biology of dendritic cells
báo cáo hóa học:" Protective CD8+ T-cell responses to cytomegalovirus driven by rAAV/GFP/IE1 loading of dendritic cells" pdf
... HLA-class I (W6/32) of antibodies, at a concentration of 25 μg/mL, were pre-incubated with the target cells for 30 minutes before addition of the stimulated T -cells K562 cells were used as targets ... use of GM-CSF and IL-4 during adher- Figure Immunofluorescence on HEK293 cells Immunofluorescence on HEK293 cells Microphotographs show fluorescent labeling for AAV/IE1 (A, B) in HEK293 cells ... or pSH3, using HEK293 cells as described previously [28,30,32] Lysates of HEK293 cells were used as virus-negative controls for mock infections Immunofluorescence HEK293 cells were spun in a cytospin...
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báo cáo hóa học: " Nogo receptor is involved in the adhesion of dendritic cells to myelin" ppt
... implications for our understanding of how immune cells act within CNS inflammatory lesions Page of 12 Methods Generation of human monocyte-derived dendritic cells Whole human blood was obtained ... of × 106 cells/ ml/well in a 24-well plate, with 20 ng/ml GM-CSF On day 6, cells were fed by removal of 75% of the medium and adding back the same volume containing GM-CSF For the generation of ... differentiation of B cells It is expressed in many cell types, including monocytes, DCs, neutrophils, stromal cells, activated T cells, B cells, B cell tumours and epithelial cells [49] Thus,...
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Báo cáo khoa học: "Altered maturation of dendritic cells by taxol, an anticancer drug" ppt
... direct effect of taxol on the interaction of DC/T cell, taxol was added into the culture of allogeneic T cells with non-pretreated DCs (B) After day culture, the number and viability of T cells were ... number and viability of T cells were measured by trypan blue exclusion test Assessment of cytotoxicity by MTT Assay The viability of DCs was measured by using MTT assay Briefly, cells were seeded ... receptors, cells were incubated with purified anti-mouse CD16/CD32 mAb (BD PharMingen) at a concentration of µg/100 µl/106 cells for 15 at 4oC Cells were incubated with each mAb at a concentration of...
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Báo cáo khoa học: "The role of Bcl-xL and nuclear factor-kB in the effect of taxol on the viability of dendritic cells" ppsx
... molecular timer regulates the lifespan and immunogenicity of dendritic cells Nat Immunol 2004, 5, 583-589 Joo HG Altered maturation of dendritic cells by taxol, an anticancer drug J Vet Sci 2003, 4, ... density of each band was divided by that of the β-actin band, and the ratio at h was set at 100% (B) Fig NF-κB involvement in the taxol-induced effects on dendritic cells (DCs) The mobilization of ... study of Bcl-xL using siRNA may further confirm their role of taxol-treated DCs Although the expression of Bax was increased in TaxolDCs, the expression of Bax occurred later than that of Bcl-xL,...
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Báo cáo y học: "Abnormal costimulatory phenotype and function of dendritic cells before and after the onset of severe murine lupus" pot
... Shortman K: The surface phenotype of dendritic cells purified from mouse thymus and spleen: investigation of the CD8 expression by a subpopulation of dendritic cells J Exp Med 1992, 176:47-58 17 ... levels of CD40 Percentages of B cells positive for CD40 from mice to months old (a) and to CD40 weeks old (b) are shown B cells were gated as B220/CD19-positive and CD11c-negative cells Results of ... 0.006 We calculated CD80/CD86 ratios, dividing the percentage of dendritic cells positive for CD80 by the percentage of dendritic cells positive for CD86 in each mouse (ex vivo) analyzed Ratios...
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Báo cáo y học: "Recruitment of dendritic cells and macrophages during T cell-mediated synovial inflammation" doc
... by exclusion of Trypan blue Immunofluorescence staining of cells Staining for dual-color immunofluorescence was performed on aliquots of to × 105 cells by means of a combination of indirect and ... adoptive transfer of AA to investigate the effects of a pulse of pathogenic T cells on recruitment of mDC-like cells to inflamed synovial tissues Data are presented also on recruitment of other blood-derived ... transfer of arthritis, expressed as proportion of cells positive for each marker (c) and numbers of cells expressing the markers (d) MHC, major histocompatibility complex ment of monocytes and PMN cells...
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The Biology of NKT Cells Albert Bendelac - part 1 ppsx
... nature of this compound, its strong effect on liver metastasis, and its activation of dendritic cells (DCs) independent of MHC class I or class II (49) led to the identification of Vα14 NKT cells ... past 10 years, more than 300 of which were published in the past year alone We attempt to organize a critical understanding of the general biology of NKT cells, mainly of the predominant mVα14 and ... Sphingomonas 298 Several lines of research led to the identification of NKT cells as a separate lineage of T lymphocytes The first sightings included (a) the identification of a canonical Vα14Jα18 (...
