binding properties in the bean builder
Báo cáo khoa học: Starch-binding domains in the CBM45 family – low-affinity domains from glucan, water dikinase and a-amylase involved in plastidial starch metabolism pptx
... b-cyclodextrin injection, and the bottom panel depicts the binding isotherm; open circles represent the integrated binding heat of the data in the top panel, and the full line is the fit of a one-site binding ... the starch -binding ability of AtAMY3 in vitro It cannot be precluded that some binding may involve secondary binding sites in the catalytic domain, but the demonstrated starchbinding ability ... showed no binding to maltoheptaose Taken together, the data presented here show that the binding affinity of the 1180 The full-length AtAMY3 offered an advantage when examining the binding affinity...
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Báo cáo khoa học: Nuclear actin and actin-binding proteins in the regulation of transcription and gene expression docx
... cell death [65,66] The AR contains an N-terminal domain harboring activation function 1, a central DNA -binding domain (DBD) and a C-terminal ligandbinding domain (LBD) containing activation function ... ligand -binding domain Actin -binding protein Targeting sequence Gelsolin Direct or indirect association with the AR Region LBD Classes Role in the cytoplasm AR effect Mechanism ()) Actin filament ... Direct a-actinin-2 ()) Bundling proteins Coactivator Supervillin NLS F-actin- and membraneassociated scaffolding protein Filamin NLS? Cross-linking proteins Filamin A NLS? Cross-linking proteins Transgelin...
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Báo cáo khoa học: A novel retinol-binding protein in the retina of the swallowtail butterfly, Papilio xuthus docx
... species of the hydrophobicligand -binding protein and solely binds 3-hydroxyretinol as an intrinsic ligand, we termed this protein the Papilio retinol -binding protein (Papilio RBP) Further analysis ... ligand -binding protein in the fly chemosensory hair [26]) The calculated pI value of the Papilio RBP is 4.92; the protein is highly acidic This explains the high mobility of the protein in the native ... retinol -binding protein, Papilio RBP We identified a novel type of protein that binds retinol, which we termed the Papilio retinol binding protein (Papilio RBP) The native ligand of this protein...
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Báo cáo khoa học: Autoregulatory binding sites in the zebrafish six3a promoter region define a new recognition sequence for Six3 proteins potx
... topological binding mode of the HD and in uence the nucleotide contacts made by the recognition helix [6] Notably, these different binding modes seemed to depend on the binding site sequence, and the binding ... that intramolecular interactions involving residues outside of the protein–DNA binding interface can also in uence DNA recognition [10,40] In particular, variations in the position of the N-terminal ... previously reported Six3 -binding sequence (TGATAC), we observed similar binding affinity, suggesting that the Six3 HD has several binding modes with distinct DNA -binding specificities Using several experimental...
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Báo cáo khoa học: Specific Ca2+-binding motif in the LH1 complex from photosynthetic bacterium Thermochromatium tepidum as revealed by optical spectroscopy and structural modeling pdf
... also known as the EF hand [16] Proteins containing the EF hand are divided into two classes according to their functions: signaling and buffering ⁄ transport proteins The former undergoing Ca2+-dependent ... However, coordination with 6, or even up to 12 atoms is also possible A helix-loop-helix structural domain constituting the Ca2+ binding motif is found in a large number of Ca2+ -binding proteins, and ... (fwhm)] on raising the temperature from 273 to 323 K The increase in spectral shift or bandwidth in response to the increase in temperature may indicate the involvement of more thermally populated...
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Báo cáo khoa học: Co-operation of domain-binding and calcium-binding sites in the activation of gelsolin pptx
... actin -binding sites, is in interaction with the C-terminal a-helix of G6 domain The sequence 159–193, including the second actin interface, appears also in interaction with G6 domain Therefore, ... 0.5–0.8 lM) in EGTA, weaker binding was evident in the presence of mM calcium (Fig 5) In addition a change in the interface occurs during calcium binding to IIG6 site (Fig 6) The interaction ... G2 domain Footprint of G2 on gelsolin G4–6 domain Two sites within the G2 domain appear involved in the G2- G4–6 interface in the presence of EGTA (Fig 3) The interaction of these two sites (residues...
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Báo cáo Y học: Barley a-amylase Met53 situated at the high-affinity subsite )2 belongs to a substrate binding motif in the bfia loop 2 of the catalytic (b/a)8-barrel and is critical for activity and substrate specificity pot
... hampered by introduction of an aromatic side chain in the middle of the AMY1 binding cleft which apparently disturbed crucial steps in the mechanism, perhaps involving contacts between domains A and ... interactions in the substrate-mutant enzyme complex between subsite )2 and other parts of the binding cleft emphasized the intimate contact in between ba loop and domain B and its importance in activity ... AMY2 the predominant isozyme in malt was solved in the native state and in complex with the inhibitory pseudotetrasaccharide acarbose [15,16] Subsite mapping showed 10 substrate binding subsites...
