binding gtpase assay by thin layer chromatography
Applied thin layer chromatography best practice and avoidance of mistakes 2nd ed e hahn deinstrop (wiley, 2007) WW
... components The term thin- layer chromatography , introduced by E Stahl in 1956, means a chromatographic separation process in which the stationary phase consists of a thin layer applied to a solid ... pharmacopoeia is indicated by the suffix USP, the British by BP, and the European by CRS The possible use of a PS is specified by the relevant institutions, and is terminated by a change in the LOT ... sulfonic acids, carboxylic acids, xanthines DIOL-modified layer Amino-modified layer NH2 Anion exchange, normal phase and reversed phase chromatography RP layers: RP-2, RP-8, RP-18 Silica gel...
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THIN LAYER CHROMATOGRAPHY GAS CHROMATOGRAPHY
... I THIN- LAYER CHROMATOGRAPHY (TLC) A LÝ THUYẾT Sắc ký lớp mỏng (TLC) kĩ thuật quan trọng cho phân tách nhanh ... mỏng để khô, hiển thị vết với Iot, mô tả B.1 Làm bảng phác thảo đĩa bạn ghi kết vào sổ tay II.GAS CHROMATOGRAPHY A.Lý Thuyết Sắc ký khí công cụ hữu ích cho phân tách phân tích hợp chất hữu bay mà...
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SẮC ký lớp MỎNG TLC Thin layer chromatography
... đƣợc phiến kính triển khai chiều thứ II hay chạy lần II D KỸ THUẬT SẮC KÝ CHẾ HÓA (PREPARATIVE CHROMATOGRAPHY) Tách chất với lƣợng nhỏ trƣờng hợp tách phƣơng pháp khác Dùng kỹ thuật sắc ... KỸ THUẬT SRS (SEPARTION-REACTION-SEPARATION) 22 D KỸ THUẬT SẮC KÝ CHẾ HÓA (PREPARATIVE CHROMATOGRAPHY) 22 IX- ỨNG DỤNG TLC TRONG PHÂN TÍCH DƢỢC 23 ĐỊNH TÍNH VÀ THỬ TINH...
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High-Performance Thin-Layer Chromatography(HPTLC)
... of unresolved analytes Liquid Chromatography Thin- Layer Chromatography (LC–TLC) The most general interface for coupling column liquid chromatography to thinlayer chromatography (LC–TLC) is based ... liquid chromatography mass spectrometry (LC–MS) than to either quantify or identify separated bands by thin layer chromatography mass spectrometry (TLC–MS) High-Performance Thin- Layer Chromatography Mass ... processing of images obtained by thin- layer chromatography J Chromatogr A, 2008, 1188, (2), 295–300 Stahl E (1965) Thin- Layer Chromatography – A Laboratory Handbook, published by SpringerVerlag, Berlin,...
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Determination of EDTA in Water Samples by SPE-Gas Chromatography/Mass Spectrometry
... Milli-Q water was used in all experiments Gas chromatography/ mass spectrometry analysis Gas chromatography was carried out by using HP6890 series gas chromatography system (Hewlett Packard, Wilmington, ... Okumura, 1995), and liquid chromatography/ tandem spetrometry (Quintana and Reemtsma, 2007) To simplify Japan’s recommended method of EDTA determination by using gas chromatography- mass spectrometry, ... Technology, Vol 8, No.4, 2010 chromatography (Nowack et al., 1996; Kemmei et al., 2009), ion chromatography/ mass spectrometry (Bauer et al., 1999; Knepper et al., 2005), gas chromatography/ mass spetrometry...
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Tài liệu Báo cáo khoa học: The resident endoplasmic reticulum protein, BAP31, associates with c-actin and myosin B heavy chain Analysis by capillary liquid chromatography microelectrospray tandem MS ppt
... the time-course for activation of caspases, as assayed by the processing of procaspases and 3, by the measurement of total DEVDase activity, and by the cleavage of the caspase targets poly(ADP-ribosyl) ... until use was achieved by manually excluding tandem mass spectra of poor quality, by restricting the database to the proteins that were identified in a first pass approach, and by changing the SEQUEST ... accession P02571) by LC-lESI-MS/MS All amino acids are in single-letter code The peptides unequivocally identified by spectral matching of the tandem mass spectra with the sequence database by SEQUEST...