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The Biology of NKT Cells Albert Bendelac - part 2 pdf
... between the development of NKT cells and that of conventional T cells Furthermore, during these stages a DN population arises by downregulation of CD4 in ∼30%–50% of the cells, as shown in cell ... expression of high levels of GFP (green fluorescent protein) by the CD44low NK1.1neg and CD44high NK1.1neg cells of IL-4-GFP “4get” knockin mice, and in the expression of high levels of YFP (yellow ... membrane of the cell walls of Sphingomonas and Escherichia coli The inner leaflet of the outer membrane is composed of phospholipids, whereas the outer leaflet is made of LPS for E coli In the case of...
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The Biology of NKT Cells Albert Bendelac - part 3 pptx
... suggested a profibrotic role of Vα24 NKT cells in hepatitis C (85) and the accumulation of non-Vα24 CD1d-restricted T cells (210) Although a specific role of Vα14 NKT cells in HSV infection remains ... reinterpreted, complicating the task of drawing a clear picture of the involvement of NKT cells in noninfectious diseases Type I diabetes The relative deficiency of NKT cells in NOD mice (36, 37), combined ... However, studies of CD1d-deficient lupus-prone mice have not yielded concordant results (226–228), and injections of αGalCer ameliorated or www.annualreviews.org • Biology of NKT Cells 319 ANRV306-IY25-12...
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The Biology of NKT Cells Albert Bendelac - part 4 ppsx
... Sustained expansion of NKT cells and antigen-specific T cells after injection of α-galactosyl-ceramide loaded mature dendritic cells in cancer patients J Exp Med 201:1503–17 192 Goff RD, Gao Y, Mattner ... αβ-dependent pathway regulates thymic emigration of Vα14 invariant natural killer T cells Proc Natl Acad Sci USA 103:9160–65 www.annualreviews.org • Biology of NKT Cells 331 ARI 11 February 2007 12:20 174 ... production by dendritic cells and IL-12 receptor expression on NKT cells J Exp Med 189:1121–28 177 Carnaud C, Lee D, Donnars O, Park SH, Beavis A, et al 1999 Cutting edge: crosstalk between cells of the...
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Báo cáo y học: "Vpx rescues HIV-1 transduction of dendritic cells from the antiviral state established by type 1 interferon" pps
... effect of Vpx on HIV-1 transduction of myeloid cells after an antiviral state had been established by administration of exogenous type IFN Page of 16 Human monocyte-derived dendritic cells (MDDC) ... Transfusion Center of the Hematology Service of the University Hospital of Geneva by agreement with the Service, after approval of our project by Ethics Committee of the University Hospital of Geneva ... intravaginal inoculation and infects intraepithelial dendritic cells Journal of virology 2000, 74:6087-6095 Sewell AK, Price DA: Dendritic cells and transmission of HIV-1 Trends in immunology 2001, 22:173-175...
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Photoimmunotherapy of melanoma via combination of hypericin photodynamic therapy and in vivo stimulation of dendritic cells by PNGVL3 HFLEX plasmid DNA
... findings of Korbelik and Dougherty, 1999 whereby depletion of CD8+ T cells substantially impaired the ability of PDT to suppress the long-term growth of EMT6 as opposed to the depletion of CD4+ T cells ... Photoimmunotherapy of murine melanoma via combination of hypericinphotodynamic therapy and in vivo stimulation of dendritic cells by pNGVL3hFlex plasmid DNA Oral presentation at the 10th World Congress of the ... modality of treatment is the main focus of the present study and will be discussed in detail later Intra-tumoral injection of DCs offers the theoretical advantage of in vivo loading and activation of...
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Tài liệu Báo cáo khoa học: A peptide containing a novel FPGN CD40-binding sequence enhances adenoviral infection of murine and human dendritic cells doc
... experiments, SKBR3 cells at 90–95% confluence in a 6-well plate were transfected with lg of pRSV-mCD40 (gift of G Bishop, University of Iowa) using Lipofectamine 2000 (Invitrogen) in the presence of FBS Media ... Propidium iodide (PI) negative (viable) cells were gated as GFP-positive based on fluorescence of uninfected cells The percentage of GFP positive cells is indicated for cells infected with AdV-GFP/ FPGN2-KBP, ... as determined by fluorescence of uninfected cells Some 43.7% of CD40 transfected cells were determined to be GFP positive vs 12.6% of mock-transfected cells DC (Fig 3B) In contrast, the scrambled...
