avoid float and double if exact answers are required
Báo cáo y học: "Two discrete events, human T-cell leukemia virus type I Tax oncoprotein expression and a separate stress stimulus, are required for induction of apoptosis in T-cells" pptx
... active forms To monitor the effects of Zn and Cd, we examined the integrity of pro-caspase 3, and in control and cation-treated Jurkat and JPX-9 cells When compared to control, there was little reduction ... with Cd and Jurkat cell treated with either Zn or Cd showed lower levels Y-axis is % apoptosis, and X-axis is hours after treatment B) and C) Cell viability was quantified using a modified MTT ... -8, and in Jurkat and JPX-9 cells were checked by Western blotting Jurkat and JPX-9 cells were treated with ZnCl2 or CdCl2 for 24 hours, and the indicated proteins were detected using specific...
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Tài liệu In this lab, 2 ISDN routers are required. If ISDN routers are not available, review the lab to become familiar with the process. An Adtran Atlas550 ISDN emulator is used to simulate the switch/ISDN cloud. pdf
... Tokyo(config -if) #interface bri Tokyo(config -if) #encapsulation ppp Tokyo(config -if) #ppp authentication chap Tokyo(config -if) #interface dialer Tokyo(config -if) #encapsulation ppp Tokyo(config -if) #ppp ... a Dialer Pool, and put the interfaces and the associated Dialer Profiles in a common pool The commands for doing this are as follows: Tokyo(config -if) #interface bri Tokyo(config -if) #dialer pool-member ... Tokyo(config -if) #interface dialer Tokyo(config -if) #dialer pool Tokyo(config -if) #interface dialer Tokyo(config -if) #dialer pool b On Moscow, the commands issued would be as follows: Moscow(config -if) #interface...
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Tài liệu Báo cáo khóa học: Further insights into the assembly of the yeast cytochrome bc1 complex based on analysis of single and double deletion mutants lacking supernumerary subunits and cytochrome b pdf
... arrows labeled (b) and (c) point to the areas of interaction between the transmembrane helix of subunit and helices G and H1 of cytochrome b and between the N-terminus of subunit and helix a of cytochrome ... The double deletion strains were constructed as follows The haploid strains VZ1 (D7) and MES8 (D6), VZ2 (D7) and LLD9 (D8), JDP1 (D9) and LLD9 (D8), JDP2 (D9) and UBL2 (D10), were mated and the ... have prepared single and double deletion yeast mutants in which one or two nuclear genes encoding the supernumerary subunits Qcr6p, Qcr7p, Qcr8p, Qcr9p and Qcr10p have been deleted and analyzed...
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Tài liệu Báo cáo khoa học: Characterization of promoter 3 of the human thromboxane A2 receptor gene A functional AP-1 and octamer motif are required for basal promoter activity docx
... tion and cells lysed and prepared for luciferase assays as described above HEK293 and HEL cell supernatants were assayed for both firefly and renilla luciferase activity using the Dual Luciferase ... TPa and TPb are differentially expressed and are under the transcriptional control of two distinct promoters, Prm1 and Prm3, respectively [25], greatly adds to the complexity of TXA2 signaling and ... Prm3aaAP)1* and Prm3aaaAP)1*, where the AP-1 element centered at )27 was mutated, were cotransfected with pRL-TK into HEL92.1.7 (A and B) and HEK293 (C and D) cells Firefly and renilla luciferase...
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Tài liệu Báo cáo khoa học: The diacylglycerol and protein kinase C pathways are not involved in insulin signalling in primary rat hepatocytes doc
... anti-a, -b, -c, -d, -e, and -f for immunoblotting were obtained from Gibco (Grand Island, NY, USA) Rabbit anti-PKCf for immunoprecipitation and activity determination, and the antiPKCf blocking ... fluoride, 20 mM 2-mercaptoethanol) and centrifuged at 100 000 g for 30 Supernatant and membrane fraction were diluted with lysis buffer (without sucrose and EGTA) and 8–14 lg of protein from each ... rapidly aspirating the medium and immersing the dishes in liquid N2 Glycolysis and glycogen synthesis Materials and methods Materials Enzymes, M199 medium, collagenase A and the transfection agent...
