... 3.4.2 Binding of peptides with C-terminal negatively charged residue………… 90 3.4.3 Binding of peptides with C-terminal positively charged residue………… 90 3.4.4 Binding of peptides with C-terminal ... moderate influences on binding specificity at the P9 anchor, and most subtypes can bind the same peptides with similar binding affinity In summary, our study has defined some major rules governing ... extended main-chain and side-chain conformations in the binding site Figure 1.6 Interaction between HLA Class I Molecules and Peptides A longitudinal section through the peptide -binding groove...
... both finger and palm domains resulting in the closed conformation of RdRp during de novo initiation This is to restrict access to the template binding channel thereby preventing dsRNA binding to ... the inhibitor binding pocket for invitro polymerase activity and as well for replication fitness in context of the DENV TSV01 infectious virus For these studies, individual amino acids lining ... polymerase-inhibitor interactions To assess the relevance of the inhibitor binding pocket for invitro polymerase activity and To assess the relevance of the inhibitor binding pocket for replication fitness in...
... differences in ZP binding registered However, the lack of binding to FT ZP is mainly due to the ZP and not to the spermatozoa, since fresh spermatozoa also had markedly decreased binding to FT ZP In line ... obtained by mincing the ovaries under a stereo microscope with a scalpel in a Petri dish filled with 0.5% bovine serum albumin (BSA) in Tyrode's albumin lactate pyruvate (TALP) solution for washing ... the opening of the tank for minutes and then at 13 cm for minutes and finally at 20 cm below the opening for minute, before plunging them into the liquid nitrogen The straws were thawed in a water...
... synthesized in one round of synthesis, we performed RdRp assays in the presence of heparin in order to prevent reinitiation In a first step we determined the heparin concentration needed to prevent reinitiation ... fragment contains two domains: domains FEBS Journal 272 (2005) 3872–3886 ª 2005 FEBS Binding and replication of 3¢-end of HCV minus RNA I and II Structures of both domain I (A), as determined by Schuster ... unannealed as in the wild-type 3875 Binding and replication of 3¢-end of HCV minus RNA T Astier-Gin et al Table RNA synthesis obtained with mutants of (–)IRES RNA without or with heparin Determination...
... activator -binding domains is also of interest in relation to the twostep binding mechanism between Gal4 and Swi1 that has been characterized invitro [34], and our results suggest that the coupled binding ... function of the increasingly large group of proteins that have been shown to contain intrinsically unstructured regions The intrinsic conformation flexibility that is inherent in this binding mechanism ... mediate interaction with transcriptional activators invitro [33] Recombinant activator interaction domains interact with activation domains invitro using a two-step mechanism, where rapid ionic interaction...
... element binding protein: cellular nucleic acid binding protein Virus Res 58, 73–82 Flink IL & Morkin E (1995) Alternatively processed isoforms of cellular nucleic acid -binding protein interact ... during 15 with different kinase inhibitors at 30 °C and then used to analyse invitro recombinant CNBP phosphorylation in kinase buffer containing 100 lm ATP and lCi [32P]ATP[cP] at 30 °C for Kinase ... band-shift intensity increased along with the protein increasing amount Nevertheless, no significant differences inbinding affinities were observed as a consequence of phosphorylation (Fig S6) This finding...
... residues involved in DNA binding activity Filled arrowheads correspond to the seven amino acid residues involved in DNA binding activity that are changed in EhTBP sequence Arrow denotes the amino ... showed that rEhTBP has an invitrobinding capacity for distinct TATA elements Quantification of rEhTBP DNA -binding activity for different TATA oligonucleotides Binding activity of rEhTBP for ... Therefore, in addition to TATTTAAA(1) sequence, we showed here that TAT_ _AAA(4), TAT_ _AAg(5) and TATTaAAA(6) are, at least in vitro, EhTBP binding motifs In addition, rEhTBP can also bind in vitro...
