... counteracting ability ofthe osmolyte does not arise from the stabilization ofthe native state but arises primarily from the destabilization ofthe unfolded state oftheprotein in the presence of osmolyte ... 64 and 82% ofthe total FtsZ were pelleted in the absence and presence of 0.8 m TMAO, respectively Electron microscopy analysis ofthe FtsZ assembly reaction showed the formation of thicker and ... area of proteins andthe reduction in the solventaccessible surface area produces a decrease in the ligand-binding ability oftheprotein [52] An unfavorable interaction between proteinand osmolyte...
... higher protein content in their latex; and (2) the presence of other cysteine proteinases or isoforms in the latex To investigate these two hypotheses, theprotein concentration per milligram of ... for the first time, we are not aware ofthe frequency of tapping in other studies These and probably several other, as yet unknown, factors affecting the activity and concentration of latex proteins ... sequences ofthe proteins VXH-I, VXH-III, VXH-IVa and VXH-IVb are shown in Fig 6, and are compared with the known N-terminal sequences ofthe cysteine proteinases of C papaya and V cundinamarcensis The...
... from Hanes plots Determination ofthe metal contents ofthe Qor proteins, and detection ofthe nucleotide moiety ofthe molybdenum cofactor The contents of molybdenum and iron were determined by ... indicated in Fig 4B The absence of Mo(V)signals reveals the g2-component of FeSII It is noted that the g-factors ofthe FeSI and II signals ofthe Qor proteins from the wild-type strain and from P putida ... catalyse the synthesis and insertion of a cytidine dinucleotide cofactor into recombinant Qor as suggested by the release of CMP after hydrolysis ofthe enzyme, it appears that theassemblyof intact...
... basic protein, and thus the molecular mass oftheprotein reacted with the UL31 antiserum was consistent with that predicted These results indicate that the 35 kDa protein is the product ofthe ... egress of Alpha-, Beta-, and Grammherpesviruses [8,14,16-20] However, there is no report on the identification and characterization ofthe UL31 gene product of DEV In the present study, the UL31 ... threonine, and on tyrosine residues (Fig 1) Figure Prediction of Phosphorylation sites of deduced amid acids ofthe DEV UL31 protein Prediction of Phosphorylation sites of deduced amid acids of the...
... membrane fission event andthe final pinching off ofthe budding virus [4-6] In the last few years, late domain sequences were found to direct the interaction between the Gag proteins and some cellular ... support the hypothesis that the virus hijacks the MVB production system to direct the budding andthe release of virus particles [10] Recently, it was shown that HIV and MLV Gag polyproteins ... vicinity ofthe plasma membrane, they probably have been just released from the plasma membrane In the cells, very few labeled-Gag localized individually at the plasma membrane and most ofthe Gag proteins...
... supercomplexes andthe effect ofthe absence of intact complex I, but the results are too preliminary in this regard Heterologous expression ofthe ESSS-HA subunit The localization ofthe ESSS subunit in the ... panel) The signal from the 18 kDa subunit of complex I (NDUFB6) served as another identification ofthe complex at the position ofthe histochemical stain in the left panel Antisera against the SDHC ... revealed the existence of two additional subunits (bringing the total to 46), and one of these, the ESSS protein, is also encoded by an X-linked gene in humans and mouse [36] Most of these subunits...
... interpretation ofthe data suggests that the indicated basic region of ic3 ofthe D2 receptor and acidic region ofthe C-tail oftheD1 receptor might be involved in the interactions between the two ... dopamine D1and D2 receptor fusion proteins (D1 CFP and D2–YFP) in comparison with emission spectra ofthe cell popula- tions that co-express dopamine D1 receptor fusion protein (D1 CFP) and one ofthe ... colocalization in D1 ⁄ D1, D1 ⁄ D1MUT, D1 ⁄ D2 andD1 ⁄ D2R1 assays and partial colocalization in D1 ⁄ D2R2 assays D1 ⁄ D2R3 does not colocalize (B) HEK293 cells were cotransfected with D2–CFP and either...
... [9] The KIND domain in these proteins is localized to the N-terminal region, and their specific functional domains are located in the C-terminal region The C-lobe ofprotein kinases mediates the ... bacterially expressed proteins of these subregions (Fig S1C) and 1654 Fig Of all members ofthe KIND family of proteins, only KIND2 binds to MAP2 Pull-down assay (PD) ofthe endogenous MAP2 from ... cloned into pGEX-4T-2 The KIND domains of Spir-2 and PTPN13 were generated by RT-PCR and inserted into pEGFP-C1 and pGEX-4T-2 The N- and C-terminal regions ofthe v-KIND KIND2 andthe MAP2 CD were...
... conditions The ectodomains of both monomers in the dimer are required for this effect, suggesting that the epitope tag of one monomer is masked by the ectodomain ofthe other monomer in the dimer, and ... wedge-like structures to the N-terminal side ofthe D1, similar to the inhibitory wedge in RPTPa However, the crystal structures ofthe intracellular domains of LAR [19] and CD45 [20] suggest that ... oxidation induced changes in the conformation ofthe cytoplasmic domains of these RPTPs These results suggest there is flexibility in the cytoplasmic domain of RPTPs, and are evidence against rigid...
... longer-chain (and nonglobular) versions ofthe structure ofthe Bowman±Birk inhibitor in Fig The X-ray crystal structure pi2 reveals that the /,w angles of most ofthe residues ofthe Bowman±Birk ... helical conformation There may also be some b strand in some ofthe proteins, especially b- and j-casein judging by the wellde®ned negative ROA bands in these proteins in the range » 1245 cm)1, ... pi2) ofthe soybean variant of this protein, together with its ROA spectrum measured earlier [19] The general appearance ofthe ROA spectrum is quite similar to those ofthe caseins, synucleins and...
