biochemical characterization and genetic basis
Tài liệu Báo cáo khoa học: Pyrimidine-specific ribonucleoside hydrolase from the archaeon Sulfolobus solfataricus – biochemical characterization and homology modeling doc
... hypoxantine, guanosine and guanine, uridine and uracil, cytidine and cytosine were 12.2 and 6.2 min, 10.5 and 4.7 min, 15.2 and 6.1 min, 6.8 and 4.2 and 6.6 and 3.9 respectively The amount of purine ... employed and the elution was carried out with : 94 (v v) mixture of 95% methanol and 0.1% triuoroacetic acid in H2O The retention times of adenosine and adenine, inosine and hypoxantine, guanosine and ... A, we were able to calculate the volume of buried and surface cavities for SsCUNH and the template, both in the monomer and in the tetramer, and we found that the volume of buried cavities found...
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Tài liệu Báo cáo khoa học: Biochemical characterization and inhibitor discovery of shikimate dehydrogenase from Helicobacter pylori docx
... H pylori shikimate dehydrogenase (HpSDH) was cloned, expressed, and purified in E coli system, and its biochemical and enzymatic characterizations were also carried out Furthermore, by using the ... (Eqn 2) On the other hand, compounds 1, 4, and are noncompetitive inhibitors, and compounds and are competitive inhibitors with respect to NADP Table summarizes the IC50 values and kinetic inhibition ... 15.4 In conclusion, we have firstly cloned and expressed HpSDH enzyme, and the biochemical characterization of HpSDH is expected to favor better understanding the SDH features Moreover, by high...
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Báo cáo Y học: The cold-active lipase of Pseudomonas fragi Heterologous expression, biochemical characterization and molecular modeling pdf
... peptide-independent pathway and calls for further investigation on this subject Enzyme activity and specificity In the standard tricaprylin hydrolysis assay, rPFL displayed highest activity at 29 °C and pH 8.0, ... substrate binding funnel (V123 with L119 and F249 with R224) and within the binding pocket where T18, F119, A247 and T251 in BCL are substituted in PFL by F, L, N and V residues, respectively Site-directed ... strain B11-1: gene cloning and enzyme purification and characterization Appl Environ Microbiol 64, 486–491 Feller, G., Thiry, M., Arpigny, J.L & Gerday, C (1991) Cloning and expression in Escherichia...
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Bài thuyết trình về: "Alkali and Halotolerant Catalase from Halomonas sp. SK1: Overexpression in Escherichia coli, Purification, Characterization, and Genetic Modification"
... KHẢO Phucharoen, K., Hoshino, K., Takenaka, Y., and Shinozawa, T., Purification, characterization, and gene sequencing of a catalase from an alkali- and halo-tolerant bacterium, Halomonas sp SK1 ... gấp cuộn,… (Yujin E Kim,∗ Mark S Hipp,∗ Andreas Bracher, Manajit Hayer-Hartl, and F Ulrich Hartl 2013 Molecular Chaperone Functions in Protein Folding and Proteostasis Annual Review of Biochemistry ... (2002) Molecular Chaperone Functions in Protein Folding and Proteostasis.Yujin E Kim,∗ Mark S Hipp,∗ Andreas Bracher, Manajit Hayer-Hartl, and F Ulrich Hartl Annu Rev Biochem 2013 82:323–55 This...
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Biochemical characterization of thermophilic lignocellulose degrading enzymes and their potential for biomass bioprocessing
... (filer paper), BWX and PWSD The enzyme kinetic studies for cellulase and xylanase (Km and Vmax) were performed with 1-10 mg/ml of CMC and BWX, respectively [35] The crude cellulase and xylanase were ... albumin as standard [38] All experiments were run in duplicate and standard deviations (SD) were calculated using Microsoft Excel and results were presented as average ± SD Results and discussion ... degradation and conversion for sustainable production of bioenergy and highvalue products; enzyme production and catalysis, bioremediation and biorefineries Lew is the author of patents and over...