... These solutions were assayed against HSV-1 and HSV-2 using a cell protection assay Immortalized African Green Monkey Kidney (Vero) cells (ATCC) were grown to a monolayer in minimal essential medimum ... cells were about 40% destroyed At this point in time viral growth had only been observed in cells treated with DMSO and infected with HSV-1 However, in other solvents with 1mg/ml compound, the ... mixture Against HSV-1, it provided about 70% protection, though it was fairly ( 40%) cytotoxic Finally, 0.25 mg/ml in 2.5% ethanol provided about 70% protection against HSV-2 with minor cytotoxic...
... Effect of4 Figure inhibitors on invitro processing Effect of inhibitors on invitro processing Various concentrations of protease inhibitors were added to the invitro processing assay for hr at ... of inhibitors on invitro processing Inhibitor Name Concentration Inhibit Cleavage Metalloproteinase Aspartic acid proteinase Serine proteinase Cysteine proteinase EDTA Pepstatin PMSF E-64 E-64 ... profile Processing Kinetics of Core Protein Precursors To determine the optimal temperature and kinetics of processing of the core protein precursors in the invitro cleavage assay, a time course...
... Effect of4 Figure inhibitors on invitro processing Effect of inhibitors on invitro processing Various concentrations of protease inhibitors were added to the invitro processing assay for hr at ... of inhibitors on invitro processing Inhibitor Name Concentration Inhibit Cleavage Metalloproteinase Aspartic acid proteinase Serine proteinase Cysteine proteinase EDTA Pepstatin PMSF E-64 E-64 ... profile Processing Kinetics of Core Protein Precursors To determine the optimal temperature and kinetics of processing of the core protein precursors in the invitro cleavage assay, a time course...
... prior to RNA binding [18] Furthermore, we show that NusA-HuR binds a synthetic RNA template that contains AREs (Fig 2C), indicating that the fusion does not interfere with the RNA binding activity ... association in a yeast two-hybrid screen and confirmed the interaction by a homogenous time-resolved fluorescence bindingassay The authors mapped the HIV-1 RT-HuR binding sites to the RNase H domain ... Gel Mobility Shift Assays Gel mobility shift assays were used to evaluate the binding interaction between HuR and RNA In these assays, the amount of RNA-bound HuR present in solution is assessed...
... trạng TB nuôi cấy Cây tái sinh Truyền lại cho hệ sau Methionine sulfximine (pseudomonas syringe) Kháng Thuốc + lại độc tốlá bệnh + + Tabtoxin tinh khiết (pseudomonas syringe pv tabaci) Kháng Thuốc ... Kháng amino acid đồng đẳng amino acid Kháng bệnh Kháng thuốc diệt cỏ Chống chịu với stress môi trường Kháng kháng sinh Kháng đồng đẳng base DNA Một số ví dụ thành công chọn lọc invitro đột ... chia làm ba nhóm: sinh lý, di truyền, hoá sinh Hai nguyên nhân gây biến dị dòng soma là: tính không đồng di truyền tế bào soma mẫu cấy ban đầu tác động yếu tố trình nuôi cấy invitro 3.1 Sự đa dạng...
... pyridoxine, thiamin, glycine, fonic acid v biotin + Casein hydrolysate, D-Ca- pantothenate, choline chloride, cysteine, malic acid, ascorbic acid, adenine sulfate, riboflavin v glutamine: cú ... dinh dng ph bin: Mui khoỏng: theo White (1943), Heller (1953), Murashige v Skoog (1962) Cht hu c: ng sarcaroza Vitamin: B, B6, inositol, nicotin axit Hoocmon: auxin (IAA, IBA, NAA), Xytokinin ... iu tit sinh trng hoc mụi trng cú cha xytokinin nng thp thỡ phụi phỏt trin thnh cõy hon chnh Bc 5: Thớch ng cõy invitro ngoi iu kin t nhiờn a cõy t ng nghim ngoi m vi t l sng cao, cõy sinh trng...
... the loading of pravastatin into erythrocytes was directly proportional with increase of drug concentration in the incubation medium, in the 2-10 mg/ml concentration range This finding is in concurrence ... pravastatin However, the relative impacts of different invitro findings on the overall in vivo drug delivery efficacy of these cellular carriers remain to be evaluated during the future in vivo ... statins including inhibition of the metabolism [5], administration of statins with certain juices [6] or inhibition of Pgp [3] Unfortunately these strategies are frequently associated with increase...
