assembly cell polarization and cell survival pdf
báo cáo hóa học:" CCR9 interactions support ovarian cancer cell survival and resistance to cisplatin-induced apoptosis in a PI3K-dependent and FAK-independent fashion" pdf
... direct leukocyte and cancer cell migration, and play pivotal roles in cell survival [9] Studies have demonstrated that CXCR4-CXCL12 interactions promote the survival of tumor cells, allowing growth ... for 24 hours The cells were harvested and washed in cold PBS and the cell density was adjusted to 106 cells/ml Subsequently, cells were stained with Annexin V and Propidium Iodide (PI) using the ... cell survival Methods Cell Lines and cell culture Human OvCa cell line, OVCAR-3, was obtained from the ATCC The cells were cultured in RPMI 1640 (Mediatech, Inc.) at 37°C and 5% CO2 with 10% fetal...
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Báo cáo khoa học: Two functionally redundant isoforms of Drosophila melanogaster eukaryotic initiation factor 4B are involved in cap-dependent translation, cell survival, and proliferation doc
... S2 cells during starvation showed that eIF4B is required for cell survival, additional eIF4B reduction by RNAi treatment may explain why the cells died In yeast, eIF4A is required for cell survival ... medium was removed, and the cells were resuspended in 4.5 mL medium, counted, and split into four dishes At 24 h after transfection, the medium was removed from two wells, and the cells were subjected ... of Dm-eIF4B and Sch pombe Sce3p are 44% and 36%, respectively, and 34% and 24% between amino acids 157–419 of Dm-eIF4B-L and both eIF4B1 from A thaliana and eIF4B from wheat, respectively (not...
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Báo cáo khoa học: Molecular and functional characterization of a novel splice variant of ANKHD1 that lacks the KH domain and its role in cell survival and apoptosis docx
... tested cell lines and this siRNA was used in subsequent assays Following transfection of VBARP siRNA into HeLa and NT2 cells, physical observation revealed that cell death occurred in a dose- and cell- dependent ... HEK293 T cells expressing Vpr and VBARP by cotransfecting Vpr and VBARP-His using calcium phosphate transfection Forty-eight hours post transfection, the cells were lysed and the whole cell proteins ... is specific and does not alter the global cellular transcription Effect of VBARP siRNA on cell viability was tested by the trypan blue exclusion assay and cell viability assay The cell viability...
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Autoimmune Diseases – Contributing Factors, Specific Cases of Autoimmune Diseases, and Stem Cell and Other Therapies pdf
... helper cells The cytotoxic CD8+ T cells then affect beta -cell damage by releasing perforin and granzyme, and by Fas-mediated apoptosis In this way, macrophages, CD4+ T cells, and CD8+ T cells ... infiltrate showed that most cells corresponded to T cells, with T cytotoxic/suppressor cells being most abundant, although helper CD4+ T cells and NK cells Immunologic and Genetic Factors in Type ... and Stem Cell and Other Therapies disease initiation method, with a single and defined myelin peptide allows for the observation and measurement of changing T cell specificities over time (Vanderlugt...
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Báo cáo khoa học: ERK and cell death: ERK location and T cell selection pdf
... negative selecting ligand Note the separate location of pJNK and pERK, and Ras-GRP1 ⁄ Grb2 ⁄ SOS ⁄ Ras ⁄ Raf at the plasma membrane lead to such distinct cell fates as survival and death Much is ... problem Interestingly, JNK activity and subcellular location are the same regardless of the ligand strength, ERK location and T cell selection whereas ERK activity and location change depending of ... important for determining cell fate decisions in a diverse number of organisms and cell types (reviewed in [7,8]) In thymocytes, c-JunNH2-terminal kinase (JNK) [9], p38 [10] and extracellular signal-regulated...
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báo cáo hóa học:" Regression of orthotopic neuroblastoma in mice by targeting the endothelial and tumor cell compartments" pdf
... Roskilde, Denmark) NB cells (20,000/well), fibroblasts (15,000/well) and endothelial cells (5,000/well) (cultured in medium containing 10% serum) were added to the wells, and the cell survival index, ... 24, 48, and 72 h incubation period IC50 values were determined as CHS 828 concentrations with a survival index below 50% Cell morphology and cell death in vitro Morphological changes in NB cells ... concentrations of CHS 828 for 24, 48 and 72 h (concentration range: 0.1 nM – 10 μM) Cell survival was measured by FMCA Survival index was calculated as the percentage of viable cells at the actual concentration...