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Tài liệu Báo cáo khoa học: Tissue expression and biochemical characterization of human 2-amino 3-carboxymuconate 6-semialdehyde decarboxylase, a key enzyme in tryptophan catabolism pptx
... curves and data were normalized to the copy number of 18S RNA Expression of ACMSD isoforms in E coli pGEM-ACMSD I and pGEM-ACMSD II were digested with XhoI and Bpu1102I and cloned into pET-15b and ... swiss-pdbviewer and visual molecular dynamics [49] Acknowledgements We thank G Magni and S Ruggieri for helpful comments and suggestions and T Begley (Department of Chemistry and Chemical Biology, ... Escherichia coli and Pichia pastoris ACMSD I and II cDNAs were subcloned into pET15b and pET32b E coli expression vectors, providing the recombinant proteins with a N-terminal His6-tag, and a thioredoxin-tag,...
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Tài liệu Báo cáo khoa học: Structural and biochemical characterization of a human adenovirus 2/12 penton base chimera pptx
... insertion of $ 230 residues (residues 133–367) between strands D and E and a smaller insertion of $ 50 residues (residues 404–458) between strands F and G The hypervariable loop, FEBS Journal 273 (2006) ... expression, the SF21 and Hi5 cell lines and the Bac-to-Bac expression system and vectors were from Invitrogen (Carlsbad, CA, USA) Protease inhibitors were from Roche (Basel, Switzerland) and Ni-NTA resin ... penton base (yellow), hAd2 ⁄ 12 (blue), and hAd2 fiber peptide complex (pink) The fiber peptide is drawn as ball -and- stick and colored by atom fiber peptide bound and unbound structures reveal a high...
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Tài liệu Báo cáo khoa học: Biochemical characterization of human 3-methylglutaconyl-CoA hydratase and its role in leucine metabolism docx
... seems, that the fronting, the shoulder and the tailing is due to acetyl-CoA and not due to any other compound (B) The peaks 3, 4, and were isolated by HPLC and used as substrates for AUH The AUH ... protein and it was thus assumed that the mature form of human AUH in brain has a molecular weight of 32 kDa (AUHp32) [15] For the kinetic characterization of AUH described in the work at hand, AUH ... proteolysis and the resulting proteins AUHp40 and AUHp30 were again purified by chromatography Thus, four different pure fractions of AUH could be generated: MBP-AUHp40, AUHp40, MBP-AUHp32 and AUHp32...
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Tài liệu Báo cáo khoa học: Biochemical characterization of Bacillus subtilis type II isopentenyl diphosphate isomerase, and phylogenetic distribution of isoprenoid biosynthesis pathways doc
... the c-proteobacteria A vinelandii and P luminescens and to Idi-1 protein of the Bacteroid C hutchinsonii (expect values 3e-22, 5e-19 and 8e-20, respectively) Phylogenetic pattern of Idi proteins ... the GenBank database, and open reading frames were identified and translated into amino acid sequences with the program PCGENE (IntelliGenetics, University of Geneva, Switzerland) Alignments were ... conveniently by NMR spectroscopy (Table 1) The 1H NMR and 13C NMR signals of IPP and DMAPP have been assigned previously on the basis of 1H13C and 13 13 C C correlation spectroscopy with 13C-labelled...
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Tài liệu Báo cáo khoa học: Molecular and biochemical characterization ofD-phosphoglycerate dehydrogenase fromEntamoeba histolytica A unique enteric protozoan parasite that possesses both phosphorylated and nonphosphorylated serine metabolic pathways docx
... Leishmania and E caudatum among protozoa cannot be ensured Phylogenetic analysis The phylogenetic inference was performed by ML, NJ and MP methods using protein sequences from 35 PGDH and eight ... set of enzymes involved in amino acid synthesis of Ser from Gly and Ala from Cys and conversions between Asp and Asn and between Glu and Gln [49] Serine metabolic pathways are often absent in parasitic ... residue between 58 and 59 of EhPGDH (also missing in other Type III organisms and Type IIB B anthracis) in the substrate binding domain and five–ten residues between amino acid 172 and 173 (Fig 2)...