... phosphate-buffered saline (PBS), and resuspended at × 106 cells /mL in Annexin-V binding buffer The supernatant (100 μL/tube) was incubated with μL of Annexin-V-FITC (Invitrogen, USA) and μL of PI (Invitrogen, ... (GIBCO, USA) containing 10% heat-inactivated fetal bovine serum (Nuoding, China), and the HT-29 cells were grown in DMEM medium (GIBCO, USA) also containing 10% heat-inactivated fetal bovine serum All ... doxorubicin-loaded, long-circulating liposomes modified with cancer-specific monoclonal antibody Clin Cancer Res 2009; 15:1973-80 Lin HY, Lansing L, Merillon JM, et al Integrin alphavbeta3 contains...
... nuôi cấy tỷ lệ Auxin/ Cytokinin định phân chia tế bào, phân hóa chồi từ mô sẹo, tạo phôi vô tính - Cytokinin gồm có kinetin, BAP, zeatin, 2iP + Kinetin (6 - fafurolaminoprrine) hình thành phát ... chất auxin/ cytokinin môi trường phát sinh quan (rễ chồi) mô sẹo thuốc Khi tỷ lệ auxin/ cytokinin như: IAA/ kinetin < mô có xu hướng hình thành chồi Khi tỷ lệ auxin/ cytokinin > mô sẹo có khuynh ... lượng protein rõ ràng cao hẳn Bên cạnh hàm lượng lớn protein lipit, hạt đậu tương giàu nguồn sinh tố muối khoáng chứa nhiều loại axit amin có axit amin không thay như: Arginin, Histidin, Lysin, Triptophan...
... protocorms with 2-3 cm in length are the most suitable for invitro rapid multiplication in the most suitable culture medium Knud* that supplemented with 0,5 mg/l BAP + 0,3 mg/l Kinetin + 0,3 mg/l NAA ... results of research on invitro rapid multiplication of protocorms and shoots of Jewel Orchid species Anoectochilus roxburghii (Wall.) Lindl Knud* medium is the most suitable for invitro rapid multiplication ... Effect of Environmental Conditions on InVitro and Ex Vitro Growth of Jewel Orchid Anoectochilus formosanus Hayata, Thesis for the Degree of Doctor of Philosophy in Agriculture, The Graduate School...
... quinon, alcaloid, tinh dầu, acid hữu cơ, phytosterol - Dịch chiết cồn: quinon, flavonoid, alcaloid, acid hữu cơ, sterol, đường khử, tanin, anthocynosid - Dịch chiết nước acid: alcaloid, tanin, ... Gelatin - - - Acid hữu -Tinh thể Na CO -Xanh Bromothymol + + + + + + Đường khử -Fehling A- B + + - Anthocyanosid - HCl; KOH - - - Tinh dầu -Bay - - - 10 Caroten - H SO 10% - - - 11 Saponin + ... 12h, lọc, sấy Tinh thể P lần Tinh thể P lần Hình 3.4: Sơ đồ quy trình chiết xuất, tinh chế chất P2 sau sắc ký lớp chế hoá 3.4 Nhận dạng chất P2 chiết - Thể chất: tinh thể P2 tinh thể hình kim,...
... 2003 Influence of reproductive status on invitro oocyte matuation in dogs Theriogenlogy 60: 59 – 66 [28] Sara C., Luvoni G.C., Elisa A and Debora M., 2005 Factor involved in vivo and invitro ... noãn bò trưởng thành invitro Năm 1988, Lu cs cho đời bê từ kỹ thuật chín noãn thụ tinh invitro Năm 1996, Eppig O’Brien cho đời chuột sau dùng kỹ thuật IVM, thụ tinh invitro chuyển phôi vào tử ... invitro Năm 1983, Minato Toyoda (Nhật) Schroeder Eppig (Mỹ) cho noãn chuột trưởng thành invitro có khả tạo phôi thụ tinh Năm 1983, Lenz cs cho 39oC nhiệt độ tối ưu để noãn bò trưởng thành in...