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phase equilibria and fluid properties in the chemical industry estimation and correlation
... between academic and industrial engineers and whether the ideas expressed influence research in the coming years xii In Phase Equilibria and Fluid Properties in the Chemical Industry; Storvick, ... as in m n discussions through the years ay Beginning in 1937, I had been very m c interested in the uh thermodynamic properties of various hydrocarbon molecules and hence of those substances in ... sessions, continuations of scheduled sessions and meetings packed into the four sunny afternoons of the meeting Many important areas of work were identified as needing further attention during the next...
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Báo cáo khoa học: "Histochemical Characterization of the Lectin-binding Sites in the Equine Vomeronasal Organ" potx
... of the Lectin -binding Sites in the Equine Vomeronasal Organ 17 Fig Histochemical staining of DBA (A and B), SBA (C and D), and Isolectin B4 (E and F) in the vomeronasal sensory epithelium of the ... Microvilli Receptor cell Supporting cell Basal cell Glands DBA ＋ ＋＋ － － － SBA ＋ － － － － －, No binding; +, infrequent (66% binding Lectin Isolectin B4 WGA ＋ ＋＋ － ＋＋ － － － － － ... consisted of the receptor, supporting cells, and basal cells The J acobson's glands were situated in the lamina propria Since the ducts of these glands penetrated the epithelium and opened into the VNO...
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Báo cáo khoa học: " Interactions between Scots pine, Ips acuminatus (Gyll.) and Ophiostoma brunneo-ciliatum (Math.): estimation of the critical thresholds of attack and inoculation densities and effects on hydraulic properties in the stem" pdf
... conditions or with increasing inoculation densities [10, 14] Induced reaction zones occur both in the phloem and the sapwood in response to bark inoculation They may therefore, together with the development ... the fungus into the bark The extent of damage in the sapwood nevertheless displayed a significant increase with increasing inoculation densities while the length of reaction zones in the bark did ... brunneo-ciliatum into bark of young Scots pine trees on: (a) the length of the induced reaction zones in the bark tissues; (b) the fraction of sapwood remaining functional after inoculation; (c) the fraction...
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Báo cáo y học: "Analysis of C4 and the C4 binding protein in the MRL/lpr mouse" pptx
... RG: Free protein S levels are elevated in familial C4b -binding protein deficiency Blood 1990, 76:2527-2529 Hessing M, van TVC, Bouma BN: The binding site of human C4b -binding protein on complement ... C-reactive protein: targeting of the inhibitory activity of C4b -binding protein J Immunol 2006, 176:7612-7620 Webb JH, Blom AM, Dahlback B: The binding of protein S and the protein S-C4BP complex ... preventing the formation of the C3 convertase by binding to C4b; accelerating the natural decay of the classical pathway C3 convertase; and as a cofactor for the serine proteinase factor I in the...
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Báo cáo sinh học: " Laboratory evolution of host plant utilization in the bean weevil" pps
... by the ’windows’ on the seed testa becoming black; otherwise the windows are grey.) At that time, beans and chickpeas were separated In replicate lines (the ’choice Phaseolus lines’), bean seeds ... to find a place to enter the seeds; usually they remain underneath the seed where the eggs were laid).) In order to determine how much selected lines diverged from each other in traits affecting ... imposed The mean percentages of eggs laid on chickpeas in the Cicer lines, no matter what the treatment or the rearing host were, were higher than those in the Phaseolus lines This finding was...
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Báo cáo khoa hoc:" Despite WT1 binding sites in the promoter region of human and mouse nucleoporin glycoprotein 210, WT1 does not influence expression of GP210" pptx
... amino acid insert and the KTS insert in the zinc finger region, did not influence GP210 expression in SAOS osterosarcoma cells, in a system using tetracycline-induced repression of expression In ... determine whether the putative WT1 binding sites in the GP210 promoter might correspond to functional reg- The present study describes the genomic structure of the human nucleoporin GP210 gene, including ... for either of these proteins The 121-kDa pore membrane protein POM121 [7,8] is located in the pore membrane domain of the NPC with a short (29 residues) N-terminal tail protruding into the lumen...
