band s from gel electrophoresis in vivo complex sample

Báo cáo Y học: A nonphosphorylated 14-3-3 binding motif on exoenzyme S that is functional in vivo pot

Ngày tải lên : 31/03/2014, 08:20

... Commassie blue stained SDS/PAGE, shows purified GST-fusion proteins purified from Escherichia coli used in this study Lanes correspond to schematic representations of the constructs illustrated in ... three washes in lysis buffer, samples were boiled in SDS/PAGE sample buffer Protein samples were loaded onto a single wide lane of an SDS/ polyacrylamide gel After electrophoresis the separated ... eukaryotic cells by a genetically defined Y pseudotuberculosis strain and also by several different Pseudomonas aeruginosa strains [34,35] The Yersinia strain expresses and translocates ExoS protein with...

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Báo cáo khóa học: Characterization of presenilin complexes from mouse and human brain using Blue Native gel electrophoresis reveals high expression in embryonic brain and minimal change in complex mobility with pathogenic presenilin mutations pptx

Ngày tải lên : 16/03/2014, 16:20

... levels were found in these tissues as shown in Fig 5C using Western analysis after standard SDS gel electrophoresis We next examined cases of early onset familial AD with pathogenic PS1 missense ... twice) PS1 expression and NCT levels for some of these early onset cases is shown in Fig 5D SY5Y cells stably transfected with PS1 cDNA constructs were used to examine effects of over-expression ... Tris/tricine gels with reducing Ó FEBS 2003 Analysis of mouse and human brain presenilin complex (Eur J Biochem 271) 381 Fig PEN-2 and APH-1 expression in mouse and human tissues and detection in PS complex...

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Báo cáo y học: "Psoriasin, one of several new proteins identified in nasal lavage fluid from allergic and non-allergic individuals using 2-dimensional gel electrophoresis and mass spectrometry" potx

Ngày tải lên : 12/08/2014, 18:22

... protein Prolactin-inducible protein Prolactin-inducible protein Prolactin-inducible protein Cystatin S Cystatin S Cystatin SN Hemoglobin beta chain Hemoglobin beta chain Intersectin 2, (splice isoform ... Albumin Albumin Albumin Albumin Albumin Albumin Albumin Albumin Albumin Albumin Albumin Albumin Albumin Albumin Albumin Albumin Albumin Albumin Albumin Albumin Albumin Albumin Albumin Prolactin-inducible ... western blot Statistical analysis All values were expressed as mean values ± SEM Statistical analysis of the protein expression was performed in Ludesi 2D interpreter (Ludesi AB) using one-way...

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Tài liệu EFFECTS OF AIR POLLUTION FROM A NICKEL-COPPER INDUSTRIAL COMPLEX ON BOREAL FOREST VEGETATION IN THE JOINT RUSSIAN-NORWEGIAN-FINNISH BORDER AREA ppt

Ngày tải lên : 17/02/2014, 22:20

... scots pine Birch, scots pine Birch Birch Birch Birch Birch Birch Birch Birch, scots pine scots pine scots pine scots pine scots pine scots pine scots pine scots pine Ground Deposition humus vegetation ... scots pine scots pine scots pine scots pine scots pine scots pine scots pine average stand age in 2004 (years) 60 45 56 50 67 ~200 200+ 200–300 235 185 ~200 188 192 191 vegetation type (Påhlsson ... were assessed, and the crown density was estimated in 1% classes Mechanical damage arising from snow break, wiping etc was excluded Crown colour was estimated using the ICP Forest classes (http://www...

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Tài liệu Báo cáo khoa học: The endogenous retinoid metabolite S-4-oxo-9-cis-13,14-dihydro-retinoic acid activates retinoic acid receptor signalling both in vitro and in vivo pdf

Ngày tải lên : 18/02/2014, 08:20

... analysis and days for analysis of wing patterns Analysis of wing patterns and dissection of limb bud tissue After days of incubation, embryos were sacrificed, washed three times in water, fixed in ... digits which are proximally fused are shown in italic Discussion The number of identified endogenous retinoid receptor ligands in plasma and ⁄ or soft tissues of various species, including humans, ... nuclear retinoid receptors and regulate gene transcription both in vitro and in vivo Retinoids (vitamin A and its analogues) play essential roles in several physiological processes, such as embryonic...