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Tài liệu Báo cáo Y học: Structural and biochemical characterization of neuronal calretinin domain I– II (residues 1– 100) Comparison to homologous calbindin D28k domain I–II (residues 1 –93) pdf
... for both CR and Calb [22,24,30] The biochemical properties of CR and Calb are similar to those of calmodulin, the best-known EF-hand calcium sensor It is difficult to localize the biochemical ... us to analyze the biochemical and structural properties of CR I– II and to compare them with Calb I–II Our data show that CR I –II and Calb I –II share virtually the same EF-hand structures However, ... underlined An EF-hand consensus sequence is placed between the aligned EF-hand I and EF-hand II sequences [3] The helices are shown as boxes with conserved hydrophobic (h) and other amino acids...
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Báo cáo khoa học: Biochemical characterization of USP7 reveals post-translational modification sites and structural requirements for substrate processing and subcellular localization pptx
... Coomassie stained and major bands were excised and eluted in order to perform N-terminal sequencing Tandem affinity purification and mass spectrometric analysis of USP7 N-terminal and C-terminal TAP-tagged ... Fernandez-Montalvan et al Biochemical characterization of USP7 Fig Enzymatic characterization of USP7 (A) Progress curves for the USP7-catalyzed hydrolysis of Ub-AMC (j), SUMO-1-AMC (d) and Nedd8-AMC ... expression vectors and USP7 cDNA as well as Shirley Gil-Parrado, Bruno Martoglio, Martin Renatus and Jorg Eder (Novartis, Basel, Switzerland) ¨ ´ for support and helpful discussions A Fernandez´ Montalvan...
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Báo cáo khoa học: Glycolysis in Entamoeba histolytica Biochemical characterization of recombinant glycolytic enzymes and flux control analysis ppt
... glycolysis E Saavedra et al in which Vf and Vr represent the maximal forward and reverse rates, Ks and Kp are the Michaelis constants for substrate s and product p, and kz is the rst-order rate constant ... analysis showed CJ Ei values of 0.08 and 0.2 for PGAM and 0.85 and 0.57 for PPDK at 10 and 39 mU of added PPDK, respectively PGAM was 91 mU, ENO was 309 mU and LDH was 11 U; ENO exerted no ux ... glucose, 0.3 mm ATP and the reaction was started with 0.290.38 U EhHK The Km values for glucose and ATP were determined by varying the concentrations between 0.01 and mm, and 0.01 and mm, respectively...
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Báo cáo Y học: Purification and biochemical characterization of some of the properties of recombinant human kynureninase pptx
... plotted using the CRICKETGRAPH and GraphPad PRISM3 software packages, and the kinetic parameters Km and Vmax were obtained using non-linear regression Lineweaver–Burk and Dixon [14] plots were used ... reducing agents SDS and mercaptoethanol to determine the native dimeric molecular mass resulted in the appearance of two bands at 52.5 and 95 kDa (gel image not shown), and this shows that ... spectrofluorimetrically at 37 °C, with excitation of the product 3-hydroxyanthranilate at 330 nm and emission at 410 nm and 310 nm and 417 nm respectively for anthranilate, by the method of Shetty & Gaertner [7]...
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Báo cáo Y học: Structural and biochemical characterization of calhepatin, an S100-like calcium-binding protein from the liver of lungfish (Lepidosiren paradoxa) docx
... crucial for dimerization and monomer stability, in Sl00 A4 and other S100 proteins [23] (Fig 3), are clustered between helix I and IV (Fig 5B), in the same way as in Sl00 A4 and other S100 proteins ... the crystal and/ or NMR structures of other members of the family The molecule is depicted in strand representation, and a helices are numbered from I to IV (B) Residues Leu7, Arg8 and Phe11 from ... a-helix I, and Trp60, Phe63, Ala66 and Phe67 from a helix IV (located at equivalent positions to those crucial for S100 A4 dimerization and monomer stability [23]) are shown in dark and light...