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Tài liệu PC Security Handbook: Defensive Computing Techniques to Help You Avoid Malware and Data Loss ppt
... but it’s hard to know exactly what they mean Adware – Like spyware, adware is software that may track visited websites and act as a key logger Adware tracks this PC Security Handbook 2nd Edition ... malicious software Privacy-invasive software – A formal term used to describe software that invades your privacy This software comes in different forms including spyware and adware Real-time Scanning ... wonder why you are being offered “PC Super Anti Spyware 2011” when using your PC; this is adware at work AIM, FlashGet, Deamon Tools, and RealPlayer are all examples of adware Antimalware / Antivirus...
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Báo cáo khoa học: Amino acids at the N- and C-termini of human glutamate carboxypeptidase II are required for enzymatic activity and proper folding pptx
... both in cell lysates and conditioned media, and the results are summarized in Fig and Table 2, respectively Of the 13 variants, only 274/587 (the putative catalytic domain) and 274/750 (the polypeptide ... Three days later, the cells and conditioned media were harvested and processed as described in the Materials and methods Conditioned media were dialyzed and concentrated, if desired Carboxypeptidase ... Antibodies Hybridomas secreting mAbs (clones GCP-01, GCP-02 and GCP-04, all IgG1) were prepared by standard methods from mice (F1 hybrids of BALB/c and B10.A strains) immunized with recombinant human...
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Báo cáo khoa học: Saccharomyces cerevisiae Ybr004c and its human homologue are required for addition of the second mannose during glycosylphosphatidylinositol precursor assembly ppt
... intermediates by separate candidate GPI mannosyltransferases (GPIMT) The mammalian Pig-M protein and its yeast ortholog Yjr013wp are required for addition of Man1 to GPI precursors [19], and the PIG-B ⁄ ... grown and [3H]inositol-labeled in glucose-containing medium Lipids were extracted from cells and incubated either with or without mild-base (lanes and 2) and with or without PI-PLC (lanes and 4) ... criteria First, because GPI-MT I, III, and IV are integral membrane proteins having at least eight transmembrane domains and overall lengths between 403 and 678 amino acids [25], we eliminated...
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Báo cáo khoa học: Pyruvate:ferredoxin oxidoreductase and bifunctional aldehyde–alcohol dehydrogenase are essential for energy metabolism under oxidative stress in Entamoeba histolytica pdf
... centrifuged and resuspended in normoxic (0.18 0.09 mM O2 at 36 C and 2240 m altitude) NaCl Pi + glucose and returned to the water bath At different time intervals, samples were centrifuged, and ... At different times, the samples were treated for 20 with 10 units of Cat and EhPFOR was reactivated for 30 at C with mM Fe2+ under reducing and anaerobic conditions For (A) and (C), data are ... exposed and unexposed to the O2 and during recovery under normoxic conditions Data analysis Data are reported as means standard deviations (SDs), except in Fig 4B,C, where means standard errors...
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Báo cáo khoa học: The sulfur atoms of the substrate CoA and the catalytic cysteine are required for a productive mode of substrate binding in bacterial biosynthetic thiolase, a thioester-dependent enzyme doc
... terminal sulfur are coloured green, hydrogen bonds are coloured red, and interactions between hydrophobic side chains and the planar amide bonds of CoA are coloured yellow b-strand Cb2, and Met134 ... unliganded thiolase; in the case of a bound CoA ligand, it points towards the ligand This difference correlates with minor conformational differences between the pantetheine moieties of CoA and ... thiolase at 25 °C The values and error estimates are calculated from separate measurements (three for the wild-type and two for C89A) Ka and Kd are the association and dissociation constants,...
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HOW TO AVOID SCAMS AND FRAUD pptx
... misleading and deceptive offers and to help you protect yourself from different scams and forms of fraud The information and tips in this booklet can help you avoid falling victim to fraud and protect ... the current residents and their families to see if they are happy with the care they are receiving Ask to review a copy of the policies and procedures regarding the rights and responsibilities ... membership? What specific discounts are available to the consumer from participating providers, under what circumstances, and are they guaranteed? Are there any additional fees which are charged as...