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Tài liệu Báo cáo Y học: Presence and regulation of the endocannabinoid system in human dendritic cells potx
... maturation of dendritic cells induce a series of events that lead to changes in dendritic cell phenotype and function, we have compared the amount of these compounds in immature dendritic cells that ... modulation of CB1, CB2 and FAAH mRNA expression in immature vs mature dendritic cells, we compared the expression of these genes by RT-PCR in immature dendritic cells, used as controls, with that of dendritic ... also detected in human dendritic cells RT-PCR amplification of cDNA of these cells shows a single band of the expected molecular size (Fig 2A) We also determined the presence of the FAAH protein...
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Báo cáo khoa học: Differential post-translational modification of CD63 molecules during maturation of human dendritic cells potx
... Fig CD63 isoforms in immature and mature dendritic cells (A) Immature dendritic cells, mature dendritic cells (DC) and Mel JuSo cells incubated for 48 h in the absence or presence of IFN-c were ... immature and mature dendritic cells, the amount of the 34 kDa form of CD63 disappeared gradually during the chase period, concomitantly with the appearance of isoforms of CD63 of 50 and 70 kDa, ... indicate that the 70 kDa isoform of CD63 is a result of post-translational modifications of the 34 kDa isoform that occurred exclusively in mature dendritic cells Characterization of post-translational...
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báo cáo hóa học:" In vitro generation of cytotoxic and regulatory T cells by fusions of human dendritic cells and hepatocellular carcinoma cells" docx
... of dendritic cells J Immunol 2005, 174:7764-7772 Li L, Li SP, Min J: Hepatoma cells inhibit the differentiation and maturation of dendritic cells and increase the production of regulatory T cells ... of fusion cells and have a negative impact in the stimulation of T cells To determine the induction of WT1-specitic CD8+ T cells, a pentameric assay of soluble class I MHC-peptide com- Page of ... Induction of HCC cells- specific CTL by DCs/allo-HCC To determine whether HCC cells- reactive T cells are induced by fusion cells, T cells from healthy donors were stimulated by fusions of DCs from...
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báo cáo hóa học:" Highly efficient transduction of human plasmacytoid dendritic cells without phenotypic and functional maturation" docx
... concentration of 2–5 × 106 of cells per 200–500 μl of medium After hours at 37°C, cells were placed in complete medium and analysed by flow cytometry between day and day 60 Transduction of CD34-pDC ... cells and AAV vectors are mainly episomal A single exposure of pDC to rAAV2/1, rAAV2/2 or rAAV2/5 led to very low levels of transduced cells ranging from around 3% to less than 1% of E-GFP+ cells ... Liu YJ, Pulendran B, Palucka K: Immunobiology of dendritic cells Annu Rev Immunol 2000, 18:767-811 Pulendran B: Modulating vaccine responses with dendritic cells and Toll-like receptors Immunol...
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Báo cáo hóa học: " Molecular signatures of maturing dendritic cells: implications for testing the quality of dendritic cell therapies" pptx
... results of result of gene expression profiling Jin et al Journal of Translational Medicine 2010, 8:4 http://www.translational-medicine.com/content/8/1/4 Page of 15 Figure Change in the expression of ... These Treg cells can counter the effects of Th1 responses by cytotoxic T cells, Th1 cells, and NK cells In contrast, we found that LPS and IFN-g maturated DCs did not increase the levels of CCL22 ... analysis of maturing DCs revealed two distinct patterns: down-regulation of groups of miR at hours of maturation with sustained low expression throughout the rest of maturation and up-regulation of...
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Báo cáo hóa học: "Multicenter phase II study of matured dendritic cells pulsed with melanoma cell line lysates in patients with advanced melanoma" potx
... results of immune monitoring and clinical benefit in terms of tumor responses This lack of correlation of immune parameters and response may relate to the likely low sensitivity of the detection of ... Detection of T -cells specific for tumor antigen epitopes was performed after IVS or directly ex-vivo after thawing PBMC samples Activation of specific T -cells was monitored by production of IFN-g ... beyond weeks) rate of 20%, a null response rate of 5%, a type one error of 0.10, and a type two error of 0.10 As such, continuation to the second stage required one patient out of the first 12 recruited...
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báo cáo hóa học:" Comparative study of clinical grade human tolerogenic dendritic cells" potx
... DC-tols not promote a Th1 profile To analyze the effect of the different tol-DCs, allostimulated T cells were further studied An example of the Page of 14 proliferation of T cells allostimulated by ... centre stage of active immunotherapy [16-23] Dendritic cells maintain immune tolerance to self-antigens by deleting or controlling the pathogenicity of autoreactive T -cells Modifications of DCs in ... these T cells in co-cultures of MDDCs with allogenic T cells for one round of stimulation However, it has been demonstrated that VitD3-DCs convert naive T cells into Tregs after several rounds of...
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