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Báo cáo khoa học: Activator-binding domains of the SWI ⁄ SNF chromatin remodeling complex characterized in vitro are required for its recruitment to promoters in vivo pot

Ngày tải lên : 07/03/2014, 00:20

... Thus, the FRET and cross-linking approaches appear to cross-validate each other A photo-crosslinking approach has identiﬁed the Swi1, Snf5 and Swi2 ⁄ Snf2 subunits of the SWI ⁄ SNF complex as ... is severely reduced in a strain lacking both the Swi1 and Snf5 activator-binding domains Recruitment of the SWI ⁄ SNF complex via activator interactions with these activator-binding domains is ... non-inducing sugars such as rafﬁnose, the Gal4 activator is bound to its DNA binding sites in target genes but its activity is repressed by its association with the Gal80 repressor protein Upon addition...

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Báo cáo khoa học: Site speciﬁcity of yeast histone acetyltransferase B complex in vivo pot

Ngày tải lên : 07/03/2014, 05:20

... This study This study [19] [19] [19] M Grunstein M Grunstein This This This This This This This This study study study study study study study study tion approximately 10 times higher than suggested ... out the possibility that histone H3 proteolysis is also occurring in yeast soluble extracts in our experiments, but its presence in input and unbound fractions argues against this possibility ... 2008 FEBS A Poveda and R Sendra Yeast histone acetyltransferase B complex Table Yeast strains used in this study Strain Relevant genotype Source W303-1a RS1263 BQS1154 MCY730 BQS179 YSTT11 YSTT49...

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Báo cáo Y học: In vivo activation of plasma membrane H+-ATPase hydrolytic activity by complex lipid-bound unsaturated fatty acids in Ustilago maydis docx

Ngày tải lên : 08/03/2014, 22:20

... ratio displayed the characteristic DISCUSSION The use of sterol biosynthesis inhibitors is a usual way of evaluating the physiological effects that lipids, in particular Table Kinetic parameters of ... use of mutants and inhibitors acting on the same biosynthetic points has proved to be a useful way of distinguishing the in uence of direct effects, i.e sterol alteration, and indirect effects ... lipid intermixing The reaction was terminated by adding the stopping reagent used for phosphate determination Consumption of substrate by the H+-ATPase was less than 15% under any conditions Kinetic...

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Báo cáo khoa học: Oxidative stress induces a reversible ﬂux of cysteine from tissues to blood in vivo in the rat pptx

Ngày tải lên : 16/03/2014, 02:20

... and organs Disulfide forms comprise low-molecular-mass disulfides [LMM-SS: CySS, cystinylglycine (CySSGly), homocystine (HcySS) and GSSG] and mixed disulfides between protein sulfhydryl groups and ... experiments, we observed a significant and progressive increase in total plasma cysteine, mainly CySS and CySSP, after diamide infusion, corresponding to the cysteine concentration decrease within RBCs ... nor GSSG, can be taken up by most cells (also including RBCs) Some cell types (excluding RBCs) also possess a transport system for uptake of CySS Extracellular GSH can be converted into CysGly...

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Báo cáo khoa học: Inhibition of the mitochondrial calcium uniporter by the oxo-bridged dinuclear ruthenium amine complex (Ru360) prevents from irreversible injury in postischemic rat heart docx

Ngày tải lên : 16/03/2014, 22:20

... Radioactivity retained in the filters was measured in a scintillation counter and nonspecific binding was determined with 20 lm ryanodine Statistics The results are expressed as mean ± SE Significance ... and release of solutes of < 1500 Da across the inner membrane, through a pore sensitive to the immunosuppressant, cyclosporin A (CsA) [6,7] Increase of [Ca2+]m is a specific and almost absolute ... currents, as determined in SR vesicles or in isolated myocytes [15] To gain insight into the contribution of the mitochondrial uniporter to myocardial injury during I ⁄ R in isolated hearts, we...