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báo cáo hóa học: " Quality of life among adolescents with sickle cell disease: mediation of pain by internalizing symptoms and parenting stress" pdf
... index, and disease severity) with parent- and teen-reported PPQ pain frequency, primary caregiver- and teen-reported BASC anxiety and BASC depression, PIP difficulty, and primary caregiver- and ... teacher-, and parent-report measure of adaptive and clinical functioning for children and adolescents ages 1/2 to 18 years of age The questions address emotions, behaviors, and self-perceptions and ... severity measured as sickle cell type.[13] Although pain and other sickle cell complications show an association with decrements in engagement in physical activities and in physical domains of...
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báo cáo hóa học:" Aberrant STYK1 expression in ovarian cancer tissues and cell lines" pdf
... estrogentumor cell Ovarian 2receptors lines express STYK1 and variably express Ovarian tumor cell lines express STYK1 and variably express estrogen receptors HS832 is a benign ovarian cell line and the ... benign, and malignant ovarian tissues To investigate the role of estrogen and GPR30 in STYK1 regulation, we treated a benign and several malignant ovarian cancer cell lines with estradiol and G-1 ... cancer cells based on microarray analysis and real-time PCR analysis [10] Estrogen receptors play a critical role in ovarian tumor cell growth Ovarian surface epithelial cells produce estradiol and...
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Using JavaTM ME Platform to Put the Fun into Your Mobile Device and Cell Phone pdf
... Command exitCommand = new Command("Exit", Command.EXIT, 99); /** * The Command objects appear as buttons in this example */ private Command toggleCommand = new Command("Toggle Msg", Command.SCREEN, ... Initialize the canvas and the commands */ public Hello() { myCanvas = new HelloCanvas(); myCanvas.addCommand(exitCommand); myCanvas.addCommand(toggleCommand); // you set one command listener to listen ... -// command fields /** * The button to exit the game */ private Command myExitCommand = new Command("Exit", Command.EXIT, 99); /** * The command to create a new maze (This command may appear...
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Báo cáo khoa học: "Radioprotective effects of fucoidan on bone marrow cells: improvement of the cell survival and immunoreactivity" doc
... marrow is the main cellular source for the hematopoietic and immune systems, it contains large numbers of lymphocytes, granulocytes, and stromal cells as precursors and mature cells Our results ... at a concentration of × 10 cells/ml in 6-well plates and treated with fucoidan (50 μg/ml) for 24 h and then irradiated once (1 Gy) After 24 h, the cells were harvested and stained for flow cytometric ... Preparation of cells and measurement of cell viability BMCs were harvested from the femurs and tibias of mice of C57BL/6 mice as described in previous report [9] Any contaminated red blood cells were...
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Báo cáo y học: "T-cell senescence and contraction of T-cell repertoire diversity – catalysts of autoimmunity and chronic inflammation" pdf
... T cells, and memory T cells turn over at a daily rate of 0.9–3.1% [25] In essence, adults need to produce 1.5 × 108 naive T cells and 1.5 × 109 memory T cells every day (Table 1) New naive T cells ... limited to the memory T -cell compartment and not affect the diversity of naive T cells because naive and memory T cells underlie different homeostatic control mechanisms and compete for different ... apoptosis in the responding T -cell population, and feedback control by inhibitory receptors and regulatory T cells Remarkably, diversity of the repertoire of naive and memory T cells has now been established...
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Báo cáo y học: "Association of FCGR2A and FCGR2A-FCGR3A haplotypes with susceptibility to giant cell arteritis" pdf
... dendritic cells, results in efficient MHC class I and II presentation [26] FcγRIIIa cross-linking additionally stimulates antigen-dependent cellular cytotoxicity by natural killer cells Page ... number not for citation purposes) Giant cell arteritis patients and controls This was an allelic association study where FCGR2A, FCGR3A, FCGR3B and FCGR2B alleles and pairwise haplotypes were examined ... GCA subjects and controls Linkage disequilibrium at the FCGR genetic locus Significant linkage disequilibrium was observed between FCGR2A and FCGR3A (D' = 0.31, P = 0.01) and FCGR3A and FCGR3B...
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Báo cáo khoa học: "In vitro characterization of cells derived from chordoma cell line U-CH1 following treatment with X-rays, heavy ions and chemotherapeutic drugs" pdf
... radiation or chemical treatment, cells were trypsinized and re-plated in P-100 cell culture dishes HeLa and U87-MG cells were cultured for 10 to 14 days, and U-CH1-N cells were kept in an incubator ... Figure Survival fraction of U-CH1-N cells after high LET heavy-ions A) X-rays and carbon-ions (LET 13, 30, 50, and 70 keV/μm), B) X-rays and neon-ions (LET 13, 70, and 120 keV/μm), C) X-rays and ... observed under microscope and colonies containing more than 50 cells were counted as survivors Cell survival assay was carried out to times independently Radiation exposed cell survival curves were...