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Báo cáo khóa học: Large aggregating and small leucine-rich proteoglycans are degraded by different pathways and at different rates in tendon pot
... intact core protein of aggrecan and V0 and/ or V1 splice-variants of versican [1] With time in culture, a distinct band at 300 kDa (indicated by asterisk) appeared, and remained in the matrix over ... associated with the band ranging between 37 and 45 kDa This band corresponds to the decorin core protein; also present are small levels of biglycan core protein [1] A number of bands were also observed ... distinct bands at 25 kDa and below (lanes ii and iii) These observations indicate that degradation of core proteins of newly synthesized small proteoglycans occurs and that fragments are retained...
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INDUSTRY SURVEY - What future animators say about what they are looking for in a school, and what professional animators say are the most important things to look for. ppt
... who are working studio animators, stand in for directors, and assignments stand in for film or game shots Students learn how to accept guidance and criticism, to meet deadlines, and to budget and ... industry, animation careers, and advice and tips from our mentors In addition, you can see what Animation Mentor students are working on and get the inside scoop on how they are creating animation ... TIPS AND TRICKS, VOLUME II More tips and tricks from Animation Mentor founders and mentors including Shawn Kelly, Carlos Baena, Keith Sintay, Aaron Gilman and Wayne Gilbert WWW.ANIMATIONTIPSANDTRICKS.COM...
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book of exercises to accompany e. friedrich richter's manual of simple and double counterpoint (1888)
... "Manual of Simple and Dotible Counterpoint" it is (p 16, No 4) that ^^Modulations are to be avoided, except in rare cases, where, for special reasons, those leading into the nearest related keys ... teacher soon learns to know which subject offers most pupils special difficulties and requires a more diffuse explanation, and which is that subject that, as being easily understood by the average ... perfectly clear and self-evident to him must be so to every one else also From this it may come that now and then little talent achieve better results than those of great gifts and eminent knowledge...
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Báo cáo sinh học: " The herpes simplex virus UL20 protein functions in glycoprotein K (gK) intracellular transport and virus-induced cell fusion are independent of UL20 functions in cytoplasmic virion envelopment" docx
... Intracellular transport and TGN localization of UL20p mutants and gK Transport and localization of UL20p and gK was further assessed by transient coexpression of gK and UL20p and simultaneous detection ... production and virus-induced cell fusion The results suggest that virus-induced cell fusion mechanisms are not required for cytoplasmic virion envelopment Materials and methods Cells and viruses ... Similarly, the Y38A – Y49A double mutant combined the two specific tyrosine modifications without altering adjacent amino acids In addition, UL20 mutants CL2, CL61, Y38A, and Y117A, which were not...
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Báo cáo sinh học: " Transcription and translation of human F11R gene are required for an initial step of atherogenesis induced by inflammatory cytokines" doc
... http://www.translational-medicine.com/content/9/1/98 Page of 14 a * HAEC HAEC TNFα&IFNγ α γ * IFNγ γ TNFα α Untreated IFNγ γ * HUVEC TNFα&IFNγ α γ IFNγ γ * * TNFα α Untreated TNFα&IFNγ α γ IFNγ γ TNFα α * * TNFα&IFNγ α γ * * * TNFα α * b Untreated ... treated for 0, 3, 6, 12, and 24 hrs with TNFa (100 u/mL) and/ or IFNg (200 u/mL), and in cultured HUVEC (bottom panels) treated for 0,4,8,12, and 24 hrs with TNFa (100 u/mL) and/ or IFNg (200 u/mL) Real-time ... untreated venous and arterial cells (prepared as cell lysates) as a protein of 37 kDa, and following the treatment of venous and arterial endothelial cells with TNFa and/ or IFNg, F11R continued...
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báo cáo hóa học: " Vascular endothelial growth factor-A and chemokine ligand (CCL2) genes are upregulated in peripheral blood mononuclear cells in Indian amyotrophic lateral sclerosis patients" pot
... univariate and multivariate logistic regression respectively to check any possible influence of smoking, alcohol and meat consumption on VEGF-A and CCL2 mRNA levels and c (chi square) test was ... compared to patients without respiratory dysfunction (Figure 3A-B; p = 0.045 and p = 0.021 respectively) No association of cigarette smoking, alcohol and meat consumption with VEGF-A (Table 2) and ... in ALS and in its different clinical subtype with limb onset and extended disease duration are in agreement with current results [18,19] However, some studies have failed to detect significant...
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