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Báo cáo khoa học: Crystal structure of the halotolerant c-glutamyltranspeptidase from Bacillus subtilis in complex with glutamate reveals a unique architecture of the solvent-exposed catalytic pocket docx

Ngày tải lên : 22/03/2014, 21:20

... GGTs with known structures, it is assumed that B subtilis GGT undergoes no signiﬁcant structural change during maturation In B subtilis GGT, the segment consisting of residues 391–395 undergoes ... [13] We were interested in Bacillus subtilis GGT because it lacks the sequences corresponding to the lid-loop, and instead has extra residues at the C-terminus of the L-subunit, whereas the catalytic ... properties was assessed by solving the Poisson– Boltzmann equation At high salt concentrations, notably different results were observed in the electrostatic surface potentials between the two GGTs; in...

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Báo cáo khoa học: LRRK2 in Parkinson’s disease: in vivo models and approaches for understanding pathogenic roles pdf

Ngày tải lên : 23/03/2014, 04:20

... mechanisms of many disease processes and will continue to impact our understanding of disease pathogenesis This minireview will examine the previous studies of genes for ROCO or LRRK2 homologs in slime ... known as frequent ‘contaminants’ in the process of searching binding proteins, the in vivo evidence for the relationship between ROCO proteins and cytoskeletons in D discoideum suggests a need ... enhanced sensitivity to endoplasmic reticulum stress induced by tunicamycin, a speciﬁc inhibitor for N-linked glycosylation Interestingly, this enhanced sensitivity is suppressed in mutant worms with...

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Báo cáo khoa học: X-ray structure of glucose/galactose receptor from Salmonella typhimurium in complex with the physiological ligand, (2R)-glyceryl-b-D-galactopyranoside pdf

Ngày tải lên : 23/03/2014, 04:21

... other residues in the authentic ribose-binding proteins In addition, the repressor sequences include a DNA-binding headpiece, and so are consistently longer than those of the binding proteins, even ... Domain of the two structures is superimposed (B) Superposition of domain in the available GBP structures from Salmonella and E coli The structures are colored progressing from blue (most closed) ... no structural sodium ion was noted at the same position in earlier GBP structures, from either Salmonella or E coli However, our inspection of the previous structures suggests that, in some cases,...

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Báo cáo khoa học: Analysis of the CK2-dependent phosphorylation of serine 13 in Cdc37 using a phospho-speciﬁc antibody and phospho-afﬁnity gel electrophoresis doc

Ngày tải lên : 30/03/2014, 03:20

... coprotein to an inactive catalytic subunit can activate a kinase In many signal-transducing protein kinases, site-specific phosphorylation by an upstream protein kinase (a kinasekinase) activates them ... MOK, v-Src and Raf1 were observed (Fig 9A), consistent with the result shown in Fig Interestingly, Cdc37 in the protein kinase complexes was detected as a single band in phospho-affinity gels (Fig ... kinase–Hsp90–Cdc37 complexes by phospho-affinity gel electrophoresis Finally, we analyzed complexes between signaling protein kinases and Hsp90–Cdc37 molecular chaperones by phospho-affinity gel...

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Báo cáo khóa học: Determination of modulation of ligand properties of synthetic complex-type biantennary N-glycans by introduction of bisecting GlcNAc in silico, in vitro and in vivo pot

Ngày tải lên : 30/03/2014, 13:20

... binding was measured, and the resulting Scatchard plots gave straight lines indicating the presence of a single class of binding sites in each case (not shown) To allow convenient comparisons, ... analysis of the data obtained used suitable software tools, as described previously [44,45] Lectin-binding assay Puriﬁcation of galactoside-speciﬁc lectins from dried leaves of mistletoe (Viscum ... processes at other sites in the chain through the shape alterations induced by its presence That bisecting GlcNAc constitutes a docking point for protein–carbohydrate interactions is known from...