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Báo cáo y học: "Hypoxia, hypoxia inducible factor and myeloid cell function" pdf
... nuclear and cytoplasmic (PHD3), and endoplasmic reticulum (P4H-TM) [29,30] PHDs and factor inhibiting HIF all display an absolute requirement for dioxygen, Fe(II) and 2-oxoglutarate, with PHD1 and ... hydroxylase activity and HIF signalling, as have the intracellular availability of iron and ascorbate and the local concentration of reactive oxygen species A role for sumoylation and histone acetylase ... glucose and high levels of reductive metabolites Hypoxia inducible factor and myeloid cell function In addition to the key role of HIF in regulating the cellular responses to hypoxia, work from Randall...
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Báo cáo y học: " Cellular stress-induced up-regulation of FMRP promotes cell survival by modulating PI3K-Akt phosphorylation cascades" pdf
... phosphorylation of Akt, a known cell survival- related signaling molecule Indeed, cell survival was compromised when FMRP levels were reduced and was Page of 15 prolonged in cells over-expressing FMRP ... regulators of cellular survival/ apoptosis control and activated Ras-PI3K-Akt signaling was regarded as a hallmark of many cancer cells [15,16], which are characterized by unregulated apoptosis and prolonged ... provided eGFPempty vector and eGFP-tagged FMRP vector Cell culture and treatment HeLa cells were cultured in DMEM containing 10% heat-inactivated FBS, 100 units/ml penicillin and 100 μg/ml streptomycin...
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báo cáo khoa học: " All-trans retinoic acid inhibits KIT activity and induces apoptosis in gastrointestinal stromal tumor GIST-T1 cell line by affecting on the expression of survivin and Bax protein" pdf
... GIST cells in term of inhibition of cell proliferation, and induction of apoptosis For the first time we have demonstrated that ATRA inhibited cell proliferation of GISTT1 and GIST-882 cell line ... durations, cells were harvested and fixed onto slides by using a cytospin (Shandon, Shandon Southern Products Ltd., Cheshire, UK) Cells then were stained with Wright-Giemsa solution Morphology of cells ... Effect of ATRA on cell proliferation of GIST-T1, GIST-882 and human normal fibroblast WI-38 cells GIST-T1, GIST-882 and human normal fibroblast WI-38 cells at a density of × 105 cells/ml were treated...
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báo cáo khoa học: " On-chip constructive cell-Network study (I): Contribution of cardiac fibroblasts to cardiomyocyte beating synchronization and community effect" pdf
... another three cell connection experiment That is, two cardiomyocyte cells were connected by a HeLa cell HeLa cells are one of the cell line and is not regarded as the connecting cells between ... of cells A and B, the beating of cell A stopped and then restarted in synchrony with the beating of cell B These results show that cardiomyocyte-fibroblast connections can couple a fibroblast and ... heterotypic strain cells in monolayer culture Exp Cell Res 1969, 58:420-6 Goshima K: Formation of nexuses and electrotonic transmission between myocardial and FL cells in monolayer culture Exp Cell Res...
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Báo cáo y học: "CTLA-4 blockade during dendritic cell based booster vaccination influences dendritic cell survival and CTL expansion" ppt
... %adjusted survival Page of (page number not for citation purposes) Journal of Immune Based Therapies and Vaccines 2007, 5:9 where adjusted %survival = 100 × ( %survival of Ag+ cells/(average % survival ... USA) and cultured at × 106 cells/ml in ml CM/well Fresh cytokines and medium were added on day Day DCs were harvested as non-adherent and loosely adherent cells These cells have previously been ... Hillcrest, England) and antibiotics The next day, non-adherent cells were harvested and resuspended in CM containing 10 ng/ml GM-CSF plus 20 ng/ml IL-4 (both from Peprotech, Rocky Hill, NJ, USA) and cultured...
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Báo cáo y học: "Comparative study between the Hybrid Capture II test and PCR based assay for the detection of human papillomavirus DNA in oral submucous fibrosis and oral squamous cell carcinoma" pdf
... for OSMF and OSCC, in which viral integration and the expression of viral oncogenes (E6 and E7) have been shown [8] HPV detection in potentially malignant and malignant oral squamous cell carcinoma ... was performed as per standard protocol and the tissue was processed by paraffin embedding 2-3 micrometer thick sections were cut and stained by haematoxylin and eosin (H and E) Biopsies, which ... (HC-II) and approved by the US food and drug administration (FDA) The performance characteristics of HC-II assay and the PCR for detection of HPV DNA have been compared in cervical lesions and results...
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