... used the construct pGEMT–5¢HumanCPT1B as a template in a PCR reaction with primers DH673 (5¢-AGCTGAATTC ATGGCGGAAGCTCACCAG-3¢) and DH803 (5¢-TCCA CCCATGGTAGCAGAGAAGCAGCTTAAGGGTTTGG CGGA-3¢) The ... The assay was performed at mm carnitine as standard To analyze PigE17DCPT1B and HumanD17ECPT1B mutants, the assay was performed at carnitine concentrations equal to the Km The percentage of activity ... concentrations of carnitine: mm (for standard comparison with other published data), and a concentration equal to the Km for carnitine of each enzyme (for comparison between mutants) In this article,...
... proposed that the WD protein acts as a copperspecific pump that mediates the export of copper from the cytosol, similar to the P-type ATPase [14] On the basis ofthe fact that MREs mediate the transcriptional ... Taq (Takara, Japan) in a total volume of 50 lL containing 55 pg of competitor At this concentration, the band intensity ofthe amplified product from the competitor was same as that ofthe product ... normalized to b-gal activity, and the relative increases were calculated as the ratio of normalized activity in MREa/mutant transfected cells to that in pGLpro transfected cells Data represent the...
... n .a n .a n .a Age is presented as mean and range of values (n .a. : not applicable) Page of (page number not for citation purposes) Journal of Translational Medicine 2009, 7:100 http://www.translational-medicine.com/content/7/1/100 ... 0.052 Data are presented as mean and range of values death due to severe sepsis compared to patients without allele CATT7 The concomitance ofthe -173 allele C and the -794 allele CATT7 as a haplotype ... After approval ofthe local ethics committee and written informed consent ofthe patient or a legal guardian had been obtained, 169 Caucasian patients with the diagnosis of severe sepsis according...
... preprocessing and then algorithm of Klein [9] (for planar distances) • Let F be the set of edges of G intersected by the (dual) edges of C ∗ Then G − F is actually a plane embedding, and we easily add the ... γ then define in this order the vertex set ofa noncontractible dual cycle C ∗ , and so ≤ |V (C ∗ )| ≤ r∆/2 Proof of Theorem 1.2 The idea ofthe previous statement readily translates into an approximation ... constant factor ∆2 /(8cg ) of crg (G) Remark 4.2 In the planar case of Theorem 1.2, the described approximation algorithm yields a drawing of G within a factor 4.5∆2 of cr0 (G) References [1] D Bokal,...
... examination revealed abatement ofthe disease Indeed, the last laparotomy performed did not reveal any disease Second, the pathology report indicated that the patient had an epithelioid type of ... laparotomy during which division of adhesions and repair ofa hernia was carried out No evidence of mesothelioma was found at this stage, and two months later, laparotomy was repeated for division ... differential diagnosis, the extent ofthe disease and the presence of invasion mitigated against this diagnosis The immunohistochemical (ICH) findings are shown in Table Two years later, the patient...
... examination revealed abatement ofthe disease Indeed, the last laparotomy performed did not reveal any disease Second, the pathology report indicated that the patient had an epithelioid type of ... laparotomy during which division of adhesions and repair ofa hernia was carried out No evidence of mesothelioma was found at this stage, and two months later, laparotomy was repeated for division ... differential diagnosis, the extent ofthe disease and the presence of invasion mitigated against this diagnosis The immunohistochemical (ICH) findings are shown in Table Two years later, the patient...
... β-actin (forward): AGCAAGCAGGAGTATGACGAGTC, β-actin: AGAAAGGGTGTAACGCAACTAAGTC (reverse), CSF1R(forward): TTCTGCTGCTCCTGCTGGTG, CSF1R(reverse): ACCGTTGCTCCTGGCTTCAC, LOX1(forward): ACTGTGAAGGACCAGCCTGATG, ... responsible forthe pattern observed in the dendrogram, a two-way ANOVA was fit to each probeset using activation and knockdown state as explanatory variables A linear contrast analysis was then performed ... YW and XQ constructed and characterized the HIV-1 viruses and lentiviral vectors used in the study CS performed the analysis of DNA microarray data and performed the statistical analysis AR conceived...
