assessment of hepatotoxicity potential of drug candidate molecules including kinase inhibitors by hepatocyte imaging assay technology and bile flux imaging assay technology
Tài liệu Comparison of lung cancer cell lines representing four histopathological subtypes with gene expression profiling using quantitative real-time PCR pptx
... Quantification of 19 candidate genes by qPCR Using qPCR, we quantified the expression of the 19 candidate genes in the same eight test cell lines (A549, ABC-1, EBC-1, LK-2, LU65, LU99, STC 1, RERF-LCMA) and ... not extracted by IPA The connection including CDH1, PLAU, and SMAD4 suggested to be related to cell adhesion by IPA The connection including TGM2, IL1B, and PLAU and the connection including RAB25, ... expression of STMN1, AMY2A, and ISL1, and positively correlated with S100P, RAB25, and CDH1 The loading number of component (PC3) was negatively correlated with the expression of RAB25 and AMY2A, and...
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Tài liệu Báo cáo khoa học: A differential scanning calorimetry study of tetracycline repressor pptx
... concentration of 0.6 mgặmL)1 and scan rate (v) of 0.12.0 Kặmin)1 Measurements of liganded protein were performed in buffer B, at a protein concentration of 0.4 mgặmL)1 and at vefold molar excess of Tc ... Tmax, of the complex of TetR with Tc is 70.4 C, measured at a protein concentration of 0.4 mgặmL)1 and scan rate of Kặmin)1 (Figs and 2) Therefore, under these experimental conditions, binding of ... determined by the total enthalpy of the transition, which is assumed to be a constant multiplied by the fraction of the molecules that are unfolded: H(T) ẳ fu(T)DH The fraction of unfolded molecules...
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Applications of Calorimetry in a Wide Context - Differential Scanning Calorimetry, Isothermal Titration Calorimetry and Microcalorimetry docx
... editor of the book for my experience and publications in the field of applications of calorimetry and biocalorimetry in the analysis of small and large drug molecules I welcomed the invitation and ... Department of Pharmacy, Health and Well-being, University of Sunderland, UK; Dr Eman Ali Elkordy, Faculty of Medicine, University of Tanta, Egypt; Prof Gamal El Maghraby, Faculty of Pharmacy, ... slopes of 0.65 and –0.20 for short and extended milling times, respectively, in the case of Fe; and slopes of –0.85 and –0.03, respectively, for short and extended milling times in the case of Fe50Co50...
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Báo cáo khoa học: Purification and structural study of the b form of human cAMP-dependent protein kinase inhibitor pdf
... concentration of the proteins was determined by the method of Lowry et al [14] and the purity was examined by SDS/PAGE [15] Assay of human PKIb activity The activity of purified human PKIb was assayed by ... indicated by the presence of a very strong negative band at 198 nm The negative band near 220 nm results from overlapping of the bands of b-sheet (215 nm) and a-helix Table Fractional composition of ... Q & Zhao, S (2000) Cloning and mapping of human PKIB and PKIG, and comparison of tissue expression patterns of three members of the protein kinase inhibitor family, including PKIA Biochem J 349,...
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Báo cáo Y học: Differential scanning calorimetric study of myosin subfragment 1 with tryptic cleavage at the N-terminal region of the heavy chain pdf
... by the addition of mM MgCl2 F-Actin was stabilized by the addition of a twofold molar excess of phalloidin (Sigma) to obtain a better separation of the thermal transitions of actin-bound S1 and ... Preparation of the complexes of t-S1 and Nt-S1 with ADP and Pi analogs Trapping of ADP by different phosphate analogs (Vi, BeFx) was performed by the methods described for the preparation of stable ... obtained by addition of 0.5 mM BeCl2 in the presence of mM NaF The formation of the complexes was controlled by measuring the K+-EDTA ATPase activity of the protein The ATPase activity of S1, t-S1,...
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selfridge, arnold, warnick. teaching electromagnetic field theory using differential forms
... Deschamps 2], and Burke 3] These works note some of the advantages of the use of di erential forms in EM theory Misner et al and Burke treat the graphical representation of forms and operations ... surfaces of a 1-form are oriented as well This is done by specifying one of the two normal directions to the surfaces of the form The surfaces of dx are oriented in the +x direction, and those of ?3 ... 2-Forms; Flux Density and Current Density Flux density or ow of current can be thought of as tubes that connect sources of ux or current This is the natural graphical representation of a 2-form,...