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Báo cáo khoa học: Functional and genetic characterization of the promoter region of apolipoprotein H (b2-glycoprotein I) ppt
... population and 15 mgÆdL)1 in African Americans [12], which may have clinical relevance in b2GPIrelated pathways Family and heritability data have provided strong support for the genetic basis of ... restriction enzymes KpnI and BamHI at the 5¢ and 3¢ ends of each deleted fragment, respectively The PCR products were gel purified (Qiagen, Valencia, CA, USA) and then digested with KpnI and BamHI restriction ... in relation to lupus and lupus-related phenotypes J Rheumatol 36, 315– 322 18 Quandt K, Frech K, Karas H, Wingender E & Werner T (1995) MatInd and MatInspector: new fast and versatile tools for...
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Báo cáo khóa học: Identification of a gene encoding Lon protease from Brevibacillus thermoruber WR-249 and biochemical characterization of its thermostable recombinant enzyme pptx
... used as the standards and their elution volumes, Ve, were determined The standard curve was plotted with the logarithm of molecular mass against Ve/V0 of the standard protein Peptidase and ATPase ... phosphatase Materials and methods Bacterial identification and culture conditions DNA manipulation and sequence analysis Plasmid DNA preparation, purification of DNA from agarose gel, and restriction ... was extracted from Br thermoruber by standard methods [23] and digested with HindIII and SalI The digested DNA fragments were ligated with cassette adaptors and then used as a template for the following...
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Báo cáo khoa học: An a-proteobacterial type malate dehydrogenase may complement LDH function in Plasmodium falciparum Cloning and biochemical characterization of the enzyme potx
... manufacturer’s protocol Cloning of Pf MDH, Pf LDH and Pf MQO Pf MDH was cloned for functional and biochemical characterization The other two genes Pf LDH and Pf MQO were also cloned to obtain the DNA ... evaluation of Vmax and kcat values for malate/NAD and OAA/NADH (Table 1) The saturating concentrations of OAA and NADH were found to be 250 lM and 150 lM, respectively, while for malate and NAD these ... blotting and also by quantification of the enzyme protein by Western blotting Expression of Pf MDH, Pf LDH and Pf MQO in control and drug treated P falciparum cultures was evaluated by Northern and...
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Báo cáo khoa học: Identification and biochemical characterization of the Anopheles gambiae 3-hydroxykynurenine transaminase pot
... identification, the bacterial heterologous expression and the biochemical characterization of 3-HKT from A gambiae These results represent the first biochemical report concerning an enzyme 5654 F Rossi ... a better understanding of the XA metabolism in the mosquito and offers a new possible target for the development of insecticides and ⁄ or transmission blocking agents Results and Discussion Isolation ... Within such a scenario, the isolation and biochemical characterization of recombinant A gambiae 3-HKT that we report here, represents a step forward into the understanding of XA metabolism in FEBS...
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Báo cáo Y học: Characterization of the 105-kDa molecular chaperone Identification, biochemical properties, and localization pptx
... residues, and those aminoacid sequences were the same To determine the interaction between the 105-kDa protein and APG-1 or OSP94, we investigated the amino-acid sequence and some biochemical ... (v/v) with 25% isopropyl alcohol (v/v) and 10% acetic acid (v/v) and destained with 10% isopropyl alcohol (v/v) and 10% acetic acid (v/v) Ó FEBS 2002 Biochemical properties of the 105-kDa protein ... libitum access to water, and dehydrated rats were restricted from drinking water for and days All rats were sacrificed, the kidneys were removed, and the renal cortex, medulla, and papilla were dissected...
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