... found that Tax1 and Tax2 transform a rat fibroblast cell line (Rat-1) to induce colonies in soft agar (CFSA, colony formation in soft agar), and the activity of Tax1 is greater than that of Tax2 ... We also thank Sayoko Takizawa and Chika Yamamoto forthe excellent technical assistance This work was supported in part by a Grant-in-Aid for Scientific Research on Priority Areas and a Grant-in-Aid ... the manuscript Acknowledgements We thank William W Hall for donating the Tax2B plasmid and the antibody against Tax2B We thank the Takeda pharmaceutical company for providing recombinant human...
... correct Standard deviation isthe property ofthe normal curve Mathematically, it isthe square root ofthe variance ofa test Test analysis isthe data from test trials are analyzed during the test ... selection of participants, the materials and the methods of data collection and analysis as well the results ofthe process of data analysis Part three: Conclusion – this part will be the summary to the ... has sometimes been regarded in the past as something additional and in no way an integral part ofthe syllabus or examination (p 15) 1.1.1.2 The structuralist approach “This approach is characterized...
... as described in Materials and methods Enzyme activity was analysed by the standard assay and the immunoreactivity was detected by Western blot analysis and quantitated as described in Materials ... respectively The fragment originating from the rat gene is indicated by a hatched bar The activity in cell extracts of stable transformants was measured as described in Materials and methods The values ... indicated by asterisks to activate the material, as previously described [15] However, all attempts so far to associate this material have failed Thus, it appears that the isolated Arg252-containing...
... the Earth’s atmosphere as measured at the top of Mount Mauna Loa on the Island of Hawaii Human activities are responsible for this dramatic rise This increase in greenhouse gases is causing an ... unsafe water isa major problem at all times To give an indication ofthe scale of health problems caused by lack of safe water, there are approximately billion cases of diarrhoea each year, causing ... 1994 for 18 Pacific Island countries There was a positive association between annual average temperature and the rate of diarrhea reports, and a negative association between water availability and...
... means that the measurement values are related to a certain cell This is meaningful because several cells share the same Iub interface and in the case of softer handover they also share the same ... it is just a counter value, however, the gross margin isa KPI Hence, what makes the difference between performancerelated data and a KPI isthe fact that a KPI is computed using a formula There ... analysis of huge data streams supplied by signalling information and user data/payload As a result the idea ofa second book was already born before the first one was ready to be published Some major...
... end of an a- helical region ofthe HFE a1 domain (V100 and W10 3A) as being of particular importance for TfR1 interactions [16,17,20] The effect of HFE binding to TfR1 is to lower the affinity ofthe ... nevertheless argue against the existence ofa single glycosylation site that isof such absolute importance that it is capable in its own right of regulating localization, b2M interactions or transferrin ... N-Glycosylation of HFE TGGGCGTGATTTTCCAT To generate the pEP7–HFEN13 0A HA mutation we used the following primer set: sense, ATGCAAGAAGACGCCAGTACCGAGGGC; antisense, GCCCTCGGTACTGGCGTCTTCTTGCAT To generate...
... (5¢-cggagaugacgg-3¢), 29 base DNA13-RNA4(5¢-AATAGAGAAAAAGaaaaAAGATGGCAA DNA12 AG-3¢), 29 base DNA15-RNA1-DNA13 (5¢-AATAGAGAA AAAGAAaAAAGATGGCAAAG-3¢) and 3¢-FAM-labeled 18 base RNA9-DNA9 (5¢-uugcaugccTGCAGGTCG-3¢) ... TGGGTTTGAGAGCATATGAAGTTGG CAAAAAAATACTAC-3¢ for primer 1, 5¢- CGCATATG GAGACGATGATCGCCTACGTCGATG-3¢ for primer 2, 5¢-ACCGTTAAGCTTTCATAAACATCCTCCTTT-3¢ for primer 3, and 5¢- CGGAATTCTCATGTGTCCAGTTCTG GACAGATGCACTC-3¢ ... activity The binding affinity of Tma-W2 2A to the R29 ⁄ D29 substrate was analyzed in the absence ofthe metal cofactor using surface plasmon resonance and compared with that of Tma-RNase HI The KD value...
