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Tài liệu Báo cáo khoa học: Molecular characterization of Arabidopsis thaliana PUF proteins – binding specificity and target candidates doc
Ngày tải lên :
18/02/2014, 06:20
... human and
mouse possess two; and Drosophila and Dyctiostelium
have only one member [5]. Recently, new studies have
revealed the presence of ten, two and one homologs
in Trypanosome, Plasmodium and ... UGUR and AUA sequence sepa-
rated by two nucleotides, whereas C. elegans PUF-8,
Drosophila and human Pumilio and yeast Puf3 recog-
AB
CD
Fig. 6. Analysis of binding afnity between APUM-2 and the ... binding speci city
observed between APUM-2 and NRE can be extended
to the remaining group I proteins, we tested the interac-
tion of APUM-1, APUM-3, APUM-4, APUM-5 and
APUM-6 with wild-type and mutant...
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Tài liệu Báo cáo khoa học: Substrate specificity and inhibition of brassinin hydrolases, detoxifying enzymes from the plant pathogens Leptosphaeria maculans and Alternaria brassicicola ppt
Ngày tải lên :
18/02/2014, 14:20
... 2-mercaptoethanol and dithiothreitol. For example,
1mm dithiothreitol caused 72% and 85% inhibition of
BHLmL2 and BHAb, respectively, while 1 mm 2-mer-
captoethanol caused 85% and 97% inhibition of
BHLmL2 and ... Substrate speci city and inhibition of brassinin
hydrolases, detoxifying enzymes from the plant pathogens
Leptosphaeria maculans and Alternaria brassicicola
M. Soledade C. Pedras, Zoran Minic and Vijay ... oilseeds rapeseed and canola (Bras-
sica napus and Brassica rapa) and vegetables such ase
cabbage (Brassica oleraceae var. capitata), cauliflower
(B. oleraceae var. botrytis) and broccoli (B. oleraceae
Keywords
brassinin;...
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Tài liệu Báo cáo khoa học: A knowledge-based potential function predicts the specificity and relative binding energy of RNA-binding proteins ppt
Ngày tải lên :
18/02/2014, 16:20
... structure.
Some RRM and KH domains in complex with single
strand DNA (PDB codes: 2UP1, 1WTB, 1X0F, 1ZZI and
1ZZJ) were also included in the test set because recognition
of single stranded RNA and DNA are ... structures
(e.g. 1CVJ_1 and 1CVJ_2 represent the first and sec-
ond Poly A binding protein domain of structure
1CVJ, respectively), and the two domains were con-
sidered structurally and thermodynamically ... approaches because of the
demanding computational requirements.
This application relies on a specific structural
model of RNA recognition by RRM and KH
Table 1. Native Z-scores and score–rmsd correlation...
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Tài liệu Báo cáo khoa học: Structural insights into the substrate specificity and activity of ervatamins, the papain-like cysteine proteases from a tropical plant, Ervatamia coronaria ppt
Ngày tải lên :
18/02/2014, 16:20
... papain and actinidin, and on amino
acid sequence information for cathepsins B and H, and
stem bromelain. J Mol Biol 182, 317–329.
11 Carter CE, Marriage H & Goodenough PW (2000)
Mutagenesis and ... stability, substrate
speci city, and activity. The stability aspect has been
extensively discussed in our earlier papers [27,35], and
here we address the issue of substrate speci city and
activity in greater ... temperature and pH, but also for variations in activity and
speci city among themselves and among other members of the family. Here
we report the crystal structures of ervatamin-A and ervatamin-C,...
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Tài liệu Báo cáo khoa học: The role in the substrate specificity and catalysis of residues forming the substrate aglycone-binding site of a b-glycosidase docx
Ngày tải lên :
18/02/2014, 17:20
... Behaviour and Analy-
sis of Rapid Equilibrium and Steady-State Enzyme
Systems. John Wiley and Sons, New York, NY.
Molecular basis for speci city of a b-glycosidase L. M. F. Mendoncáa and S. R. ... importance in understanding the
physiological role of b-glycosidases and in designing
inhibitors.