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Differential scanning calorimetrycalorimetry
... types of DSC instruments: heat -flux and power compensation 1Haines, P J (2002) The Royal Society of Chemistry, Cambridge EYP 2006 Heat Flux DSC Sample holder : sample and reference are connected by ... Disadvantages: uncertainty of heats of fusion and transition temperatures “Hyphenated” Techniques • thermal techniques alone are insufficient to prove the existence of polymorphs and solvates • other ... computer to control data collection and processing EYP 2006 TGA and DSC Thermogravimetric Analysis (TGA) – mass change of a substance measured as function of temperature whilst the substance...
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báo cáo hóa học:" Preclinical evaluation of dasatinib, a potent Src kinase inhibitor, in melanoma cell lines" pptx
... the design of the study and carried out the proliferation assays, TUNEL assays, cell cycle assays, Western blotting and statistical analysis JC and MC contributed to the interpretation of the data ... Effect of dasatinib on Src kinase, EphA2 and FAK Src, EphA2, FAK and phosphorylated Src, EphA2 and FAK were detected in all cell lines tested, although the levels of phosphorylated Src kinase ... cell lines Effect of4 Effect of dasatinib on (A) invasion and (B) migration in HT144 and Sk-Mel-28 melanoma cell lines Error bars represent the standard deviation of triplicate assays '*' indicates...
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Báo cáo hóa học: " Temperature-Dependent Site Control of InAs/GaAs (001) Quantum Dots Using a Scanning Tunneling " pptx
... of a dot grown in a hole structure under In and As4 irradiations at 300°C and its line profiles a Shows the hole structure, b and c were images after supplying additional 0.02 ML and 0.04 ML of ... structure was smaller than that of Fig 1(b’) This result indicated that the size of the surface structure transition can be controlled by the amount of voltage, and it needs at least 150°C Surface ... about 170 nm of a GaAs buffer layer was grown at 560°C under Ga and As4 fluxes, 1.0 10-5 Pa and 6.0 10-4 Pa, respectively After the GaAs buffer layer growth, about 1.5 monolayers (ML) of an InAs...
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Báo cáo vật lý: "EXOTHERMIC TRANSITIONS ON COOLING OF GELATINIZED NATIVE RICE STARCH STUDIED BY DIFFERENTIAL SCANNING CALORIMETRY" doc
... transitions were broad and flatten with Tcxo of ~119oC for 1:1, sharpen prominently and bigger for 1:2 but became slightly smaller and broader for 1:3, with Tcxo of ~89oC and 85oC, respectively ... enthalpy of exotherm of 1:2 system was higher compared with the enthalpy of exotherm of 1:3 system, was most probably due to concentration effect, in which the availability of amylose and lipid ... cooling curves of native and defatted rice starches are presented in Figures and Defatting did not appear to alter the main gelatinization temperature of rice Thermal Transitions on Cooling of Rice...
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Báo cáo y học: " Local treatment with the selective IκB kinase β inhibitor NEMO-binding domain peptide ameliorates synovial inflammation" pot
... absence of NBD peptides (100 µM) After days, supernatants were collected and IL-6 levels were measured by sandwich ELISA and corrected for weight of the biopsy Data are representative of three ... studies and cell culture and performed immunohistochemical stainings and digital image analysis MJM and SG participated in the design of the study and helped to draft the manuscript PPT conceived of ... absence of NBD peptides (100 µM) and after 2hour pre-incubation stimulated with rhTNF-α (10 ng/ml; R&D Systems) After days, supernatants were collected and evaluated for the presence of IL-6 by sandwich...
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Báo cáo y học: "Metalloproteinase and inhibitor expression profiling of resorbing cartilage reveals pro-collagenase activation as a critical step for collagenolysis" pot
... assayed by the addition of samples to a known amount of active collagenase in the diffuse fibril assay One unit of collagenase activity degrades μg of collagen per minute at 37°C, and one unit of ... synthesis and activation of pro-collagenases, causing almost complete resorption of human and bovine nasal cartilage in a short assay period [19,20] Natural and synthetic metalloproteinase inhibitors ... of metalloproteinases and their inhibitors and correlate this with pro-collagenase activation and aggrecan and collagen release Materials and methods Cartilage degradation assay Bovine nasal cartilage...
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Báo cáo khoa học: " The c-Met receptor tyrosine kinase inhibitor MP470 radiosensitizes glioblastoma cells" ppt
... treatment for the treatment of GBM A recent review of this approach indicates that several novel inhibitors of the tyrosine kinase activity of cMet have been developed and tested as a single agent ... Figure MP470 and radiation on clonogenic survival of eight GBM cell lines Effect of1 Effect of MP470 and radiation on clonogenic survival of eight GBM cell lines The combination of MP470 (5 μM) ... tyrosine kinase encoded by the proto-oncogene c-Met In malignant cells, HGF has been shown to protect cells from death induced by a variety of DNA-damaging agents, including radiation and topoisomerase...