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Tài liệu Binding Protein Edited by Kotb Abdelmohsen pot
... evidence to suggest that retinol binding is positively regulated by CKII phosphorylation at a conserved site located near the binding pocket associated with retinol binding [15] Previous research ... Retinol -Binding Protein (HpFAR-1) from Heligmosomoides polygyrus J Nemat 41(3):228-233 Relative fluorescence intensity (arbitrary units) Figure Binding of DAUDA by Hp-FAR-1 indicated by a substantial ... Secreted Fatty Acid and Retinol -Binding Protein (HpFAR-1) from Heligmosomoides polygyrus J Nemat 41(3):228-233 Figure Binding of retinol by Hp-FAR-1 indicated by a substantial increase in fluorescence...
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Báo cáo khoa học: Interference with the citrulline-based nitric oxide synthase assay by argininosuccinate lyase activity in Arabidopsis extracts docx
... plants comes from commercially available ‘NOS assay kits’ (citrulline-based assays) that measure the conversion of arginine to citrulline using ion exchange chromatography [44] Radiolabeled arginine ... protein extracts from Arabidopsis leaves By employing the citrulline-based NOS assay, an arginine-dependent activity was discovered that was strongly stimulated by an extract of low molecular weight ... fumarate by argininosuccinate lyase (ASL; EC 4.3.2.1; Fig 5) Argininosuccinate is normally made from citrulline and aspartate by argininosuccinate synthetase, but it can also be produced by ASL...
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Báo cáo khoa học: Characterization of HbpR binding by site-directed mutagenesis of its DNA-binding site and by deletion of the effector domain pot
... ⁄ D-1) is indicated by small black pillars HbpR is depicted schematically as a hexamer Enlarged is the operator region bound by HbpR within the hbpC promoter The arrows within the sequence point ... 1–7, Fig 2) All mutant binding sites were then tested for affinity binding in electrophoretic mobility shift assays (EMSAs) with increasing concentrations of calmodulin binding protein (CBP)–HbpR ... of the binding region was not influenced by the change from four AT to four or six GC, was shown by mutants and (each with 3· GC and five mutations) Both mutants and were bound equally well by HbpR...
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Báo cáo khoa học: Pherokine-2 and -3 Two Drosophila molecules related to pheromone/odor-binding proteins induced by viral and bacterial infections ppt
... was analyzed by HPLC and the molecule induced by the viral infection was detected by MALDI-TOF MS (Fig 2B) The molecule was purified to homogeneity by gel permeation and reversed-phase chromatography ... pathway) mutant flies were infected by septic injury, and expression of phk genes was analyzed by Northern blot (C) phk-3 Expression is upregulated by LPS (+) and repressed by the molting hormone ecdysone ... describe in this report two new molecules which are induced by septic injury Phk-2 is induced by DCV infection, whereas Phk-3 is induced by bacterial challenge The third member of this family in...
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Báo cáo Y học: Holliday junction binding and processing by the RuvA protein of Mycoplasma pneumoniae ppt
... J Biochem 269) 1529 Fig Holliday junction binding by MpRuvA (A) Gel retardation assay showing the formation of complexes I and II with junction J11 Binding mixtures contained 0.2 ng 32P-labelled ... purified as described previously [32,33] Protein concentrations were estimated by a modified Bradford assay using a Bio-Rad assay kit and bovine serum albumin as standard Amounts of RuvA, RuvB and RuvC ... amount of MpRuvA binding to duplex DNA compared to EcRuvA, as indicated by the response (Figs 3C–E) MpRuvA also formed a complex with a short 24 bp duplex that was not bound detectably by EcRuvA (data...
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Báo cáo Y học: The role of arginine residues in substrate binding and catalysis by deacetoxycephalosporin C synthase potx
... Purification and assay of DAOCS mutants Wild-type DAOCS and the Arg160 and 162 mutants were purified to 85% homogeneity (by SDS/PAGE analysis) from 25 g of frozen recombinant E coli cells by anionexchange ... (observed)] Activity assays Radioactive 2-oxoglutarate conversion assays were conducted as previously described [17], using 0.1 mM penicillin N, 10 mM penicillin G or water as substrates Assays based ... system [1] Assay mixtures containing penicillin G were purified by HPLC with a Hypersil C4 column (250 · 4.6 mm) using mL of 25 mM NH4HCO3 in 15% (v/v) methanol at mLÆmin)1, followed by a gradient...
