biopsies in an ali culture model which will support experimental studies on hnespcs growth differentiation and biological morphology in an in vitro system
Study of human nasal epithelial stem or progenitor cell growth and differentiation in an in vitro system
... results indicated that this cell model could simulate the in vivo pathophysiological changes of NP in vitro In conclusion, we established an in vitro model for validating and comparing hNESPCs morphology ... morphology and functional activity in the cells from NP and healthy controls, and demonstrated that the intrinsic factors related to cell growth and differentiation may explain the mechanism underlying ... impairment in hyperplasia epithelium in NP, Dr Gao Tian from Haerbin medical University (Heilongjiang, China), Dr Jin Peng and Dr Duan Chen from Shandong University (Shandong, China) help me a lot in...
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Báo cáo khoa học: A comparative analysis of the transcriptome and signal pathways in hepatic differentiation of human adipose mesenchymal stem cells doc
... transcript variant Collagen, type IV, a6, transcript variant B Collagen, type XVIII, a1, transcript variant Laminin, a3, transcript variant Laminin, a5 Laminin, c2, transcript variant Vitronectin ... pivotal factor in determining stem cell transdifferentiation Discussion AT-MSCs may be good candidates as stem cells for cell transplantation and tissue engineering in regenerative medicine, as a ... plays an important role in endoderm specification and subsequent hepatocyte differentiation in vivo and in vitro [28,29] In this study, induction of HNF3b ⁄ FOXA2 expression was clearly seen in AT-MSC-Hepa...
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báo cáo hóa học:" BRCA1/2 genetic background-based therapeutic tailoring of human ovarian cancer: hope or reality?" ppt
... the non-homologous end joining (NHEJ) and single-strand non-homologous end-joining annealing (SSA), which are error-prone DNA repair pathways Deficiency in DSB repair plays a crucial role in the ... poly(ADP)-ribose and leads to a series of linear or branched polymers of PAR [21] PARP1 contains three functionally distinct domains: an amino-terminal DNAbinding domain (DBD), an auto-modification domain ... role in regulation of cell death and survival and represents an important regulatory factor in the molecular events leading to the development of cancer or inflammatory disease In vitro and in...
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Báo cáo y học: " Gene expression profiling of human prostate cancer stem cells reveals a pro-inflammatory phenotype and the importance of extracellular matrix interactions" pps
... Wnt/beta-catenin signaling in cancer stemness and malignant behavior Curr Opin Cell Biol 2007, 19:150-158 Verras M, Sun Z: Roles and regulation of Wnt signaling and beta-catenin in prostate cancer Cancer ... Functional annotation of the cancer stem cell signature by GSEA led us to four main pathways: JAK-STAT signaling; cell adhesion and extracellular matrix-interactions; focal adhesion signaling; and ... the second cycle in vitro transcription for cRNA amplification and labeling The Affymetrix eukaryotic sample and array processing standard protocol was followed at this stage and the quality of...
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Tài liệu Báo cáo khoa học: Two novel variants of human medium chain acyl-CoA dehydrogenase (MCAD) K364R, a folding mutation, and R256T, a catalytic-site mutation resulting in a well-folded but totally inactive protein pptx
... ferriceniumÆmg)1Æmin)1) (as determined by Direct Linear and Wilkinson methods), and kcat (s)1) for octanoyl-CoA oxidation Km Vmax kcat Wild-type MCAD Direct linear Confidence limits (68%) Wilkinson Standard error ... added during the purification, and certainly does not indicate the level of contamination by other protein, to judge from SDS ⁄ PAGE The ratios for R256T (8.8) and K364R (9.6), both handled in exactly ... by removing two reducing equivalents from the fatty acyl substrate, and donating them to electron-transferring flavoprotein (ETF), which ultimately feeds them to the electron transport chain to...
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Báo cáo y học: " Inhibition of constitutively active Jak-Stat pathway suppresses cell growth of human T-cell leukemia virus type 1-infected T-cell lines and primary adult T-cell leukemia cells" potx
... activation of Jak and Stat in AG490 inhibits constitutive activation of Jak and Stat in HTLV-1-infected T-cell lines and primary ATL cells (A and B) HTLV-1-infected T-cell lines were treated with increasing ... Japan) The anti-Cdk4, antiCdk6, anti-p53, anti-p21, anti-c-Myc, anti-Bcl-2 and antiactin antibodies were from NeoMarkers (Fremont, CA) The anti-Stat5 and anti-Bcl-xL antibodies were from BD transduction ... http://www.retrovirology.com/content/3/1/22 involved in the induction of Stat3 and Stat5 phosphorylation in T-cells AG490 reduces constitutive activation of Stat3 and Stat5 through inhibition of Jak kinases in HTLV-1-infected...
