] Further research stimulated by these findings has resulted in the cloning of genes encoding divinyl ether synthases in tomato and potato and in the identification ... observed in the biosynthesis of colneleic acid (Table 1, Fig 2) Stereospecificity of hydrogen eliminations from C8 in the biosynthesis of colneleic acid isomers Stereospecificity of hydrogen eliminations ... (1998) A pathway for biosynthesis of divinyl ether fatty acids in green leaves Lipids 33, 1061– 1071 13 Hamberg M (2002) Biosynthesis of new divinyl ether oxylipins in Ranunculus plants Lipids 37,...
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Báo cáo khoa học: Biosynthesis of platelet glycoprotein V expressed as a single subunit or in association with GPIb-IX doc
... selection in the absence of nucleosides and amplification of GPV expression by growing cells in the presence of step increased concentrations of methotrexate [21] This clone was maintained in suspension ... (1999) Glycoprotein (GP) Ib-IX-transfected cells roll on a von Willebrand factor matrix under flow Importance of the GPIb/actin-binding protein (ABP280) interaction in maintaining adhesion under ... Fox, J.E (1995) Interaction of platelet glycoprotein V with glycoprotein Ib-IX regulates expression of the glycoproteins and binding of von Willebrand factor to glycoprotein Ib-IX in transfected...
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Báo cáo khoa học: Biosynthesis of riboflavin in Archaea 6,7-Dimethyl-8-ribityllumazine synthase of Methanococcus jannaschii pdf
... Construction of an expression plasmid for modified lumazine synthase of Bacillus subtilis Fig Terminal reactions in the pathway of riboflavin biosynthesis The coding region of the ribH gene of B subtilis ... (1978) Presence in Escherichia coli of a deaminase and a reductase involved in biosynthesis of riboflavin J Bacteriol 136, 657–667 Hollander, I & Brown, G.M (1979) Biosynthesis of riboflavin: reductase ... (2002) Biosynthesis of riboflavin Single turnover kinetic analysis of 6,7dimethyl-8-ribityl-lumazine synthase J Am Chem Soc., in press 39 Laemmli, U.K (1970) Cleavage of structural proteins during...
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Báo cáo khoa học: The mitochondrial protein frataxin is essential for heme biosynthesis in plants potx
... exists on the role of this protein in the biogenesis of heme-containing proteins in plants To gain insight into this process, we decided to study the role of frataxin using the enzyme catalase as ... defects in the activity of several Fe–S-containing proteins, a reduction of heme a and concomitantly the cytochrome oxidase activity, suggesting an important role of frataxin in the biogenesis of ... demonstrated that frataxin serves as a potential donor to FC for insertion of iron into the protoporphyrin ring during heme synthesis Knocking down the expression of frataxin in human cells revealed...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn hệ số công suất cosp fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng 9 tr 25