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Báo cáo khoa học: " Characterization of the RNA-binding properties of the triple-gene-block protein 2 of Bamboo mosaic virus" pdf
... TGBp2 To determine whether the unfused TGBp2 binds viral RNA in a specific or non-specific manner, two non-viral Figure The RNA -binding properties of unfused TGBp2 The RNA -binding properties of ... to the RNA, resulting in quenching of the tyrosine fluorescence We then studied the effect of changing the molar ratio of the viral RNA fragment to TGBp2 on the tyrosine fluorescence quenching ... using Northwestern blot [15] However, RNA binding of TGBp2 in aqueous solution has not been studied To confirm that TGBp2 is able to bind viral RNA and to gain insight into the RNA -binding properties...
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Báo cáo y học: "Distinctive receptor binding properties of the surface glycoprotein of a natural Feline Leukemia Virus isolate with unusual disease spectrum" pps
... implicates a VRB-containing region as the major determinant of the binding phenotype A region of SU containing the VRB domain was next examined for contribution to the FeLV-945 binding phenotype First, ... domain Taken together, the comparative binding assays implicate the VRB-containing substitution as the major determinant of binding phenotype, but also indicate a contribution of the VRA domain ... positively impact SU binding in the context of VRA from either FeLV-A/61E or FeLV-945, but the optimal binding interaction is achieved in the presence of FeLV-945 VRA Within the VRB-containing segment...
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INVESTIGATION OF MEMBRANE PROPERTIES IN THE CENTRAL NERVOUS SYSTEM OF DROSOPHILA MELANOGASTER STUDIED BY FLUORESCENCE CORRELATION SPECTROSCOPY
... constituent instead of sphingomyelin (PE-cer has phosphoethanolamine as the head group, instead of the phosphocholine head group in SM) [32] Despite having shorter fatty acyl chains (the longest being ... be studied using FCS These intensity fluctuations contain information which can be extracted by analysing the rates and the amplitudes of the intensity fluctuations and subjecting them to an autocorrelation ... tube The eppendorf tube was kept cold at °C in ice After collecting 10 larval brains, they were washed times with 200 µl of sterile-filtered HL3 containing 10 µg/ml of insulin The brains were then...
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Tài liệu Báo cáo Y học: Short peptides are not reliable models of thermodynamic and kinetic properties of the N-terminal metal binding site in serum albumin doc
... and the neighbouring His242) bind phosphate ions, thereby providing another level of competition for metal ion binding Kinetics of Ni(II) binding and Cu(II)/Ni(II) exchange The data presented in ... donors at the protein surface, limited accessibility of the binding site due to shielding from the bulk of the protein, or some conformational interactions The metal-free DAHK sequence in HSA has ... binding constant, making phosphate competition negligible The kinetics of Ni(II) binding to model peptides and albumins at pH 7.4 was also monitored by CD spectroscopy In these experiments, the...
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Báo cáo khoa học: RNA-binding properties of HCF152, an Arabidopsis PPR protein involved in the processing of chloroplast RNA pdf
... when the yeast-tRNA was omitted from the binding assay, indicating a lesser specific binding to RNA Finally, the two truncated proteins containing a single PPR domain did not show any binding to ... analysis of the BDd RNA, the high affinity binding molecule Defining the binding site of HCF152 in the psbH-petB transcript In order to further characterize the HCF152 binding site, we synthesized ... with a binding buffer containing 100 mM NaCl, and the 35 S-labeled proteins that bound the resin via the His6-fused proteins were eluted with the binding buffer containing 500 mM NaCl The bound...
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Báo cáo khoa học: Odorant binding protein has the biochemical properties of a scavenger for 4-hydroxy-2-nonenal in mammalian nasal mucosa doc
... against HNE–protein adducts Ligandbinding tests showing the functionality of the same HNE-treated porcine (B) and bovine (C) OBPs as in the immunostaining The plots show the residual AMA -binding ... example, bovine OBP is peculiar in that it is a dimer with domain swapping [14,15] There is currently a lack of information on the in vivo binding properties of OBP, the low binding specificity, ... Ligand -binding tests showing the functionality of the same HNE-treated porcine (C) and bovine (D) OBP samples from the spectrophotometric titration The plots, showing the residual AMA -binding capacity...
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Tài liệu Psychometric properties of the quality of life scale Child Health and Illness Profile-Child Edition in a combined analysis of five atomoxetine trials pdf
... data from clinical trials studying atomoxetine and using the CHIP-CE in the Lilly data base were included The total number of patients included in the combined analysis was 794 Three of these studies ... Item-domain correlations Within the various CHIP-CE domains, the highest and the lowest Spearman’s correlations between the individual items and the respective domain are reported in the following The ... from baseline The same was found to be true for the Comfort domain regarding the correlation of the domain with the total score The Achievement domain, the Satisfaction domain, and the Risk avoidance...
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