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Báo cáo y học: "nalysis of the mouse embryonic stem cell regulatory networks obtained by ChIP-chip and ChIP-PET" pptx
... previous findings in both mouse and human ES cells [10,12], Gene Ontology (GO) analyses revealed that a significant number of promoters occupied by OCT4 and NANOG contained transcription factors and ... overlapping reads in OCT4, and three or more overlapping reads in the case of NANOG Gene exons and introns are represented by pink boxes and solid horizontal lines, respectively For each visualized ... and NANOG targets on chromosome 19 Additional data file is a figure depicting an example of inconsistency in combining expression-profiling data with binding information from ChIP-chip and ChIP-PET...
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The impact of human resource management practices on employee's job satisfaction in Vector Aviation Co., Ltd
... collecting and recording job information, checking the job information for accuracy, writing job description based on the information, using the information to determine what skills, abilities, and ... creating a shared vision of the purpose and aims of the organization, helping each employee understand and recognize their part in contributing to them, and in so Other definitions about performance ... ANALYSIS AND FINDINGS 26 INTERVIEW DATA ANALYSIS AND FINDINGS 26 5.1.1 Data analysis 26 5.1.2 Findings 27 vi 5.2 DATA ANALYSIS FOR THE MAIN SURVEY AND FINDINGS...
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ASSESSMENT OF THE BENEFITS OF EXTENDING THE TROPICAL RAINFALL MEASURING MISSION A PERSPECTIVE FROM THE RESEARCH AND OPERATIONS COMMUNITIES doc
... factors that decision makers should consider in making an informed decision including: possible legal considerations, policy considerations, and international considerations Barring any impediments ... brightband The rain type can be classified into convective and stratiform regions that can be described by different mean vertical motion distributions and growth mechanisms Convective and stratiform ... information on tropical rainfall; insight on the physical processes of precipitation formation; unique, fine-scale information on hurricane and typhoon structure linked to rapid intensification; calibration...
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Báo cáo lâm nghiệp: "Discrimination of coated carbide tools wear by the features extracted from parallel force and noise level" potx
... with an increase in the clearance wear Regression equation and its correlation coefficient for the linears in Figures and are summarized in Table IV The regression equations and correlation coefficients ... Axelsson B.O.M., Grundberg S.A., Gronlund J.A., Tool wear when planning and milling, Measurement methodology and influencing factors, in: Proceedings of the 11th International Wood Machining Seminar, ... application to the in- process monitoring of wear, in: Proceedings of the 11th International Wood Machining Seminar, 1993, pp 147–156 [8] Lemaster R., Tee L., Monitoring tool wear during wood machining...
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Báo cáo y học: " Early detection of Varicella-Zoster Virus (VZV)-specific T-cells before seroconversion in primary varicella infection: case report" potx
... subclinical varicella or in the absence of detectable VZV DNA in plasma Consent Written informed consent was obtained from the patient for publication of this case report and any accompanying ... interpretation of clinical data All authors read and approved the final manuscript Submit your next manuscript to BioMed Central and take full advantage of: • Convenient online submission • Thorough ... radioimmunoassay: antibody responses to varicella and herpes zoster infections J Clin Microbiol 1980, 12:367-374 Cohen PR: Tests for detecting herpes simplex virus and varicella-zoster virus infections Dermatol...
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Báo cáo y học: " Identification of super-infected Aedes triseriatus mosquitoes collected as eggs from the field and partial characterization of the infecting La Crosse viruses" pot
... SI+ and I+ mosquitoes and analyzed NT and AA variability in the respective phenotypes MKB participated in mosquito rearing and IFA phenotyping of mosquitoes and in manuscript preparation and ... random in particular populations, and cause SI+ infections in mosquitoes in geographic islands There is significant gene flow in Ae triseriatus populations [41] including in our study area in ... supervised laboratory and virologic investigations, analyzed data, and prepared the manuscript WCB over saw and conducted molecular evolution and phylogenetic analyses and prepared the manuscript All...