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kowalska - preparative layer chromatography (crc, 2003)
... Handbook of Thin- Layer Chromatography, edited by Joseph Sherma and Bernard Fried 56 Gas–Liquid–Solid Chromatography, V G Berezkin 57 Complexation Chromatography, edited by D Cagniant 58 Liquid Chromatography Mass ... Liquid Chromatography, edited by Ira S Krull 35 Thin- Layer Chromatography: Techniques and Applications, Second Edition, Revised and Expanded, Bernard Fried and Joseph Sherma 36 Quantitative Thin- Layer ... Applications of Glass Capillary Gas Chromatography, edited by Walter G Jennings Steroid Analysis by HPLC: Recent Applications, edited by Marie P Kautsky Thin- Layer Chromatography: Techniques and Applications,...
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Báo cáo hóa học: " Improved characteristics of near-band-edge and deep-level emissions from ZnO nanorod arrays by atomic-layer-deposited Al2O3 and ZnO shell layers" potx
... Tsai FY: Enhancement in the efficiency of light emission from silicon by a thin Al2O3 surface-passivating layer grown by atomic layer deposition at low temperature J Appl Phys 2007, 101:033130 36 ... Structure and stimulated emission of a high-quality zinc oxide epilayer grown by atomic layer deposition on the sapphire substrate Thin Solid Films 2010, 519:536-540 Page of 34 Shih YT, Wu MK, Li ... 180°C The Al O shell layers were 2, 5, and 10 nm in thickness The ALD condition of ZnO shell layers was the same as that of the ZnO seed layer The thicknesses of ZnO shell layers were 5, 10, and...
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Báo cáo hóa học: " Multi-Directional Growth of Aligned Carbon Nanotubes Over Catalyst Film Prepared by Atomic Layer Deposition" doc
... gaseous by- products, monolayer iron compounds were adsorbed As ozone oxidized the iron compounds, monolayer iron oxides were obtained during a cycle After a purge to evacuate ozone and byproducts, ... on CNTs by ALD for the application in proton-exchange membrane fuel cells [24]; ALD was also employed to grow coaxial thin films of Al2O3 [25], V2O5, TiO2, HfO2, [26, 27], and Al2O3/W bilayers ... substrate should be developed Recently, atomic layer deposition (ALD) has become an important way to fabricate thin film on various substrates It is a thin film growth method based on sequential,...
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Báo cáo hóa học: " Adaptive QoS Routing by Cross-Layer Cooperation in Ad Hoc Networks" pptx
... a link state is dominated by two factors: one is the lower -layer status, such as node mobility and radio channel characteristics; the other is the higher -layer traffic By exchanging neighbor’s local ... information in the network model, a link’s lower -layer characteristics can be moved up to a higher layer Combining these lower -layer parameters with the higher -layer parameters in a link model, we are ... This packet delivery ratio is obtained by dividing the number of data packets correctly received by the destination by the number of data packets originated by the source Obviously, mobility increases...
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Báo cáo y học: "BoCaTFBS: a boosted cascade learner to refine the binding sites suggested by ChIP-chip experiments" pot
... explained just by chance, to retrieve these binding sites The putative binding sites on chromosome 22 were retrieved by Clover in this way There are 173 Sp1 binding sites, 627 cMyc binding sites, ... locations of binding regions, but they produce a massive amount of nonbinding information We use this massive nonbinding information and the known binding information for prediction of the binding ... Therefore, there are 6,869,066 cMyc nonbinding sites, 6,907,805 Sp1 nonbinding sites, and 6,913,291 P53 nonbinding sites Both the binding sites and nonbinding sites were used for the training...
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Mechanism of action and binding mode revealed by the crystal structures of key enzymes in plants sugar metalbolic pathway
... Molecular surface of SPS showing the distinct two domains separated by a large substrate binding cleft (b) Close-up view of the F6P binding site (c) 46 2.2 2.3 2.4 2.5 2.6 2.7 2.14 2.15 2.16 2.17 ... S6P binding site Superimposition of F6P-SPS and S6P-SPS complexes Ten docked models of UDP interacting with the binding residues of H orenii SPS Ten docked models of ADP interacting with the binding ... produced by the light-independent pathway of photosynthesis, when carbon dioxide is reduced by reacting with ribulose 1,5-bisphosphate to form two molecules of glycerate 3-phosphate By using...
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