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Báo cáo y học: "Reliability of capturing foot parameters using digital scanning and the neutral suspension casting technique" ppt
... plantar surface of the foot of 400 mm Six plaster casts and six digital images were taken of the left foot of all participants Three casts of the left foot were taken by rater followed by rater with ... was granted by the Auckland University of Technology Ethics Committee (AUTEC) Informed consent was given by all participants Examiners Casting and digital scanning was performed by an undergraduate ... manufacture of, and successful outcome of, orthotic intervention The clinician should also be aware of the potentially small measurement errors when utilising digital scanning Therefore, the aims of this...
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báo cáo khoa học: "ABT-869, a promising multi-targeted tyrosine kinase inhibitor: from bench to bedside" doc
... range of kinases Kinase inhibition profile of ABT-869 against a broader range of kinases Nonclinical in vivo activity of ABT-869 Initial nonclinical studies demonstrated potent antiproliferative and ... against most of the targeted kinases but these inhibitors demonstrate more off-target activity than ABT-869 [18] Another potentially important aspect of the distinctive activity profile of ABT-869 ... 0.004) by day 15 of treatment (0.25 mg/kg) [64] The biomarker evidence of antiangiogenic activity and DCE-MRI evidence of tumor antiangogenesis are consistent with proof of target inhibition and...
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Báo cáo y học: "Inhibitory effects of ZSTK474, a novel phosphoinositide 3-kinase inhibitor, on osteoclasts and collagen-induced arthritis in mice" pot
... survival and motility of OCs has been demonstrated by using the PI3K inhibitors wortmannin and LY294002 [15-22], and also by studying mice deficient in the expression of the p85α subunit of class ... of IκB and phosophorylation of JNK and ERK1/2 induced by sRANKL On the other hand, the expression of NFATc1, which occurs in the late phase of OC differentiation and promotes terminal osteoclastogenesis ... inhibition of OC differentiation Inhibitory effects of ZSTK474 on OC formation induced by both RANKL and TNF-α We next examined the effects of ZSTK474 on OC formation induced by RANKL and TNF-α,...
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Báo cáo y học: " Inhalation of the Rho-kinase inhibitor Y-27632 reverses allergen-induced airway hyperresponsiveness after the early and late asthmatic reaction" pps
... Activated Rho -kinase interferes with the equilibrium of MLCK and MLCP activities by phosphorylating and thereby inactivating the myosin binding subunit of MLCP This leads to an augmentation of MLC20 ... histamine and PGF2α before and after the allergen-induced EAR and LAR We demonstrate that there is a differential role of Rho -kinase in the agonistinduced airway obstructions and that inhalation of ... contraction Thus, the potency and maximal effect of histamine-induced contraction of guinea pig tracheal smooth muscle preparations were unaffected by inhibition of Rho -kinase, whereas these parameters...
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Báo cáo sinh học: "Characteristics of predictor sets found using differential prioritization" docx
... case of our OVA toy dataset; any subset of suf- Next, initialize a matrix M: = (μi, k)N × K of zeros where N is the total number of genes in the dataset, and, in this case, is the product of G and ... given pair of classes, a group of marker genes only distinguishes samples from one class of the pair of classes against samples from the other class of the pair of classes As implied by its name, ... values of the DDP which optimize dE,α , dMP,α , and (17) VS represents the relevance of S, while US represents the antiredundancy of S VS measures the average of the correlation of the members of...
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Báo cáo y học: "The Bruton tyrosine kinase inhibitor PCI-32765 ameliorates autoimmune arthritis by inhibition of multiple effector cells" ppsx
... http://arthritis-research.com/content/13/4/R115 kinase) , JNK (c-Jun N-terminus kinase) , and MAPK (mitogen-activated protein kinase) Consistent with these observations, phosphorylation of Btk and PLCg and calcium mobilization of FcgR ... inflammation, bone erosion, and autoimmunity in vivo by affecting the function of multiple immune cells involved in both the propagation and effector phases of CIA Materials and methods Drug formulation ... formation of ICs: a final concentration of 10 μg/mL of Fb and 50 μg/mL of antibody were used for IC stimulation of RAW 267.4 cells At final dilutions, all reagents used in the stimulation assays...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các mục tiêu của chương trình khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ rôto dây quấn đặc tuyến hiệu suất h fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha phần 3 giới thiệu nguyên liệu chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25