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Báo cáo y học: " Peripheral infusion of rat bone marrow derived endothelial progenitor cells leads to homing in acute lung injury" pps
... limitations of such studies Conclusion Figure Localisation of EC and EPC in subcutanous matrigel Localisation of EC and EPC in subcutanous matrigel Confocal fluorescence microscopic examination of ... group in forward and side scatter is shown in Panel A as the green cell group Panel A and B contain furthermore as a red group a standard dose of control beads in order to quantify cells Panel ... (Griffonia simplicifolia) BS-I (green) – Orange reveals staining for red and green) F(a): Staining of EPC in culture with DAPI (blue) and FITC-labeled lectin from Bandeiraea simplicifolia (Griffonia...
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Báo cáo y học: "Sequestration and homing of bone marrow-derived lineage negative progenitor cells in the lung during pneumococcal pneumonia" ppsx
... circulation, their sequestration in the BM and lung, and their migration into airspaces in a mouse model of pneumonia Methods Animals Female C57BL/6J mice (10–12 weeks old) were used as donors and ... These cells may play an important role in inflammatory responses against lung infection as well as contributing in tissue repair processes following the infection Further studies will be required ... the study and made substantial contributions to conception, design and drafting of the manuscript All authors read and approved the final manuscript Acknowledgements We would like to thank Beth...
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Báo cáo y học: " A comparative study of a theoretical neural net model with MEG data from epileptic patients and normal individuals" docx
... firing threshold which, when exceeded, results in spike discharge, two anatomical situations can be contrasted: one in which only a few synaptic contacts reach the cell in question, and a second ... = understanding of learning, memory, understanding etc Widely used models include the pioneering work of McCulloch and Pitts [18], which treats assemblies of neurons as logical decision elements, ... using a reference system This system is based on the International 10–20 Elec- trode Placement System [13] and uses any one of the standard EEG recording positions as its origin; in this study we...
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Báo cáo y học: " Effect of neutrophil elastase and its inhibitor EPI-hNE4 on transepithelial sodium transport across normal and cystic fibrosis human nasal epithelial cells" pot
... hNE on transepithelial Na+ transport across control and CF HNEC treated with aprotinin HNEC from control (panel A) and CF (ΔF508) (panel B) patients grown on Snapwell filters and mounted in Ussing ... non CF and CF patients are presented in Table We first tested the effect of hNE on transepithelial Na+ transport in control and CF HNEC monolayers As shown in Figure 1, increasing concentrations ... measurements showing the effect of hNE in control and CF HNEC incubated with aprotinin HNEC from control (WT) and CF (ΔF508) patients grown on Snapwell filters were mounted in Ussing chamber and incubated...
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Báo cáo y học: "Low temperature tolerance of human embryonic stem cells"
... occupational exposure to densely ionizing radiation: An mFISH study Genes, Chromosomes and Cancer 2005; 44 (1): 1-9 15 Trounson A and Gardner DK Handbook of in vitro fertilization Boca Raton : CRC ... Aqua, and DAPI Images were captured, processed, and analyzed using ISIS mBAND/mFISH imaging software (MetaSystems GmbH, Altlussheim, Germany) In the mFISH technique, each chromosome (1–22 and X and ... durations (24h and 48h) It was assumed that hESC not proliferate upon exposure to low temperature This assumption is based on previous studies which demonstrated actin and tubulin depolymerization...
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Tài liệu Báo cáo khoa học: Reversible tetramerization of human TK1 to the high catalytic efficient form is induced by pyrophosphate, in addition to tripolyphosphates, or high enzyme concentration ppt
... concentration of recombinant human TK1 on oligomerization and kinetics The column and dilution buffer used and the assay performed are described in Fig (A) 40 lg was applied at a concentration ... Berenstein D & Munch-Petersen B (2004) Effect of valine 106 on structure–function relation of cytosolic human thymidine kinase Kinetic properties and oligomerization pattern of nine substitution mutants ... Dextran used as an internal standard for determination of the void volume, V0, in the individual experiments Prior to injection, hTK1 was diluted to lgÆmL)1 and incubated with mM of the indicated...
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Tài liệu Báo cáo khoa học: Transcriptional upregulation of inflammatory cytokines in human intestinal epithelial cells following Vibrio cholerae infection pptx
... isolates belonging to different serovars and biovars, including CT-producing and nontoxinogenic (CT–) strains of both environmental and clinical origin (Table 2) Cytokine mRNA expression was examined ... Cytokine response in infected epithelial cells A Bandyopadhaya et al Fig Induction of cytokine expression in intestinal epithelial cell lines by V cholerae (A) Int407 and T84 cells were infected ... another major anti -in ammatory cytokine, IL-10, following V cholerae infection in Int407 cells, account for a predominant proinflammatory cytokine response in this system The findings of this investigation...
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