... GroEL from 15 and 30 lg ofthe cell lysate without (lanes and 3) or in the presence of ATP (lanes and 4) was detected with anti-UP12 Ig Samples (3 and lg of total proteins) ofthe cell extract ... 2), and one larger protein consisting of two UspA domains in tandem [17] The small members of this family are proteins of 142–144 amino-acids long; they are acidic and presumably located in the ... anti-GroEL Ig [27] The high yield ofthe UP12-GroEL complex isolation andthe ATP-mediated dissociation ofthe complex strongly support the suggestion that the two stress proteins GroEL and UP12 interact...
... which anchor theprotein in the membrane The position and function ofthe long C-terminal tail on the other hand remains a matter of controversy [10,11,13–15] According to one hypothesis, the hydrophilic ... description of recent advances in the understanding ofthe channel-, motor- and regulatory components ofthe Tic complex For reference, some ofthe available knowledge, including the proposed function of ... incoming preproteins Additionally, because the demand ofthe chloroplast is ‘sensed’ inside the organelle, the IEM is closest to the origin ofthe signal, and thus regulation at the Tic complex...
... for assemblyofthe iron– sulfur units It is of interest therefore to know whether the folding of these two proteins around their iron–sulfur cluster occurs similarly in mitochondria and in the ... assembly machinery of yeast mitochondria can build the inorganic unit of this normally cytosolic protein Furthermore, members ofthe extra-mitochondrial assembly line of these cofactors, the so-called ... mitochondria andthe other one is unknown Theprotein bands separated from purified aggregates on SDS ⁄ PAGE were cut and identified using MS The results indicated the presence of hIRP1, as expected, in band...
... 205 and 215 nm, indicating that Rv1399c is composed of 22% of a-helices, 25% of b-strand and 39% of random coil The catalytic triad is located at the bottom of a small ˚ ˚ pocket, 20 A length and ... experimentally Indeed, the presence of Arg213 along with the proximity ofthe charge relay Asp260 within the active site could be responsible for the modification ofthe catalytic properties ofthe catalytic ... by the amides of Ala163, Gly89 and Gly90, the latter’s being found in the HGGG consensus sequence motif characteristic of members ofthe HSL family [35] Our model shows that the architecture of...
... present in the other members ofthe APP superfamily, such as the absence ofthe exon encoding the KPI domain andthe lack of a second heparin-binding domain [3,4,13,26] Comparative analysis ofthe Xenopus ... in the appearance ofthe APLP1 and APLP2 gene families The fact that both mammals and Xenopus contain APP and APLP2 proteins suggests that the first gene duplication giving rise to the APP and ... sequence comparison between the Xenopus and mammalian APLP2 proteins (A) Alignment ofthe amino acid sequences of Xenopus APLP2-A/B, and human, mouse and rat APLP2 proteins The one letter amino acid...
... bound the junction Each formed two distinct complexes (Fig 2A) In the case ofthe E coli protein, the two complexes represent the binding of either a single tetramer of RuvA (complex I) or of two ... ofthe sequences forming the pin in the majority of bacteria with the exception of two Mycoplasma species and one of Ureaplasma In the RuvA proteins from these organisms the pin sequence is extended ... comparison ofthe binding of EcRuvA and MpRuvA to linear duplex DNA and shows the additional stability ofthe MpRuvA bound DNA complex compared to the EcRuvA bound DNA complex But the results...
... to that of PMT-C3 is that, at the sequence level, there is no discernible similarity of PMT-C3 to the proteins, with the exception ofthe Cryseobacterium protein glutaminase However, there is ... cellular outcomes, the sequence and structure ofthe activity domain of PMT differ considerably from those ofthe other dermonecrotic toxins [3] and point to a clear functional example of convergent ... this review, we focus on PMT and our current understanding ofthe structure–function, mechanism of action and cellular consequences of this newest member ofthe G -protein- deamidating dermonecrotic...
... structure are found at the end of strand D andthe beginning of strand E, i.e a further loss in D and a stabilization in E ofthe local b-structure geometry Compared with wt b2m, the cK58-b2m molecule ... [15,18] and requires the checking and redetermination of most ofthe resonance assignments ofthe molecule under investigation according to the standard methodology, i.e going through scalar and ... because ofthe ambiguous assignment of residues from these regions ofthe molecule Conformational heterogeneity of cK58-b2m and dK58-b2m The whole NMR dataset for cK58-b2m and dK58b2m revealed the...
... dependence ofthe heat capacity ofthe initial and final conformations (Cp,N2 and Cp,U), the heat capacity ofthe native state was taken to be a linear function of temperature and that ofthe unfolded ... between theproteinandthe buffer, it is reasonable to assume that the heat capacities of both the native and unfolded states depend upon neither the pH nor theprotein Ó FEBS 2004 Unfolding and ... of HU proteins from B stearothermophilus [20] and T maritima [18] The upper part ofthe figure shows the aligned amino acid sequences of each monomer within the homodimers, the ribbon models of...
... from the experiments shown in Figs and In each experiment the tissue with the highest expression level was set to 100 The height ofthe other bars indicates the relative amount of NICN1 mRNA in the ... amino acids The positions ofthe exon boundaries with respect to theprotein sequence are indicated by arrows Expression ofthe NICN1 gene To investigate the expression profile ofthe NICN1 gene ... dependent on the metabolic state ofthe organ As the RNA for the Northern blot andthe RT-PCR analysis had been derived from two different anonymous human donors the differences in the observed...