In addition, another important issue in understand-
ing the aglycone speci city is the contribution ... stabilization
of ES
à
and the interdependence between the binding of
aglycone and the positioning of glycone in subsite )1
are not known.
In this study, the role in the substrate speci city
and catalysis...
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Báo cáo khoa học: Substrate specificity and transport mode of the proton-dependent amino acid transporter mPAT2 potx
Ngày tải lên :
07/03/2014, 16:20
... not only t he amino acids glycine, alanine, serine
and proline but also osmolytes such as sarcosine and
betaine, and the
D
-enantiomers of serine and alanine. The
apparent affinities of PAT1 substrates ... potential and extracellular p H have quite
diverse effects on the kinetics of transport by PAT2.
Maximal transport velocity was only moderately affected by
both membrane potential and extracellular ... K
m
values
of glycine (A) and
L
-alanine (B) at t he extracellular p H 8.5 (h), p H 7.5
(n), pH 6.5 (e), and pH 5.5 (s) in PAT2 expressing oocytes. (C)
Apparent K
m
values of glycine (h )and
L
-alanine (s)...
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Báo cáo khoa học: pH dependence, substrate specificity and inhibition of human kynurenine aminotransferase I ppt
Ngày tải lên :
07/03/2014, 16:20
... conditions, indicates indo-3-
pyruvate and cysteine a s efficient inhibitors for hKAT-I, and
also provides biochemical information about the s ubstrate
speci city and cosubstrate inhibition of the enzyme. ... a ltered
kinetics; l ower initial velocity and K
m
for both kynurenine
and pyruvate [22]. This mutation m ay explain the enhanced
sensitivity to glutamate and nicotine seen in spontaneously
hypertensive ... i.e. wide
a-k eto acid speci city, but high activity with a-KMB and
glyoxylate, strong substrate i nhibition with ph enylpyruvate
and poor affinity toward alanine and pyruvate [30,41].
In summary,...
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Báo cáo khoa học: Relation between domain evolution, specificity, and taxonomy of the a-amylase family members containing a C-terminal starch-binding domain pot
Ngày tải lên :
08/03/2014, 08:20
... constructed for domains
A, B, C and E and compared with the Ôcomplete-sequence
treeÕ. The trees for domains A and B and the complete-
sequence tree were very similar and contain two main
groups of ... third b-strand and third a-helix and varies greatly in
length, sequence and tertiary structure [20,24]. Domain C,
which immediately succeeds the catalytic barrel, is essen-
tially a b-sandwich structure ... many theoretical and experimental
results on sequence and structure similarities between
amylases and CGTases [19,20,22,26,30,94,116–118], their
phylogenies [20,94,106,119–121], and a novel SBD...
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Báo cáo khoa học: LmbE proteins from Bacillus cereus are de-N-acetylases with broad substrate specificity and are highly similar to proteins in Bacillus anthracis pot
Ngày tải lên :
15/03/2014, 11:20
... (Heraklion,
Greece) and New England Biolabs GmbH. The instruments
Fluostar Galaxy and Mastercycler Gradient (for PCR and
RT-PCR) were purchased from BMG Labtechnologies
GmbH (Offenburg, Germany) and Eppendorf ... (EC
3.5.1 ) and BC3461 were biochemically characterized. The enzymes have
different molecular masses, pH and temperature optima and broad sub-
strate speci city, de-N-acetylating GlcNAc and N-acetylchito-oligomers
(GlcNAc
2
, ... 140 and 42 using Xtal-
View ⁄ Xfit [42] and ⁄ or MIFit (Rigaku Americas Corp.). All
water and acetate atoms were removed. Hydrogen atoms
were added to the crystal structure, and the substrates and
atomic...