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Báo cáo Y học: Expression of the uncoupling protein 1 from the aP2 gene promoter stimulates mitochondrial biogenesis in unilocular adipocytes in vivo potx

Ngày tải lên : 31/03/2014, 15:20

... US5476926) In conclusion, our results indicate that respiratory uncoupling per se is capable of inducing mitochondrial biogenesis in vivo They also support the hypothesis that respiratory uncoupling in ... differences in transgene expression that may be relevant for other studies, where the aP2 promoter is used to direct the expression of various genes into adipose tissue in mice (see also patent no US5476926) ... this tissue [32,40,41,44,45] These data suggest that respiratory uncoupling in adipocytes is associated with mitochondrial biogenesis However, possible existence of a causal link between these...

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báo cáo hóa học:" Function of anterior talofibular and calcaneofibular ligaments during in-vivo motion of the ankle joint complex" docx

Ngày tải lên : 20/06/2014, 01:20

... as shown in several reports.[4,18,19] This notion was supported by numerous in- vitro studies which investigated the changes in ATFL and CFL strain in response to loading in various positions ... demonstrated increased strain in the ATFL with increasing plantarflexion, inversion and internal rotation Strain in the CFL was found to increase in dorsiflexion and inversion Ozeki et al.[7] investigated ... processing, preparation of the manuscript and editing LW assisted with scanning, subject recruitment, image processing and data analysis HR supervised data analysis and interpretation, advised...

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Báo cáo hóa học: " Interaction Between Nano-Anatase TiO2 and Liver DNA from Mice In Vivo" pdf

Ngày tải lên : 22/06/2014, 00:20

... (HPMC, K4M) was used as a suspending agent Nano-anatase powder was dispersed onto the surface of 0.5%, w/v HPMC, and then the suspending solutions containing the TiO2 colloidal suspensions were treated ... theoretical functions by nonlinear least squares minimization of the residuals The data were analyzed using the EXAFSPAK analysis suite (http://wwwssrl.slac.stanford.edu/*george/exafspak/exafs.htm) together ... performed gel electrophoresis (Fig 6) Figure shows single strand DNA treated with various doses of nano-anatase TiO2, 123 Fig Assay of complete DNA from liver of mice in various nanoanatase TiO2 dose...

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Báo cáo khoa học: " The Antinociceptive and Anti-inflammatory Effect of Ethylacetate Extracts from Bang-Poong (Radix ledebouriellae) on the Freund’s Adjuvant-Induced Arthritis in Rats" pot

Ngày tải lên : 07/08/2014, 15:20

... mg/kg/day significantly suppressed arthritis-related symptoms BPE suppressed the adjuvant-induced paw edema during weeks in contralateral left hind paw and it also inhibited soft tissue swelling In ... group was strongly decreased (Fig 1, 2) Fos expression was also increased in both ipsi- and contra-lateral side of the spinal cord in RA-vehicle group Fos expression was usually applied as a neuronal ... his co-workers reported that the peripheral noxious stimulation increases the spinal Fos expression and it is reported that morphine, one of potent analgesics, reduces the spinal Fos expression...

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Báo cáo khoa học: "Two-dimensional gel electrophoresis of membrane proteins from ectomycorrhizal fungi" doc

Ngày tải lên : 08/08/2014, 23:22

... proteins by 2-D PAGE showed that the quality of silverstained gels obtained following the different methods of solubilisation differed widely (figs 3, and 5) Silver-stained gels of proteins solubilized ... 1984) Solubilisation buffers containing SDS/ acetone/urea-NP40 and SDS/NP40 resulted in good separation of microsomal polypeptides from C geophilum However, less background staining was observed ... omitted (Tasheva and Dessev, 1983) Proteins were silver-stained as Enzyme assays Membrane ATPase (ATP phosphohydrolase; EC 3.6.1.3) activity was defined as Mg-dependent ATP hydrolysis ATPase activity...

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