... were 5¢-AGCAGAATAA CTGCTCTCCTGGTG-3¢ (forward) and 5¢-CACCAGGAG AGCAGTTATTCTGCT-3¢ (reverse), and those for MscL F54C were 5¢-GGGATCGATTGCAAACAGTTTGC-3¢ (forward) and 5¢-GCAAACTGTTTGCAATCGATCCC-3¢ ... via the YidC insertase [6] Hence, it is likely that specific characteristics ofthe transmembrane pairs are also critical forthe conditions of membrane insertion Analysis ofthe biogenesis of ... extraction ofthe IMVs to distinguish integral and peripheral membrane proteins T, total IMV sample; S, carbonate supernatant fraction; P, carbonate pellet fraction (B) As a control forthe carbonate...
... calculate the optimal value of k, and the necessary length ofthe hashed array, to achieve any desired bound on the error rate In general, the hashed array can be much smaller than the original ... a constraint satisfaction problem like this one, we can ask two questions: is there a feasible solution, assigning values to the variables so all constraints are satisfied; and if so what isthe ... successor of exactly one other element ULs are special because their set programming constraints always take a particular form: if x isa UL and a successor of y, then the constraints on its set Sx are...
... the head of each phrase and the grammatical functions: TIME, SUBJ(ect) and P-0BJ(ect) Evaluation The performance ofthe parser partly depends on the output ofthe tagger and the rest ofthe preprocessing ... preprocessing software Our way of dealing with how "correct" the performance ofthe parser is, follows a practical, pragmatic approach, based on consultation of modern Swedish syntax literature We use the ... Clauses, Grammar3 a n d Grammar4: the clause resolution is based on surface criteria, outlined at the beginning of this chapter, and the rather fixed word order of Swedish Grammar3 distinguishes different...
... thousands of deaths Salmonellosis is caused by the bacteria Salmonella Today, there are over 2500 known types, or serotypes, of Salmonella.Salmonellae are found in the intestinal tracts of animals ... Children and Food Safety PROTOZOA Toxoplasma gondii Giardia lamblia Entamoeba histolytica 16 Some protozoa that cause foodborne diseases are Toxoplasma gondii, Giardia lamblia, and Entamoeba histolytica ... soil; they can persist in soil for 10 years or more The eggs have a lipid layer, containing ascarocides and it makes them resistant to the effects of acids and alkalis as well as other unpleasant...
... principal investigators 12 in pancreatic cancer, who are each an as- 13 sistant-level professor in a major academic 14 research institution and who have each re- 15 ceived at least grant from the National ... rector ofthe National Cancer Institute in ac- cordance with paragraph (5) regarding the prioritization and award of National Institutes of Health research grants relating to pancreatic 10 cancer ... 30 days after the establishment ofthe Committee, the Secretary shall publish the names ofthe Chair and members ofthe Com- mittee on the Website ofthe Department of Health and Human Services...
... by the addition of mm EDTA and SDS ⁄ PAGE loading buffer, followed by heating in a boiling water-bath forThe amount of r32 that remained after proteolysis was analyzed after SDS ⁄ PAGE ofthe ... Control of phage lambda development by stability and synthesis of cII protein: role ofthe viral cIII and host h A, himA and himD genes Cell 31, 565–573 Rattray A, Altuvia S, Mahajna G, Oppenheim AB ... partial inhibition was achieved As in the case of CII [10,11], CIII appears to work as an inhibitor forthe proteolysis of r32 through direct interaction with the protease, characterized by a...