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Báo cáo khoa học: Hybrid reuteransucrase enzymes reveal regions important for glucosidic linkage specificity and the transglucosylation / hydrolysis ratio pptx
Ngày tải lên :
16/03/2014, 04:20
... (confirmed by DNA nucleotide sequencing),
both p15-GTFA-dN-RS and p15-GTFO-dN-RS were
digested with XbaI and SalI, SalI and SacI, and SacI and
KpnI, and corresponding fragments were exchanged, yielding
the ... p15-GTFA-dN and p15-GTFO-dN were partially
digested with BamHI and KpnI to exchange the N- and
C-termini of the (N-terminally truncated) gtfA and gtfO
genes, yielding constructs p15-GTF-AO-dN and p15-
GTFOA-dN ... [1–5]. GTFA and GTFO show 68%
sequence identity, and synthesize reuterans with
approximately 50% and 70% a-(1fi4) glucosidic link-
ages, respectively, plus a-(1fi6) linkages ( 50% and
30%, respectively)....
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Báo cáo khoa học: Kinetic studies and molecular modelling attribute a crucial role in the specificity and stereoselectivity of penicillin acylase to the pair ArgA145-ArgB263 pdf
Ngày tải lên :
16/03/2014, 16:20
... both steric and electronic factors.
The structures listed in Table 1 are of different size and
hydrophobicity, without and with electronegative substitu-
ents of different orientation, and the majority ... speci city is more
pronounced and the synthetic capacity is, respectively,
increased. In the latter system PA behaves as a typical
transferase [14,15]. The nucleophile or S
1
 [16] specicity ... facilitate
rapid and reliable kinetic measurements.
The acyl speci city of PA is restricted to aromatic
molecules and has been investigated mainly with substrates
containing phenylacetyl, phenylglycyl, mandelyl,...
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Báo cáo Y học: The role of hydrophobic active-site residues in substrate specificity and acyl transfer activity of penicillin acylase pdf
Ngày tải lên :
17/03/2014, 23:20
... speci city
and acyl transfer activity of penicillin acylase
Wynand B. L. Alkema, Anne-Jan Dijkhuis, Erik de Vries and Dick B. Janssen
Department of Biochemistry, Groningen Biomolecular Sciences and ... volume of the acyl binding pocket
and thereby alter the binding mode and affinity of the
enzyme for PAA and derivatives thereof, while maintaining
the hydrophobicity of the binding site.
To test ... The subscripts AD and P refer to the acyl donor and
product, respectively. The speci city (k
cat
/K
m
)ofPAforthe
produced antibiotic is in general 10-fold higher than the
speci city for the corresponding...
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Báo cáo khoa học: Substrate specificity and excision kinetics of natural polymorphic variants and phosphomimetic mutants of human 8-oxoguanine-DNA glycosylase pot
Ngày tải lên :
23/03/2014, 05:22
... on the activity and
opposite-base speci city of OGG1, we determined the
kinetic constants k
cat
and K
m
for the cleavage of
8-oxoGua:Cyt and 8-oxoGua:Ade substrates by wild-
type and mutant OGG1 ... FapyGua and 8-oxoGua were 1.25–7.67 lm and 0.56–
3.58 lm, respectively. The other set was incubated with an
enzyme and analyzed by GC ⁄ MS as described above. The
kinetic constants and standard ... bulk
and charge of phosphoserine [40].
In this article, we analyze the activity, substrate
speci city and kinetics of two naturally occurring poly-
morphic variants of OGG1, OGG1-A288V and
OGG1-D322N,...
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Báo cáo khoa học: Increased amylosucrase activity and specificity, and identification of regions important for activity, specificity and stability through molecular evolution doc
Ngày tải lên :
23/03/2014, 11:20
... compilation ª 2006 FEBS
Increased amylosucrase activity and speci city, and
identification of regions important for activity, speci city
and stability through molecular evolution
Bart A. van der ... resulting in a high affinity and low V
max
at
low sucrose concentrations (V
max1
and Km
1
) and low
affinity and high(er) V
max
at high sucrose concentra-
tions (V
max2
and Km
2
) [13]. In Table ... as
b strand 7, connecting to the important Asp393 resi-
due, and the region close to the salt bridge and the
secondary sucrose-binding site SB2. Other regions are
involved in speci city and thermostability,...
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